• Journal of Life Science
• /
• v.24 no.12
• /
• pp.1269-1275
• /
• 2014

TALEN is a newly developed gene engineering method to knock out specific genes. It contains a DNA binding domain and a Fok1 nuclease domain in the TALEN plasmid. Therefore, the engineered TALEN construct can bind to any region of genomic DNA and cut the target nucleotide, thereby inducing mutation. In this study, we constructed two TALEN constructs targeted to a protein initiation codon (DBEX2) or the 25th upstream region (DBPR25) to enable mRNA synthesis of SETDB1 HMTase. We performed the TALEN cloning in two steps. The first step was from module vectors to pFUS array vectors. We confirmed successful cloning with a colony PCR experiment and Esp31 restriction enzyme digestion, which resulted in a smear band and a 1 Kb insert band, respectively The second step of the cloning was from a pFUS array vector to a mammalian TALEN expression vector. The engineered TALEN construct was sequenced with specific primers in an expression vector. As expected, a specific array from the module vectors was shown in the sequencing analysis. The specific module sequences were regularly arrayed in every 100 bp, and SETDB1 expression totally disappeared in the TALEN-DBEX2 transfection. PCR amplification targeting of DBEX2 was performed, and the PCR product was digested with a T7E1 restriction enzyme. The expression of SETDB1 was down-regulated in the TALEN-DBPR25 transfection. Morphological changes were also observed in the two TALEN constructs with transfected HeLa cells. These results suggest that the engineered TALEN constructs in two strategic approaches are very useful to knock-out of the SETDB1 gene and to study gene function.

• The Journal of the Convergence on Culture Technology
• /
• v.9 no.4
• /
• pp.95-103
• /
• 2023

This paper proposes an artificial intelligence convergence education program for teaching the main concept and principle of Support Vector Machines(SVM) at elementary schools. The developed program, based on Jeju's natural environment theme, explains the decision boundary and margin of SVM by vertical and parallel from 4th grade mathematics curriculum. As a result of applying the developed program to 3rd and 5th graders, most students intuitively inferred the location of the decision boundary. The overall performance accuracy and rate of reasonable inference of 5th graders were higher. However, in the self-evaluation of understanding, the average value was higher in the 3rd grade, contrary to the actual understanding. This was due to the fact that junior learners had a greater tendency to feel satisfaction and achievement. On the other hand, senior learners presented more meaningful post-class questions based on their motivation for further exploration. We would like to find effective ways for artificial intelligence convergence education for elementary school students.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.12 no.8
• /
• pp.3617-3625
• /
• 2011

This paper introduces new concept elevator door system with linear induction motor(LIM) featuring simple structured and direct drive mechanism, which makes up for the weak points of the conventional complex structured door system. The optimal configuration of linear door system and the design results of linear LIM will be proposed in this paper and the validation of LIM design results will be investigated through the test. In this study, high performance of drive controller applied vector control and optimum drive pattern also are suggested. In addition, this paper describes details of designing and manufacturing process of linear door system and investigates the performance by analysis and test. The analytical and experimental results of this paper are considered as the productive data for the development of new elevator door system.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.13 no.2
• /
• pp.237-242
• /
• 2003

In this paper, we propose a new adaptive intrusion detection algorithm based on clustering: Kernel-ART, which is composed of the on-line clustering algorithm, ART (adaptive resonance theory), combining with mercer-kernel and concept vector. Kernel-ART is not only satisfying all desirable characteristics in the context of clustering-based IDS but also alleviating drawbacks associated with the supervised learning IDS. It is able to detect various types of intrusions in real-time by means of generating clusters incrementally.

