• Restorative Dentistry and Endodontics
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• v.30 no.5
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• pp.393-401
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• 2005

The purpose of this study was to compare the canal configuration after shaping by ProTaper rotary files and ProTaper hand files in resin simulated canals. Forty resin simulated canals with a curvature of J-shape and S-shape were divided into four groups by 10 blocks each Simulated root canals in resin block were prepared by ProTaper rotary files and ProTaper hand files using a crown-down pressureless technique All simulated canals were prepared up to size $\#25$ file at end-point of preparation. Pre- and post-instrumentation images were recorded with color scanner. Assessment of canal shape was completed with an image analysis program. Measurements were made at 0, 1, 2, 3, 4, 5, 6 and 7 mm from the apex. At each level, outer canal width, inner canal width, total canal width, and amount of transportation from original axis were recorded. Instrumentation time was recorded. The data were analyzed statistically using independent t-test. The result was that ProTaper hand files cause significantly less canal transportation from original axis of canal body and maintain original canal configuration better than ProTaper rotary files, however ProTaper hand files take more shaping time.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.5
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• pp.741-749
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• 2022

Major space agencies are planning for the rover-based lunar exploration since water-ice was detected in permanently shadowed regions (PSR). Although sunlight does not directly reach the PSRs, it is expected that reflected sunlight sustains a certain level of low-light environment. In this research, the indoor testbed was made to simulate the PSR's lighting and topological conditions, to which low light enhancement methods (CLAHE, Dehaze, RetinexNet, GLADNet) were applied to restore image brightness and color as well as to investigate their influences on the performance of feature extraction and matching methods (SIFT, SURF, ORB, AKAZE). The experiment results show that GLADNet and Dehaze images in order significantly improve image brightness and color. However, the performance of the feature extraction and matching methods were improved by Dehaze and GLADNet images in order, especially for ORB and AKAZE. Thus, in the lunar exploration, Dehaze is appropriate for building 3D topographic map whereas GLADNet is adequate for geological investigation.

• Composites Research
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• v.36 no.5
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• pp.355-360
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• 2023

Epoxy-based carbon fibers reinforced plastic (CFRP) exhibit limitations in their suitability for industrial applications due to high brittleness characteristics. To address this challenge, extensive investigations are underway to enhance their toughness properties. This research focuses on evaluating the toughening mechanisms achieved by Polyamide 6 particles(p-PA6) and Carboxyl-Terminated Butadiene-Acrylonitrile (CTBN) elastomer, with a specific emphasis on utilizing minimal additive quantities. The study explores the impact of varying concentrations of p-PA6 and CTBN additives, namely 0.5, 1, 2.5, and 5 phr, through comprehensive Mode I fracture toughness and tensile strength analyses. The inclusion of p-PA6 demonstrated improvements in toughness when introduced at a relatively low content of 1phr. This improvement manifested as a sustained fracture behavior, contributing to enhanced toughness, while simultaneously maintaining the material's tensile strength. Furthermore, the investigation revealed that the incorporation of p-PA6 affected in particle aggregation, thus influencing the overall toughening mechanism. Incorporation of CTBN, an elastomeric modifier, exhibited a pronounced increase in fracture toughness at higher concentrations of 2.5 phr and beyond. However, this increase in toughness was accompanied by a reduction in tensile strength, resulting in fracture behavior similar to conventional CFRP exhibiting brittleness. The synergy between pPA6, CTBN and CFRP appeared to marginally enhance tensile strength under specific content conditions. As a result of this study, optimized conditions for the application of the p-PA6, CTBN toughening technology have been identified and established.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.17-17
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• 2001

