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In vitro Response of Cytochrome P450 (CYP) and EROD Activity in Bivalve (Coelomactra antiquata) by DDT, PCB-153 and TCDD (유기염소화합물인 DDT, PCB-153 및 TCDD에 in vitro 노출시킨 명주조개 미크로좀의 CYP및 EROD활성 반응)

  • 전중균;이미희;이지선;이경선;심원준;신영범;이수영
    • Korean Journal of Environmental Biology
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    • v.21 no.2
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    • pp.170-176
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    • 2003
  • The effect of xenobiotics on cytochrome P450 (CYP) and 7-ethoxyresorufin-O-deethylase (EROD) in hepatopancreatic microsome of surf clam, Coelomactra anti-quata, were investigated. The microsome isolated from the digestive gland of the surf clam, collected from the east coast of Korea, was in vitro exposed to p, p -DDT (0.1,0.4 and 1.0 mM) for 30 min and 2,3,7,8-TCDD (0.01, 0.04 and 0.1 ppb) and PCB-153 (0.01, 0.04 and 0.1 ppb) for 7 hr. In the case of DDT exposure, the CYP content and EROD activity of 1.0 mM exposure group increased up to about 117% and 120% of the DMSO solvent control group after 10 min. exposure, respectively. After 2 hr exposure of TCDD, the CYP content and EROD activity were also induced to the range of 103∼110% and 121∼139%, respectively. The PCB-153 exposure group showed 107∼115% of CYP content and 129∼140% of EROD activity after 2 hr exposure. Three test chemicals apparently induced CYP and EROD activity in the microsome of surf clam. The inducing potentials depend en the test chemicals.

Study on the Distribution of Vibrio parahaemolyticus along Cheju Coast (제주연안해역의 Vibrio parahaemolyticus 분포에 관한 연구)

  • Song, Min-Kyoung;Kim, Man-Chul;Heo, Moon-Soo
    • Korean Journal of Environmental Biology
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    • v.25 no.1
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    • pp.34-41
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    • 2007
  • A study on the distribution of V. parahaemolyticus among sea water, sea mud, and marine products in Hwabuk, Samyang, Daepo, Jungmun, Pyoson, Anduk, Aewol, and Gwakji on the coastal area of Jeju island was conducted from January to December in 2002. The 2,880 total specimens of 960 sea waters, 960 sea mud, 960 marine products were collected and studied for the rate of isolation of V. parahaemolyticus, and biochemical, serological and antibiotic sensitivity tests were performed. A total of 417 strains of V. parahaemolyticus were isolated and identified from 2,880 total specimens. In the test of biochemical properties, 100 of V. parahaemolyticus isolates in the presence of 0.85% NaCl were positive in the utilization of lysine, ornithine, indole, glucose, and mannitol, and negative in the utilization of ONPG, arginine, sodium citrate, urea, tryptophane, inositol, sorbitol, rhamnose, sucrose, and melibiose, $H_2S$ production and VP reaction, while positive or negative in gelatin liquefaction and utilization of amygdalin or arabinose. The isolation rates to the specimen were 161 strains (16.8%) from 960 of sea waters, 137 strains (14.3%) from 960 of sea mud, and 119 strains (12.4%) from 960 of marine products. The isolation rates of V. parahaemolyticus from 8 coastal areas were 14.4% (52/360) in Hwabuk area, 15.3% (55/360) in Samyang area, 13.6% (49/360) in Daepo area, 18.3% (66/360) Jungmun area, 13.1% (47/360) in Pyosun area, 16.4% (59/360) in Anduk area, 12.5% (45/360) in Aewol area and 12.2% (44/360) in Gwakji area, respectively. The distribution of 417 V. parahaemolyticus, isolates was high at Jungmun with 18.3% (66/360), and from sea water with 16.8% (161/960).

