• KSBB Journal
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• 제24권3호
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• pp.279-286
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• 2009

Streptomyces is well known for their ability to synthesize enormous varieties of antibiotics as secondary metabolites. Among them, S. avermitilis produces avermectins, a group of antiparasitic agents used in human and veterinary medicine. However, S. avermitilis also produces oligomycin, which is a potential toxic inhibitor of oxidative phosphorylation in mammalian cells. Therefore, we decided to disrupt oligomycin synthetase gene to prevent co-production of oligomycin in S. avermitilis. To create plasmid for disruption, the smallest gene of oligomycin synthetase gene cluster was obtained by PCR from S. avermitilis chromosome. Then, apramycin resistance gene was inserted in oligomycin synthetase gene for selection. After transformation of this plasmid, oligomycin synthetase gene (olmA5) in the chromosome was displaced with disruption cassette on the plasmid via homologous recombination. As a result of this gene replacement, we obtained mutants (olmA5::apra) that no longer makes the toxic oligomycin. And the mutants confirmed by PCR and HPLC analysis. However, showed no increasement of avermectin production in the mutant was observed.

• Journal of the Korean Geotechnical Society
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• 제21권10호
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• pp.41-48
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• 2005

The gravitational consolidation behavior of the dredged ground with horizontal drains is analyzed using a finite difference program developed for self-weight consolidation analysis with horizontal drains. The influence of area and direction of horizontal drains on the consolidation time and settlement is analyzed. Various field conditions such as the non-treated ground below horizontal drain installed ground, the accumulation of drained water at the end of horizontal drains, are also included in the analyses. It was found that a slight decrease of consolidation time is resulted in the twice increase of the sectional area of drains. Installing drains vertically can reduce the consolidation time more significantly than installing drains horizontally. The analyses showed quantitatively that the non-treated ground below the horizontal drain installed ground has much influence on long term consolidation settlement, and the accumulation of hydraulic head at the end of horizontal drains results in the increase of consolidation time and insufficient consolidation.

• Korean Chemical Engineering Research
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• 제55권1호
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• pp.1-6
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• 2017

More addition of calcium carbonate in printing paper allows savings of the wood fibers and the drying energy. Pre-flocculation of GCC (ground calcium carbonate) using functional polymers was known as the best available technology to make high loaded paper until now, and it allowed less reduction of the paper essential properties such as tensile strength and smoothness at higher GCC content. However, pre-flocculated GCC became unstable in size under the continued agitation in the mill. Therefore, pre-flocculation method was modified in such a way that the in-situ calcium carbonate was formed between the GCC particles of the pre-flocculated GCC, and the resultant became more stable in size, which we named as HCC (hybrid calcium carbonate). HCC turned out to make high tensile strength and smoothness as much as the pre-flocculated GCC and gave much better size stability against stirring. Furthermore, HCC gave high bulk that pre-flocculation could not make.

• Journal of the Korean Society of Propulsion Engineers
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• 제18권3호
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• pp.70-77
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• 2014

In this paper, the development of unique model of the 2.75 inch rocket propulsion system is described. Recently developed korean 2.75 inch rocket propulsion system shows the improvement of a flame stability resulted from a change in the configuration of propellant grain, and of an incidental ignition protection function using the EMI(electromagnetic interference) filter on ignition system. Moreover it is shown that a directional flight stability is improved by increasing the number of fins and changing the nozzle configuration. Static firing test and thermal shock test were conducted for the validation before flight, and flight test of 210 rounds of rockets was conducted to verify the trajectory uniformity. In addition, intellectual property issues can be overcome with the unique korean 2.75 inch rocket motor as well as the performance improvement.

• Journal of the Korean Wood Science and Technology
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• 제27권3호
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• pp.7-16
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• 1999

This research was carried out to compare structure, equilibrium moisture content(EMC), adsorption and acoustical properties between 4 retention levels of saligenin(o-hydroxybenzyl alcohol) treated, heat-treated and untreated chestnut(Castanea crenata S. et Z.) and paulownia(Paulownia spp.) woods. The cell structures of untreated wood and heat-treated wood were not different but the cell wall of saligenin treated wood was thicker than that of untreated wood for both species. EMC of untreated wood was the highest, next was that of heat-treated wood, and that of saligenin treated wood was the lowest, and among saligenin treated woods, EMC was decreased as retention level increased for both species. Adsorption curves were parabolically increased. Acoustical properties of saligenin treated wood were most improved, and the next were those of heat-treated wood. Comprehensive acoustical property was improved at retention level 20% and 30% for chestnut wood, and at retention level 80% for paulownia wood.

