• 제목/요약/키워드: /t/-insertion

검색결과 365건 처리시간 0.027초

Flanking Sequence and Copy-Number Analysis of Transformation Events by Integrating Next-Generation Sequencing Technology with Southern Blot Hybridization

  • Qin, Yang;Woo, Hee-Jong;Shin, Kong-Sik;Lim, Myung-Ho;Cho, Hyun-Suk;Lee, Seong-Kon
    • Plant Breeding and Biotechnology
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    • 제5권4호
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    • pp.269-281
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    • 2017
  • With the continual development of genetically modified (GM) crops, it has become necessary to develop detailed and effective molecular characterization methods to select candidate events from a large pool of transformation events. Relative to traditional molecular analysis methods such as the polymerase chain reaction (PCR) and Southern blot hybridization, next generation sequencing (NGS) technology for whole-genome sequencing of complex crop genomes had proven comparatively useful for in-depth molecular characterization. In this study, four transformation events, including one in Bacillus thuringiensis (Bt)-resistant rice, one in resveratrol-producing rice, and two in beta-carotene-enhanced soybeans, were selected for molecular characterization. To merge NGS analysis and Southern blot-hybridization results, we confirmed the transgene insertion sites, insertion construction, and insertion numbers of these four transformation events. In addition, the read-coverage depth assessed by NGS analysis for inserted genes might provide consistent results in terms of inserted T-DNA numbers in case of complex insertion structures and highly duplicated donor genomes; however, PCR-based methods can produce incorrect conclusions. Our combined method provides an effective and complete analytical approach for whole-genome visual inspection of transformation events that require biosafety assessment.

경계요소법에 의한 방음벽의 성능 평가 (Evaluation of the Performance of the Noise Barrier Using the BEM)

  • 황철호
    • 한국소음진동공학회논문집
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    • 제18권1호
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    • pp.94-100
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    • 2008
  • Noise barriers are being used more often to solve problems of noise pollution from traffic noise. Several types of noise barriers are being installed to increase the cost-effectiveness of noise barrier installation. In this study, the insertion loss is analyzed to evaluate the effectiveness of the noise barrier by using the BEM. In order to check the validity of the BEM, the BEM and Lam's theoretical analysis are compared with measurement, which is performed in the anechoic chamber for the 1/10 scale-down model, and good agreements are obtained. By using the two dimensional boundary element method, the insertion loss is calculated and analyzed for several typical noise barriers such as the vertical barrier, the barrier with an oblique edge on top, the T-shaped barrier and the barrier with interference device on top. With these analyses, it is possible to design more cost-effective noise barriers appropriate for a particular area.

Inheritance Analysis of Giant Embryo Mutation Induced by T-DNA Insertion in Rice

  • Qin, Yang;Kim, Suk-Man;Park, Hee-Yeon;Sohn, Jae-Keun
    • 한국육종학회지
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    • 제41권1호
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    • pp.9-15
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    • 2009
  • Recently, giant embryonic rice and functional rice food are preferred by more consumers, which are attributed to the fact that the embryo has high concentrations of essential amino acids, fatty acids, and vitamins relative to other parts of rice grains. In this report, the heredity and stability of giant embryo mutations in successive generations were analyzed regarding a giant embryonic line, 'P47', induced by T-DNA insertion and a $F_2$ population from a cross between 'P47' and 'Junam'. The mutant lines with increases of 1.5, 1.7 and 1.8 times on embryo length, width and 100-embryo weight to those of the control showed stable inheritance across three generations. The continuous frequency distributions of embryo size in the $F_2$ population showed that the embryo size is a quantitative trait of polygene controlled. In addition, wide range of transgressive segregations of six traits affecting embryo size confirmed exchange of genetic materials and recombination between genes controlling embryo size. Five giant embryo mutant lines selected from the $F_2$ population will be used for artificial selection and improvement of giant embryonic varieties.

