• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.347-358
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• 2016

Brassinosteroid (BR), a plant steroid hormone, plays key roles in numerous growth and developmental processes as well as tolerance to both abiotic and biotic stress. To understand the biological networks involved in BR-mediated signaling pathways and stress tolerance, we performed comparative genome-wide transcriptome analysis of a constitutively activated BR bes1-D mutant with an Agilent Arabidopsis $4{\times}44K$ oligo chip. As a result, we newly identified 1,091 (562 up-regulated and 529 down-regulated) significant differentially expressed genes (DEGs). The combination of GO enrichment and protein network analysis revealed that stress-related processes, such as metabolism, development, abiotic/biotic stress, immunity, and defense, were critically linked to BR signaling pathways. Among the identified gene sets, we confirmed more than a 6-fold up-regulation of NB-ARC and FLS2 in bes1-D plants. However, some genes, including TIR1, TSA1 and OCP3, were down-regulated. Consistently, BR-activated plants showed higher tolerance to drought stress and pathogen infection compared to wild-type controls. In this study, we newly developed a useful, comprehensive method for large-scale identification of critical network and gene sets with global transcriptome analysis using a microarray. This study also showed that gain of function in the bes1-D gene can regulate the adaptive response of plants to various stressful conditions.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.9-22
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• 2009

Due to increasing concerns over issues with both water quantity and quality for turfgrass use, research was conducted to determine the response of five warm-season turfgrasses to deficit irrigation and to gain a better understanding of relative drought tolerance. St. Augustinegrass(Stenotaphrum secundatum [Walt.] Kuntze.) cultivars 'Floratam' and 'Palmetto', 'SeaIsle 1' seashore Paspalum(Paspalum vaginatumSwartz.), 'Empire' zoysiagrass(Zoysia japonica Steud.), and 'Pensacola' bahiagrass(Paspalum notatum Flugge) were established in lysimeters in the University of Florida Envirotron greenhouse facility in Gainesville. Irrigation was applied at100%, 80%, 60%, or 40% of evapotranspiration(ET). Evaluations included: a) shoot quality, leaf rolling, leaf firing; b) leaf relative water content(RWC), soil moisture content, chlorophyll content index(CCI), canopy photosynthesis(PS); c) multispectral reflectance(MSR); d) root distribution; and e) water use efficiency. Grasses irrigated at 100% and 80% of ET had no differences in visual quality, leaf rolling, leaf firing, RWC, CCI, and PS. Grasses irrigated at 60% of ET had higher values in physiological aspects than grasses irrigated at 40% of ET. 'Sealsle 1' and 'Palmetto' had a deeper root system than 'Empire' and 'Pensacola', while 'Floratam' had the least amount of root mass. Photosynthesis was positively correlated with visual assessments such as turf quality, leaf rolling, leaf firing, and sensor-based measurements such as CCI, soil moisture, and MSR. Reducing the amount of applied water by 20% did not reduce turfgrass quality and maintained acceptable physiological functioning.

• Journal of Environmental Policy
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• v.7 no.4
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• pp.35-55
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• 2008

Assessing vulnerability to climate change is the first step to take when setting up appropriate adaptation strategies. Adaptive capacity to climate change is the important factor comprising vulnerability. An adaptive capacity index in agricultural water management system was developed considering agricultural water supply and demand for rice production in Jeolla-do, Korea. The agricultural water supply was assumed to be equal to the amount of water stored in the major agricultural reservoirs, while data on the agricultural water demand was obtained from the dynamic simulation results by Korea Agriculture Corporation(KAC). The spatial unit for analysis was conducted at the county(Si, Gun, Gu) level and temporal scale was based on every month from 1991-2003. Adaptive capacity for drought stress index(ACDS index) was calculated as the percentage of data points where the irrigated water supply was greater than the crop water demand. The ACDS index was compared with SWSCI(Standard Water Storage Capacity Index) and the relationship showed high degree of fit($R^2$=0.84) using the exponential function, indicating that the developed ACDS index is useful for evaluating the status of the balance between agricultural water supply and demand, especially for the small sized agricultural reservoirs. This study provided the methodological basis for developing climate change vulnerability index in agricultural water system which is projected to be more frequently exposed to drought condition in the future due to climate change. Further research should be extended to the study on the water demand of the crops other than rice and to the projection of the change in ACDS index in the future.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.41-41
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• 2017

