• Title/Summary/Keyword: 가용성 당 조성

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Production of Nitric Oxide in Raw 264.7 Macrophages treated with Ganoderan, the ${\beta}-Glucan$ of Ganoderma lucidum (영지의 균사체성 ${\beta}-glucan$에 의한 Raw 264.7 대식세포의 Nitric Oxide생성)

  • Han, Man-Deuk;Lee, Eun-Sook;Kim, Young-Kweon;Lee, June-Woo;Jeong, Hoon;Yoon, Kyung-Ha
    • The Korean Journal of Mycology
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    • v.26 no.2 s.85
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    • pp.246-255
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    • 1998
  • Ganoderan (GAN), an immunomodulating ${\beta}-glucan$ of G. lucidum, induces potent antitumor immunity in tumor-bearing mice. This study was set up to elucidate the ability of macrophage activation of GANs. GAN-treated Raw 264.7 macrophages showed enhanced production of nitric oxide (NO). The ability of GANs to produce NO was based on differences in chemical composition of GANs obtained from the mycelium on various carbon sources and mycelial fractionation. The highest NO production was observed in CW-AS-WS polysaccharide which was extracted from the mycelial wall. GAN-treated Raw 264.7 cells gave a 2-to 5-fold (24 hr) formation of NO levels compared with those treated with medium only. Partial removal of the protein in the extracellular GAN by TCA treatment did appreciably reduce its capacity to secrete NO. The mixture effect of GAN and LPS increased the nitric oxide secretion from RAW 264.7. The cell proliferation of GAN-treated Raw 264.7 cell tines inhibited as compared with its control. Of the culture supernatant of macrophage activated by GAN, the percentage of cytotoxicity against mouse leukemia L1210 cells was slightly dependent on the amount of NO in the culture supernatants of the activated-macrophages. These results indicate that the ${\beta}-glucan-related$ polysaccharides of the higher fungus activate macrophage and release nitric oxide. It also suggests that murine macrophages possess certain receptors for ${\beta}-anomeric$ glucans and play a critical role of ${\beta}-glucan-related$ tumor killing mechanism.

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Effect of collection time on the chemical composition and levels of thiobarbituric acid reactive substance of Godulbaegi (Youngia sonchifolia M.) (채취시기에 따른 고들빼기의 성분 조성과 산화방지활성)

  • Hwang, Tae Yean;Huh, Chang Ki
    • Food Science and Preservation
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    • v.24 no.6
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    • pp.786-794
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    • 2017
  • This study analyzes the chemical composition and thiobarbituric acid reactive substance levels of Godulbaegi (Youngia sonchifolia M.) depending on collection time. The moisture and crude fat content in leaf and root decreased, while crude fiber, crude protein, carbohydrate, and ash increased with increases in collection time. The mineral elements tended to increase in each sample with increases in collection time. The content of vitamin B increased as collection time increased. Vitamin C content was approximately five times higher in the leaves than that in the roots. Total amino acids in leaf and root increased considerably as collection time increased content of phenolic compounds in root were higher than that in the leaf and these contents increased. Antioxidant activity of Godulbaegi was higher in the root than in the leaf and increased as collection time increased.

Food Component Characterization of Muscle From Salmon Frame (연어 Frame 육의 식품성분 특성)

  • Heu, Min-Soo;Kim, Hyung-Jun;Yoon, Min-Seok;Park, Do-Yeong;Park, Kwon-Hyun;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.11
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    • pp.1452-1456
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    • 2008
  • For the effective use of salmon processing by-products, the food components of salmon frame muscle were investigated and compared with those of fillet muscle. The proximate composition of salmon frame muscle was 73.2 g/100 g muscle for the moisture, 76.9 g/100 g dry material for the protein, 15.7 g/100 g dry material for the lipid and 4.1 g/100 g dry material for the ash. pH and volatile basic nitrogen (VBN) content of salmon frame muscle were 6.63 and 16 mg/100 g, respectively. The proximate composition, pH and VBN of salmon frame muscles were similar to those of salmon fillet muscle. The Hunter values of salmon frame muscle were 55.34 for L value, 16.60 for a value, 19.99 for b value and 48.83 for ${\Delta}E$ value, which were different compared to the salmon fillet muscle. The trichloroacetic acid (TCA) soluble-N content of salmon frame muscle was 542 mg/100 g, which was lower than that of salmon fillet muscle. No difference was found in fatty acid composition, total amino acid, calcium, phosphorus contents and sensory evaluation between salmon frame muscle and salmon fillet muscle. These results suggested that muscle from salmon frame could be used as resources for seafood processing.

