• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.183-188
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• 2009

Seven isolates showing strong proteolytic activity, KYC 1028, 1100, 1134, 1139, 1151, 1159, and 1182, were collected. Out of them, the broth of KYC 1134 and KYC 1139 showed the high proteolytic activity measured by azocazein. To determine 16S rDNA sequences for identification, 16S rDNA of seven isolates were amplified and compared with the 16S rDNA sequences of other myxobacteria at NCBI. It is evident from the phylo-genetic tree that the isolates belong to the genus Myxococcus. Sharing high percentage similarity values with myxobacteria, the 16S rDNA sequences were involved in two species, Myxococcus macrospores and M. Fulvus. Biochemical characteristics of KYC 1134 broth, which showed the highest proteolytic activity, showed increased activity 8 times to seven days after culture, and protein production were increased gradually and stopped at five days. The broth had optimal temperature at $60^{\circ}C$ for proteolytic activity, and stability of pH was ranged from pH 5 to 10, at $50^{\circ}C$ and 60, respectively. To classify proteases being in the broth, ten inhibitors were determined and only bestatin showed 27% inhibition effect. The inhibition result demonstrates that the broth contains kinds of amino peptidases and other exopeptidases.

• The Korean Journal of Pesticide Science
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• v.2 no.2
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• pp.39-44
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• 1998

Three pesticides for paddy rice, iprobenfos, isoprothiolane, and diazinon were examined on some environmental factors, their hydrolysis, microbial degradation, and photolysis in aqueous systems. Iprobenfos was mainly degraded by microorganisms and its half-life was 5.7 days at $28^{\circ}C$ in aqueous systems. Hydrolysis of iprobenfos was accelerated by the higher temperature, but its photodegradation was accelerated by the lower pH. Isoprothiolane was rapidly decomposed by two factors, microorganisms and sunlight. The half-life of isoprothiolane by sunlight was 91 days at pH 9.0, while it was 13 days at pH 4.0 and 16 days at pH 7.2. However, it was shortened under low pH condition. In aqueous system, diazinon was degraded by all of three factors and its degradation rate was remarkably accelerated by acidic solution. Main degradation factors of iprobenfos, isoprothiolane, and diazinon in the aqueous system were investigated by microbial degradation, photolysis, and hydrolysis, respectively. The strains of microbial degradation for iprobenfos, isoprothiolane, and diazinon in the aqueous environment were identified as Pseudomonas putida, Alcaligenes xylosoxydans ss, Klebsiella planticola/ornithinllytica, respectively. The similarity rates of identity were $54.8{\sim}86.2%$ with biolog-system.

• The KIPS Transactions:PartB
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• v.12B no.1 s.97
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• pp.47-56
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• 2005

A moving object has a various features that its spatial location, shape, and size are changed as time goes. In addition, the moving object has both temporal feature and spatial feature. It is one of the highly interested feature information in video data. In this paper, we propose an efficient content-based multimedia information retrieval system, so tailed ECoMOT which enables user to retrieve video data by using a trajectory information of moving objects in video data. The ECoMOT includes several novel techniques to achieve content-based retrieval using moving objects' trajectories : (1) Muitiple trajectory modeling technique to model the multiple trajectories composed of several moving objects; (2) Multiple similar trajectory retrieval technique to retrieve more similar trajectories by measuring similarity between a given two trajectories composed of several moving objects; (3) Superimposed signature-based trajectory indexing technique to effectively search corresponding trajectories from a large trajectory databases; (4) convenient trajectory extraction, query generation, and retrieval interface based on graphic user interface

• Journal of KIISE:Software and Applications
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• v.35 no.11
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• pp.671-680
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• 2008

