• Journal of Microbiology and Biotechnology
• /
• v.24 no.8
• /
• pp.1133-1142
• /
• 2014

Interleukin-32 (IL-32) is a cytokine and inducer of various proinflammatory cytokines such as $TNF{\alpha}$, IL-$1{\beta}$, and IL-6 as well as chemokines. There are five splicing variants (${\alpha}$, ${\beta}$, ${\gamma}$, ${\delta}$, and ${\varepsilon}$) and IL-$32{\gamma}$ is the most active isoform. We generated human IL-$32{\gamma}$ transgenic (IL-$32{\gamma}$ TG) mice to express high level of IL-$32{\gamma}$ in various tissues, including immune cells. The pathology of sepsis is based on the systemic inflammatory response that is characterized by upregulating inflammatory cytokines in whole body, particularly in response to gram-negative bacteria. We investigated the role of IL-$32{\gamma}$ in a mouse model of experimental sepsis by using lipopolysaccharides (LPS). We found that IL-$32{\gamma}TG$ mice resisted LPS-induced lethal endotoxemia. IL-$32{\gamma}$ reduced systemic cytokines release after LPS administration but not the local immune response. IL-$32{\gamma}TG$ increased neutrophil influx into the initial foci of the primary injected site, and prolonged local cytokines and chemokines production. These results suggest that neutrophil recruitment in IL-$32{\gamma}TG$ occurred as a result of the local induction of chemokines but not the systemic inflammatory cytokine circulation. Together, our results suggest that IL-$32{\gamma}$ enhances an innate immune response against local infection but inhibits the spread of immune responses, leading to systemic immune disorder.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.3 no.3
• /
• pp.1-7
• /
• 1983

In spite of extensive knowledge of the surface chemistry and the transport mechanism in filtration systems, there is still insufficient understanding of the physical characteristics of suspensions and the system components. Because of this, no filtration mechanisms are mathematically generalized to the full extent. The purpose of this paper is to propose experimental equations for the filtration process. using the tracer study in filter layer. Some of results are as follows. (1) The Volume of the specific deposit (${\sigma}$) in filtration was directly measurable using the tracer study without interrupting the filtration. (2) It was also confirmed that the head loss in filtration was greatly in fluenced by the micro-air babbles. (3) The correction coefficient(f) was introduced into the Kozeny-Carman equation in order to apply it for the clogging filter media. The coefficient(f) was experimentally obtained. The total head loss of the filter media is given by next equation. $${\frac{h}{h_0}}={\frac{1}{L}}{\int}^{z=L}_{z=0}f({\sigma})g({\varepsilon}_0,{\sigma})dz$$ $$f=aexp(-b{\sigma})$$ The above equation was applicable without regard to the variation of the suspension concentration, the filter medium diameter, the filter depth, the filtration velocity, and the amount of aluminum in all continuous filtration experiments. (4) The total head loss was graphically generalized assuming mathematical filtration models I II (see fig. 7,8) (5) The total head loss was obtained from the filtration model in the field filtration conditions. (see fig. 9,10)

• Wind and Structures
• /
• v.20 no.4
• /
• pp.549-564
• /
• 2015

The three-dimensional unsteady incompressible Reynolds-averaged Navier-Stokes equations and k-${\varepsilon}$ double equations turbulent model were used to investigate the effect on the measurements of anemometers due to a passing high-speed train. Sliding mesh technology in Fluent was utilized to treat the moving boundary problem. The high-speed train considered in this paper was with bogies and inter-carriage gaps. Combined with the results of the wind tunnel test in a published paper, the accuracy of the present numerical method was validated to be used for further study. In addition, the difference of slipstream between three-car and eight-car grouping models was analyzed, and a series of numerical simulations were carried out to study the influences of the anemometer heights, the train speeds, the crosswind speeds and the directions of the induced slipstream on the measurements of the anemometers. The results show that the influence factors of the train-induced slipstream are the passing head car and tail car. Using the three-car grouping model to analyze the train-induced flow is reasonable. The maxima of horizontal slipstream velocity tend to reduce as the height of the anemometer increases. With the train speed increasing, the relationship between $V_{train}$ and $V_{induced\;slipstream}$ can be expressed with linear increment. In the absence of natural wind conditions, from the head car arriving to the tail car leaving, the induced wind direction changes about $330^{\circ}$, while under the crosswind condition the wind direction fluctuates around $-90^{\circ}$. With the crosswind speed increasing, the peaks of $V_X,{\mid}V_{XY}-V_{wind}{\mid}$ of the head car and that of $V_X$ of the tail car tend to enlarge. Thus, when anemometers are installed along high-speed railways, it is important to study the effect on the measurements of anemometers due to the train-induced slipstream.

