• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.637-643
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• 1995

TR75, a rice (Oryza sativa L. var. Sasanishikj) mutant resistant to 5-methyltryptophan (5MT) was segregated from the progenies of its initial mutant line, TR1. The 5MT resistance of TR75 was inherited in the M$_{8}$ generations as a single dominant nuclear gene, and was also expressed in callus derived from seeds, roots, and anthers as well as in the seedlings. The callus induced from these organs could grow at 50 mg/1 of 5MT, whereas the growth of wild-type callus was completely inhibited even at 25 mg/1. The seedlings of TR75 did not show resistance to L-azetidine-2-carboxylic acid, S-2-aminoethyl-L-cysteine, p-fluoro-DL-phenylalanine. The content of free amino acids in the TR75 homozygous seeds increased approximately 1.5 to 2.0 fold compared to wild-type seeds. Especially, the contents of tryptophan, phenylalanine and aspartic acid were 5.0, 5.3 and 2.7 times higher than those of wild-type seeds, respectively.y.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.348-353
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• 2009

Organic materials reveal the remarkable absorption and high buffer capacity for nutrient. Hence, organic materials need some different nutrient management skill from inorganic one. The objective of this study was to evaluate the effect of EC level of nutrient solution on the yield and quality of cut rose grown in the mixed substrate of coir and perlite. 3 EC levels of nutrient solution was treated, which were 0.7, 1.0, 1.3 times of standard solution (Aichiken solution, Japan) for cut rose hydroponics. EC of the standard solution was changed by season following as 1.4 (Apr.~June), 1.0 (July~Aug.), 1.4 (Sep.~Oct.), and $1.6dS{\cdot}m^{-1}$ (Nov.~Mar.) subsequently. The supply of nutrient solution was controlled by the signal of water potential at -5kPa using frequency domain reflectometry (FDR) sensor. As the results, marketable yield was similar for all treatments until 3rd harvest, but was decreased in high EC level from 4th harvest to 7th harvest as final. 0.7 times of standard solution decreased the ratio of unmarketable rose having short stem below 70cm and increased the ratio of high quality rose having long stem above 91cm. The flower weight and stem diameter of cut rose was higher in the low EC treatment than the others.

• Korean Journal of Animal Reproduction
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• v.27 no.2
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• pp.125-133
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• 2003

The present study was carried out to examine the effect of $\beta$-Mercaptoethanol ($\beta$-ME) on in vitro maturation (IVM) of porcine follicular oocytes and oxygen concentration with $\beta$-ME on in vitro development (IVD) of porcine IVM/IVF embryos. The results were summarized as follows. 1. The rates of nuclear maturation, penetrated oocytes, polyspermic oocytes, pronucleus formation and mean numbers of the penetrated sperms were not significantly different using NCSU-23 maturation media for 0, 25, 50 and 100 $\mu$M $\beta$-ME (P>0.05). 2. The rates of blastocyst formation at day 7 after in vitro fertilization were higher in oocytes matured with 25 $\mu$M $\beta$-ME (25.4$\pm$0.9%) than in those matured with 0 (14.5$\pm$1.6%), 50 (17.3$\pm$1.7%) and 100 $\mu$M (12.4$\pm$1.3%) (P<0.05). However, no differences ware found in total cell numbers of blastocyst among the treatments. 3. The rates of blastocyst formation at day 7 after in vitro fertilization were higher in the NCSU-23 Culture medium With 25 $\mu$M $\beta$-ME (23.6$\pm$2.8%) than in those Cultured With 0 (15.4$\pm$4.4%), 12.5 (17.5$\pm$2.3%) and 50 $\mu$M $\beta$-ME (18.6$\pm$2.1%) Under the 5% and 20% $O_2$ Concentrations (P<0.05). However, no differences was found in total cell numbers of blastocyst among the treatments. These results suggested that the addition of 25 $\mu$M $\beta$-ME in the IVM/IVD media were effective on the porcine embryo production. However, the rates of blastocyst formation and total cell numbers of blastocyst at day 7 of porcine IVM/IVF embryos were not significantly different in the NCSU-23 culture medium under 5% and 20% 02 concentrations.