• Journal of the Korean Wood Science and Technology
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• 제27권3호
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• pp.31-38
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• 1999

This paper deals with a coating technique of hardening surface layer of softwood{Larix leptolepis) flooring board to improve its surface properties such as hardness and abrasion resisitance without spoiling its exellent qualities such as beautiful color tones and great strength. For surface hardening of the wood, polyurethane wood sealer was used as under coat, polyurethane sanding sealer as intermediate coat, and UV-curing epoxy acrylate and urethane acrylate varnishes as top coat. The hardness of the wood was improved by the coating treatment and it was similar to that of high density hardwood such as oak, keruing. The abrasion resistance of the coated wood was greatly improved by UV curing epoxy acrylate or urethane acrylate varnishes. Adhesion properties and impact resisitance of the coated wood surface were also good. It was suggested that the well-coated larch wood could be used as interior flooring board as substitute for hardwood such as keruing.

• Geotechnical Engineering
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• 제4권1호
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• pp.17-28
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• 1988

Among several types of element tests to predict soil behalf.iota in a laboratory, the torsion shear apparatus, in which the directions of principal stresses could be rotated during shearing, wra explained. In this study, this torsion shear apparatus was improved so as to be used in tests on clay specimens . And some undrained torsion shear tests u.ere performed on remolded specimens of Ko-consolidated clay to investigate the influence of reorientation of the principal stress directions on the stress-strain behavior The soil behavior by the torsion shear apparatus without torque was compared It.ith that by the conventional triaxial compression tests . The stress path, provided by both vertical loads and torque during torsion shear tests, has much effect on the stress-strain behavior, the pore pressure and the effective principal stress ratio . The rotation angle of the principal stress and the b-value were gradually increased with increasing shear strain, but converged to the values at failure.

• Geotechnical Engineering
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• 제11권2호
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• pp.99-106
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• 1995

Although lots of experimental studies of coal ash have been performed to study the utilization as construction materials, the environmental characteristics of coal ash are still qestionable. In this study, fly ash is examined to be classified according to Korean Environmental Standard and analized whether the batch test results are within the toler trance limit when utilized or treated as reclamation and earth work materials. The batch tests was performed to examine pH and contaminant contents. Consequently, fly ash is classified as non hazardous industrial waste. The pH value shows a strong alkalinity than the tolerance limit, but it is implied that fly ash can be used to neutralize the acid ground. All other items except pH satisfy the tolerance limit, In addition, a small quantity of additives(cement) which used to improve the poor geotechnical properties of coal ash, could decrease the pH value into the tolerance limit as well as improve strengtIL durability and permeability. It is concluded that when coal ash is used properly, there is no enviormental harmfulness as construction materials.

• Journal of the Korean Geotechnical Society
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• 제34권6호
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• pp.37-47
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• 2018

In this study, 34 laboratory load test data were collected, and analyzed to propose the equations for predicting ultimate bearing capacity of sand compaction pile (SCP) and gravel compaction pile (GCP) reinforced clay. The collected data were compared with the ultimate bearing capacity estimated by existing theoretical equations, and the prediction accuracy of the existing theoretical equations was identified. Also, multiple regression analysis was performed to predict the ultimate bearing capacity, and the most efficient number and type of input variables were selected through error evaluation by leave-one-out cross validation. Finally, the multiple regression equations for estimating the ultimate bearing capacity of laboratory load test for SCP and GCP were proposed, and their performance was evaluated.

• KSBB Journal
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• 제9권2호
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• pp.191-199
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• 1994

Protoplasts of both strains were produced by lysozyme digestion at $30^{\circ}C$ for 180min. Both strains were treated with $40{\mu}g$/ml of lysozyme in 30mM Tris-HCl buffer(pH 7.5) containing 10% sucrose at the late logarithmic growth phase. It was found that the efficiency of protoplast formation was high at $30^{\circ}C$ and pH 7.5 by measuring the decrease in absorbance. Optimum concentrations of sucrose $Ca^{2+}, \;Mg^{2+}$ for protoplast formation were determined to be 15%, 20mM and 6mM, respectively. Hydrolysis of cell wall and protoplast formation efficiency for L. plantarum showed better results than those for Leu. mesenteroides. The resistances to antibiotics erythromycin and chloramphenicols were chosen as the selection marker for the fusant between L. plantarum and Leu. mesenteroides. Production phase of protoplast in Leu. mesenteroides was also compared with L. plantarum in this paper.