• Proceedings of the Acoustical Society of Korea Conference
• /
• spring
• /
• pp.469-474
• /
• 2002

In recent years, modularization of engine parts has increased the application of plastic products in air intake systems. Plastic intake manifolds provide many advantages including reduced weight, contracted cost, and lower intake air temperatures. These manifolds, however, have some weakness when compared with customary aluminium intake manifolds, in that they have low sound transmission loss because of their lower material density. This low transmission loss of plastic intake manifolds causes several problems related to flow noise, especially when the throttle is opened quickly. The physical processes, responsible for this flow noise, include turbulent fluid motion and relative motion of the throttle to the airflow. The former is generated by high-speed airflow in the splits between the throttle valve and the inner-surface of the throttle body and surge-tank, which can be categorized into the quadrupole source. The latter induces the unsteady force on the flow, which can be classified into the dipole source. In this paper, the mechanism of noise generation from the turbulence is only investigated as a preliminary study. Stochastic noise source synthesis method is adopted for the analysis of turbulence-induced, i.e. quadrupole noise by throttle at quick opening state. The method consists of three procedures. The first step corresponds to the preliminary time-averaged Navier-Stokes computation with a $k-\varepsilon$ turbulence model providing mean flow field characteristics. The second step is the synthesis of time-dependent turbulent velocity field associated with quadrupole noise sources. The final step is devoted to the determination of acoustic source terms associated with turbulent velocity. For the first step, we used market available analysis tools such as STAR-CD, the trade names of fluid analysis tools available on the market. The steady state flows at three open angle of throttle valve, i.e. 20, 35 and 60 degree, are numerically analyzed. Then, time-dependent turbulent velocity fields are produced by using the stochastic model and the flow analysis results. Using this turbulent velocity field, the turbulence-originated noise sources, i.e. the self-noise and shear-noise sources are synthesized. Based on these numerical results, it is found that the origin of the turbulent flow and noise might be attributed to the process of formulation and the interaction of two vortex lines formed in the downstream of the throttle valve. These vortex lines are produced by the non-uniform splits between the throttle valve and inner cylinder surface. Based on the analysis, we present the low-noise design of the inner geometry of throttle body.

• The Journal of the Acoustical Society of Korea
• /
• v.38 no.2
• /
• pp.184-192
• /
• 2019

Compared to 31-mode rings, 33-mode rings are highly utilized as wide bandwidth underwater acoustic transducers because the electro-mechanical coupling and piezoelectric constant d are high. On the other hand, the 31-mode ring is an axial symmetry structure, so it is possible to model it as a simple two-dimensional asymmetrical model for numerical analysis, but the 33-mode ring requires a three-dimensional numerical analysis. That is, a lot of computing resources and computation time are required. In this study, the effective material properties of an equivalent 31-mode ring were derived to simulate the electro-mechano-acoustical responses of the 33-mode ring transducer. Using the effective material properties derived from this study, a numerical analysis of rings in vacuum, air backed rings in water, and FFR (Free Flooded Ring) transducers were performed to compare the responses of 33-mode rings.

• BMB Reports
• /
• v.55 no.6
• /
• pp.275-280
• /
• 2022

The treatment of atopic dermatitis (AD) is challenging due to its complex etiology. From epidermal disruption to chronic inflammation, various cells and inflammatory pathways contribute to the progression of AD. As with immunosuppressants, general inhibition of inflammatory pathways can be effective, but this approach is not suitable for long-term treatment due to its side effects. This study aimed to identify a plant extract (PE) with anti-inflammatory effects on multiple cell types involved in AD development and provide relevant mechanistic evidence. Degranulation was measured in RBL-2H3 cells to screen 30 PEs native to South Korea. To investigate the anti-inflammatory effects of Parasenecio auriculatus var. matsumurana Nakai extract (PAE) in AD, production of cytokines and nitric oxide, activation status of FcεRI and TLR4 signaling, cell-cell junction, and cell viability were evaluated using qRT-PCR, western blotting, confocal microscopy, Griess system, and an MTT assay in RBL-2H3, HEK293, RAW264.7, and HaCaT cells. For in vivo experiments, a DNCBinduced AD mouse model was constructed, and hematoxylin and eosin, periodic acid-Schiff, toluidine blue, and F4/80-staining were performed. The chemical constituents of PAE were analyzed by HPLC-MS. By measuring the anti-degranulation effects of 30 PEs in RBL-2H3 cells, we found that Paeonia lactiflora Pall., PA, and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. show an inhibitory activity of more than 50%. Of these, PAE most dramatically and consistently suppressed cytokine expression, including IL-4, IL-9, IL-13, and TNF-α. PAE potently inhibited FcεRI signaling, which mechanistically supports its basophil-stabilizing effects, and PAE downregulated cytokines and NO production in macrophages via perturbation of toll-like receptor signaling. Moreover, PAE suppressed cytokine production in keratinocytes and upregulated the expression of tight junction molecules ZO-1 and occludin. In a DNCB-induced AD mouse model, the topical application of PAE significantly improved atopic index scores, immune cell infiltration, cytokine expression, abnormal activation of signaling molecules in FcεRI and TLR signaling, and damaged skin structure compared with dexamethasone. The anti-inflammatory effect of PAE was mainly due to integerrimine. Our findings suggest that PAE could potently inhibit multi-inflammatory cells involved in AD development, synergistically block the propagation of inflammatory responses, and thus alleviate AD symptoms.

