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The relation between angiotensin converting enzyme (ACE) gene polymorphism and neonatal hyperbilirubinemia in Korea (한국인 신생아 황달과 안지오텐신 전환효소 유전자의 다형성)

  • Kim, Mi Yeoun;Lee, Jae Myoung;Kim, Ji Sook;Kim, Eun Ryoung;Lee, Hee Jae;Yoon, Seo Hyun;Chung, Joo Ho
    • Clinical and Experimental Pediatrics
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    • v.50 no.1
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    • pp.28-32
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    • 2007
  • Purpose : Human angiotensin converting enzyme (ACE) gene shows an insertion/deletion polymorphism in 16 intron, and three genotypes are determined by whether a 287 bp fragment of the DNA is present or not; II, ID and DD genotype. DD genotype has been suggested as a risk factor of chronic nephrotic disease such as IgA nephropathy and diabetic nephropathy, various cardiovascular diseases and several other diseases. ACE activity increases in acute hepatitis, chronic persistent hepatitis, chronic active hepatitis and cirrhosis. On the other hand, patients with fatty livers have normal ACE activity. This study was designed to find out the relation between polymorphsims of the ACE genes and neonatal hyperbilirubinemia in Koreans. Methods : The genomic DNA was isolated from 110 full-term Korean neonates who had hyperbilirubinemia with no obvious causes (serum bilirubin$${\geq_-}12mg/dL$$) and 164 neonates of a control population (serum bilirubin <12 mg/dL). We performed polymerase chain reaction (PCR) to see the allele of the ACE gene. Electrophoresis was done in the PCR products in 1.5 percent agarose gel, and then DNA patterns were directly visualized under ethidium bromide staining. Results : ACE genotypes in the hyperbilirubinemia group are as follows; 26.36 percent for II, 53.64 percent for ID, 20.00 percent for DD, 0.532 for I allele and 0.468 for D allele. These distributions were not significantly different from those in the control group; 24.39 percent for II, 51.83 percent for DI, 23.78 percent for DD, 0.503 for I allele and 0.497 for D allele. Conclusion : In this study, ACE gene polymorphism was detected in the neonatal hyperbilirubinemia and control group. The most frequent genotype was ID. Our results indicate that the ACE gene polymorphism is not associated with the prevalence of neonatal hyperbilirubinemia in Koreans.

Results of radiofrequency catheter ablation in children and adolescent with tachyarrhythmia (소아 청소년 부정맥 환자에서 고주파 전극도자 절제술의 이용 성적)

  • Chang, Young Beom;Lee, Seung Hyun;Kang, Eun Young;Rhee, Kyoung-Suk;Joo, Chan Uhng
    • Clinical and Experimental Pediatrics
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    • v.50 no.11
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    • pp.1085-1090
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    • 2007
  • Purpose : Radiofrequency catheter ablation (RFCA) has become an effective therapeutic modality for treating pediatric tachyarrhythmias. Using conventional RFCA catheters, ablation of parahisian accessory pathways may be difficult and have high risk for heart block. We reviewed the efficacy and complications of the RFCA in children and adolescent with arrhythmias including parahisian accessory pathways. Methods : We studied 48 patients (aged 2 years to 20 years) who had undergone RFCA from August 2003 to March 2007. We reviewed clinical findings, electrophysiologic studies, RFCA data, complications, and follow-up results of the patients. Results : Mean age of the patients was 13.1 years. Numbers and types of arrhythmias (age, acute success rate) were as follows: 19 WPW syndrome including 5 parahisian accessory pathways ($13.7{\pm}4.6yr$, 18/19), 11 atrioventricular reentrant tachycardia with concealed bypass tract ($12.3{\pm}5.0yr$, 10/11), 13 atrioventricular nodal reentrant tachycardia ($12.6{\pm}4.4yr$, 13/13), 4 atrial flutter ($13.0{\pm}7.4yr$, 3/4), and 1 ventricular tachycardia (20 yr, 1/1). Associated cardiac structural lesion was not detected in 48 patients. The recurrence rate was 6.5%, and the final success rate was 93.8%. Conclusion : These results suggest that RFCA is a highly effective treatment method in children and adolescent with tachyarrhythmia.