• The Journal of Korean Association of Computer Education
• /
• v.10 no.1
• /
• pp.97-105
• /
• 2007

In this paper, visualization of convolution operation is presented, which is implemented by scalable vector graphics (SVG). Convolution operation is one of the basic essential concepts in the area of signal and image processing. However, it is difficult for students to intuitively understand the operation of convolution since it is mainly based on mathematical representation. We present the visualization of convolution operation and its applications which are implemented by SVG. The effects of the proposed approach have been analyzed by interviews. It has been seen that the proposed visualization of convolution operation could be effectively applied to learn the convolution operation and its applications.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.41 no.3
• /
• pp.17-24
• /
• 2004

In this paper, the wavelet video coding using Low-Band-Shift(LBS) method and multiresolution motion estimation(MRME) is proposed. To overcome shift- variant property on wavelet coefficients, the LBS was proposed. LBS method previously has superior performance in terms of rate-distortion characteristic. However, this method needs more memory and computational complexity. Therefore to reduce computational complexity of video coding using LBS, we combine MRME with LBS. When mm is applied only, it has 7 times as much as existing method's motion vector because each subband has different motion vector using property of LBS, number of motion vector decreases. Proposed method decreases motion vector, and it decreases motion compensated Prediction error by detailed motion estimation. And then it shows better coding performance. Also this method reduces computational amount by smaller search area in higher resolution. The computational complexity of the proposed method is 12.1% of that of existing method at 3-level wavelet transform. The experimental results with the proposed method show about 0.2∼9.7% improvement of MAD performance in case of lossless coding, and 0.1∼2.0㏈ improvement of PSNR performance at 4he same bit rate in case of lossy coding.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.7 no.2
• /
• pp.149-157
• /
• 2002

In this paper, a novel overmodulation strategy for space-vector PWM(SVPWM) inverters to utilize dc link voltage fully Is presented. The proposed strategy uses the concept of SVPWM based on the zero sequence signal(offset voltage) injection principle. So, by modifying the pole voltage simply, the linear control of inverter output voltage over the whole overmodulation range can be achieved easily The proposed strategy is so simple that its practical implementation is easy. The validity of the proposed strategy is confirmed by the experimental results.

• The Journal of the Acoustical Society of Korea
• /
• v.18 no.7
• /
• pp.17-22
• /
• 1999

In this paper we propose a new mixed method of LDA and tri-tone model to predict Korean prosodic break indices(PBI) for a given utterance. PBI can be used as an important cue of syntactic discontinuity in continuous speech recognition(CSR). The model consists of three steps. At the first step, PBI was predicted with the information of syllable and pause duration through the linear discriminant analysis (LDA) method. At the second step, syllable tone information was used to estimate PBI. In this step we used vector quantization (VQ) for coding the syllable tones and PBI is estimated by tri-tone model. In the last step, two PBI predictors were integrated by a weight factor. The proposed method was tested on 200 literal style spoken sentences. The experimental results showed 72% accuracy.

• Journal for History of Mathematics
• /
• v.17 no.2
• /
• pp.61-72
• /
• 2004

In this article we study on didactic transposition and enlargement of the Ceva theorem(if three cevians AX, BY, CZ, one through each vertex of a triangle ABC, are concurrent, then $\frac{BX}{XC}\frac{CY}{YA}\frac{AZ}{ZB}$ = 1). We suggest inverse of the Ceva theorem, some different forms of the Ceva theorem(oriented segment form, trigonometric form, vector form), enlarged the Ceva theorem of polygon and tetrahedron, and in detail propose these proofs.

• Proceedings of the KIEE Conference
• /
• 2003.04a
• /
• pp.154-156
• /
• 2003

3상 4레그(leg) VSI(Voltage Source Inverter)는 독립된 3개의 출력전압을 합성할 수 있다. 따라서 전압 합성에 있어서 3개의 자유도를 갖는다. 본 논문에서는 3상 4레그 VSI를 위한 PWM방법에 대해서 설명한다. 3상 4레그 VSI를 위한 기존의 PWM기술은 3-D SVPWM으로 그 구현 방법에 있어서 많은 양의 계산과 테이블을 필요로 하였다. 하지만, 본 논문에서 제안 한 방법은 3상 3레그 VSI경우에 적용되었던 옵셋(offset) 전압의 개념을 사용한다. 또한 단일 반송파를 이용하여 폴(Pole)전압과 비교함으로써 PWM을 구현하기 때문에 그 적응이 매우 간단하다. 본 논문에서는 기존의 3-D SVPWM과 제안한 방법이 해석적으로 동일한 방법임을 증명하였으며, 3상 4레그 VSI에서 합성가능한 3자유도의 전압범위를 계산하였다. 마지막으로 이를 실험을 통하여 검증하였다.