• Title/Summary/Keyword: 간접측정법

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The Effects of MERS(Middle East Respiratory Syndrome) Event on the Psychosocial Wellbeing of Healthcare Workers and the Public with the Mediating Effect of Resilience (메르스(Middle East Respiratory Syndrome)사태에 따른 병원종사자와 일반인의 스트레스 정도가 사회심리적 건강에 미치는 영향과 회복탄력성의 매개효과)

  • Kwon, Hyuk-Min;Kim, Tae-Hyung;Choi, Mal-Rye;Kim, Byoung-Jo;Kim, Hyoung-Wook;Song, Ok-Sun;Eun, Hun-Jeong
    • Korean Journal of Psychosomatic Medicine
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    • v.25 no.2
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    • pp.111-119
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    • 2017
  • Objectives : The purpose of this study is to investigate the effects of stress on psychosocial wellbeing at the time of an outbreak of Middle East respiratory syndrome(MERS) and to investigate the effect of resilience as a mediator on the relationship between stress and psychosocial wellbeing. Methods : Perceived Stress Scale, Psychosocial Wellbeing Index Short Form, and the Conner-Davidson Resilience Scale was implemented for 156 medical persons who worked at the hospital in which exposure to MERS cases had been confirmed and 127 ordinary people. We conducted a Pearson correlation coefficient and a hierarchical multiple regression to confirm the effect of stress on psychosocial wellbeing and the mediating effect of resilience between stress and psychosocial wellbeing. Results : The higher the perceived stress, the lower the psychosocial wellbeing in both healthcare workers and the public. The higher the perceived stress, the lower the resilience and the research results showed that there was a partially mediating effect of resilience in the relationship between stress and psychosocial wellbeing. Conclusions : This study demonstrated that the degree of individual resilience can indirectly give a positive effect on the psychosocial wellbeing when people under the stress by MERS shows adverse effects on psychosocial wellbeing. This suggests that clinical intervention and psychosocial approach aiming at strengthening resilience is important to maintain mental health during crisis development.

Evaluation for detection of Cryptosporidium oocysts in diarrheal feces of calves (야외 송아지 설사변에서 작은와포자충 검출에 대한 평가)

  • Wi, Seong-Hwan;Ju, Hu-Don;Gang, Yeong-Bae
    • Parasites, Hosts and Diseases
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    • v.34 no.2
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    • pp.121-126
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    • 1996
  • For the detection of Cwptospori,mum oocysts, fecal samples were collected from 201 calves which showed diarrhea. Among the 201 samples, 29 samples (14.4%) were positive for Cwptosporinium spry. by the DMSO-modified acid-fast stain (MAFS) , 23 samples (11.4%) were positive by commercial kit (Meridian Diagnostics, Cincinnati, Ohiol and 23 by the indirect immunofluorescence antibody (IFA )assay employing the monoclonal antibody (mAb C6). When tested by both IFA and MAFS, 20 fecal samples were positive for Cwptosporinium oocysts whereas 169 fecal samples were negative. If the MAFS is considered a standard method for oocyst detection, the IFA showed 69% of sensitivity and 98% of specificity. When tested by both IFA and commercial kit, 22 fecal samples were positive for Cwptospori,mum oocysts while 177 samples were negative. One sample tested by IFA was found to be false negative, when compared with the results by commercial kit. The sensitivity of IFA was calculated as high as 96%; the specificity as 99% and the predictive value was also 99%. In the present study, IFA employing the nAb C6 revealed that 23 samples (11.4%) were positive among the 201 calves showing diarrhea. Of 23 IFA positive samples, 4 samples (5%) showed cryptosporidial oocysts more than 105 OPG Therefore. it is concluded that the calves showing cryptosporidial oocysts more than 105 OPG in the feces were highly associated with clinical cryptosporidiosis.

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Development of Organic Fertilizer based on the Cow Dung III. Studies on Tentative Guideline for Degree of Maturity (우분(牛糞)의 유기질비료화(有機質肥料化) 연구 III. 부숙도(腐熟度) 기준설정(基準設定))

  • Lim, Dong-Kyu;Jeong, Lee-Geon;Shin, Jae-Sung;Han, Ki-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.4
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    • pp.278-285
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    • 1991
  • This study was conducted to manufacture a good qualified organic fertilizer with cow dung through rapid composting process, and the proposal guideline of the degree of maturity could be estimated with the measurement of final product. It included total nitrogen content of above 2% on an oven-dry material basis, C/N ratio of below 20, CEC of more than about 60 me/100g, ratio of carbon in reducing sugar to the total carbon of below 35%, and temperature in pile of above $60^{\circ}C$. The total nitrogen content, the C/N ratio, and the Paper Chromatographic method couldn't be the guideline to evalute the maturity of cow dung compost. CEC was increased in increased fermentation and it was high in the high fermented temperature plots which were cow dung+ shredded bark in 1988, cow+dung+wood chips in 1989, and cow dung+rice straws in 1990. The ratio of carbon in reducing sugar to total carbon in 1990 was lower in cow dung+saw dust than cow dung+rice straws that was the highest temperature in pile. Generally cow dung was mixed well with saw dust and thus the total carbon of the product was high. The measurement of the temperature in pile seems to be a indirect guideline of maturity.

