• Journal of fish pathology
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• v.20 no.1
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• pp.41-47
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• 2007

To investigate the cause of the mass mortality during rockbream, Oplegnathus fasciatus seed production, the water quality and bacterial counts of sea water in breeding tanks was measured 20days post the hatch. During breeding of rockbream fry, the environmental factors of water quality were detected as pH, ammonia COD, phosphate at the supply of the food organisms and the seawater. pH was decreased from the 8.21 of the 1 day per hatch (dph) to 7.56 of the on the 7 dph. Ammonia was conversely increased 0.49 ppm of the 1 dph to 0.85 ppm of 10 dph. As the adding of the chlorella and the rotifer tanks, COD was increased the 3.3 times and 1.2 times than those of pre-adding respectively. The phosphate and the ammonia were also increased 1.7 and 2.3 times, with adding the chlorella respectively, which exceeded the second grade for sea water evaluation level, 0.015 ppm and 0.1 ppm respectively. Water quality was not improved by PSB (Photosynthetic Bacteria) treatment, which increased the value of COD in 1.7 times, phosphate in 2.7 times and ammonia in 1.4 times. The number of the bacteria was also increased along the dph. According to the treatment of chlorella, the number of total bacteria increased in 1.4 times and those of Vibrio sp. 1.6 times. The lethal concentration of ammonia was investigated that over than 10 ppm could killed the fry of rockbream within 28 hrs, but 40% in 2 ppm.

• Journal of Bio-Environment Control
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• v.31 no.2
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• pp.79-89
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• 2022

With the wide use of greenhouses, the working hours have been increasing inside the greenhouse for workers. In the closed ventilated greenhouse, the internal environment has less affected to external weather during making a suitable temperature for crop growth. Greenhouse workers are exposed to organic dust including soil dust, pollen, pesticide residues, microorganisms during tillage process, soil grading, fertilizing, and harvesting operations. Therefore, the health status and working environment exposed to workers should be considered inside the greenhouse. It is necessary to secure basic data on particulate matter (PM) concentrations in order to set up dust reduction and health safety plans. To understand the PM concentration of working environment in greenhouse, the PM concnentrations were monitored in the cut-rose and Hallabong greenhouses in terms of PM size, working type, and working period. Compare to no-work (move) period, a significant increase in PM concentration was found during tillage operation in Hallabong greenhouse by 4.94 times on TSP (total suspended particle), 2.71 times on PM-10 (particle size of 10 ㎛ or larger), and 1.53 times on PM-2.5, respectively. During pruning operation in cut-rose greenhouse, TSP concentration was 7.4 times higher and PM-10 concentration was 3.2 times higher than during no-work period. As a result of analysis of PM contribution ratio by particle sizes, it was shown that PM-10 constitute the largest percentage. There was a significant difference in the PM concentration between work and no-work periods, and the concentration of PM during work was significant higher (p < 0.001). It was found that workers were generally exposed to a high level of dust concentration from 2.5 ㎛ to 35.15 ㎛ during tillage operation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.12
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• pp.1725-1731
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• 2016

This study was carried out to investigate the effects of steam-drying on antioxidant compounds and antioxidant activity of Alliun hookeri root (AHR). The yield of methanolic extracts, total phenolic content (TPC), browning intensity (280 nm and 420 nm), and organosulfur compound contents (alliin and cycloalliin) in raw and steam-dried AHRs were 10.71~37.40%, $15.53{\sim}36.36{\mu}g/mg$, 0.48~2.09, 0.01~0.25, $1.46{\sim}700.61{\mu}g/mg$, and $1,173.95{\sim}2,182.60{\mu}g/mg$, respectively. Yield, TPC, and browning intensity of AHRs increased by steam-drying, whereas organosulfur compound contents decreased. Of all methanolic extracts from raw and steam-dried AHRs, four-time steam-drying showed the lowest $EC_{50}$ values (0.43, 7.53, 0.34, and 0.48 mg/mL, respectively) for DPPH radical scavenging activity, ABTS radical scavenging activity, SOD-like activity, and reducing power, whereas four-time steam-drying resulted in the highest TPC ($36.36{\mu}g/mg$) and browning intensity (2.09 and 0.25 at 280 and 420 nm, respectively). The antioxidant activities of methanolic extracts from raw and steam-dried AHRs were closely correlated with their TPC, browning intensity, and organosulfur compound content, showing correlation determination coefficient ($R^2$) values higher than 0.82. As a result, four-time steam-drying of AHRs could be useful as potential antioxidant sources.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.123-130
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• 2005

