• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.4
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• pp.293-299
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• 1987

At various times and places all over the world men have become ill and some have died after eating shellfish that were intoxicated with paralytic shellfish poison(PSP) caused by Protogonyaulax spp. In late March, 1986, two persons were dead by ingesting wild sea mussels, Mytilur edulis, grown at bottom of an anchored waste ship to be dismantled at Gamchun Bay, Pusan, Korea. The samples were collected from the bottom of the ship during April $1\~April$ 8 of the year to find the cause of the food poisoning accident. The toxicity was estimated by bioassay with ICR male mouse, while the toxins were extracted and characterized. The toxins were extracted with acidified $80\%$ ethanol. The extract was defatted three times with dichloromethane, treated with activated charcoal, and then purified by chromatography on Bio-Gel P-2 and Bio-Rex 70. The toxic fractions obtained were analysed by cellulose acetate membrane electrophoresis, thin layer chromatography and high performance liquid chromatogaphy. The range and the average of PSP-toxicity of the samples were $132\~295\;MU/g$, 203 MU/g respectively. The amount of PSP was $26.4\~58.9{\mu}g/g$ of whole meat in range and $40.6{\mu}g/g$ in average. The toxicity of the digestive gland of the samples was 9 times higher than that of edible meat (except digestive gland) as $439\~979MU/g$, and it was about $70\%$ in total toxin. The compositional analytical results of the paralytic shellfish toxin, Gonyautoxin $1\~4$ were the major part of the PSP and Saxitoxin and neosaxitoxin were detected as the minor component. It was concluded that the food poisoning accident was caused not by Saxitoxins but by Gonyautoxins.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.4
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• pp.300-307
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• 1987

The present study was investigated on the DNA damage by the peroxidation of polar and non-polar lipid fractionated from mackerel lipid to elucidate the DNA damage mechanism by fish oil peroxidation. The degree of DNA damage by polar lipid peroxidation became greater with the increase of its concentration, and such DNA damage was induced below 100 millieq./kg in POV for 4 days incubation. Among the polar lipid peroxidation products, singlet oxygen $^1O_2$ and superoxide anion ${\cdot}O_2^-$ greatly affected to the DNA damage than hydrogen peroxide $H_2O_2$ and hydroxyl radical ${\cdot}OH$. Non-polar lipid peroxidation also induced the DNA damage with the increase of its concentration, but such effect was lower than the case of total lipid and polar lipid. And, the effects of active crygens on the DNA damage by non-polar lipid peroxidation was the same as in the case of total and polar lipid peroxidation.

• Journal of the Korea Convergence Society
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• v.2 no.2
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• pp.47-56
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• 2011

Korea Ministry of Knowledge Economy has estimated that wind power (WP) will be occupied 37% in 2020 and 42% in 2030 of the new energy sources, and also green energies such as photovoltaic (PV) and WP are expected to be interconnected with the distribution system because of Renewable Portfolio Standard (RPS) starting from 2012. However, when a large scale wind power plant (over 3[MW]) is connected to the traditional distribution system, protective devices (mainly OCR and OCGR of re-closer) will be occurred mal-function problems due to changed fault currents it be caused by Wye-grounded/Delta winding of interconnection transformer and %impedance of WP's turbine. Therefore, when Double-Fed Induction Generator (DFIG) of typical WP's Generator is connected into distribution system, this paper deals with analysis three-phase short, line to line short and a single line ground faults current by using the symmetrical components of fault analysis and PSCAD/EMTDC modeling.