본 연구는 초분광 영상을 이용하여 수박 모종의 수분함량을 비파괴적으로 추정하기 위해 수행되었다. 단계적으로 수분 스트레스를 받은 수박(n=45) 모종을 초분광 영상시스템으로 촬영하여 모종 영역의 반사율을 추출하였고, 매 촬영 후 모종의 생체중과 건물중을 측정하여 수분함량을 계산하였다. 모종의 반사율과 계측된 수분함량을 변수로 하여 Partial Least Square Regression(PLSR) 분석을 이용하여 수분 추정 모델을 구축하였다. 수분 추정모델을 작성한 결과 Calibration(Cal.)의 정확도($R^2$)는 0.66, 정밀도(RMSE 및 RE)는 각각 1.06%, 1.14%로 나타났다. 수박 모종의 수분함량 추정모델의 정밀도는 상당히 높게 나타났으나 정확도는 낮게 나타났다. 정확도를 개선하기 위해 Confidence ellipses의 신뢰구간을 95%로 설정하였을 때 3개의 모종이 타원 밖에 위치하는 것을 발견하였으며 이를 제거 후 재분석을 하였다. 3개의 모종을 제외한 수박 모종의 수분함량 추정모델의 정확도는 0.82, 정밀도는 0.73%, 0.78%로 나타났다. 3개의 모종을 제외함으로서 모델의 정확도 및 정밀도가 상승하여 3개의 모종이 정확도 및 정밀도를 낮추는 원인이라 판단된다. 작물은 가뭄스트레스를 받을수록 반사율이 낮아지지만(Yang et al., 2010) 3개의 모종은 다른 모종의 수분함량에 비해 반사율이 큰 차이를 나타내어 정확도 및 정밀도를 낮춘 것으로 판단된다. 본 연구를 통해 초분광 영상을 이용하여 수박 모종의 수분함량 추정가능성을 시사하였고, 모델의 정확도를 개선하기 위해 샘플 수 및 수분함량의 변이를 증가시키는 것이 필요하다고 판단된다.

• Journal of Life Science
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• v.30 no.5
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• pp.411-419
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• 2020

The development of GM crops has gained significant economic importance, and the number of countries cultivating commercial GM crops has continuously increased since the 1960s. Globally, the area given to cultivating GM soybean, maize, cotton, and canola alone had reached 114 million hectares by 2007. Although the economic importance of cultivating and commercializing GM crops has increased, there is still a need to assess their agricultural traits in comparison to non-GM produce. This study evaluated the agricultural traits of GM rice containing the drought-tolerant gene CaMsrB2 and standard rice to investigate any unintended effects of genetic engineering. The GM and non-GM rice were compared in terms of various agricultural traits in a drought greenhouse and an irrigated paddy field. There was no statistical difference in the field-grown crops, but there was a statistically significant difference in both tiller number and yield in the greenhouse. These results therefore suggest that GM rice lines containing the CaMsrB2 gene are superior in performance to non-GM rice in drought stress conditions and could be grown in drought-prone areas where drought intolerant rice may not be able to grow.

• Journal of Korea Water Resources Association
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• v.55 no.3
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• pp.205-215
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• 2022

Amidst the global climate crisis, dam operation policies formulated under the stationary climate assumption could lead to unsatisfactory water management. In this work, we assessed status-quo performance of the Yongdam Dam in Korea under various climatic stresses in flood risk reduction and water supply reliability for 2021-2040. To this end, we employed a decision-centric framework equipped with a stochastic weather generator, a conceptual streamflow model, and a machine-learning reservoir operation rule. By imposing 294 climate perturbations to dam release simulations, we found that the current operation rule of the Yongdam dam could redundantly secure water storage, while inefficiently enhancing the supply reliability. On the other hand, flood risks were likely to increase substantially due to rising mean and variability of daily precipitation. Here, we argue that the current operation rules of the Yongdam Dam seem to be overly focused on securing water storage, and thus need to be adjusted to efficiently improve supply reliability and reduce flood risks in downstream areas.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.69-69
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• 2020