착상전 수정란 단계에서 형질전환 수정란의 선발은 형질전환동물의 효율을 증대시킬 수 있는 방법이다. 성공적인 형질전환동물의 생산을 위해서는 생산된 수정란의 mosaicism 빈도를 감소시켜 전체 할구에서의 유전자 발현을 유도하는 것이 최적일 것이다. 따라서 본 연구에서는 돼지의 웅성 생식세포를 이용한 형질전환동물의 생산에 있어서 다양한 정자세포 이용시 형질전환 수정란의 생산성 및 mosaicism 빈도를 조사하였다. 아울러 돼지 웅성생식세포내 GFP 유전자도입시 세포들의 생존율 및 원형정자세포분리 후 배양에 따른 형태적 변화를 관찰하였다. 돼지의 웅성 생식세포내 GFP 유전자 도입은 전기자극법 (1.3 ㎸/cm, 200 $\mu\textrm{s}$) 에 의하여 수행되었으며, 이 때 생존율은 60-70%이였다. 유전자가 도입된 전체 세포중 원형정자세포군의 분리는 유식세포분리기에 의하여 수행하였으며, 전체집단에 대한 분리군의 비율은 평균 16.2%이였다. 형질전환 수정란의 생산은 정자 (ICSI), 원형정자세포 (ROSI), 배양후 확장된 원형정자세포(ELSI)를 이용하였으며 각각의 난할율은 ICSI (82.9%), ROSI (59.1%), ELSI (62.1%)로 유의한 차이를 나타내었다. 그리고 8세포기까지의 배발달율은 각각 61.1, 40.9 및 48.6%이였으며, 상실배 및 포배기형성율은 각각 24.6, 18.1 및 32.4%이였다. 형광현미경하에서 GFP 단백질이 발현된 8세포기 수정란을 대상으로 각각의 할구를 primer extension pream-plification (PEP) PCR 방법으로 분석한 결과, ICSI 및 ROSI 실시후 대부분 (15/20, 9/10) 의 수정란은 3~4개의 할구에서만 GFP 유전자의 존재여부를 확인할 수 있었으며, 전체 할구에서 GFP 유전자가 모두 확인된 수정란은 없었다. 반면에 배양된 확장 원형정자세포를 이용하여 생산한 수정란의 경우, 4/10 (40%)에서 전체 할구내에 GFP 유전자의 존재를 확인할 수 있었다. 이러한 결과는 비록 배발달율 및 GFP 유전자 발현율에 있어서는 ELSI방법이 ICSI 등의 방법보다 현저히 낮았지만, mosaicsism 빈도가 낮아 바람직한 형질전환 수정란 생산에서는 오히려 유용한 방법이라고 사료된다. 또한 외래 유전자의 도입효율 면에서 후기 원형정자나 성숙정자보다 초기 원형정자세포에 외래유전자를 도입한 다음, 성숙시킨 확장원형 정자세포를 이용하는 방법이 보다 우수하다는 것을 시사하였다. 따라서 본 연구결과는 포유동물의 웅성 생식세포를 이용하여 nonmosaicisn을 나타내는 형질전환수정란을 생산하고 선발할 수 있는 일련의 기술적 과정을 정립하였다고 사료된다.

• Horticultural Science & Technology
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• v.32 no.4
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• pp.454-462
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• 2014

This study was carried out to determine the effect of tree height on light transmission, spray penetration, tree growth performance, fruit quality attributes, and labor productivity in the slender-spindle system of 'Hongro'/M.9 apple trees. With increasing tree height, the light penetration into the internal parts of the canopy decreased, especially in the lower canopy. Leaf area index (LAI) increased with increasing tree height, thereby leading to a reduction in the extent of spray penetration into the interior of the canopy. With increasing tree height, shoot growth was more vigorous but produced slender shoots in the upper canopy compared to the lower canopy. Although the soluble solid content and coloration of fruit decreased, there was no difference in fruit firmness and acidity. In addition, the number of final fruit set increased, although the production of large fruit (> 305 g) decreased. The increase in tree height also significantly increased the labor required for practices such as thinning of flowers and fruits, pruning, and harvesting. Nevertheless, this problem of increased in labor input in taller trees would was eased by use of a mechanical lift. Utilizing a lift for thinning the flowers of trees 4.5 m in height saved 14.6 min per tree, compared to the use of ladder. Therefore, it is highly considerable that in order to enhance light transmission and fruit coloration, light conditions should be improved in the internal tree canopy of slender-spindle systems.