Temperature Dependence of Microwave Properties of HTS Multipole Lowpass Filters Consisting of Microstrip Open-Stub Lines

  • Kim, Je-Ha;Han, Seok-Kil;Kang, Kwnag-Yong;Ahn, Dal
    • ETRI Journal
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    • 제19권2호
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    • pp.48-58
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    • 1997
  • Using high quality high-$T_c$ superconducting (HTS) YBCO thin films, we designed and fabricated 3-pole, 5-pole, and 7-pole lowpass filters consisting of microstrip transmission lines and open-stub lines. We measured the microwave response of the filters in the temperature range of T=85 K to 27 K. AtT=30 K, the observed inband insertion loss was 0.14dB and 0.02dB for the 5-pole and 7-pole filters, respectively. The cut-off frequency was 4.7 GHz for the 5-pole filter and 7.9GHz for the 7-pole filter. To the authors' knowledge, the measured inband insertion losses are the best values reported so far for the open-stub line type HTS multipole lowpass filters. The skirt property of the 5-pole filter showed a large improvement over that of the 3-pole filter as predicted from the simulation. We found that, to obtain a stable performance, the HTS multipole lowpass filters should be operated at the temperatures between 60% and 70% of $T_c$.

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V/UHF 대역 SP3T 송수신 스위치 설계 (Design of V/UHF-Band SP3T Transmitting/Receiving Switch)

  • 이병남;박동철
    • 한국군사과학기술학회지
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    • 제11권5호
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    • pp.34-41
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    • 2008
  • This paper describes the design of SP3T PIN diode switch which has a 500W high power handling capability in $20{\sim}400MHz$ frequency range. Design factors were investigated and it was confirmed by simulation that the characteristics of insertion loss, VSWR, and isolation met design goal. Also, the capability to handle 500W high power with very fast switching speed of less than $26{\mu}s$ was confirmed and insertion loss of less than 1dB, VSWR of less than 1.4:1, and isolation of higher than 60dB were obtained by experiments.

A Low Insertion-Loss, High-Isolation Switch Based on Single Pole Double Throw for 2.4GHz BLE Applications

  • Truong, Thi Kim Nga;Lee, Dong-Soo;Lee, Kang-Yoon
    • IEIE Transactions on Smart Processing and Computing
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    • 제5권3호
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    • pp.164-168
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    • 2016
  • A low insertion-loss, high-isolation switch based on single pole double throw (SPDT) for a 2.4GHz Bluetooth low-energy transceiver is presented in this paper. In order to increase isolation, the body floating technique is implemented. Based on characteristics whereby the ratio of the sizes of the shunt and the series transistors significantly affect the performance of the switches, the device sizes are optimized. A simple matching network is also designed to enhance the insertion loss. Thus, the SPDT switch has high isolation and low insertion loss without increasing the complexity of the circuit. The proposed SPDT is designed and simulated in a complementary metal-oxide semiconductor 65nm process. The switch has a $530{\mu}m{\times}270{\mu}m$ area and achieves 0.9dB, 1.78dB insertion loss and 40dB, 41dB isolation of transmission, reception modes, respectively.

방음벽 상단부 형상에 따른 삽입손실 연구 (A Study on the Insertion Loss of Noise Barrier with the Variation of Top Shape)

  • 정성수;김용태;이우섭
    • 한국음향학회지
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    • 제21권7호
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    • pp.624-631
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    • 2002
  • 방음벽의 상단부 형상에 따른 삽입손실을 무향실에서 모형축척 방법으로 측정하고 상호 비교하였다. 높이 0.3m의 수직형 방음벽에 대한 삽입손실과 간단한 형상물을 상단부에 설치한 방음벽의 삽입손실과의 차를 비교하였다. 실험결과 단순히 높이를 증가시키는 경우보다 상단부에 간단한 형상을 설치한 것이 더 효과적이었다. 'T'형, 'Y'형 및 '(equation omitted)'형 방음벽에 대한 삽입손실을 비교한 결과 'T'형과 '(equation omitted)'형 방음벽은 비슷하였으며 'Y'형이 가장 좋은 효과를 나타냈다. 본 연구결과는 Alfredson(PIOC. Inter-Noise 95, p. 381, 1995)등의 결과와는 잘 일치하지만 May (J. Sound Vib. 71, p. 73, 1980) 등의 결과와는 상충된다. 따라서 어떤 형태가 가장 좋은지를 결정하는 것은 어렵다. 왜 이러한 상호 모순되는 결과를 얻게 되는가를 알기 위해 경계요소법을 적용한 결과 각 연구자들마다 다른 방음벽의 높이, 음원과 수음점의 위치, 높이와 거리를 서로 다른 실험조건을 가정했기 때문임을 알 수 있었다.