• Journal of the Korean Geosynthetics Society
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• v.13 no.3
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• pp.1-10
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• 2014

Several types of methods such as resistivity survey, ground penetration radar, etc are used for detection of levee leakage and according to the river design guidelines detection of levee leakage is performed by measuring the hydraulic conductivity of levee soil. But, the former can not verify the leakage point and degree of saturation, the latter is an after treatment method. Movable sensor, which is a high-tech TDR system developed since 2000, can obtain directly the dielectric constant profile covering the whole depth of levee. In this study, laboratory and field model experiments were carried out using movable TDR sensor in order to evaluate the applicability as detection system of levee leakage, As the result, movable TDR system has proven to be 3 times more sensitive to water contents than dry unit weight, and the results conclude that the dielectric constant, water contents and density of the ground proved to have a correlation among them, and the dielectric constant is expected to be a basic data on detection of levee leakage.

• Journal of Periodontal and Implant Science
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• v.29 no.4
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• pp.821-838
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• 1999

On the basis of the evidences that electrical stimulation could enhance proliferation and differentiation of bone cells and promote healing and regeneration of bone, this study was performed to investigate the effects of electrical stimulation on human periodontal ligament cells and gingival fibroblasts in vitro, which also have important roles in regeneration of periodontium, and to evaluate the potential of clinical application of electrical stimulation. Human periodontal ligament cells and gingival fibroblasts were primarily cultured from the root surface of extracted premolar and the adjacent gingiva without periodontal diseases. In control group, the cells ($5{\times}10^4$ cells/ml)were incubated only in Dulbecco's Modified Eagle's Medium contained with 10% fetal bovine serum. In test groups, electrical stimulation was given at the current intensity of $0.25{\mu]A$(test group 1), $1.0{\mu}A$(test group 2), and $2.5{\mu}A$(test group 3) for 12 hours to the same culture media with the control group. After 12 hour exposure of electrical stimulation, the cells were incubated for 2 and a half days(60 hours), and then each group of cells was analyzed for cell proliferation, protein level, and activity of alkaline phosphatase. The results were as follows ; 1. The Rate of cell proliferation of every test group increased significantly in both periodontal ligament cells and gingival fibroblasts, and in periodontal ligament cells, test group 3 showed significantly increased proliferation compared to the other test groups(p<0.05). 2. In the protein levels, neither periodontal ligament cell nor gingival fibroblast showed statistically significant differences between control and test groups. 3. The activity of alkaline phosphatase in periodontal ligament cells increased significantly in all test groups(p<0.05), but there were no significant differences between 3 test groups. In gingival fibroblasts, the activity of alkaline phosphatase increased significantly only in test group 3(p<0.05). From the above results, it is concluded that electrical stimulation may have beneficial effects on the regeneration of destructed periodontal tissue in regard of the stimulation of periodontal ligament cells and gingival fibroblasts as well as electrically stimulated bone formation that has been known, and that electrical stimulation may have the potential of clinical application.

• Journal of the Korean Society of Combustion
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• v.21 no.3
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• pp.1-6
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• 2016

The effect of applied electric fields on jet flow instability was investigated experimentally by varying the direct current (DC) voltage and the alternating current (AC) frequency and voltage applied to a jet nozzle. We aimed to elucidate the origin of the occurrence of twin-lifted jet flames in laminar jet flow configuration, which occur when AC electric fields are applied. The results indicate that a twin-lifted jet flames originates from cold jet instability, caused by interactions between negative ions in the jet flow via electron attachment as $O_2+e{\rightarrow}O_2{^-}$ when AC electric fields are applied. This was confirmed by experiments in which a variety of gaseous jets were ejected from a nozzle to which DC voltages and AC frequencies and voltages were applied, with ambient air between two deflection plates connected to a DC power source. Experiments in which jet flows of several gases were ejected from a nozzle and AC electric fields were applied in coflow-nitrogen provided further evidence. The flow instability occurred only for oxygen and air jets. Additionally, jet instability occurred when the applied frequency was less than 80 Hz, corresponding to the characteristic collision response time. The effect of AC electric fields on the overall structure of the jet flows is also reported. Based on these results, we propose a mechanism to reduce jet flow instability when AC electric fields are applied to the nozzle.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.11
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• pp.753-757
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• 2014