• Journal of the Korean GEO-environmental Society
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• v.9 no.4
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• pp.23-36
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• 2008

Soil nailing system can be mentioned to a method of supporting as the shear strength of in-situ soils is increased by passive inclusions. In the general soil nailing system, facing walls are used in two kind of a lattice concrete block or a cast in placed concrete wall. A case of lattice concrete blocks is used in slow slopes greater than 1(V):0.7(H). Also, a case of a cast in placed concrete wall is used in steep slopes less than 1(V):0.5(H). The cast in placed concrete walls are constructed to 30 cm thick together with a shotcrete facing. In this study, the assembling soil nailing method as a new soil nailing system will be proposed. This method is assembly construction using precast concrete panels with 20 cm thick. So, the ability of construction and the quality of facings can be improved more than a conventional soil nailing system. This method can be obtained the effects that a global slope stability increase, as precast concrete panels are immediately put on cutting face after excavating a slope. In this study, confining effects of concrete panels using the assembling soil nailing system were found out by large scaled load tests. In the tests, the load-settlement relationship to an assembling soil nailing system due to the stiff facings as concrete panels appeared to be better than a typical soil nailing system with shotcrete facings.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.29 no.4
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• pp.137-147
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• 2011

This study aims to find out the construction process and planning characteristics of Suwon and Hwa-sung in 18th century from the point of water system. This study has an explanatory approach. The planning intents in the water system was driven out by analyzing various planning elements in relation to water system in the process of constructing Hwa-sung in 18th century. Using Entire Map of Hwa-sung, land registration map made in 1911 and topographic map of 1/10,000 scale made in 1917, water system and interpretation of spatial structure in Hwa-sung were analyzed. The results are as follows(Planning characteristics of the water system in Haw-sung in 18th century are as follows). Firstly, in determining the spatial structure and location of Hwa-sung, water system had an important role. Secondly, integrated drainage system was planned by the organization of natural and artificial water stream. Thirdly, the main street system and land use structure were planned in relation with water system. Fourthly, territoriality of main area was planned with water stream. Fifthly, ponds were constructed for flood control and they had important role as landscape elements. Sixthly, water stream was used as intentional BiBo element. As a result of the study, the weater system of Hwa-sung in 18th century was planned by the organization of natural and artificial water stream in relation with the location of new town and wide area's spatial structure, street system, land use structure, territoriality of main area, terriflood control, water quality protection, landscape, 비보 and urban daily life.

• Clinical and Experimental Reproductive Medicine
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• v.34 no.3
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• pp.179-188
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• 2007

Objectives: Many neurological diseases are known to be caused by expansion of trinucleotide repeats (TNRs). It is hard to diagnose the alteration of TNRs with single cell level for preimplantation genetic diagnosis (PGD). In this study, we describe methods optimized for PGD of TNRs related diseases such as Huntington's disease (HD), spinocerebellar ataxia 3 (SCA3) and fragile X syndrome (FXS). Methods: We performed the preclinical assays with heterozygous patient's lymphocytes by single cell PCR strategy. Fluorescent semi-nested PCR and fragment analysis using automatic genetic analyzer were applied for HD and SCA 3. Whole genome amplification with multiple displacement amplification (MDA) method and fluorescent PCR were carried out for FXS. Amplification and allele drop-out (ADO) rate were evaluated in each case. Results: The fluorescent semi-nested PCR of single lymphocyte showed 100.0% of amplification and 14.0% of ADO rate in HD, and 94.7% of amplification and 5.6% of ADO rate in SCA3, respectively. We could not detect the PCR product of CGG repeats in FXS using the fluorescent semi-nested PCR alone. After applying the MDA method in FXS, 84.2% of amplification and 31.3% of ADO rate were achieved. Conclusions: Fluorescent semi-nested PCR is a reliable method for PGD of HD and SCA3. The advanced MDA method overcomes the problem of amplification failure in CGG repeats of FXS case. Optimization of methods for single cell analysis could improve the sensitivity and reliability of PGD for complicated single gene disorders of TNRs.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.38 no.4
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• pp.12-24
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• 2020

