• Clinical and Experimental Reproductive Medicine
• /
• v.26 no.3
• /
• pp.467-474
• /
• 1999

Objectives: Cytogenetic investigations were carried out on 770 women with primary (n=560) and secondary amenorrhea (n=210) to determine the frequency of chromosomal or genetic causes of amenorrhea. Materials and Methods: In 770 women with primary amenorrhea (n=560) and secondary amenorrhea (n=210), chromosomal analysis were performed. Results: 1) The most prevalent age group is 16-20 years of age group with primary amenorrhea and 26-30 years of age group with secondary amenorrhea. 2) Out of 560 cases of primary amenorrhea, 343 cases (61.3%) had the normal chromosome constitution and 217 cases (38.7%) had the abnormal chromosome constitution including 46,XY. 3) In 217 cases of abnormal chromosome of primary amenorrhea, 57 cases (26.3%) had 45,X and 34 cases (15.8%) had the 46,XY, 24 cases (11.0%) had 45,X/46,X,i (Xq), 23 cases (10.6%) had 45,X/46,X,+mar and 14 cases (6.6%) had 45,X/46,XY. 4) Out of 210 cases of secondary amenorrhea, 181 cases (86.2%) had the normal chromosome constitution and 29 cases (13.8%) had the abnormal chromosome. 5) In 29 cases of abnormal chromosome of secondary amenorrhea, 7 cases (24.1%) had 45,X/46, X,i (Xq), 4 cases (13.8%) had 45,X/46,XX. Conclusion: High percentage of chromosomal abnormalities was diagnosed in primary amenorrhea and most of them were sex chromosome anomalies. In secondary amenorrhea, the prevalence was lower than primary amenorrhea, so a preselection of patients with secondary amenorrhea for cytogenetic investigations seems to be necessary.

• Clinical and Experimental Reproductive Medicine
• /
• v.26 no.1
• /
• pp.67-73
• /
• 1999

The insulin-like growth factor (IGF)s are believed to one of several growth factors that play an adjunctive role in ovarian follicular development. These factors circulate bound to a family of IGF-binding protein (IGFBP)s. It is known that circulating IGFBPs are involved in the transport of IGFs to tissues and modulate IGFs actions at local tissue. The purposes of this study were to evaluate changes in serum IGFBPs profiles during normal ovulatory menstrual cylce and to compare serum IGFBPs profiles in periovulatory phase of between normal ovulatory menstrual cylce and controlled hyperstimulated cycle. Fasting blood samples were obtained from 15 normal healthy women throughout normal ovulatory menstural cyle and on the day of aspiration of oocyte from 10 patients undergoing ovarian hyperstimuation for in vito fertilization-embryo transfer. Serum IGFBP-1 - IGFBP-4 were measured by western ligand blot and immunoprecipitation. Serum $17{\beta}$-estradiol was determined by radioimmunoassay. Type and molecular weight of serum IGFBP did not changed during normal ovulatory menstural cycle. No significant variation in the relative proportion and level of each IGFBP was found throughout normal ovulatory menstural cyle. Also, the relative proportion and level of each IGFBP did not correlated with serum $17{\beta}$-estradiol level. There was no significant difference in the relative proportion and level of each serum IGFBP between on the day of ovulation in normal ovulatory menstrual cylce and on the day of aspiration of oocyte in controlled hyperstimulated cycle. Our data indicate that IGFBPs have regulatory functions in ovary through an paracrine and autocrine rather than endocrine mechanism during normal ovulatory menstural cycle.

• Clinical and Experimental Reproductive Medicine
• /
• v.13 no.2
• /
• pp.101-112
• /
• 1986

It is now common practice to attempt ovarian hyperstimulation in vitro fertilization and embryo transfer (IVF-ET) to promote the development of multiple preovulatory follicles and to maximize the number of mature egg available. There are several drugs for hyperstimulation such as clomiphene citrate only, clomiphene citrate and human menopausal gonadotropin (HMG) and HMG only. Accumlated experience has shown that the hyperstimulation of the ovary in IVF-ET results in high pregnancy rate. But the hyperstimulation of the ovary in IVF-ET may cause the hyperandrogenism, so we must consider the adverse effect on pregnancy rate of the hyperandrogenism. Little is known about the functional significance of androgen for the follicular growth, however, the hyperandrogenism might interfere with oocyte maturation. The aim of the present investigation was to determine the serum profiles of estradiol, androstenedione and testosterone during the hyperstimulated menstrual cycles in IVF. The results were summarized as follows: 1. There was a gradual increase in the mean levels of serum estradiol, androstenedione, and testosterone approaching follicular maturation. 2. The mean serum estradiol levels in the hyperstimulated groups were significantly higher than that in the control group in late follicular phase and ovum retrieval (ovulation) day (p<0.01). 3. The mean serum androstenedione levels in the clomiphene citrate groups were significantly higher than that in the control group in late follicular phase (p<0.01). There was no statistically significant different in the mean serum androstenedione levels between the control group and the HMG group (p>0.05). 4. There was no statistically significant difference in the mean levels of testosterone among each group (p>0.05). 5. There was no statistically significant different in the mean levels of estradiol, androstenedione and testosterone between the fertilized patients and non-fertilized patients in clomiphene citrate and HMG group (p>0.05).