Effects of Organic Liquid Fertilizer on Growth and Fruit Quality of Hot Pepper (유기액비 시용량이 고추의 생육 및 품질에 미치는 영향)

  • Joo, Seon-Jong;Jeon, Jong-Ok;Lee, Guang-Jae
    • Korean Journal of Organic Agriculture
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    • v.18 no.2
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    • pp.209-219
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    • 2010
  • This study was carried out to evaluate the effects of applying levels of organic liquid fertilizer (OLF) on growth and fruit quality of hot pepper. The OLF was made with compositions of chicken dung : rice bran : soybean meal (2:1:1). Hot pepper was applied with 5 treatments; control (chemical fertilize), OLF-1.0X (standard applying levels), OLF-0.7X (70% of standard applying level), and OLF-1.3X (130% of standard applying level). The plant height was reduced at 23cm in the OLF-1.0X treatment compared to control of 293cm. The number of hot pepper fruits was significantly increased. The content of capsaicin in the ripened pepper decreased by 23.6% in the OLF-1.0X treatment with $253mg{\cdot}kg^{-1}$ compared with control of $331mg{\cdot}kg^{-1}$, but the content of free sugar increased by 19.5%. The content of capsaicin and soluble solid content in OLF-1.3X treatment were significant different with other treatments. The yield of red pepper in OLF-1.0X treatments increased by 15% with $4,190kg{\cdot}10a^{-1}$ compared to control of $3,643kg{\cdot}10a^{-1}$, and OLF-1.3X and OLF-0.7X treatments showed increase by 10% and 6%, respectively.

Chemical Components of White and Red Lotus (목련과 홍련의 화학성분)

  • Jeong, Chang-Ho;Son, Ki-Bong;Kang, Seon-Gyeong;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.43 no.1
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    • pp.43-50
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    • 2009
  • To get basic data for the utilization of white and red lotus as a raw material in functional food, chemical components of its leaf and root were investigated. Leaf had the highest level of nitrogen free extract and root had the highest level of moisture. The mineral analysis showed K(380.44~1,516.34 mg/100g), Ca(36.67~1,323.92 mg/100g), P(84.02~473.13 mg/100g) and Na(57.73~304.07 mg/100g). The rich free sugars in white and red lotus were glucose(161.12~765.15 mg/100g) and frutose (100.57~901.12 mg/100g). Total amino acid contents in leaf and root of white lotus and leaf and root of red lotus were 6,385.57, 1,162.93, 6,003.01 and 1,242.20 mg/100g, respectively. Although the free amino acid compositions of white and red lotus were different, their major free amino acids were glutamic acid, phenylalanine arginine and tyrosine. The ascorbic acid were 248.65 and 20.99 mg/100g in leaf and root of white lotus, 156.92 and 9.32 mg/100g in leaf and root of red lotus. The leaf of white lotus exhibited the highest total phenolic contents at 24.33 mg/g.

Studies on the Chemical Compositions of Korean Sweet Potatoes ('한국산(韓國産) 고구마의 화학적(化學的) 조성(組成)에 관(關)한 연구(硏究)')