Bloom-forming dinoflagellate Akashiwo sanguinea(Dinophyceae) in Jangmok Harbour of Geoje Island, Korea: Morphology, phylogeny and effects of temperature and salinity on growth (거제도 장목항에서 적조원인생물 Akashiwo sanguinea(Dinophyceae): 형태, 분자계통학적 특성 및 온도와 염분에 따른 성장 특성)

  • Han, Kyong Ha;Li, Zhun;Youn, Joo Yeon;Kang, Byeong Jun;Kim, Hyun Jung;Seo, Min Ho;Soh, Ho Young;Shin, Hyeon Ho
    • Korean Journal of Environmental Biology
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    • v.37 no.2
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    • pp.119-128
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    • 2019
  • The morphological characteristics of the bloom-forming dinoflagellate Akashiwo sanguinea isolated from Jangmok Harbour, Geoje in Korea was examined using light and scanning electron microscope (SEM), and its large subunit (LSU) rDNA was sequenced. Additionally, investigation was done on the effects of temperature and salinity on the growth of A. sanguinea. The cells were dorso-ventrally compressed up to 54.7-70.3 ㎛ long and 31.5-48.5 ㎛ wide. The epicone was conical while the hypocone was separated into two lobes. The nucleus was positioned at the center of the cell. The yellow-brown chloroplasts radiated close to the cell center. SEM observation indicated that A. sanguinea has an e-shaped apical groove. Molecular phylogeny based on LSU rDNA gene sequences revealed that the A. sanguinea strains isolated from Jangmok Harbor were classified in the clade of ribotype A. The maximum growth rate (0.50 day-1) was observed at 20℃ and 20 psu, while the maximum cell density (1,372 cells mL-1) was observed at 25℃ and 30 psu. This indicates that the blooms of A. sanguinea ribotype A in Korean coastal area are affected by water temperature, rather than the salinity.

On the Bacterivory of Ciliates in the Estuarine System of the Mankyung and Dongjin rivers. (만경.동진강 염하수에서 섬모충류에 의한 박테리아 섭식에 관하여)

  • SHIM, JAE HYUNG;PARK, SOO YOUNG;CHO, BYUNG CHEOL;YIH, WONHO
    • 한국해양학회지
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    • v.30 no.5
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    • pp.426-435
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    • 1995
  • In order to assess the significance of ciliate grazing on bacterial population in carbon flow of the estuarine pelagic ecosystem of the Mankyung and Dongjin river (MD estuary), abundance and biomass of ciliates and grazing rate on bacteria of small (<40 um) ciliates were measured. Saplings were carried out four times from October 1993 to March 1995 in the estuarine system. Ciliates smaller than 40 um occupied more than 49.5% of total ciliates abundance. Clearance rate of small ciliates ranged from 18.0 to 16.3 nl cell/SUP -1/ h/SUP -1/. As bacterial abundance increased, ciliate's clearance rate decreased, suggesting that bacterial abundance effects on ciliate's grazing rate. Ciliate grazing rate was equal to 0.1 to 12.2% of bacterial productivity, and the ratio of ciliate ingestion over bacterial production increased exponentially with the increase of bacterial abundance (r$^2$=0.62, p$\leq$0.001). It seems that the effect of ciliate grazing on bacteria would be small in coastal waters and large in more eutrophic waters of high bacterial abundance. Carbon supplied by ciliate grazing on bacteria was avg. 3.1% of carbon amount required for the ciliate maximum productivity. Thus, the ecological role of ciliate in microbial loop was probably more important as a final consumer than as a direct consumer of bacteria.

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Characteristics of Heavy Metal Distribution in Surface Sediments from the south Sea of Korea (남해 대륙붕 표층퇴적물 중 중금속 원소의 분포 특성)

  • 조영길;이창복
    • 한국해양학회지
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    • v.29 no.4
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    • pp.338-356
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    • 1994
  • Sediment samples from the continental shelf of the South Sea of Korea are analysed to determine the concentration of Al, Fe, Mn, cu, Ni, Zn, Co, Cr and Pb. among these samples, fine-grained sediments were also analysed by a sequential extraction technique to know geochemical forms of the metals in this environments. The total concentration of Al, Fe, Cr, Ni, Cu and Zn in bulk sediments decreased gradually with the increase of distance from the coastal zone. This distribution patterns are well coincide with grain size distribution. However, the patterns of Mn, co and Pb do not follow such and overall distribution. The concentration of Pb, particularly, did not show any features in areal distribution, which may be result from different pathways to the sediments, compared to the other metals. the speculation data show that a considerable amount of Cd, Mn and Co are bounded in the carbonate fraction, accounting for 42.8%, 40.3% and 30.6%, respectively. Cu, Zn, Mn and Fe are largely associated to oxide fraction with proportions of 34.4%, 23.1%, 15.5% and 13.7%, respectively. However, the metals in residual fraction account for more than 50% of the total metal concentration, except for Mn. These observations emphasize that residual fraction in the dominant component controlling the elemental concentration.quartz and glauconite grains.Accordingly,these sediments are interpreted as an extension part of transgressive sand deposit that are widely distributed on the continental shalf floor of southern Yellow Sea.