• Clinical and Experimental Reproductive Medicine
• /
• v.13 no.2
• /
• pp.153-159
• /
• 1986

The high rate of chromosomal abnormalities in patients with primary amenorrhea implies the need for routine screening for chromosomal abnormalities among such patients. This study was designed for the cytogenetic analysis of 236 patients with primary amenorrhea, which was referred to Yonsei University Medical Center, from January, 1, 1974 to December, 31, 1985. The results were as follows: 1. Of the 236 patients, 145 cases (61.4%) showed normal karyotype, and 91 cases (38.6%) showed chromosomal abnormalities. 2. Gonadal dysgenesis was found in 56 cases, consisting of 42 cases, Turner's syndrome, 12 cases, pure gonadal dysgenesis, and 2 cases mixed gonadal dysgenesis. a) Turner's syndrome was found in 42 cases, consisting of 18 cases of 45, X and 24 cases of mosaicism. b) Pure gonadal dysgenesis was found in 12 cases, consisting of 10 cases of 46, XX and 2 cases of 46, XY. c) Mixed gonadal dysgenesis was found in 2 cases, consisting of 1 case of 46, XY and 1 case of 45, X/46, XY. 3. Intersex was found in 80 cases, consisting of 35 cases of 46, XX, and 45 cases of 46, XY. 4. Congenital anomalies of reproductive system was found in 82 cases and all cases were normal karyotype.

• Korean Journal of Community Nutrition
• /
• v.16 no.1
• /
• pp.14-22
• /
• 2011

This study was designed to compare the incidence and severity of premenstrual syndrome (PMS) between normal (N = 85) and overweight or obese (N = 28) college female students and investigated correlation between PMS, nutrient intake, hematological index and psychological index (depression, anxiety, stress). Each subject was asked a Menstrual Discomfort Questionnaire (MDQ) for PMS by 5 Likert scale. The PMS scores of women in the normal weight subjects ranked in order of severity were water retention (2.71), followed by behavioral change (2.58), negative affect (2.46), pain (2.31), autonomic reaction (2.27), decreased concentration (2.16). The symptoms of 'pain' and 'behavioral change' of overweight or obese subject were significantly higher than those of normal subject (p < 0.05). And total cholesterol concentration of overweight or obese subjects was significantly higher than in normal subject (p < 0.05). There was a significant positive correlation (p < 0.05) between the symptoms of 'negative effect' and BMI. And the triglyceride concentration was positively related with 'water retention (p < 0.01)'. The symptoms of 'decreased concentration' were negatively correlated with calcium (p < 0.01) and vitamin B6 intake (p < 0.05). The depression score were positively related with symptoms of 'behavioral change (p < 0.05)', 'negative affect' (p < 0.01), and the anxiety score was positively correlated with 'behavioral change (p < 0.05)' and 'decreased concentration (p < 0.05)'. The stress score was positively correlated with 'decreased concentration (p < 0.01)', 'behavioral change (p < 0.05)' and 'negative affect (p < 0.05)'. This suggests that PMS represents the clinical manifestation of a calcium, vitamin $B_6$ deficiency and psychological disorder. Therefore we concluded that nutrient supplementation, depression and stress management may help to relieve PMS symptoms.

• The Korean Journal of Mycology
• /
• v.34 no.2
• /
• pp.122-124
• /
• 2006

In March of 2005, gray mold disease caused by Botrytis cinereu on Stachys sieboldii Miq. was occurred in the mud cellar storage of Gyeongsangnam-do Agricultural Research and Extension Services, Korea. The symptoms started with water-soaked and rotten in the tubers. The conidia were one celled and mostly ellipsoid or ovoid in shape and light gray in color. The conidia were $5{\sim}16{\times}4{\sim}12{\mu}m$ in size and the conidiophores were $14{\sim}30{\mu}m$ in length. The pathogen formed conidia and sclerotia abundantly on PDA. The optimal temperature for mycelial growth and sclelotia formation was $20^{\circ}C$. On the basis of symptom, mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea Persoon: Fries. This is the first report on gray mold of S. sieboldii caused by B. cinerea in Korea.