Studies on the Development of Food Resources from Waste Seeds -II. Chemical Composition of Apple Seed- (폐기종실(廢棄種實)의 식량자원화(食糧資源化)에 관(關)하여 -제(第) 2 보(報) : 사과씨의 화학적(化學的) 조성(組成)-)

  • Yoon, Hyung-Sik;Choi, Cheong;Oh, Man-Jin
    • Korean Journal of Food Science and Technology
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    • v.15 no.2
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    • pp.128-132
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    • 1983
  • The apple seed contained 25.96% of crude fat and 37.62% of crude protein. The lipid fractions obtained by cilicic column chromatography were mainly composed of about 93.52% neutral lipid, whereas compound lipid was only 6.48% level. Among the neutral lipid separated by thin layer chromatography, triglyceride was 92.17%, sterol ester, sterol, diglyceride and free fatty acid were 3.53, 2.25, 1.44 and 0.56, respectively. The predominent fatty acids of total and neutral lipids were linoleic acid (59.79-69.37%) and oleic acid (20.04-29.82%), but those of glycolipid and phojspholipid were linoleic acid (29.20-36.04%). The major fatty acids of triglyceride separated from neutral lipid were oleic acid (44.31%), linoleic acid (36.66%) and palmitic acid (12.48%). The salt soluble protein of apple seed was highly dispersible in 0.02M sodium phosphate buffer containing about 1.0M $MgSO_4$, and the extractability of seed protein was 37%, Glutamic acid was the major amino acid in salt soluble protein, followed by arginine and aspartic acid. The eletrophoretic analysis showed three bands in apple seed protein, and the collection rate of the main protein fraction purified by Sephadex G-100 and G-200 was 76.6%. Glutamic acid, aspartic acid and arginine were the major amino acids of the main apple seed protein. The molecular weight for the main protein of the apple seed was estimated to be 45,000.

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TIC SYMPTOM EXACERBATION ASSOCIATED WITH STREPTOCOCCAL INFECTION IN TOURETTE'S DISORDER (A군 연구균 감염이후 Antistreptolysin-O 혈중 농도 증가가 뚜렛씨 장애의 증상악화에 미치는 영향에 대한 임상 연구)

  • Joung, Yoo-Sook
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.9 no.2
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    • pp.209-217
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    • 1998
  • Object:The purpose of this study was to investigate that infection with group $A[{\beta}]$ hemolytic streptococcus may associate the mechanisms that cause or exacerbate the tic symptoms in some cases of Tourette's disorder Method:Fourteen cases with abrupt worsening of tics participated in this study:10 males,4 females. The subjects were divided into two groups composing of the group with increasing level of ASO titer and the group with normal level of ASO titer. The subjects were administered Yale Global Tic Severity Scale(YGTSS). Result:The global severity scores and overall TS impairment rating scores of YGTSS in the group with increasing level of ASO titer were more higher than in the group with normal level of ASO titer Conclusion:These results suggest that increasing level of ASO titer, resulting from group $A[{\beta}]$ hemolytic streptococcal infection has affected worsening the tic symptoms in Touette's disorder.