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Effects of Low-Level Laser Irradiation on the ALP Activity and Calcified Module Formation of Rat Osteoblastic Cell (저수준레이저(GaAs 반도체)조사가 골모세포의 알칼리성 인산분해효소의 활성과 석회화결절의 형성에 미치는 영향)

  • Kyung-Hun Lee;Ki-Suk Kim
    • Journal of Oral Medicine and Pain
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    • v.21 no.2
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    • pp.279-291
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    • 1996
  • 저수준레에저요법에 대해서는 지난 10여년간 의학계 및 치과계에서 임상적으로 사용하여 좋은 결과가 있다고 많은 보고가 발표되고 있다. 특히 치근의 골결손에 관한 연구에서는 전기요법, 초음파요법, 전자장요법 등 뿐만아니라 저수준레이저를 사용하여 골절부내 Callus형성이 촉진되었음을 보고하고 새로운 치료법의 하나가 될 수 있음을 제안한 바도 있다. 본 연구에서는 칼륨비소를 다이오드로 사용한 저수준레이저조사가 골결손의 치유에 어떠한 영향을 미치는지 확인하고자 골모세포의 알칼리성 인산분해효소의 활성화와 석회화결절의 형성을 평가함으로 골모세포의 기능을 조사코저하였다. 실험은 첫째, 9개군으로 나누어 레이저 조사기간에 따른 알칼리성 인산분해효소의 활성화를 조사하였고, 둘째, 이를 근거로 9일간 계속 매일 1회 1.3 J/cm2의 레이저를 조사한 후 펄스의 종류별 차이를 비교하였으며, 세째,레이저펄스별 석회차 결절의 형성 정도를 광학현미경으로 관찰하여 비교분석하였다. 결과, 7일 계속 레이저를 조사한 경우 다른 군에 비해 서서히 ALP의 활성이 증가하였으나 유의한 차이는 없었으며. 따라서 9일동안 레이저를 계속 조사한 경우에는 전체 에너지량이 5.895 J/cm2 인 펄스13과 15가 뚜렷하게 유의한 증가를 보여주었다. 그러나 석회화결절의 형성은 전체 에너지량이 2.546 J/cm2 인 펄스11에서 가장 많았다. 결론적으로 골형성이나 알칼리성 인산분해효소의 활성을 촉진하는 데에는 적절한 레이저 조사조건이 필요하나, 알칼리성 인산분해효소의 활성을 촉진한 펄스와 석회화결절의 형성을 촉진하는 펄스가 서로 다르게 나타난 것은 골형성을 촉진하는 여러요인 들이 저수준레이저에 자극받았을 가능성이 높음을 보여준다 이러한 결과들로 보아 저수준레이저는 골모세포의 기능을 자극하여 골결손의 치유를 개선하는 데 도움될 것이라 사료된다.다. 각 백서의 양측 창상중 하나는 1,3,5,7일 마다 각 실험의 방법에 따라 레이저를 조사하고 실험동물의 다른 창상은 대조군으로서 사용하였다. 모든 창상의 면적은 실험 1,3,5,7 일째에 일정한 거리에서 사진촬영하여 면적계를 이용, 측정한 후 통계적인 의의를 조사하였다. 본 연구의 결과는 저수준레이저는 특정 조건하에서 S. aureus의 증식을 촉진하였다. 그러나 S. aureus에 감염된 창상을 저수준레이저로 조사시 치유가 촉진되었다. 중앙 조사법고 주변조사법에 의한 창상치유효과는 통계적인 의의가 보이지 않았다. 따라서 결론적으로 S. aureus 에 감염된 창상에 직접 또는 간접적이든 pulse의 종류에 관계없이 조사하는 경우 치유효과가 나타나는 것은 정사주위 조직의 LLLI 자극효과가 염증의 확산을 억제한다고 말할수 있다.4/1$0^{\circ}C$에서는 Shoa-Nan-Tsan과 Lenkwang이 가장 높았으며 백앙벼는 3 온도 조건 모두에서 활성이 낮았다. 발아소요일수와 amylase 활성과는 유의적인 정의 상관관계를 보였다., 다다조, 미국의 건답직파재배 품종 등이었으며 우리 나라 육성종들은 모두 지중에서 신장이 멈추어 제1본엽이 지중에서 추출하였으며, Scm파종심에서 불완전엽이 지면을 뚫고 나오는 품종은 Chinsura Boro뿐이었고 Nato, Labelle, Weld Pally, Italliconaverneco 등도 지면 가까이 까지 신장하였다. 6. 50% 출아일수는 제2절간장을 제외 한 모든 유아 형질의 신장도와 유의한 부의 상관을 보였는데 가장 높은 상관을 보인 것은 중배축장+제1절간장+불완전옆장이었으며, 다음이 불완전엽장이 었다. 7. 출아율은 중배축장+제1절간장+불완전엽장, 중배축장+초엽 장과 모든 파종심에서 높은 정의 상관을 보여 제1본엽의 추출 위치가 높을수록