• Korean Journal of Agricultural Science
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• v.34 no.1
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• pp.19-35
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• 2007

The present study was carried out to examine the effect of EGF and IGF-I in vitro maturation (IVM) of porcine oocytes and development of porcine IVM/IVF embryos. The results were summarized as follows : 1. The rates of nuclear maturation, penetrated oocytes, pronuclear formation, polyspermic oocytes and mean numbers of the penetrated sperm were not different in NCSU-23 maturation medium with 0, 1, 5 and 10 ng/ml EGF and IGF-I (P>0.05). 2. The rates of blastocyst formation at day 7 after in vitro fertilization in 0, 1, 5 and 10 ng/ml EGF groups were $11.2{\pm}1.5%$, $15.0{\pm}8.3%$, $16.8{\pm}2.8%$ and $21.4{\pm}2.0%$, also 0, 1, 5 and 10 ng/ml IGF-I groups were $11.2{\pm}1.5%$, $15.0{\pm}8.3%$, $16.8{\pm}2.8%$ and $21.4{\pm}2.0%$, respectively. In the total cells case, EGF groups were $22.8{\pm}3.7$, $25.7{\pm}5.5$, $26.0{\pm}4.2$ and $35.1{\pm}4.7$, also IGF-I groups were $21.5{\pm}3.7$, $25.2{\pm}2.8$, $26.2{\pm}2.9$ and $33.2{\pm}3.6$, respectively. Both 10 ng/ml EGF group and 10 ng/ml IGF-I group were significantly higher than those of other treatment groups (P<0.05). 3. The rates of blastocyst formation at day 7 in the NCSU23 culture medium of porcine IVF-produced embryos with 0, 1, 5, and 10 ng/ml EGF groups were $14.0{\pm}1.7%$, $16.2{\pm}1.4%$, $16.9{\pm}1.2%$ and $23.1{\pm}1.6%$, also 0, 1, 5, 10 ng/ml IGF-I groups were $13.6{\pm}1.7$, $15.7{\pm}4.5$, $16.0{\pm}0.2$ and $25.0{\pm}0.8$, respectively. And in the total cells case, EGF grups were $21.8{\pm}2.9$, $25.2{\pm}2.8$, $39.7{\pm}2.7$ and $46.2{\pm}3.6$, also IGF-I groups were $20.7{\pm}2.9$, $26.2{\pm}2.9$, $24.6{\pm}2.4$ and $46.1{\pm}3.5$, respectively. Both 10 ng/ml EGF group and 10 ng/ml IGF-I group were significantly higher than those of any other treatment groups (P<0.05). In conclusion, these results suggested that the addition of 10 ng/ml EGF and IGF-I were effective on the blastocyst formation and total cells of blastocysts.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.61 no.4
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• pp.227-234
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• 2016

In this study, we analyzed seed storage proteins in order to investigate the main factors related to the eating quality of japonica and tongil-type rice varieties. Sensory evaluation was performed by a trained panel to assess the appearance (color and glossiness), flavor, taste, stickiness, texture, and overall score of nine japonica and three tongil-type rice cultivars. Moreover, the pattern of variation in rice storage proteins was examined by electrophoresis of protein extracts. The electrophoretic pattern of rice proteins showed 16.4 kDa albumin, 26.4 kDa globulin, 34-39 kDa and 21-22 kDa glutelin, and 14.3 kDa prolamin. In terms of storage protein, the varietal differences between japonica and tongil-type rice were found in albumin, globulin, and the ${\alpha}-1$, and ${\alpha}-2$ sub-units of acidic glutelin. Furthermore, the overall sensory evaluation score was observed to be positively correlated with albumin ($0.495^{**}$) and globulin ($0.567^{**}$), and negatively correlated with ${\alpha}-1$ glutelin ($-0.612^{**}$). Therefore, the results indicated that albumin, globulin, and ${\alpha}-1$ glutelin can affect the eating quality of japonica and tongil-type rice varieties, with the latter having lower eating quality than the former.