High salt concentrations in the ginseng nursery soil environment of Korea is one of important reducing factors for the stable production of quality ginseng. These studies were accomplished for check the response on germination of ginseng seed, somatic embryogenesis of zygotic embryo, and biosynthesis of ginsenoside from ginseng hairy root against NaCl. Ratio of germination was at the $3\%\;and\;84.5\%$ on the basic media with 0.1M and free of NaCl repectedly, but $0\%$ at the upper of 0.2M NaCl. Somatic embryogenesis from zygotic embryo were the highest when immatured embryo was cultured on free of NaCl concentration, and which was intend to decrease at treatment of NaCl. However, in case of using the matured embryo, treatment of 0.05M NaCl resulted in better embryogenesis than NaCl free media. Red pigment was synthesized from ginseng hairy root cultured on the medium with various NaCl concentration(from 0.04 to 0.08M) and its pigment was analyzed as spectrum of anthocyane by spectrophoto- meter scanning. This cell line biosynthesized lots of crude saponin and total ginsenoside than other cell lines, also had 2 times of panaxadiol than panaxatriol.

• Journal of Korean Society of Forest Science
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• v.84 no.1
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• pp.41-47
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• 1995

A micropropagation system for black Locust(Robinia pseudoacacia) was established by using shoots and pin-punctured leaves of in vitro germinated seedlings. The greatest number of shoots (an average of 10.5 shoots) was obtained when shoot tips were cultured on MS medium supplemented with 1.0 mg/l BAP and 0.01 mg/l NAA. When pin-punctured leaf explants were cultured on the same medium, mean number of 13.5 shoots were produced. Shoot growth was accelerated by adding 50 mg/l of silver nitrate ($AgNO_3$), an anti-ethylene compound to the culture medium. Each shoot was excised from the mass and transferred onto half strength MS medium for rooting. Zygotic embryos at different developmental stages were cultured on LS medium supplemented with various growth regulators to induce somatic embryos. When cultured on LS medium with 1.0 mg/l 2,4-D. 14.3% of the zygotic embryos induced somatic embryos. Upon transfer onto the basal medium, somatic embryos sporadically converted into plantlets.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.275-280
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• 1999

In order to find optimum conditions for somatic embryogenesis from different individual (2-year-old) in Aralia elata were cultured on MS medium supplemented with 1.0 mg/L 2,4-D, 3% sucrose, and 0.3% gelrite. We also investigated the effect of MS medium salt concentration, BA and ABA on the embryo germination and plant regeneration. While noticeable difference was observed on somatic embryo induction among different individual tree, no apparent difference was seen in both germination and regeneration frequencies. Compared with nonembryogenic calli, embryogenic calli tended to look yellow and/or pale brown in color, slowly growing and soft in their texture. Regardless of BA or ABA treatment, half-strength MS salt medium proved to be better than full strength MS medium in both embryo germination and plant regeneration. Both hypocotyl and cotyledon developments were slightly promoted by adding 0.1 mg/L BA. However, its effect on germination and regeneration seemed inferior to control. ABA treatment on somatic embryos at their torpedo and early cotyledonary stages resulted in poor response in germination and regeneration. Although most regenerated plantlets varied greatly in cotyledon number and shape, they could be developed into normal plants after 4 weeks in culture. More than 95% plantlets were acclimatized in an artificial soil mixture, successfully transplanted to nursery bed and grew normally without any phenotypic abnormalty.

• The Korean Journal of Nuclear Medicine
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• v.37 no.4
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• pp.219-228
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• 2003

Purpose: The amount of salvaged myocardium is an important prognostic factor in patients with acute myocardial infarction (MI). We investigated if early Tl-201 SPECT imaging could be used to predict the salvaged myocardium and functional recovery in acute MI after primary PTCA. Materials and Methods: In 36 patients with first acute MI treated with primary PTCA, serial echocardiography and Tl-201 SPECT imaging ($5.8{\pm}2.1$ days after PTDA) were performed. Regional wall motion and perfusion were quantified with on 16-segment myocardial model with 5-point and 4-point scaling system, respectively. Results: Wall motion was improved in 78 of the 212 dyssynergic segments on 1 month follow-up echocardiography and 97 on 7 months follow-up echocardiography, which were proved to be salvaged myocardium. The areas under receiver operating characteristic curves of Tl-201 perfusion score for detecting salvaged myocardial segments were 0.79 for 1 month follow-up and 0.83 for 7 months follow-up. The sensitivity and specificity of Tl-201 redistribution images with optimum cutoff of 40% of peak thallium activity for detecting salvaged myocardium were 84.6% and 55.2% for 1 month follow-up, and 87.6% and 64.3% for 7 months follow-up, respectively. There was a linear relationship between the percentage of peak thallium activity on early redistribution imaging and the likelihood of segmental functional improvement 7 months after reperfusion. Conclusion: Tl-201 myocardial perfusion SPECT imaging performed early within 10 days after reperfusion can be used to predict the salvaged myocardium and functional recovery with high sensitivity during the 7 months following primary PTCA in patients with acute MI.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.555-564
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• 2010