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Physical Structure of Eddies in the Southwestern East Sea (동해남서해역 와류의 물리적구조)

  • 이흥재;변상경
    • 한국해양학회지
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    • v.30 no.3
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    • pp.170-183
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    • 1995
  • Eddies and surface current field in the southwestern part of the East Sea were investigated using satellite-tracked drifters, CTD, and ADCP from November 1992 to September 1993. Trajectories of surface drifters provided information for the first time on the meandering motion of the East Korean Warm Current in the Ullung Basin (referred as UB) and clearly indicated the existence of cyclonic and anticyclonic eddies of various scales. Anticyclonic eddies persisting for a relatively long period were observed in UB and the southwestern corner of the Northern (Japan) Basin (SNB), while a cyclonic eddy was found in the coastal area between Sokcho and Donghae during the summer. Analysis shows that the eddy in UB behaved as a stationary eddy at least during the observation period and the cyclonic eddy was closely related to the existence of a cold water mass. The anticyclonic eddy in SNB was larger than that in UB, but much elongated in shape. The eddy in UB is characteristic of major and minor axes of about 120 and 70 km, revolution period of 13.6 days, mean swirl velocity of about 24 cm/s, and mean eddy kinetic energy of 392 cm$\^$2//s$\^$2/. The eddy in SNB is described as follows; major and minor axes of 168 and 86 km, period of 14.9 days, mean swirl velocity of 29 cm/s and mean eddy kinetic energy of 629 cm$\^$2//s$\^$2/. The mean translational speed is about 3 cm/s for both eddies. The agreement of the surface current pattern in UB observed by ADCP with the geostrophic flow pattern may suggest that the eddy in UB was nearly in geostrophic balance. The eddy was found to be strongly bottom-controlled.

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The Ecological Study of Phytoplankton in Kyeonggi Bay, Yellow Sea. III. Phytoplankton Composition, Standing Crops, Tychopelagic Plankton. (西海 京畿 植物 플랑크톤에 대한 생態學的 硏究 III. 植物플랑크톤 種조성, 現存量, 일시浮流플랑크톤)

  • 최중기;심재형
    • 한국해양학회지
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    • v.21 no.3
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    • pp.156-170
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    • 1986
  • The phytoplankton ecology of estuarine waters was investigated in the Kyeonggi Bay from May 1981 to September 1982 on monthly basis. In this study area, a total of 228 phytoplankton species was identified. Among these taxa, the most dominant species are diatoms in this area. Tychopelagic plankton occupies 40.4% of total species. The percentage of tychopelagic plankton density ranged from 10.2% in September to 92.7% in March of monthly standing crops. From late autumn to early spring, the percentage values are more than 72%. They play an important role from late autumn to early spring in this estuarine plandton community. These tychopelagic planktons are induced from benthic diatoms. Because the bottom shear stresses generated by the tides and winds are stronger than the adhesive and tractive force of benthic diatoms, most of benthic diatoms must be resuspended into tychopelagic suspensions during autumn and winter. Paralia sulcata is the most important tychopelagic plankton as an indicator species of water mixing in the eastern coastal area of Yellow Sea. This species seems to have even broader tolerance to the environmental stress than Skeletonema costalum, and tends to fill the gaps in winter, when the phytoplandton is relatively unsuccessful. Skeletonema costatum and Chaetoceros debilis are dominant in other seasons. Typical blooms of phytoplankton occur in spring and early autumn, The first bloom is started by Skeletonema costatum in early May, second peak is formed by various diatom population in September.