  • Kim, Ho-Sik;Lee, Chun-Yeong;Kim, Jae-Uk
    • Applied Biological Chemistry
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    • v.4
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    • pp.1-9
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    • 1963
  • For the studies on the Chemical compositions of Sweet potatoes grown in Korean soil, Suwon No. 118 and Suwon No. 147 were applied as the samples during growing period, and 15 varieties of swee tpotatoes as the samples or comparisons among them. As the results of the studies followings were obtained. Changes of the chemical compositions of root tuber during growing period. 1. Total weights of root tubers and mean weight per root tuber were increased gradually as grew with the values of Suwon No. 147 is higher than that of Suwon No. 18 except the weight of Suwon No. 147 on September 9. 2. The moisture content of the roots were fairy uniform. 3. While the starch contents and crude starch yield in the root tuber were gradually increased with almost parallel as grew except the values of Suwon No. 147 on September 7. were markedly higher. 4. The total weight of the Sweet potatoes per Dan-Bo (about 0.25 acre) showed increased values with Suwon No. 147 is higher than Suwon No. 118 except the unexpected lower weight of Suwon No. 147 on September 9. and the crude starch yield of Suwon No. 147 per Dan-Bo also showed higher values than that of Suwon No. 18 with almost parallel increase of them as they grew. 5. Reducing sugar contents of them showed gradual decreases at earlier stages then increases at latter stages as grew, and total sugar and sucrose of them also showed gradual increases except extremly higher contents of Suwon No. 147 and lower values of Suwon No. 118 on September 9. 6. Total protein and soluble protein contents of them showed that initial and last stages of the growth are in higher values but middle stages are fairy low values with a little changes of difference between total protein, and soluble protein. The comparisons among those varieties. 7. The moisture contents of root tuber varies from 63% to 72% among them. 8. The starch contents of Suwon No. 18 (23.9%) is highest value among them, Ko-Ke No. 14 and Won-Ki successively lower and Dae-Nong No. 45 is the lowest one. Crude starch yield (%) of Ko-Ke No. 14 and Won-Ki successively lower and Dae-Nong No.45 is the lowest one. Crude starch yield (%) of Ko-Ke No. 147 is highest value, Suwon No. 118, Won-Ki are successively lower and Do-Ip is the lowest one. 9. Won-Ki is highest value in reducing sugar content, and Do-Ip No. 2 is lowest one in it. The sucrose content of Chil-Bok is highest and Won-Ki is lowest among them. Soluble total sugar content of Yu-Sim is in highest and Chun-Mi is in lowest value. 10. Total protein content showed that Suwon No. 147 is in highest value and Yu-Sim is in lowest one. On the other hand, soluble protein contents showed that Chil-Bok is in highest value and Yu-Sim is in lowest one.

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Effect of Different Extraction Procedures on Chemical Composition of Ginseng Extract (추출조건이 인삼엑기스의 화학성분 조성에 미치는 영향)

  • U, In-Hui;Yang, Cha-Beom;Seong, Hyeon-Sun
    • Journal of Ginseng Research
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    • v.10 no.1
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    • pp.36-44
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    • 1986
  • Fresh ginseng roots were extracted by different extraction methods to estimate the amount of extracts, and the content of common constituents and ginsenosides for comparison. The results are as follows: 1 The amount of the extract obtained by water as a solvent was about five times higher than those by ethanol or pressing process. 2. Water extraction at 70-$80^{\circ}C$ gave highest value in saponin yield, which was reduced by half by boiling, 3. The saponin yield by pressing process was shown to be about 52% of total saponin; saponins belonging to protopanaxatriol-ginsenosides being extracted better than those belonging to proto-panaxadiol-ginsenosides . 4. The contents of total sugar, reducing sugar, crude protein and total amino acids in the water extract were revealed to be higher compared to those in an ethanol extract. 5. The extract obtained by press had the highest ash content.

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Physicochemical Characteristics of Rubus coreanus Miquel (복분자 딸기의 이화학적 특성)

  • 차환수;이문경;황진봉;박민선;박기문
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.6
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    • pp.1021-1025
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    • 2001
  • Physicochemical properties of Rubus coreanus Miquel were examined. Unripened fruit, ripened fruit and leaf of Rubus coreanus Miquel were used as samples. The contents of water, crude protein, crude fat, crude fiber, dietary fiber, and crude ash were studied. The pH, soluble solid content, acidity and color were also measured. In terms of free sugar composition, concentration of glucose and fructose found in unripened fruit had increased in ripened fruit and sucrose was contained in ripened fruit. The major free sugars of leaf were glucose, fructose and sucrose. Citric acid, succinic acid and fumaric acid were found in all three sample groups, unripened fruit, ripened fruit and leaf. The citric acid showed the highest concentration in the unripened fruit as the concentration of 1.21%. Fifteen types of amino acids were analysed from the unripened and ripened fruit of Rubus coreanus Miquel. The leaf contained methionine in addition to those fifteen type amino acids in fruits. The glutamic acid contents of unripened fruit, ripened fruit and leaf were 411, 246.59 and 898.42 mg%, respectively. Among the minerals studied in this research Rubus coreanus Miquel held high level of potassium. The potassium concentrations of unripened fruit, ripened fruit and leaf were 645.07, 216.53 and 815.78 mg%, respectively.