Aphid and mite are the major insects that harm corn. These insects are difficult to control because they are very sensitive to environmental conditions. The purpose of this experiment was to information the loss in quantity of corn and to select inbred lines with resistance after exposing corn to aphid and mite. When exposed to aphid, the average loss in grain yield, ear length, ear diameter, 100 kernel weight, kernel length, kernel width, and kernel thickness were 18.4, 10.9, 1.7, 5.4, 2.3, 1.5 and -0.2%, respectively. When exposed to mite, the average loss in grain yield, ear length, ear diameter, 100 kernel weight, kernel length, kernel width, and kernel thickness were 49.7, 16.5, 20.7, 18.9, 9.5, and -3.4, respectively. Mite had more significant impact on the loss than aphid. Among these factors, grain yield and ear dimater were most significantly correlated on the loss to aphid ($r=0.83^{**}$), while kernel length and thickness were most significantly correlated on the loss to mite ($r=0.83^{**}$). However, ear length was not related to loss to the two insects. The inbred lines that were often used for $F_1$ hybrid which was lower than the average loss by aphid, were KS7, B68, 61B3, 70A1, and KS5, while the inbred lines that were often used for $F_1$ hybrid which was lower than the average decrease by mite, were KS7, B68, 61B3, 66B2-4, 70A1, 72B2, and KS5. These inbred lines could be used to breed inbred lines, hybrid, and open pollination variety that confer resistant to aphid and mite.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.2
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• pp.560-569
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• 2016

Purpose The purpose of this study was not only to explore the factors associated with the survival of OHCA(Out-of-hospital Cardiac Arrest), but to provide ideas for improving the operation of emergency medical system in Korea. Method 90,734 OHCAs(Out-of-hospital Cardiac Arrest) with a cardiac etiology, who had been transported by 119 EMS ambulances for seven years from 2006 to 2012 in Korea, were analyzed. The data had a multilevel structure in that patient's survival in the same region is interrelated, so two-level (patient-region) logistic regression analysis was applied to adjust this correlation. Results The adjusted OR in group who were given CPR(Cardiopulmonary Resuscitation) by a bystander were 1.40 for survival to discharge. In addition, the adjusted OR in the group with an implementation of AED (automated external defibrillator) before arriving in hospital was 2.98 for survival to discharge. we categorized some continuous variables (number of emergency physician, OHCAs volume fo hospital, area deprivation level) into five quintiles. The adjusted OR in the number of emergency physician compared with Q1(lowest) was 1.29(Q2), 2.89(Q3), 3.39(Q4), 4.07(Q5), respectively. the adjusted OR in OHCAs volume of each hospital compared with Q1(lowest) was 2.06(Q2), 3.06(Q3), 3.46(Q4), 4.36(Q5), respectively. Lastly, the adjusted OR in deprivation level compared with Q1(least deprived area) was 0.72(Q4), 0.64(Q5) so that the adjusted OR of survival to discharge tended to decrease in more deprived districts. Conclusion The survival to discharge was better significantly in group given CPR by a bystander and with the implementation of AED before arriving in hospital. The survival to discharge tended to be significantly better in hospitals with a larger number of emergency physicians and higher volume of OHCAs in less deprived districts.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.122-127
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• 1997

This study was conducted to establish mass propagation system from the tissue culture using immature embroys in Eleutherococus senticosus. Immature embroys from seeds were removed under the microscope and placed on modified SH and WPM medium containing several plant growth regulators. The calli were well formed on media containing 1mg/l of 2,4-D on modified SH medium and 1mg/l of 2,4-D and 3mg/l of TDZ on WPM medium. Shoot regeneration was better on modified SH or WPM medium with combination of high concentration of TDZ and low concentration of 2,4-D. Treatment of 2,4-D alone was better than treatment of TDZ alone in callus induction on modified SH medium but plant regeneration reversed. Treatment of 2.4-D and TDZ combination was better than treatment of 2,4-D alone in callus induction on WPM medium. The results of callus formation and shoot regeneration on WPM media differed to those of SH media. The rate of callus formation was nearly 83% when 2,4-D was added to SH medium on concentration of 1mg/l. The rate of callus formation was nearly 38% when combination treatment of 2,4-D 1mg/l and TDZ 3mg/l was added to WPM medium. Also, plant regeneration differed depending on the mature degree of immature embryo.