• Journal of Korea Technology Innovation Society
• /
• v.17 no.2
• /
• pp.380-420
• /
• 2014

We increasingly see the importance of employees acquiring enough expert capability or innovation capability to prepare for ever growing uncertainties in their operation domains. However, despite the above circumstances, there have not been an enough number of researches on how operational input components for employees' innovation outcome, innovation activities such as acquisition, exercise and promotion effort of employee's innovation capability, and their resulting innovation outcome interact with each other. This trend is believed to have been resulted because most of the current researches on innovation focus on the units of country, industry and corporate entity levels but not on an individual corporation's innovation input components, innovation outcome and innovation activities themselves. Therefore, this study intends to avoid the currently prevalent study frames and views on innovation and focus more on the strategic policies required for the enhancement of an organization's innovation capabilities by quantitatively analyzing employees' innovation outcomes and their most suggested relevant innovation activities. The research model that this study deploys offers both linear and structural model on the trio of learning, innovation capability and innovation outcome, and then suggests the 4 relevant hypotheses which are quantitatively tested and analyzed as follows: Hypothesis 1] The different levels of innovation capability produce different innovation outcomes (accepted, p-value = 0.000<0.05). Hypothesis 2] The different amounts of learning time produce different innovation capabilities (rejected, p-value = 0.199, 0.220>0.05). Hypothesis 3] The different amounts of learning time produce different innovation outcomes. (accepted, p-value = 0.000<0.05). Hypothesis 4] the innovation capability acts as a significant parameter in the relationship of the amount of learning time and innovation outcome (structural modeling test). This structural model after the t-tests on Hypotheses 1 through 4 proves that irregular on-the-job training and e-learning directly affects the learning time factor while job experience level, employment period and capability level measurement also directly impacts on the innovation capability factor. Also this hypothesis gets further supported by the fact that the patent time absolutely and directly affects the innovation capability factor rather than the learning time factor. Through the 4 hypotheses, this study proposes as measures to maximize an organization's innovation outcome. firstly, frequent irregular on-the-job training that is based on an e-learning system, secondly, efficient innovation management of employment period, job skill levels, etc through active sponsorship and energization community of practice (CoP) as a form of irregular learning, and thirdly a model of Yί=f(e, i, s, t, w)+${\varepsilon}$ as an innovation outcome function that is soundly based on a smart system of capability level measurement. The innovation outcome function is what this study considers the most appropriate and important reference model.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.23 no.2
• /
• pp.251-257
• /
• 2012

Human body communication means transmitting and receiving data through human body medium or through free space along with the human body skin. Electric field distribution around the human body between the transmitter and the receiver were calculated at five different frequencies with 5 MHz interval between 10 MHz and 30 MHz. Commercial electromagnetic simulation tool was used for the calculation of E-field distributions applying the Korean standard male model including 29 different kinds of human tissues. After calculating specific absorption rate(SAR) values on back of the hand, it was compared with International Commission on Non-Ionizing Radiation Protection(ICNIRP) human protection guideline. While conductivities(${\sigma}$) and relative permittivities(${\varepsilon}_r$) of the human tissues for each frequency were input as the analyzing parameters, electric field intensities near both hands were integrated along the integral line between the nearby electrodes for the calculation of the transmitting and receiving voltages whose ratio was defined as channel loss. The calculated channel losses were about ($75{\pm}1$) dB and showed nearly flat response all through the evaluated frequencies.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.18 no.1
• /
• pp.116-134
• /
• 2005