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Effect of Different Stunning Time on Meat Quality of Broiler (육계 도축 시 전기 실신 시간이 육질에 미치는 영향)

  • Chae, H.S.;Ahn, C.N.;Yoo, Y.M.;Ham, J.S.;Jeong, S.G.;Lee, J.M.;Choi, Y.I.
    • Journal of Animal Science and Technology
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    • v.47 no.6
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    • pp.1017-1024
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    • 2005
  • This study was investigated the effect of the length of stunning time on pH, water holding capacity(WHC), cooking loss(CL), meat color and incidence rate of blood spot in broiler carcass. One-hundred and forty broiler chickens were slaughtered by three different length of stunning times(5 sec., 8 sec., 11 sec.) with the same electrical frequency(255Hz) and 50 voltage in a commercial abattoir. The WBS values and cooking loss of breast muscle were increased with increasing the stunning time, while WHC of breast muscle were decreased. Lightness(L* value) and yellowness(b* value) scores of leg muscle and skin stunned with 50V, 255Hz, 8 sec, 11 sec. were higher than those of broilers stunned with 50V, 255Hz, 5sec(P<0.05). But, there was no significant difference in color on breast and wing muscle. In subjective evaluation, frequence of the first grade had a trend of being increased by extending the stunning time. There was only a few cases of PSE chicken with 0.02%, while blood spot was observed at the highest rate for the 5 sec. treatment. TBARS indicated that a longer length of stunning resulted in a higher rate of fat oxidation. This experiment demonstrated that the length of stunning time has a significant effect on meat quality and its stability during chiller storage.

Hydrochemistry and noble gas origin of hot spring waters of Icheon and Pocheon area in Korea (이천 및 포천지역 온천수의 수리화학적 특성 및 영족기체 기원)

  • Jeong, Chan-Ho;Koh, Yung-Kwon;Shin, Seon-Ho;Nagao, Keisuke;Kim, Kyu-Han;Kim, Gun-Young
    • The Journal of Engineering Geology
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    • v.19 no.4
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    • pp.529-541
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    • 2009
  • Hydrochemical, stable isotopic ($\delta^{18}O$ and dD) and noble gas isotopic analyses of seven hot spring water samples, eleven groundwater samples and six surface water samples collected from the Icheon and Pocheon area were carried out to find out hydrochemical characteristics, and to interpret the source of noble gases and the geochemical evolution of the hot spring waters. The hot spring waters show low temperature type ranging from 21.5 to $31.4^{\circ}C$ and the pH value between 6.69 and 9.21. Electrical conductivity of hot spring waters has the range from 310 to $735\;{\mu}S/cm$. Whereas the hot spring water in the Icheon area shows the geochemical characteristics of neutral pH, the $Ca-HCO_3$(or $Ca(Na)-HCO_3$) chemical type and a high uranium content, the hot spring water in the Pocheon area shows the characteristics of alkaline pH, the $Na-HCO_3$ chemical type and a high fluorine content. These characteristics indicate that the hot spring water in the Icheon area is under the early stage in the geochemical evolution, and that the hot spring water in the Pocheon area has been geochemically evolved. The $\delta^{18}O$ and ${\delta}D$ values of hot spring waters show the range of $-10.1{\sim}-8.69%o$ and from $-72.2{\sim}-60.8%o$, respectively, and these values supply the information of the recharge area of hot spring waters. The $^3He/^4He$ ratios of the hot spring waters range from $0.09\;{\times}\;10^{-6}$ to $0.65\;{\times}\;10^{-6}$ which are plotted above the mixing line between air and crustal components. Whereas the helium gas in the Icheon hot spring water was mainly provided from the atmospheric source mixing with the mantle(or magma) origin, the origin of helium gas in the Pocheon hot spring water shows a dominant crustal source. $^{40}Ar/^{36}Ar$ ratios of hot spring water are in the range of an atmosphere source.