The purpose of this study is to analyze the influence of the water system on the location, spatial structure, and construction method of Hongju-eupseong, centering on Hongjumok-eupchi. During the Joseon Dynasty, the water system in Hongjumok-eupchi is composed of artificially constructed Seong-an Runnel and ponds based on a branch-shaped natural waterways flowing from south to north and west to east. Compiling the results of various literature records, excavations and analysis of map data, it can be seen that the water system has an important influence on the construction of Hongju-seong. Firstly, Hongju-seong from the Goryeo Dynasty to the late Joseon Dynasty is located using a circular shape of topographical structure and a small erosion basin formed on the inner side of the Hongseongcheon and Wolgyecheon streams without significant change in location. In particular, Wolgyecheon and Hongseongcheon are natural moats, which are harmonized with Sohyangcheon and riverside topographical structures, affecting the location and construction method of Hongju-seong, water related facilities, and the spatial structure of eupseong. It is understood that location characteristic of Hongju-seong reflects the urban location structure harmonized with waterways in ancient China and Korea. Secondly in harmony with the water system and topographic structure of Hongju-seong, it is an important factor in deciding the land use of the town, the arrangement of the town hall facilities and inducing various non-subsidiary measures such as the establishment of embankment forest with a secret function and the closure of the south gate. In addition, artificial drainage facilities such as Seongan runnel and ponds are being actively introduced from early on to protect the walls or towns from flooding of Wolgyecheon. Especially there were typical methods for protecting the walls from water damage such as the Joseon Dynasty stone castle structure that was integrated with saturn(soil wall) in the Goryeo Dynasty, retreating wall in the northern gate area in the late Joseon Dynasty, and the method of constructing wall using korean tile and stone floors between reinforced soil layers in the western and northern wall.

• Journal of Internet Computing and Services
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• v.16 no.2
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• pp.77-83
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• 2015

Frequent pattern mining, which is one of the major areas actively studied in data mining, is a method for extracting useful pattern information hidden from large data sets or databases. Moreover, frequent pattern mining approaches have been actively employed in a variety of application fields because the results obtained from them can allow us to analyze various, important characteristics within databases more easily and automatically. However, traditional frequent pattern mining methods, which simply extract all of the possible frequent patterns such that each of their support values is not smaller than a user-given minimum support threshold, have the following problems. First, traditional approaches have to generate a numerous number of patterns according to the features of a given database and the degree of threshold settings, and the number can also increase in geometrical progression. In addition, such works also cause waste of runtime and memory resources. Furthermore, the pattern results excessively generated from the methods also lead to troubles of pattern analysis for the mining results. In order to solve such issues of previous traditional frequent pattern mining approaches, the concept of representative pattern mining and its various related works have been proposed. In contrast to the traditional ones that find all the possible frequent patterns from databases, representative pattern mining approaches selectively extract a smaller number of patterns that represent general frequent patterns. In this paper, we describe details and characteristics of pattern condensing techniques that consider the maximality or closure property of generated frequent patterns, and conduct comparison and analysis for the techniques. Given a frequent pattern, satisfying the maximality for the pattern signifies that all of the possible super sets of the pattern must have smaller support values than a user-specific minimum support threshold; meanwhile, satisfying the closure property for the pattern means that there is no superset of which the support is equal to that of the pattern with respect to all the possible super sets. By mining maximal frequent patterns or closed frequent ones, we can achieve effective pattern compression and also perform mining operations with much smaller time and space resources. In addition, compressed patterns can be converted into the original frequent pattern forms again if necessary; especially, the closed frequent pattern notation has the ability to convert representative patterns into the original ones again without any information loss. That is, we can obtain a complete set of original frequent patterns from closed frequent ones. Although the maximal frequent pattern notation does not guarantee a complete recovery rate in the process of pattern conversion, it has an advantage that can extract a smaller number of representative patterns more quickly compared to the closed frequent pattern notation. In this paper, we show the performance results and characteristics of the aforementioned techniques in terms of pattern generation, runtime, and memory usage by conducting performance evaluation with respect to various real data sets collected from the real world. For more exact comparison, we also employ the algorithms implementing these techniques on the same platform and Implementation level.