Although the parallel prescription of Sopoongsangagambang (SG) administration along with external treatment such as spraying or ointment application on the skin is clinically used for the treatment of atopic dermatitis (AD), molecular mechanism underlying its effectiveness is unknown. Thus in the present study, diverse immune responses in terms of chemical mediators related to AD were investigated using an atopic mouse model NC/Nga after SG administration and external treatment (ET), and major findings are summarized as follows. 1. The clinical severities in 16 and 20 week old NC/Nga mice with SG and ET treatment were decreased to 72.2% and 62.3% respectively compared to the control NC/Nga mice with no drug treatment. 2. IgE, IL-4, IL-5, IL-6, IL-13, IgM, IgG1 and IgG2a levels in the serum of SG and ET treated NC/Nga mouse group were significantly decreased compared to the untreated control mice. In contrast, $IFN-{\gamma}$ showed a significant increase in the experimental group compared to the untreated control group. 3. The spleen weight of SG and ET treated NC/Nga mice was significantly decreased compared to the untreated control group. 4. The B/T ratio in the lymph node of SG and ET treated NC/Nga mice was increased compared to the untreated control group. $CD4^+\;and\;CD8^+$ cell numbers in the lymph node of SG and ET treated NC/Nga mice were significantly increased compared to the untreated control group, but $CD69^+\;and\;CD11a^+$ cells were significantly decreased. 5. mRNA expression levels of IL-4, IL-5, and CCR3 in the skin tissues of SG and ET treated NC/Nga mice were significantly decreased, and expression levels of IL-6, IL-13, $CD69^+/CD3{\varepsilon}^+\;and\;CD19^+/CD44^+$ in the skin tissues of SG and ET treated NC/Mga mice were significantly decreased compared to the untreated control group. $IFN-{\gamma}$ mRNA expression levels were increased compared to the untreated control group. 6. Histological observation of the ear and neck skin tissues showed that the extents of inflammation and infiltrated immune cells in the epidermis and dermis of SG and ET treated NC/Nga mice were highly reduced compared to the untreated control group. 7. Lymphokine assay showed a significant decrease in IL-4 levels in SG and ET treated NC/Nga mice compared to the untreated control group, but the levels of $IFN-{\gamma}$ secretion were significantly increased drug treated NC/Nga mice.

• Journal of Theoretical and Applied Mechanics
• /
• v.1 no.1
• /
• pp.69-88
• /
• 1995

The paper attempts to estimate the incubation time of a cavity in the interface between a power law creep particle and an elastic matrix subjected to a uniaxial stress. Since the power law creep particle is time dependent, the stresses in the interface relax. Through previous stress analysis related to the present physical model, the relaxation time is defined by ${\alpha}$2 which satisfies the equation $\Gamma$0 ｜1+${\alpha}$2k｜m=1-${\alpha}$2 [19]. $\Gamma$0=2(1/√3)1+m($\sigma$$\infty$/2${\mu}$)m($\sigma$0/$\sigma$$\infty$tm) where $\sigma$$\infty$ is an applied stress, ${\mu}$ is a shear modulus of a matrix, $\sigma$$\infty$ is a material constant of a power law particle, $\sigma$=$\sigma$0 $\varepsilon$ and t elapsed time. the volume free energy associated with Helmholtz free energy includes strain energies associated with Helmholtz free energy includes strain energies caused by applied stress anddislocations piled up in interface (DPI). The energy due to DPI is found by modifying the results of Dundurs and Mura[20]. The volume free energies caused by both applied stress and DPI are a function of the cavity size(${\gamma}$) and elapsed time(t) and arise from stress relaxation in the interface. Critical radius ${\gamma}$ and incubation time t to maximize Helmholtz free energy is found in present analysis. Also, kinetics of cavity fourmation are investigated using the results obtained by Riede[16]. The incubation time is defied in the analysis as the time required to satisfy both the thermodynamic and kinetic conditions. Through the analysis it is found that [1] strain energy caused by the applied stress does not contribute significantly to the thermodynamic and kinetic conditions of a cavity formation, 2) in order to satisfy both thermodynamic and kinetic conditions, critical radius ${\gamma}$ decreases or holds constant with increase of time until the kinetic condition(eq.40) is satisfied. Therefore the cavity may not grow right after it is formed, as postulated by Harris[11], and Ishida and Mclean[12], 3) the effects of strain rate exponent (m), material constant $\sigma$0, volume fraction of the particle to matrix(f) and particle size on the incubation time are estimated using material constants of the copper as matrix.