MRI를 통한 풍부혈(GV16)의 안전 자침 깊이에 대한 연구 (Safe Needling Depth of Pungbu(GV16) with MRI-a Retrospective Study)

  • 양현정;박해인;이광호
    • Journal of Acupuncture Research
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    • 제32권4호
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    • pp.11-16
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    • 2015
  • Objectives : The purpose of this study is to determine the safe needling depth of Pungbu($GV_{16}$) retrospectively by using magnetic resonance imaging (MRI). Methods : We chose 114 Brain or C-spine MRI images from the Sang-Ji hospital picture archiving communication system. We measured the shortest distance from skin to cerebral dura mater passing by posterior edge of the foramen magnum on the sagittal view for the depth of Pungbu. We analyzed the differences between male and female measured values by using a student t-test. Results : The average depth of male insertion was $49.71{\pm}6.32mm$ and the shortest depth of insertion was 36.29 mm. The average depth of female insertion was $39.84{\pm}5.25mm$ and the shortest depth of insertion was 30.02 mm. The results showed a significant difference according to gender (p=0.00). Conclusions : The depth of male insertion is deeper than that of female, and the safe needling depth in the case of males is 36.29-67.35 mm, while the safe needling depth in the of females is 30.02-52.18 mm.

Maxillary sinus haziness and facial swelling following suction drainage in the maxilla after orthognathic surgery

  • Lee, Jung-Soo;Kim, Moon-Key;Kang, Sang-Hoon
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제42권
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    • pp.33.1-33.8
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    • 2020
  • Background: We investigated the efficacy of a maxillary Jackson-Pratt (J-P) suction drain for preventing maxillary sinus hematoma and facial swelling after maxillary Le Fort I osteotomy (LF1). Methods: We retrospectively evaluated 66 patients who underwent LF1 at a single institution. Of these, 41 had a J-P suction tube inserted in the mandible and maxilla (maxillary insertion), and 25 had a J-P drain inserted in the mandible only (no maxillary insertion). Facial CT was obtained before and 4 days after surgery. We compared mean midfacial swelling and maxillary sinus haziness by t test and examined correlations between bleeding amount and body mass index (BMI). Results: For the maxillary-insertion group, the ratio of total maxillary sinus volume to haziness (57.5 ± 24.2%) was significantly lower than in the group without maxillary drain insertion (65.5% ± 20.3; P = .043). This latter group, however, did not have a significantly greater midfacial soft tissue volume (7575 mm3) than the maxillary-insertion group (7250 mm3; P = .728). BMI did not correlate significantly with bleeding amount or facial swelling. Conclusions: Suction drainage in the maxilla reduced maxillary sinus haziness after orthognathic surgery but did not significantly reduce midfacial swelling.

Evaluation of the effect of two different occlusal splints on maximum occlusal force in patients with sleep bruxism: a pilot study

  • Karakis, Duygu;Dogan, Arife;Bek, Bulent
    • The Journal of Advanced Prosthodontics
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    • 제6권2호
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    • pp.103-108
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    • 2014
  • PURPOSE. The occlusal splint has been used for many years as an effective treatment of sleep bruxism. Several methods have been used to evaluate efficiency of the occlusal splints. However, the effect of the occlusal splints on occlusal force has not been clarified sufficiently. The purpose of this study was to evaluate the effect of occlusal splints on maximum occlusal force in patients with sleep bruxism and compare two type of splints that are Bruxogard-soft splint and canine protected hard stabilization splint. MATERIALS AND METHODS. Twelve students with sleep bruxism were participated in the present study. All participants used two different occlusal splints during sleep for 6 weeks. Maximum occlusal force was measured with two miniature strain-gage transducers before, 3 and 6 weeks after insertion of occlusal splints. Clinical examination of temporomandibular disorders was performed for all individuals according to the Craniomandibular Index (CMI) before and 6 weeks after the insertion of splints. The changes in mean occlusal force before, 3 and 6 weeks after insertion of both splints were analysed with paired sample t-test. The Wilcoxon test was used for the comparison of the CMI values before and 6 weeks after the insertion of splints. RESULTS. Participants using stabilization splints showed no statistically significant changes in occlusal force before, 3, and 6 weeks after insertion of splint (P>.05) and participants using Bruxogard-soft splint had statistically significant decreased occlusal force 6 weeks after insertion of splint (P<.05). There was statistically significant improvement in the CMI value of the participants in both of the splint groups (P<.05). CONCLUSION. Participants who used Bruxogard-soft splint showed decreases in occlusal force 6 weeks after insertion of splint. The use of both splints led to a significant reduction in the clinical symptoms.