• Korean Journal of Community Nutrition
• /
• v.21 no.5
• /
• pp.405-414
• /
• 2016

Objectives: The associations between the eating behavior and energy and macronutrient intake from meals and snacks consumed during different times of the day across the menstrual cycle were investigated in 74 healthy female college students. Methods: A 9-day food record was collected during the last 3 days before menstrual onset (phase 1) and the first 3 days after menstrual onset (phase 2) and from the 4th to the 6th day after menstrual onset (phase 3), respectively. Anthropometry was assessed and eating behaviors were measured using the Dutch Eating Behavior Questionnaire (DEBQ). Results: External eating was the most prevalent type of eating behavior, followed by restrained eating and emotional eating. Restrained eating was positively associated with energy, carbohydrate and lipid intake at the breakfast and midmorning snack during phase 3. However, emotional eating was also positively related to energy and macronutrient intake at the dinner and after-dinner snack during phase 1 and phase 3, with higher level detected in the phase 1. The association of emotional eating with the snack consumption was highest in phase 1. External eating was positively associated with energy and macronutrient intakes at the dinner and after-dinner snack across the three phases, the highest level being phase 1. In addition, restrained eating was positively associated with the weight, body mass index(BMI), fat mass, waist and hip girth of the subjects. Conclusions: Eating behaviors varied with regard to meals and snacks consumed during different times of the day across the three menstrual phases. Dinner and afterdinner snack consumption in premenstrual phase could be considered as a time when women are more prone to overconsumption and uncontrolled eating.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.24 no.1
• /
• pp.144-161
• /
• 2011

Objectives: Oriental Medicine has thousands years of history. But this era, every medical decision should be based on scientific evidence, that is evidence-based medicine (EBM). This study is to suggest quantitative case report form for dysmenorrhea that can be easily used in clinics. Methods: First, to search published papers in Korea and overseas, OASIS, KISS and NCBI(pubmed) database. Second, to search clinical trials in clinical trial register website, ISRCTN and clinicaltrials.gov. Result: Visual Analogue Scale(VAS) is the most commonly used scale. Conclusion: The main scales for dysmenorrhea are Visual Analogue Scale(VAS), Multidimensional verbal Rating scale(MVRS), pain-killer intake amount and restriction of daily life activities. To measure the effect of herbal medicine, more than 2 menstrual cycle is recommended.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.24 no.4
• /
• pp.50-61
• /
• 2011

Purpose: This study was performed to analyse autonomic nerve function of some examinees with dysmenorrhea by comparing the characteristics of dysmenorrhea patients' Heart Rate Variability(HRV) in examinees group with non-examinees group. Methods: We studied 110 patients visiting OO Korean hospital to take medical treatment for dysmenorrhea from September, 2006 to August, 2010. The subjects were categorized in two groups, 36 examinees and 57 non-examinees controls. We investigated the difference of HRV between two groups by student's T-test using SPSS for Windows(version 12.0). Results: 1. SDNN in examinees group is statistical significantly lower than non-examinees group. RMS-SD in examinees group is lower than non-examinees group non-statistical significantly. Mean PR in examinees group is higher than non-examinees group non-statistical significantly. 2. VLF, ln TP, ln VLF and ln HF are statistically significantly lower than non-examinees group. TP, HF and ln LF are lower than non-examinees group non-statistical significantly. LF is higher than non-examinees group non-statistical significantly. Conclusions: The results suggest that stress is related to especially examinees in dysmenorrhea patients by effecting autonomic nerve function. HRV can be useful to diagnose examinees' dysmenorrhea. The further studies would be needed to study about relation between stress, dysmenorrhea and HRV for settling this.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.24 no.1
• /
• pp.99-108
• /
• 2011

Objectives: The purpose of this study is to recognize the relation of menstrual pattern and dysmenorrhea affected by obesity of first-year female college students. Methods: From March 22th to April 2nd, 2010 we researched 251 first-year female students at OOcollege, aged 19(born after March 1991 to February 1992) by questionnaires to investigate their Body Mass Index(BMI), menstruation and dysmenorrhea. The BMI range of normal group is 18.5~25, abnormal group is over 25. Results: 1. The normal group was 227 people(90.4%) and the abnormal group(over BMI 25) was 24 people(9.6%). 2. In the mean of BMI, the normal group was $21.08{\pm}1.64$ and the abnormal group was $27.94{\pm}2.19$. 3. There was no statistical difference between the normal group and the abnormal group in menstrual cycle, menstrual amount, menstrual color and menstrual blood clot. 4. There was statistical difference between the normal group and the abnormal group in menarcheal age, menstrual duration and dysmenorrhea. 5. In the mean of VAS, the normal group was $3.68{\pm}2.45$ and the abnormal group was $3.21{\pm}2.05$, and two groups were not different statistically. Conclusion: This study showed the relation of menstrual pattern and dysmenorrhea affected by obesity. The normal group and the abnormal group were different statistically in menarcheal age, menstrual duration and dysmenorrhea. In consequence of this study, it was supposed that obesity had an effect on menstruation.