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On the Abnormal Low Temperature Phenomenon of the Yellow Sea Bottom Cold Water in Summer, 1981 (1981年 夏季 黃海底層冷水의 理想底水溫現象)

  • Yang, Sung-Ki;Cho, Kyu-Dae;Hong, Chol-Hoon
    • 한국해양학회지
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    • v.19 no.2
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    • pp.125-132
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    • 1984
  • The abnormal low water temperature phenomenon of the Yellow Sea Bottom Cold Water in summer, 1981 is studied on the basis of the oceanographical data collected by the National Fisheries University of Pusan in July, 1981 and Fisheries Research and Development Agency of Korea from 1960 to 1981 and meteorological data within the same 22 years. In winter, 1980 the northwesterly monsoon was vary predominant and the air temperature was lower than that of mean year by 1∼8$^{\circ}C$ and also the surface temperature was lower 1∼3$^{\circ}C$. And then the temperature of the Yellow Sea Bottom Cold Water in summer, 1981 became lower 2∼3$^{\circ}C$ than that of mean year and the influence of this cold water was extended to about 50 miles off the coast of Cheju Island. Comparing with mean year, the water temperature at 30m depth in February, 1981 was lower by 1∼2$^{\circ}C$ in entire regions except near sea of Sohuksando and at 50m depth in August, 1981, it was lower by about 3.5$^{\circ}C$. Particularly, the offshore of Hongdo shower value of 5$^{\circ}C$ than that of mean year. It was found that the abnormal low water temperature phenomenon of Yellow Sea Bottom Cold Water in summer, 1981 resulted from the sea surface cooling by the predominant northwestly monsoon and abnormally low air temperature in winter, 1980.

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Variations in Plankton Assemblage in a Semi-Closed Chunsu Bay, Korea (반폐쇄적인 천수만 해역의 플랑크톤 군집 변화)

  • Lee, Jae-Kwang;Park, Chul;Lee, Doo-Byoul;Lee, Sang-Woo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.17 no.2
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    • pp.95-111
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    • 2012
  • Relationship between plankton assemblage and environmental factors in a semi-closed Chunsu Bay was examined. Temporal changes in phytoplankton assemblage was rather drastic than those found in most Korean coastal area in the Yellow Sea primarily due to the seawater temperature (T) and nutrient input from the dikes nearby. Freshwater discharge seemed to cause winter time increase of Diatoms (February) and summer time increase of Dinoflagellates at surface (July to August). Structural change in cell size with time was also found in Diatom. Zooplankton community structure was also changed with season probably due to the food concentration, seawater temperature and salinity (S). From principal component analysis (PCA) of zooplankton distribution, it was postulated that seasonal environmental changes such as T and S could explain about 32% of variability in zooplankton distribution along with phytoplankton cell numbers, while freshwater discharge could explain about 17%. Comparing with past data of 1985-1986, 1991-1992, the distributional patterns and percent composition of major species, Acartia hongi, Paracalanus parvus sensu lato and Centropages abdominalis, were similar. However, the abundances have been increased more than three times. The composition of other taxa than copepods showed significant changes.

Upwelling-Response of the Cold Water off Haeundae in Summer (여름철 해운대 냉수대의 용승반응)

  • Lee, J.C.
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.16 no.4
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    • pp.206-211
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    • 2011
  • Low water temperature during the summer associated with the occurrence of cold water zone off Haeundae was studied using the data from CTD observations and a monitoring buoy deployed in Suyeong Bay. Shortterm variability of current was dominant and was not related to the wind. The NE-SW components of wind parallel to the coast contained more than 96% of total variance and could account for major fluctuations of water temperature. Upwelling-response of water temperature was very sensitive so that the temperature began to decrease immediately after the onset of the southwesterly wind. In particular, there were three cases in which SW winds for only two days caused considerable temperature drops. In 2009, four upwelling events shorter than 5 days took place while seven events with periods of 2~18 days were recorded in 2010. During a very intense upwelling for seven days in mid-August 2010, temperature decreased by more than $10^{\circ}C$ in spite of the variable winds. Temperature variability at Gampo, Ulsan, Gijang and Haeundae had similar patterns. CTD observation and satellite imagery showed that the upwelling zone could be extended to the Haeundae-Busan area. According to the wavelet analysis, coherent periods were 2~8 days during the frequent upwelling/downwelling events.