식물체가 건조 스트레스를 받으면 각 기관 물질 생산의 변이, 분화 및 발달 억제를 통해서 식물의 생산활동을 현저히 저하시켜, 식물의 생장, 형태, 개체발생 및 대사생리에 영향을 미치는 것으로 연구되어 왔다. 최근 기후 온난화로 인해 온도가 점진적으로 상승하고 가뭄과 같은 이상기상이 빈번하게 발생함에 따라 많은 노지 작물의 농업 생산성이 약화되고 있다. 배추는 우리나라의 대표적인 식품 중 하나인 김치의 주재료로 연중 안정적인 생산과 공급이 필요시 되지만, 배추의 경우 건조 조건에서 엽육조직의 붕괴와 같은 생리장해가 발생되기 때문에 최근 발생되고 있는 이상 기후의 영향으로 안정적인 생산이 어려워지고 있는 실정이다. 하지만, 배추에서는 이상 기후에 대응이 가능한 내건성 품종의 육성을 위한 연구는 미흡한 실정이다. 따라서 본 연구에서는 내건성이 높은 배추 개발을 위한 기초소재를 찾는 것을 목적으로 농촌진흥청 유전자원센터에서 보존하고 있는 재래종 배추 30계통을 분양받아 강릉원주대학교 생명과학대학 내의 조직배양실을 이용하여 연구를 수행하였다. 배지는 Tissue Culture Square Dish(125×125×20mm)에 Agar를 녹여 40ml씩 분주하여 고체배지를 조성한 후, 건조 처리구 Polyrthylene glycol 6000(PEG) 0%(Control), 20%(Mild Stress), 30%(Severe Stress)를 설정하여 60ml씩 추가 분주하여 배양기 28℃에서 15시간 처리를 하였다. 분양받은 각 계통의 종자는 1% 차아염소산 나트륨으로 10분간 종자표면을 살균한 후, 5번 정도 멸균수로 헹군 후, 표면 살균한 재래종 종자를 고체배지 시험관에 6립씩 치상하였다. 식물체 생육은 각 처리구별 3반복으로 하였으며 주간 12시간 주기, 광도 2,400Lux, 온도 20℃의 조직배양실에서 치상 후 7일간 생육하였다. 치상 후 7일간 생육시킨 식물체를 채취하여 지상부생체중, 지하부 생체중, 뿌리 길이를 측정하였으며, 지상부와 지하부로 나누어 50℃에서 72시간 건조시킨 후, 건물 생산량을 조사하였다. 본 실험 결과 건조 처리(PEG-6000)는 배추의 생장을 저해하였지만, 생육 저해 정도는 계통간의 차이가 있는 것을 관찰할 수 있었으며 무처리구와 스트레스 처리구간의 생장량 변화 정도를 기반으로 군집분석을 수행한 결과 'IT110483'과 'IT104903' 계통이 실험에 공시된 계통들 중 상대적으로 강한 건조 내성을 가진 것을 확인할 수 있었다. 따라서, 재래종 배추 계통 중 일부는 건조내성이 강한 새로운 배추 품종을 육성하는데 있어 유용하게 이용될 수 있을 것으로 사료된다.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.361-368
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• 2009

Cenangium ferruginosum was known as the causal agent of dieback of pines including Pinus koraiensis and Pinus densiflora. Since the first report of the disease in Korea in 1989, a group dying occurred in Seoul, Gyeonggi, Kangwon and Chungbuk in 2007 spring. Although C. ferreginosum was known as a weak pathogen or a parasite, this disease caused in stressed pine by drought, wounding, extremely cold weather or unusual warm winter. In this study, we explained the features of cenangium dieback with the characteristics of pathogen to understand the trend of disease associated with the climatic change of the world. We collected pycnidia and apothecia from the diseased branches and stems of P. koraiensis and P. densiflora in Gyeonggi, Chungcheong and Gyeongsang province to characterization of pathogen. The fungal development on the diseased branches were observed and the isolates from pycnidia and apothecia were identified as Cenangium ferruginosum by their morphological characteristics and the molecular techniques.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.3
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• pp.47-57
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• 2021

Drought monitoring over paddy field area is an important role as the frequency and intensity of drought due to climate change increases. This study analyzed the applicability of drought monitoring on paddy crops using MODIS-based field surveys. As a satellite-based drought index using evapotranspiration for quantitative drought determination, ESI (Evaporative Stress Index), was applied and calculated through the ratio of MODIS- based actual and potential evapotranspiration. For the irrigated areas of Idong, Gosam, Geumgwang, and Madun reservoirs the availability of irrigation water supply, ponding depth, precipitation, paddy growth were investigated for the paddy field within one grid of MODIS. In addition, the percentile-based ESI drought severity was calculated to compare the growth process of paddy and changes in the drought category of ESI. The Idong area was irrigated about a week later than other reservoirs for the period of water supply, transplanting, and water drainage and the ESI drought category tended to be different. The Gosam, Geumgwang, and Madun area expressed moderate drought prior to the farming season, and indicated normal as the water was supplied. During the water drainage, the drought category intensified, indicating that the water available on land was decreasing. These results demonstrated that the MODIS-based ESI could be an effective tool for agricultural drought monitoring over paddy field area.

• Journal of Appropriate Technology
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• v.5 no.2
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• pp.118-125
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• 2019

Climate change is one part of 17 Sustainable Development Goals (SDGs). According to the Fifth Assessment Report by the Inter- governmental Panel on Climate Change(IPCC) published in 2014, global warming is caused by greenhouse gas (GHG) emissions. The most important GHG is carbon dioxide (CO₂), which is released by the burning of fossil fuels and, to a lesser extent, by land use practices, followed by nitrous oxide and methane. IPCC predicts that global temperatures will rise 3.7℃ and sea level will rise 0.63 m by 2099 in the case of no strong restraint. According to the report, we can expect a massive species extinctions, changes in storm and drought cycles, altered ocean circulation, and redistribution of vegetation by global warming. However, climate changes, especially global warming, are the largest potential threat to human health and the source of a number of diseases globally. If climate changes are continued uncontrolled, human health will be adversely affected by the accelerating climate change and the natural disaster induced by climate change. It means we will face more serious conditions of injury, disease, and death related to natural disasters such as flood, drought, heat waves, malnutrition, more allergy, air pollution and climate change related infections related to morbidity and mortality. This review emphasizes on the relationship between global climate changes and human health and provides some suggestions for improvement.