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Comparative Diagnostic Studies on Serologic and Molecular Biological Tests Against Haemorrhagic Fever with Renal Syndrome (신증후출혈열 환자의 혈청학적 및 분자생물학적 진단 검사법 비교)

  • 우영대;문희주;배형준
    • Biomedical Science Letters
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    • v.6 no.2
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    • pp.141-149
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    • 2000
  • The etiologic agents of haemorrhagic fever with renal syndrome (HFRS) in Korea are Hantaan and Seoul virus in the genus Hantavirus, family Bunyaviridae. Antibody titers of sera from HFRS patients against Hantaan virus were measured by immunofluorescent antibody technique (IFAT), enzyme-linked immunosorbent assay (ELISA), high density composite particle agglutination (HDPA) and plaque reduction neutralization test (PRNI). PRNT and nested reverse transcriptase polymerase chain reaction (nested RT-PCR) was used for serotypic differentiation of Hantaviruses against Hantaan and Seoul virus. Eight doubtful HFRS patients showed higher fluorescent, IgG ELISA, agglutination and neutralizing antibody titer by IFAT, ELISA IgG, HDPA and PRNT, respectively Five out of them showed high IgM antibody titer by IgM capture ELISA against Hantaan virus, remarkably. Fifteen HFRS patients showed higher fluorescent antibody titer by IFAT. In PRNT, 12 out of them showed high neutralizing antibody titer against HTNV, 2 against SEOV and 1 against both viruses. In nested RT-PCR using serotype specific-primer, 3 out of them showed positive against HTNV and 1 against SEOV.

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Quality Changes of Orange Juice as Influenced by Clarification Methods (청징방법에 따른 오렌지주스의 품질특성)

  • Sohn, Kyoung-Suck;Seog, En-Ju;Lee, Jun-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.3
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    • pp.378-382
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    • 2006
  • Clarified orange juice was prepared using different clarification methods including centrifugation, enzyme treatment, ultrafiltration (UF), and combined treatment(CT). Effect of clarification methods as well as other quality parameters were investigated. Clarification was improved with increase in centrifugation speed and by lowering operating temperature. The optimum condition for centrifugation process was $5^{\circ}C$ and 10,000 rpm. UF and CT processes were very effective to produce clarified orange juice. The optimum condition of UF process was $45^{\circ}C$ and 150 kPa considering flux and turbidity CT did not significantly improve the clarification efficiency since most of the clarification was already achieved during UF process. $L^*$-values increased while $a^*$-values decreased significantly after clarification regardless of methods (p<0.05) Considering color, and recovering rate of vitamin C and soluble solids, UF process was superior than the other methods in producing clarified orange juice.

Benefit Evaluation for Traffic Transfer System using Contingent Valuation Method in Tourist Resort (CVM을 이용한 관광지 환승 교통시스템의 편익 추정에 관한 연구)

  • Lee, Byeong-Ju;Seo, Im-Gi;NamGung, Mun
    • Journal of Korean Society of Transportation
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    • v.25 no.4
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    • pp.57-66
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    • 2007
  • According to the rapid economic growth and the escalation of recreational tourism activities due to the change of job and working patterns, currently various traffic problems often occur in tourist areas. As tourist traffic, contrary to general traffic, is not regional but rather from outside the area of interest, it is hard to grasp the traffic situation surrounding the tourist area. Furthermore, since the traffic is concentrated during a limited tourist season, it is difficult to examine and solve the problem. In this study, in order to test the feasibility of introducing a traffic transfer system to reduce congestion in tourist areas, the authors considered the differences in traffic congestion between typical and tourist seasons and designed a willingness to pay model. The willingness to pay amount for a shuttle bus fare is assumed as 1,430 Won and 1,650 Won is assumed for a transfer parking charge. These amounts were slightly above the current bus fare in the study area, but many people thought that the introduction of a traffic transfer system would help to relieve the traffic congestion approaching the tourist area and to develop tourism resources. As the willingness to pay appeared high from the persons used in this study, it was indirectly confirmed that the introduction of a traffic transfer system would be a step in the right direction.