• Korean Journal of Animal Reproduction
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• v.20 no.4
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• pp.459-465
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• 1997

The present study was undertaken to determine the effect of NT time on the rate of fusion a and suhseguent development In vitro and determine the optimal strength and duration of DC pulse for electrofusion of IVF donor embryo nuclei and IVM recipient oocytes. The recipient oocytes were enucleated 25 ~ 2Sh after IVM and further cultured for 18 ~ 20h prior to fusion for oocyte aging. IVF embryos as donor nuclei were C co cultured with BOEC for 16- to 32-cell stage development. The transfer time of donor bIas tomeres was 1~3h post-enucleation in early NT group and 1 ~ 18h post-enucleation in late NT group, respectively and fusion was performed 43~4Sh post-IVM. The fusion rate did not differ between the early NT and late NT group, but the rate of cleavage and 8- to 16-cell stage embryos in the late NT group was more higher than that in the early NT group. The fusion, cleavage and M+B development was high from O.7SkV /cm DC than from 1.0kV /cm DC voltage, resulting in 17.6% M+B from 0.75kV /cm DC voltage. No difference in fusion rate was among pulse durations, but 50 and 70 usec pulse duration showed slight high cleavage and M + B d development. The results indicate that the best NT time of IVF donor blastomeres into the enucleated oocytes was 42~44 post-IVM and the most suitable condition for electrofusion was a single 0.7SkV /cm DC voltage for SO~70$\mu$sec.

• Proceedings of the KAIS Fall Conference
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• 2010.05b
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• pp.1077-1082
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• 2010

본 연구는 일반 여성과 유방암 환자를 대상으로 유방 자가검진의 이행율과 유방 자가검진에 영향을 미치는 요인을 파악하기 위하여 시도된 단면적 조사 연구이다. 건강신념 변수에 대해 일반 여성군과 유방암 환자군을 비교하면 일반 여성군이 유방암 환자군에 비해 유방암에 대한 심각성이 높은 것으로 나타났다. 유방암 환자군에서는 일반 여성군에 비해 유방암에 대한 민감성이 높았으며, 유방암 검진에 대한 유익성이 높고, 건강관심도가 높은 것으로 나타났다(p<0.01). 건강신념 변수에 대해 유방 자가검진 이행자와 불이행자를 비교해 보면 유방 자가검진 이행자는 불이행자보다 건강 관심도가 높고(p<0.01) 유방 자가검진에 대해 설명을 들은 경험이 있는 경우 자가검진 이행율이 높은 것으로 나타났다(p<0.05). 유방 자가검진 불이행자에서는 이행자보다 건강관심도가 낮고, 주위에서 유방 자가검진을 권하는 사람이 없는 경우가 많았다(p<0.01).유방 자가검진 이행자와 불이행자간 민감성, 심각성, 유익성, 장애성에 대해서는 유의한 차이가 없는 것으로 나타났다. 유방 자가검진 이행 여부에 관해서는 유방암 환자군이 일반 여성군에 비해 유방 자가검진을 3.5배 시행하지 않는 것으로 나타났고(p<0.01), 연령은 40대 미만보다 60대 이상여성이 1.6배 유방 자가검진을 시행하지 않는 것으로 나타났다. 교육수준은 대졸이상 보다 고졸이하가 2.4배 유방 자가검진을 시행하지 않고, 가구 소득은 200만원미만보다 400만원이상 고소득자가 2.7배 유방 자가검진을 시행하지 않는 것으로 나타났다. 자녀수는 자녀가 없는 여성이 자녀수가 3명이상인 여성에 비해 12.1배 유방 자가검진을 시행하지 않는 것으로 나타났다(p<0.05). 건강 신념과 유방 자가검진 실천의 관계를 본 결과 건강관심도와 행동계기가 유방 자가검진 수행에 유의한 영향을 미치는 것으로 나타났다. 건강관심도가 높을수록 유방 자가검진 실천도가 높고, 행동 계기 즉 주위에서 유방 자가검진을 권하는 사람이 있는 경우가 유방자가검진 수행율이 높은 것으로 나타났다(p<0.05). 본 연구 결과를 통해 유방암 환자를 포함한 일반여성을 대상으로 유방 자가검진의 중요성과 유방 자가검진 방법에 대해 정기적인 교육 프로그램, 포스터, 안내책자 등을 통한 적극적인 교육과 권유가 필요할 것으로 보여 진다. 유방 자가검진 프로그램 개발 시 유방암 환자와 일반 여성을 대상으로 한 차별화 된 교육 프로그램 개발이 필요하고 이를 통해 유방암 환자에게 유방암의 재발 위험성과 자가검진의 필요성, 올바른 유방암 환자의 자가검진법을 인지시킴으로서 유방 자가검진 실천율을 향상 시킬 수 있을 것이라고 생각된다.