Detection of Copy Number Variation of the KIT Gene in the Landrace Breed using an Quantitative Oligonucleotide Ligation Assay(qOLA) (Quantitative Oligonucleotide Ligation Assay(qOLA)를 이용한 Landrace 품종의 KIT 유전자 반복수 변이 탐지)

  • Seo, B.Y.;Kim, J.H.;Nahm, D.W.;Yoo, C.K.;Lee, S.H.;Lee, J.B.;Lim, H.T.;Jung, E.J.;Cho, I.C.;Heo, K.N.;Jeon, J.T.
    • Journal of Animal Science and Technology
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    • v.49 no.5
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    • pp.559-568
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    • 2007
  • Recently, copy number variations (CNV) of genes or genomic segments have been intensively studied and various analysis methods have been developed. In this study, quantitative oligonucleotide ligation assay (qOLA) was applied to investigate CNV of KIT gene in the Landrace breed. A combined assay using qOLA and pyrosequencing, 6 genotype classes, I1/I1 or I3/i (IBe), I1/I2 or I3/IP, I1/I3, I1/IP or I2/i (IBe), I2/I2and I2/IP, were identified from 44 Landrace pigs. Genotype assignment using grouping features of measurements on a scatter plot showed 100% agreement with those using a statistical assignment by PROC FASTCLUS procedure implemented in the SAS package. Two versions (3100 and 3130) of ABI sequencers gave the same genotyping results, indicating there was no influence on qOLA by different versions of instrument, however, the means of standard deviation and coefficient of variation from the qOLA on a ABI 3130 (2.33 and 4.10) was lower than those from the qOLA on a ABI 3100 (2.67 and 4.81). Effect of proteinase K treatment on the PCR product followed by qOLA was very clear because noise peaks were disappeared and the observed ration fit better to the reference ratio corresponding to each genotype.

Environmental Studies in the Lower Part of the Han River Vl. The Statistical Analysis of Eutrophication Factors (한강 하류의 환경학적 연구 Vl. 부영양 요인의 통계적 해석)

  • Jung, Seung-Won;Hue, Hoi-Kwon;Lee, Jin-Hwan
    • Korean Journal of Ecology and Environment
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    • v.37 no.1 s.106
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    • pp.78-86
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    • 2004
  • In order to reveal the relationship between the concentration of chlorophyll- a and the environmental factors affecting eutrophication, the present study was biweekly conducted at G stations in the lower part of the Han river during the period from Feb. 24,2001 to Feb. 9,2002. Water temperature was changed from $0.5^{\circ}C$ to $26.4^{\circ}C$, pH was 5.77${\sim}$8.99, DO 3.15${\sim}$14,36 mg $L^{-1}$, BOD 0.90${\sim}$7.45 mg $L^{-1}$, and COD 1.16${\sim}$9.13 mg $L^{-1}$. TN and TP were ranged from 1.68${\sim}$20.96 mg $L^{-1}$, and 0.02 ${\sim}$ 1.17 mg $L^{-1}$, respectively. $NH_4\;^+$-N, $NO_3\;^-$-N, and $PO_4\;^{3-}$-P were ranged from 0.56${\sim}$3.60 mg $L^{-1}$, 0.03${\sim}$7.29 mg $L^{-1}$, and 0.002${\sim}$0.754 mg $L^{-1}$. Chlorophyll- a was extensively changed from 2.29 ${\mu}g\;L^{-1}$ to 136.28 ${\mu}g\;L^{-1}$ by month and stations. Results of nutrients indicated the eutrophic level in this area and water quality was the gradual worsening in the lower stations than those of upper stations during the period studied. The Pearson correlation analysis between the concentration of chlorophyll- a and the environmental factors indicated that BOD, COD, pH, $NH_4\;^+$-N, TP, TN, conductivity and $PO_4\;^{3-}$-P were positive correlation, but $NO_3\;^-$-N was negative. The environmental factors investigated using the principal component method could be triparted. The first factor group included conductivity, BOD, COD, TN, TP, $NH_4\;^+$-N, $PO_4\;^{3-}$-P and SS, the second WT and DO, and the third pH and $NO_3\;^-$-N. Using the stepwise regression analysis, chlorophyll- a was under the influence of conductivity, $PO_4\;^{3-}$-P, $>NO_3\;^-$-N and $NH_4\;^+$-N Chlorophyll-a = 0.3661 ${\times}$ (Conductivity) - 0.3592 ${\times}$ ($PO_4\;^{3-}$-P) - 0.3449 ${\times}$ ($NO_3\;^-$-N)+0.4362 ${\times}$ ($NH_4\;^+$-N.

