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Correlation Between Sorangium cellulosum Subgroups and Their Potential for Secondary Metabolite Production

  • Lee, Chayul;An, Dongju;Lee, Hanbit;Cho, Kyungyun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.3
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    • pp.297-303
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    • 2013
  • Phylogenetic analysis of the groEL1 and xynB1 gene sequences from Sorangium cellulosum strains isolated in Korea previously revealed the existence of at least 5 subgroups (A-E). In the present study, we used sequence analysis of polymerase chain reaction-amplified biosynthetic genes of strains from the 5 subgroups to indicate correlations between S. cellulosum subgroups and their secondary metabolic gene categories. We detected putative biosynthetic genes for disorazol, epothilone, ambruticin, and soraphen in group A, group C, group D, and group E strains, respectively. With the exception of KYC3204, culture extracts from group A, group B, and group C strains exhibited no noticeable antimicrobial inhibitory activities. By contrast, culture extracts from group D strains inhibited the growth of Candida albicans, whereas culture extracts from group E strains inhibited the growth of C. albicans and Staphylococcus aureus. High performance liquid chromatography analysis of the culture extracts from the strains of each subgroup revealed unique peak patterns. Our findings indicate the existence of at least 5 subgroups of S. cellulosum strains, each of which has the potential to produce a unique set of secondary metabolites.

TASTE COMPOUNDS OF FRESH-WATER FISHES 2. Organic Bases in the Muscle of Wild Common Carp (담수어의 정미성분에 관한 연구 2. 천연산 잉어의 유기염기)

  • YANG Syng-Taek;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.13 no.3
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    • pp.109-113
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    • 1980
  • Organic bases in the dorsal meat of wild common carp, Cyprinus carpio, were analyzed as a part of the studies on the taste compounds of fresh-water fishes. The male and female meat showed no significant differences in contents of total creatinine, betaine, TMA and TMAO. The most abundant basic compound was total creatinine $(ca.\;400mg\%)$. The amount of betaine was poor $(ca.\;66.5mg\%)$ and TMA and TMAO were trace in content $(below\;1mg\%)$.

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Effects of NEES on PARP Expression in the Corpus Striatum in Rats Induced with Transient Global Ischemia

  • Lee, Jung Sook;Song, Young Wha;Kim, Sung Won
    • Journal of International Academy of Physical Therapy Research
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    • v.3 no.2
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    • pp.429-434
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    • 2012
  • Ischemia, the leading cause of strokes, is known to be deeply related to synaptic plasticity and apoptosis in tissue damage due to ischemic conditions or trauma. The purpose of this study was to research the effects of NEES(needle electrode electrical stimulation) in brain cells of ischemia-induced rat, more specifically the effects of Poly[ADP-ribose] polymerase(PARP) on the corpus striatum. Ischemia was induced in SD mice by occluding the common carotid artery for 5 minutes, after which blood was re-perfused. NEES was applied to acupuncture points, at 12, 24, and 48 hours post-ischemia on the joksamri, and at 24 hours post-ischemia on the hapgok. Protein expression was investigated through PARP antibody immuno-reactive cells in the cerebral nerve cells and western blotting. The number of PARP reactive cells in the corpus striatum 24 hours post-ischemia was significantly(p<.05) smaller in the NEES group compared to the global ischemia(GI) group. PARP expression 24 hours post-ischemia was very significantly smaller in the NEES group compared to the GI group. Results show that ischemia increases PARP expression and stimulates necrosis, making it a leading cause of death of nerve cells. NEES can decrease protein expression related to cell death, protecting neurons and preventing neuronal apoptosis.

Embodiment of supersonic waves multi curer for Fracture patient's radish stimulation rehabilitation promotion (Fracture 환자의 무자극 재활촉진을 위한 초음파 다층치료기의 구현)

  • Kim, Whi-Young
    • Proceedings of the IEEK Conference
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    • 2006.06a
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    • pp.857-858
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    • 2006
  • Estrangement hierarchical by bipolarization is deepened and time space that social welfare by graying corresponds great so. Specially, is real condition that indifference by patient's increase which is solitary life string is come to involve by social problem. Together, Jaetaek bone fracture patient's ratio is zooming. Domestic BT technology, medical treatment solution technology offer more important role than role assistance enemy of modern technology and utilize by creative technology can . Specially, if apply supersonic waves in bone fracture treatment, there is treatise data that can reduce bone fracture treatment period of bone that bone does not stick well about 40%. Supersonic waves operation frequency used on both end because do 1m Hz, 1.3mHz, supersonic waves origination that have 1.5mHz's Piezo-ceramic crystal tranducer material each 4 premature senilitys in this research, and outside diameter according to impedance and Phase d used Gakgak4mm, 5.4mm, Dukke0.5mm, transformer deuce of length 70mm. Manufactured, and investigated supersonic waves distribution chart by capacity 50m W. Supersonic waves used by diagnosis mainly but is seen to become convenient medical treatment mounting in bone fracture patient's treatment if supplement clinically. If supplement system furthermore, is going to apply to osteoporosis patient, and this research tried to design poetic theme width directly and study rain standardization special quality and approach basic form because do modelling.