A Study on the Contribution of Exterior Devices to Running Resistance in High-Speed Trains (고속열차 외부장치에 의한 주행저항 기여도 연구)

  • Oh, Hyuck Keun;Kwak, Minho;Kwon, Hyeok-bin;Kim, Sang-soo;Kim, Seogwon
    • Journal of the Korean Society for Railway
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    • v.18 no.4
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    • pp.309-316
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    • 2015
  • The contribution of exterior devices such as bogie fairings and pantographs to running resistance was estimated on the basis of coasting tests at up to 350 km/h with the help of the Korean Next Generation High speed train (HEMU-430X). In order to assess the reduction of air resistance by nose car's bogie fairing, coasting tests were conducted with a removable bogie fairing at various speed ranges. And, the contribution of the pantograph to air resistance was also estimated with coasting tests that include the pantograph's rising and descent modes. The linear regression method was used to examine decelerations from time-velocity data and the equation of resistance to motion is proposed from the deceleration data. From the aerodynamic term of the equation of resistance to motion, the contribution to air resistance by nose car's bogie fairing and pantograph was estimated. The results show that the air resistance was reduced by about 3.8% by the nose car's bogie fairing. And, the 3.9% increase of air resistance by the pantograph (open knee mode) has been found.

The Role of Insulin-like Growth Factor I(IGF-I), and IGF Binding Protein (IGFBP) in Mouse Lung Cancer Cells (마우스 폐암 세포에서 Insulin-Like Growth Factor-I (IGF-I) 및 IGF Binding Protein (IGFBP)의 역할)

  • Cho, Chul-Ho;Kim, Se-Kyu;Kwak, Seung-Min;Chang, Joon;Kim, Sung-Kyu;Chung, Kyung-Young
    • Tuberculosis and Respiratory Diseases
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    • v.50 no.5
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    • pp.549-556
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    • 2001
  • Background : IGF-I is an important mitogen in many types of malignancies. Tumors also express many IGF binding proteins, which modulate IGF action. The propose of this study was to evaluate the effect of IGF-I and IGFBP on cell proliferation in mouse lung cancer cells (3LL). Methods : The cellular proliferation of 3LL with the treatment of growth factors was evaluated using MTT assay. Western ligand blot was performed in order to determine whether 3LL cells secrete IGFBPs and we evaluated the effect of IGFBP on cellular proliferation. Results : The treatment of 3LL cells with IGF-I increased cellular proliferation in a serum free media. Western ligand blot of conditioned medium of 3LL with $^{125}I$-IGF-I demonstrated one single major band with an estimated molecular mass of 24 kDa. This band was identified as IGFBP-4 with immunoblot analysis using antisera. The addition of anti-IGFBP-4 antibody to abrogate the effect of IGFBP-4 resulted in increased cellular proliferation suggesting that IGFBP-4 inhibits cell growth. Conclusion : IGF-I increases cellular proliferation, however the secreted IGFBP-4 has an inhibitory function on cell growth in 3LL. These findings suggest that IGF-I and IGFBP are involved in the cell proliferation.

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Effect of Reflow Number and Surface Finish on the High Speed Shear Properties of Sn-Ag-Cu Lead-free Solder Bump (리플로우 횟수와 표면처리에 따른 Sn-Ag-Cu계 무연 솔더 범프의 고속전단 특성평가)

  • Jang, Im-Nam;Park, Jai-Hyun;Ahn, Yong-Sik
    • Journal of the Microelectronics and Packaging Society
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    • v.16 no.3
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    • pp.11-17
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    • 2009
  • The drop impact reliability comes to be important for evaluation of the life time of mobile electronic products such as cellular phone. The drop impact reliability of solder joint is generally affected by the kinds of pad and reflow number, therefore, the reliability evaluation is needed. Drop impact test proposed by JEDEC has been used as a standard method, however, which requires high cost and long time. The drop impact reliability can be indirectly evaluated by using high speed shear test of solder joints. Solder joints formed on 3 kinds of surface finishes OSP (Organic Solderability Preservation), ENIG (Electroless Nickel Immersion Gold) and ENEPIG (Electroless Nickel Electroless Palladium Immersion Gold) was investigated. The shear strength was analysed with the morphology change of intermetallic compound (IMC) layer according to reflow number. The layer thickness of IMC was increased with the increase of reflow number, which resulted in the decrease of the high speed shear strength and impact energy. The order of the high speed shear strength and impact energy was ENEPIG > ENIG > OSP after the 1st reflow, and ENEPIG > OSP > ENIG after 8th reflow.

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