Motion capture devices have been utilized in producing several contents, such as movies and video games. However, since motion capture devices are expensive and inconvenient to use, motions segmented from captured data was recycled and synthesized to utilize it in another contents, but the motions were generally segmented by contents producers in manual. Therefore, automatic motion segmentation is recently getting a lot of attentions. Previous approaches are divided into on-line and off-line, where ow line approaches segment motions based on similarities between neighboring frames and off-line approaches segment motions by capturing the global characteristics in feature space. In this paper, we propose a graph-based high-level motion segmentation method. Since high-level motions consist of repeated frames within temporal distances, we consider similarities between neighboring frames as well as all similarities among all frames within the temporal distance. This is achieved by constructing a graph, where each vertex represents a frame and the edges between the frames are weighted by their similarity. Then, normalized cuts algorithm is used to partition the constructed graph into several sub-graphs by globally finding minimum cuts. In the experiments, the results using the proposed method showed better performance than PCA-based method in on-line and GMM-based method in off-line, as the proposed method globally segment motions from the graph constructed based similarities between neighboring frames as well as similarities among all frames within temporal distances.

• Korean Journal of Environment and Ecology
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• v.2 no.1
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• pp.37-49
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• 1988

This study was conducted to investigate the user's effects on the avifauna in Chiak Mountain National Park. The survey was carried over 5 districts which were 3 main trail (valley) by line transect method from May to October 1988. The observed birds were 44 species and 613 individuals. these consist of 22 species for residents, 13 species for summer visitor, 5 species for winter visitor and 4 species for passage migrant. The average density of birds was 2.96 ea/ha, and main dominant species were Panus minar, Paradoxomis webbiana fulvicanda, Panus palustris hellmayri and Parus ater amurensis in order. The observed frequency of wild-birds was 6.77 times/km in average. No. of species, species diversities and observed frequency were lower in main trail of high user's density than sub trail and closed trail, especially in summer. But similarity indices were affected by inhabitation environments than user's density. Therefore, from now, in Chiak Mountain National Park management, the enjoyment oriented activities will be regulated and the control of trail use on main trail are necessary during breeding periods for wild birds protection.

• The Journal of the Korean Society for Microbiology
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• v.35 no.1
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• pp.31-40
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• 2000

Bacillus anthracis, the etiological agent of anthrax has been classified into the Bacillus subgroup I with B. cereus, B. mycoides and B. thuringiensis based on morphological and DNA similarity. DNA studies have further indicated that these species have very AT-rich genomes and high homology, indeed it has been proposed that these four sub-species be recognized as members of the one species. Several methods have been developed to obtain good differentiation between these species. However, none of these methods provides the means for an absolutely correct differntiation. The analysis of fatty acid methyl esters (FAMEs) was employed as a quick, simple and reliable method for the identification of 21 B. anthracis strains and closley related strains. The most significant differences were found between B. anthracis and B. anthracis closely related strains in FAMEs profiles. All tested strains of B. anthracis had a branched fatty acid C17:1 Anteiso A, whereas the fraction of unsaturated fatty acid Iso C17:1 w10c was found in B. anthracis closely related strains. By UPGMA clustering analysis of FAMEs profiles, all of the tested strains were classified into two clusters defined at Euclidian distance value of 24.5. The tested strains of B. anthracis were clustered together including Bacillus sp. Kyungjoo 3. However, the isolates of B. anthracis closely related spp. Rho, S10A, 11R1, CAU9910, CAU9911, CAU9912 and CAU9913 were clustered with the other group. On the basis of these results, isolates of B. anthracis Bongchon, Kyungjoo 1, 2 and Bacillus sp. Kyungjoo 3 were reclassified as a B. anthracis. It is concluded that FAMEs analysis provides a sensitive and reliable method for the identification of B. anthracis from closely related taxa.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.9
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• pp.643-651
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• 2020