Anode electrode in a microbial fuel cell (MFC) should transfer the receiving electron as well as provide large surface area that can be immobilized microorganisms. Microorganisms' population is one of important factors to improve the current generation and to treat the livestock wastewater by biological treatment. These studies were attempted to investigate if stainless-steel wire skein (SSWS) could be used as anode electrode replacement a graphite felt electrode in microbial fuel cell. For these studies, pretreated livestock wastewater was used diluted to 500 mg/L as COD before use. At this time, the current showed a little difference of about 5% when using each of a SSW and graphite felt (control). There was no significant difference in the current value. The organic removal rate in the microbial fuel cells used graphite felt and SSWS was 82.4% and 88.3%, respectively. The COD removal in the MFC used the SSWS was higher than that of graphite felt. Ammonium nitrogen was showed similar trend in two case all. These results about current generation and organic matter reduction seem possible that SSWS was used to anode electrode. When SSWS is used, the initial investment for system construction is expected to be able to reduce by approximately 1/50.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.6
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• pp.311-317
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• 2017

The aim of this study is to investigate the environmental properties of paddy soils depending on farming methods and seasonal variation. The paddy soils in 11 plots of conventional paddy and 24 plots of organic paddy were sampled and analyzed in four season of March, May, August, and October. The obtained data of soil properties were used for statistical analysis. Analysis of variance showed that only $NH_4-N$ and $P_2O_5$ were significantly different depending on farming methods. However, the differences of all soil properties depending on seasonal variation were strongly significant. Principal component analysis also presented that nitrogen and phosphorus concentration in soils were more significantly influenced by seasonal variation than farming method. Electric conductivity in soil was decreased from March to October. Amounts of soil organic matter in August and October were higher than that in March and May. T-N was decreased from March to October. $NH_4-N$ and $NO_3-N$ in the soil of both conventional and organic paddy were higher in May than other seasons. T-P concentration was found to be highest in August, but $P_2O_5$ concentration was lowest in August. It can be concluded that seasonal variation should be considered for analysis and comparison of soil environmental properties.

• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.2
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• pp.120-128
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• 2016

This paper describes power generation from sea waves by using linear permanent magnet generator. A buoy is placed on the ocean surface and connected to the generator. The wave energy is carried out from the movement of a buoy. An electrical conversion system is needed between the generator and the grid. For an analysis of the power system, the modeling of the linear generator and converter system was proceeded. This paper proposes vector control method for wave power generation system using linear generator. In order to verify the proposed method, simulation and experiment performed and the results support the validity of the control scheme.

• Journal of Biomedical Engineering Research
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• v.23 no.2
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• pp.109-117
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• 2002

Electrogastrography(EGG) is a noninvasive method for measuring gastric electrical activity on the abdomen resulting from gastric muscle. EGG signals have a very low frequency range (0.0083 ~0.15 Hz) and extremely low amplitude(10~100 uV). Consequently, EGG signal is easily influenced by other noises. Both finite impulse response(FIR) and infinite impulse response (IIR) filters need high orders or have phase distortions for passing very narrow bandwidth of the EGG signal. In this study, we decomposed EGG signals using a wavelet multiresolution method with Daubechies mother wavelet. The EGG signals were decomposed to seven levels. We reconstructed signal by summing the decomposed signals from level four to seven. To evaluate the performance of the wavelet multiresolution filter(WMF) with simulated EGG signal using two kinds of FIR and four kinds of IIR filters., we used two indices; signal to noise ratio(SNR) and reconstruction squared error(RSE). The SNR of WMF had 9.5, 6.9, and 4.7 dB bigger than that of the other filters at different noise levels, respectively. Also, The RSE of WMF had $1.22{\times}10^6, 1.16{\times}10^6, 1.02{\times}10^6$ smaller than that of the other filters at different noise levels, respectively. The WMF performed better in the SNR and RSE than two kinds of FIR and four kinds of IIR filters.