• Molecules and Cells
• /
• v.27 no.2
• /
• pp.183-190
• /
• 2009

The plasma membrane-localized BRASSINOSTEROID-INSENSITIVE1 (BRI1) and BRI1-ASSOCIATED KINASE1 (BAK1) are a well-known receptor pair involved in brassinosteroids (BR) signaling in Arabidposis. The formation of a receptor complex in response to BRs and the subsequent activation of cytoplasmic domain kinase activity share mechanistic characteristics with animal receptor kinases. Here, we demonstrate that BRI1 and BAK1 are BR-dependently phosphorylated, and that phosphorylated forms of the two proteins persist for different lengths of time. Mutations of either protein abolished phosphorylation of the counterpart protein, implying transphosphorylation of the receptor kinases. To investigate the specific amino acids critical for formation of the receptor complex and activation of BAK1 kinase activity, we expressed several versions of BAK1 in yeast and plants. L32E and L46E substitutions resulted in a loss of binding of BAK1 to BRI1, and threonine T455 was essential for the kinase activity of BAK1 in yeast. Transgenic bri1 mutant plants overexpressing BAK1(L46E) displayed reduced apical dominance and seed development. In addition, transgenic wild type plants overexpressing BAK1(T455A) lost the phosphorylation activity normally exhibited in response to BL, leading to semi-dwarfism. These results suggest that BAK1 is a critical component regulating the duration of BR efficacy, even though it cannot directly bind BRs in plants.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.8 no.2
• /
• pp.288-298
• /
• 1996

The gas-fired absorption refrigeration system to utilize treated sewage is available for environmental protection and energy conservation. Simulation analysis on the double-effect absorption refrigeration cycles with parallel or series flow type has been performed. The working fluid is Lithium Bromide and water solution. The main purpose of this study is evaluating the possibilities of effective utilization of treated sewage as a cooling water for the absorber and condenser. The efficiency of a couple of cycles has been studied and simulation results show that higher coefficient of performance could be obtained for parallel flow type. The other purpose of the present study is to determine the optimum designs and operating conditions based on the operating constraints and the coefficent of performance in the paralledl flow type.

• Journal of the Korean Solar Energy Society
• /
• v.27 no.4
• /
• pp.19-26
• /
• 2007

This paper concerns the study of absorption refrigeration and heat pump cycle to use sewage. Simulation analysis on the double-effect absorption refrigeration cycle with parallel and two-stage heat pump cycle has been performed. The working fluid is Lithium Bromide and water solution. The absorption refrigeration cycle use sewage as a cooling water for the absorber and condenser, and absorption refrigeration cycle does that as a chilled water for the evaporator of the first stage cycle. And the two-stage cycle consists of coupling double-effect with parallel and single effect cycle so that the first stage absorber and condenser produces heating water to evaporate refrigerant in the evaporator of the second stage. The effects of operating variables such as a absorber temperature on the coefficient of performance have been studied for absorption refrigeration and heat pump cycle.