A capsule train runs inside a sub-vacuum tube and can reach very high speed due to the low air resistance. A capsule train uses a superconducting electrodynamic suspension (SC-EDS) method for levitation, which allows for a large levitation gap and does not require gap control. However, SC-EDS has inherent characteristics such as the large gap variation and a small damping effect in the levitation force, which can degrade the running stability and ride comfort. To overcome this, a stability improvement device should be designed and applied based on dynamic analysis. In this study, a 1/10 small-scale testbed was developed to replicate the dynamic characteristics of a capsule train and investigate the performance of stability improvement devices. The testbed is composed of a 6-degree-of-freedom Stewart platform for the realization of bogie motion, a secondary suspension with a running stabilization device, and a carbody. Based on the dynamic similarity law proposed by Jaschinski, the small-scale testbed was manufactured, and a bogie motion algorithm was applied with the consideration of guideway irregularity and levitation stiffness. The experimental results from the testbed were compared with simulation results to investigate the performance of the testbed.

• Journal of Korean Society of Forest Science
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• v.62 no.1
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• pp.43-52
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• 1983

The purpose of this study was to measure the changes in soil properties and forest vegetation after surface fire, which had occured on June 5, 1983 in Mt. Gwanag, Kyunggi-do. Moisture content, organic matter and acidity of soil increased just after the fire and then dropped down up to those of unfired areas as time goes. Also total nitrogen, available phosphorous exchangeable base had the similar trend to moisture, organic matter and acidity. Most of the exchangeable bases in surface soil except for sodium were higher than those in sub-soil. No changes in soil texture by the fire were found. Increasers, decreasers, invaders and neutral species could be classified according to the relative importance value of each species. Species diversity was reduced just after the fire and increased gradually afterward. Diversity in the southeast slope was higher than that in the southwest slope. Due to the fire, evenness of woody plants decreased continuously while that of herbs increased. Species similarity was shown greater at fired areas than at unfired areas.

• Journal of Broadcast Engineering
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• v.22 no.3
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• pp.282-294
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• 2017

In this paper, we propose a novel intelligent headlight control (IHC) system which is durable to various road lights and camera movement caused by vehicle driving. For detecting candidate light blobs, the region of interest (ROI) is decided as front ROI (FROI) and back ROI (BROI) by considering the camera geometry based on perspective range estimation model. Then, light blobs such as headlights, taillights of vehicles, reflection light as well as the surrounding road lighting are segmented using two different adaptive thresholding. From the number of segmented blobs, taillights are first detected using the redness checking and random forest classifier based on Haar-like feature. For the headlight and taillight classification, we use the random forest instead of popular support vector machine or convolutional neural networks for supporting fast learning and testing in real-life applications. Pairing is performed by using the predefined geometric rules, such as vertical coordinate similarity and association check between blobs. The proposed algorithm was successfully applied to various driving sequences in night-time, and the results show that the performance of the proposed algorithms is better than that of recent related works.

• Journal of Life Science
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• v.25 no.5
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• pp.568-576
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• 2015

The marine microalga Pavlova viridis can grow fast and has the ability to accumulate essential nutrients for culturing marine animals, such as EPA and DHA, and it has been used as food for raring larval fish and prawn. The symbiotic relationship between the flagellate microalga Pavlova viridis and its associated bacteria was investigated. An axenic culture of P. viridis was obtained by repeated treatment of the microalga with an antibiotic cocktail. The axenic status was confirmed after sub-culturing three times in a sterile f/2 medium without an antibiotic. The axenic alga was then co-inoculated with five bacteria, arbitrarily designated as I1–I5, isolated from the alga to test the growth promotion of the algae. All bacterial strains promoted the growth of P. viridis, and bacterial isolate I3 was the most effective among the five bacteria tested. The cell number of P. viridis in the co-culture with I3 was significantly higher than that of the control culture. A sequence analysis of the 16S rRNA gene isolated from I3 revealed a 97% nucleotide sequence similarity to that of Citrobacter sp. The growth of strain I3 was also significantly enhanced by co-culturing with P. viridis, indicating a symbiotic relationship between the microalga and its associated bacterium. The association between the microalga and bacterium was confirmed by scanning electron microscopy.