Isoflavone Contents in Some Varieties of Soybean and on Processing Conditions (콩의 종류와 가공 조건에 따른 isoflavone의 함량 변화)

  • 문보경;전기숙;황인경
    • Korean journal of food and cookery science
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    • v.12 no.4
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    • pp.527-534
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    • 1996
  • enistein (G), and daidzein (D), the major isoflavones, were analyzed in 14 varieties of Korean soybean and various processed soybean products by using high performance liquid chromatography. Isoflavone contents (G+D) were greatly variable among varieties ranged from 308.2 $\mu\textrm{g}$/g to 1,134.2 $\mu\textrm{g}$/g and highest in Danyopkong and Jinpumkong. Among hypocotyl, cotyledon and hull of soybean the concentration of the isoflavone (G+D) in the hypocotyl was highest ranged from 2,971.7 $\mu\textrm{g}$/g to 5,704.9 $\mu\textrm{g}$/g. The distributions of genistein and daidzein were also different in hypocotyl, cotyledon and hull. Higher ratio of daidzein to genistein (D/G) was found in the hypocotyl (4-12) compared to cotyledon and hull (0.1-4). Isoflavone (G+D) contents of soymilks (Sinpaldal#2, Eunhakong) prepared at 16 hour hydration were decreased to 1.1-1.2 times compared with that at 8 hour hydration. Commercial soymilks contained much lower isoflavone (G+D) than laboratory soymilks. Soybean curd (Eunhakong) prepared with MgCl$_2$ showed higher isoflavone (G+D) contents than that with CaSO$_4$. But these values of two different soybean curds made at laboratory were similar to those of 3 commercial curds. The concentration of the isoflavones in soybean sprout separated with 3 parts revealed highest in the head and lowest in the stem. Compared with non-fermented soybean foods the fermented soybean produfts, Kochujang and soybean paste, Duen Jang, showed very low contents of isoflavone (G+D),2.8-3.0 $\mu\textrm{g}$/g, 35.9-63.6 $\mu\textrm{g}$/grespectively.

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Characterization of the DGAT1 Gene in the Korean Holstein Dairy Cattle Population (한국 Holstein종 유우집단의 DGAT1 유전자의 특성분석)

  • Son, Ji-Young;Jeong, Hang-Jin;Yu, Seong-Lan;Lee, Jun-Heon;Do, Chang-Hee;Ryoo, Seung-Heui;Sang, Byung-Chan
    • Korean Journal of Agricultural Science
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    • v.36 no.2
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    • pp.167-177
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    • 2009
  • This study was conducted to characterize the DGAT1 gene in Korean Holstein dairy cattle population and examine the relationship of DGAT1 polymorphisms with milk yield and milk fat yield for the genetic improvement of Korean Holstein dairy cattle. Results indicated that the 411 bp PCR products were successfully amplified by DGAT1 specific primers. Sequence analysis indicated that the DGTA1 Q allele had AUG (Lysine, K) nucleotide sequences in 216-218 bp and q allele had GCG (Alanine, A) sequences in the same position. Nucleotide sequence homology between the DGAT1 sequences generated in this study showed 100% homology with bovine DGAT1 sequences in the NCBI database. The genotype frequencies of DGAT1 QQ, Qq, and qq were 16.43, 36.43, and 47.14%, respectively, in Korean Holstein dairy cattle population. The observed Q and q allele frequencies were 0.35 and 0.65, respectively. Statistically significant (P<0.05) results were identified for milk yield and milk fat yield for the DGAT1 genotypes. The Qq genotype Holsteins have significantly higher milk yield and milk fat yield than those of the QQ and qq genotype Holsteins(P<0.05).

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