• Journal of the Korean Society of Propulsion Engineers
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• v.10 no.3
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• pp.60-66
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• 2006

Notable are the burning rate characteristics of solid propellant burning at extremely high pressure(10000-20000 psia). The burning rate test using closed bomb shows the discontinuous increment around 4000 psia so that the exponent of burning rate(n) is almost double, from 0.4 to 0.8. The pressure-increasing rate of the test motor is about 300 times as high as that of the motor operating at the conventional pressure, less than 2000 psia, is, therefor the burning rate is augmented about 5-50 times. The performance prediction reflecting the pressure-change-rate effect are fairly comparable with the test data at various test conditions.

• Korean Journal of Food Science and Technology
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• v.48 no.3
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• pp.223-230
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• 2016

We investigated the fermentation characteristics of yakju made with nuruk extracts. Alcoholic fermentation was performed in an incubator set at $25^{\circ}C$ for 14 days. The study results are as follows: the alcohol content of yakju made with modified nuruk (18.67%) was higher than that made with other nuruk extracts (14.80-17.50%). The pH value of this modified nuruk (4.13) was higher than that of traditional nuruk (3.70-4.00). The yakju containing modified nuruk showed the highest acidity (3.64 mL/10 mL), amino acidity (4.72 mL/10 mL), soluble solid ($1.20^{\circ}Bx$), and color difference (39.78). The concentration of organic acids in traditional nuruks was 212.25-312.97 mg/100 mL, whereas that in modified nuruk was 295.92 mg/100 mL. Nitrogen compound concentrations in modified nuruk (2285.89 mg/L) were 2-14 times higher than that in traditional nuruk. Nitrogen compound concentration and amino acidity are the physiochemical characteristics with the highest impact on off-flavor and browning of yakju. In order to reduce these unfavorable characteristics, material with a lower protein content should be used.

• Journal of Bio-Environment Control
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• v.3 no.1
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• pp.1-9
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• 1994

Wind load or snow load, acting on agricultural structures is working more sensitive than any other load and therefore plays an important role in determination of design loads of agricultural structures. In this study, unit snow weight, greatest gust speed and depth of snow fall were analyzed and applied to determine the amount of frames. The unit snow weights were statistically classified and calculated in the basis of mean temperature and showed considerable differences between the unit snow weights at below and above -1$^{\circ}C$. Equations for estimating greatest gust speed with fastest wind speed were developed for inland and seaside districts. The calculated values from developed equations were little higher than those from the current equation in general. The difference between the depths of snow cover and snow fall, which shows the possibility of reduction of design loads under the adequate management. Design wind speed estimated by a modified equation suggested the amount of frames less than those by current one, and the depth of snow fall as a design snow depth suggested the amount of frames more than those of snow cover. Therefore, it is very important to select the adequate design values considering the characteristics of agricultural structures.