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The antioxidant capacities of imported red wines (Cabernet Sauvignon) from US and Chile (미국 및 칠레산 수입 레드 와인(Cabernet Sauvignon)의 항산화능)

  • Lee, Hye-Ryun;Hwang, In-Wook;Ha, Hyoung-Tae;Chung, Shin-Kyo
    • Food Science and Preservation
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    • v.20 no.5
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    • pp.608-613
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    • 2013
  • The physicochemical characteristics and antioxidant capacities of 15 red wines (Cabernet Sauvignon) from the US (5) and Chile (10) were investigated. The contents of soluble solid, reducing sugar, titratable acidity, and $SO_2$ were measured. Antioxidant capacities were examined by DPPH, ORAC assay, and total phenolic contents. In addition, polyphenols composition were analyzed by HPLC. The contents of soluble solid, reducing sugar, and acidities were 7.03~8.6 $^{\circ}Brix$, 2.7~6.7 g/L, and 0.7~0.8%, respectively, and showed no differences between wines from the two countries (p<0.05). The $SO_2$ content of Chile wines was 50% higher than that of US wines (p<0.05). Antioxidant activities by DPPH assay ranged 5.58~9.80 mM and 6.77~9.48 mM in the US and Chile wines, respectively. The ORAC values of the US and Chile wines ranged 2.17~18.08 mM and 4.55~33.77 mM, respectively. The total phenolic content ranged from 1,315 to 2,651 mg/L among the US red wines, and from 1,653 to 2,493 mg/L among Chile red wines. Gallic acid, catechin, syringic acid, p-coumaric acid, quercetin, and kaempferol were identified by HPLC. The polyphenol contents of the Chile red wines were higher than those of the US red wines(p<0.05). There were no differences in the physicochemical characteristics and the antioxidant capacities of the wines from US and Chile, but only in their $SO_2$ and polyphenol contents (p<0.05).

Comparison of Characteristics on Electrolyzed Water Manufactured by Various Electrolytic Factors (전해인자에 따른 전기분해수의 특성 비교)

  • Kim, Myung-Ho;Jeong, Jin-Woong;Cho, Young-Je
    • Korean Journal of Food Science and Technology
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    • v.36 no.3
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    • pp.416-422
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    • 2004
  • Efficacy of surface sterilization and physicochemical properties of electrolyzed water manufactured depending on electrolyte, materials, and type of electrolytic diaphragm used were investigated. Physical properties of electrolyzed water manufactured from diaphragm system showed the highest effectiveness under at distance between diaphragms of 1.0 mm and 20% NaCl supplying rate of 6 mL/min. ORP, HClO (should defined) content, and pH at above conditions were 1,170 mV, 100 ppm, and 2.5, respectively. Two-stage electrolyzed system was more effective than one-stage one. Electrolyzed water manufactured from non-diaphragm system at 4 mL/min supplying rate of 20% NaCl was similar to the most effective diaphragm system, whereas ORP, HClO content, and pH were 800 mV, 200 ppm, and 9, respectively. Sealed electrolyzed water could be preserved more than one month at room temperature with ORPs of 750 and 1,150 mV in non-diaphragm and diaphragm systems, respectively, and at HClO content of 100 ppm. Physicochemical properties of electrolyzed water manufactured from electrolytic diaphragm of $IrO_{2}$ and Pt+Ir were more effective than that of Pt. ORP and HClO contents of electrolyzed water manufactured from various electrolytes were high in order of NaCl>KCl>$CaCl_{2}$, whereas no differences were observed among electrolytes in sterilization efficacy. Twelve kinds of microorganisms tested (initial total count, $10^{5}-10^{6}CFU/mL$) were sterilized within 1-2 min by electrolyzed water.