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Embodiment of living body measure system modelling for rehalibitation treatment of positive simulation for HRV algorithm analysis interface of Mobile base (모바일기반의 HRV 인터페이스에 처리에 대한 생체계측 시스템 모델링의 구현)

  • Kim, Whi-Young
    • Proceedings of the IEEK Conference
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    • 2006.06a
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    • pp.1013-1014
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    • 2006
  • Mobilecomputer offers more fundamental role than role assistance enemy of modern technology equipment and new Information Technology can reconsider, and reconstruct creatively accuracy of physiological concept. That military register symptoms are developed of disease, before far before rehalibitation, offer possibility that can intervene in process that motive change of military register symptoms after rehalibitation. But, that many parameters become analysis target and mathematical settlement and equalization system of neted data of that is huge, same time collection of all datas can lift difficulty etc.. These main weakness puts in structural relation between elements that compose system. Therefore, dynamics research that time urea of systematic adjustment has selected method code Tuesday nerve dynamics enemy who groping of approach that become analysis point is proper and do with recycling bioelectricity signal. Nature model of do living body signal digital analysis chapter as research result could be developed and scientific foundation groping could apply HSS (Hardware-software system) by rehalibitation purpose. Special quality that is done radish form Tuesday of bioelectricity signal formation furthermore studied, and by the result, fundamental process of bodysignal in do structure circuit form of analog - digital water supply height modelling do can

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Real time control special quality research for $CO_2$ laser's output change rate stability for accumulation style surgical operation rehabilitation of ventriculus that use Photosensor (Photosensor를 이용한 재활 치료형을 위한 $CO_2$ laser 의 출력변동율 안정을 위한 실시간 제어특성 연구)

  • Kim, Whi-Young
    • Proceedings of the IEEK Conference
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    • 2006.06a
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    • pp.1015-1016
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    • 2006
  • The important parameters deciding the fluctuation of Accumulation style surgical operation of ventriculus laser beam are smoothing capacitor, frequency and he characteristics of laser resonator. In this thesis, we control the fluctuation of medical $CO_2C$ laser in realtime by changing Duty-Ratio of IGBT and switching frequency with fixed the smoothing capacitor to improve the fluctuation of laser beam. We detect the light on laser resonator using a CdS photo sensor to improve ripple factor of laser beam and feedback fluctuated signals refined by a band pass filter into the control circuit to stabilize fluctuation actively. There is much to be desired in the realtime controlling technique of the light on Accumulation style surgical operation of ventriculus laser discharge tube in electrical signal. We propose switching control technique with microprocessor and photo sensing technique by controlling switch devices optimum operation and feedback signals detected by a photo sensor into the laser power supply in order to improve ripple factor of the $CO_2$ laser beam.

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A Study on Numerical Simulation for Predicting of Unmanned Undersea Vehicle's Manoeuvrability (수중운동체의 조종성능 예측을 위한 수치시뮬레이션에 대한 연구)

  • Bae, Jun-Young
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.83-85
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    • 2015
  • The Purpose of this paper was to carry out basic study on development of real-time submarine-hnadling simulator. The author adopt the Unmanned Undersea vehicle(UUV), which has taken the shape of manta[1]. They call here it Unmanned Undersea Vehicle(UUV). UUV is based on the same design concept as UUV called Manta Test Vehicle, which was originally built by the Naval Undersea Warfare Center, USA[1]. The present study deals with prediction of manoeuvring motion of UUV at general drift angles and large drift angles. The dynamic mathematical model with six degrees of freedom is revised and supplemented in order to describe accurately motion of UUV. The hydrodynamic derivatives related to motion are obtained from previous work[2].

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A Study of Heart Murmur Quantification (심잡음 정량화에 관한 연구)

  • Eum, Sang-hee
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.05a
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    • pp.252-255
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    • 2016
  • The objective of this paper is to find an easier and non-invasive a way of diagnosing heart diseases based on the heart sound, rigidly heart murmurs, recordings from subjects. Although most of the heart sounds can be easily heard, analysis of the findings by auscultation strongly depends on skills and experience of the physician. Therefore, the heart murmur is require quantitative analysis for automatic diagnosis equipment. For a good sound analysis, the noisy component ware filtered. This can be done using Wiener filter. Once the signal is filtered, it can be segmented into its basic components by signal energy using FFT. After segment the heart sound signal, the relative positions of the different heart sound components will be identified and will be used for quantification purposes. We are using murmur energy ratio. The experimental results are fairly good in relation to automatic diagnosis.

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A Design of Gateway for Industrial Communication (산업용 통신 게이트웨이 설계)

  • Eum, Sang-hee;Lee, Byong-hoon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.05a
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    • pp.281-283
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    • 2016
  • Recently, many industrial instruments face the problem of protocol compatibility with the external monitoring and control system. This paper is prepared in the main control board to support the industrial communication protocol conversion, control, and monitoring. The industrial communication gateway module is also designed to ensure that the protocol conversion of CAN bus and Ethernet. The main board processor is used the Atmega2560, and placed 4ea RS485 serial slots for sub-board. One of them is used for communication CAN bus and Ethernet. It provides analog and digital I / O through each of the slots is used for control and monitoring.

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A Development of Support Smart Application for Emergency Rescue Activities (스마트 구급 활동 지원 앱 개발)

  • Eum, Sang-hee;Kim, Gi-Ryon;Kim, Gwang-nyeon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.05a
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    • pp.238-240
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    • 2017
  • In the recent years, An attempts have been made to convergence medical technology and IT technology to overcome the limitations of temporal, spatial, and medical technology applications. In this study, we developed an application s/w to support emergency services for emergency medical services. The developed smart application for emergency rescue activities support can easily identify emergency situation and emergency diary. Then, it can be transmitted to a remote medical guidance doctor so that emergency treatment and hospital response can be performed quickly.

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