• Korean Journal of Ecology and Environment
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• 제35권3호통권99호
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• pp.198-212
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• 2002

A numerical variation and abnormalities were studied on egg bags and embryos of Korean salamander, Hynobius leechii from agricultural habitat. The teratogenic and toxic effects of fungicide benomyl were also investigated with early embryos from non-agricultural habitat. We collected 144 egg bags from agricultural region, and 3418 of early embryos were contained. The lengths of egg bags were varied from 10 to 23 cm and the most frequent length was 19 cm. The number of embryos was varied from 7 to 43, and the most frequent range was 22 to 26. Spontaneous abnormalities were occurred in 406 embryos among 116 egg bags, and 24 kinds of external abnormalities were found. Individuals showing severe external defect were histologically studied and they showed optic dyspalsia, thyroid carcinoma, somatic muscular dysplasia, partial biaxial structure, decrease of red blood cells in the heart, cephalic degeneration and intestinal dysplasia. 385 embryos from non-agricultural region were exposed to 200 nM${\sim}$ 1 ${\mu}$M of benomyl at blastula or gastrula for 12 days. All embryo were dead in the concentration of 1 ${\mu}$M (LD$_{100}$) and 75% of embryos were dead in 800nM of benomyl. Speciflc effect due to benomyl was acrania or cephalic dysplasia and this restult suggests that the benomyl inhibit stongly to the development of neural tissue. These abnormal developments may be caused by antimitotic action, inhibition of tubulin complex, destruction of microtubule, inhibitions of neurulation and closing of neural fold, and by the inhibition of the movement of neural crest cells.

• Korean Journal of Animal Reproduction
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• 제24권3호
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• pp.311-317
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• 2000

This study was carried out to compare the temperature and time of heat shock, and the effect of heat shock on development of embryos after in vitro maturation and fertilization in bovine oocytes. The results obtained were as follows. 1. The optimum temperature and time of heat shock were 41$^{\circ}C$ and 30sec on in vitro development of embryos from 4~8 cell to blastocyst. 2. The rates of cleavage on zygotes produced on in vitro were significantly increased by heat shock after IVM than before IVM(P<0.05). 3. When the oocytes were treated heat shock after IVM and 5 days cultured, developmental rates to blastocyst were increased than other experimental treatments.

• Korean Journal of Plant Resources
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• 제18권1호
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• pp.123-130
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• 2005

High salt concentrations in the ginseng nursery soil environment of Korea is one of important reducing factors for the stable production of quality ginseng. These studies were accomplished for check the response on germination of ginseng seed, somatic embryogenesis of zygotic embryo, and biosynthesis of ginsenoside from ginseng hairy root against NaCl. Ratio of germination was at the $3\%\;and\;84.5\%$ on the basic media with 0.1M and free of NaCl repectedly, but $0\%$ at the upper of 0.2M NaCl. Somatic embryogenesis from zygotic embryo were the highest when immatured embryo was cultured on free of NaCl concentration, and which was intend to decrease at treatment of NaCl. However, in case of using the matured embryo, treatment of 0.05M NaCl resulted in better embryogenesis than NaCl free media. Red pigment was synthesized from ginseng hairy root cultured on the medium with various NaCl concentration(from 0.04 to 0.08M) and its pigment was analyzed as spectrum of anthocyane by spectrophoto- meter scanning. This cell line biosynthesized lots of crude saponin and total ginsenoside than other cell lines, also had 2 times of panaxadiol than panaxatriol.

• Korean Journal of Plant Tissue Culture
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• 제21권3호
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• pp.167-171
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• 1994

This study was carried out to investigate the possibility of plant regeneration through somatic embryogenesis in suspension culture of Aralia elata S. Callus was induced from the explants of leaf and petiole cultured in the MS media containing 2,4-D and TDZ. More embryogenic calli were formed from petiole and with combination treatment of 24-D and TDZ. The quarter strength MS medium was effective for increasing number of somatic embryos. Mannitol supplemented to the quarter strength MS medium, reduced somatic embryo formation but inositol increased. Normal plantlets(86%) were regenerated from mature somatic embryos in MS basal medium and 50% of those survied when transplanted to the vermiculite in greenhouse.

• Korean Journal of Animal Reproduction
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• 제26권1호
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• pp.41-51
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• 2002

The present study was carried out to examine the effect of cysteamine in vitro maturation (IVM) of porcine oocytes and development of porcine IVM/IVF Embryos. The results were summarized as follows : 1. The rates of nuclear maturation, penetrated oocytes, pronuclear formation, polyspermic oocytes and mean numbers of the penetrated sperm were not different in NCSU23 maturation medium with 0, 25, 50 and 100 $\mu$M cysteamine (P〉0.05). 2. The rates of blastocyst formation at day 7 after in vitro fertilization in 0, 25, 50 and 100 $\mu$M cysteamine were 17.9$\pm$6.1, 17.4$\pm$6.3, 24.2$\pm$1.9 and 16.9$\pm$2.0%, respectively. And the total cells were 30.7$\pm$2.4, 34.9$\pm$2.8, 39.6$\pm$2.3 and 36.8$\pm$3.6, respectively. Fifty $\mu$M cystealnine group was significantly higher than those of any other treatment groups (P＜0.05). 3. The ratios of ICM/total cells in 20~40% category were 20.5, 41.6, 19.5 and 31.5%, respectively. Twenty five $\mu$M cysteamine group was higher than those of other groups. 4. The rates of blastocyst formation at day 7 in the NCSU-23 culture medium of porcine IVF-produced embryos with 0, 25, 50, and 100 $\mu$M cysteamine were 16.0$\pm$0.2, 13.6$\pm$1.7, 25.0$\pm$0.8 and 15.7$\pm$4.5%, respectively. And the total cells were 27.0$\pm$3.7, 36.1$\pm$4.8, 34.0$\pm$3.8 and 25.2$\pm$4.4, respectively. Fifty $\mu$M cysteamine group was significantly higher than those of any other treatment groups (P＜0.05). 5. The ratios of ICM/total cells in 20~40% category were 53.8, 30.0, 16.6 and 11.1%, respectively. The addition groups of cysteamine were lower than those of control group. In conclusion, these results suggested that the addition of 50 $\mu$M cysteamine in the IVM medium and 25~50 $\mu$M cysteamine in IVC medium were effective on the blastocyst formation and total cells of blastocysts.

• Korean Journal of Clinical Laboratory Science
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• 제48권4호
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• pp.304-311
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• 2016

The study was performed to examine the metabolic syndrome risk factors in accordance with the obesity types based on body mass index (BMI) and waist circumference cutoffs. The diagnosis of metabolic syndrome closely adhered to the NCEP-ATP III criteria, and obesity was defined using the WHO Asian-Pacific criteria. We used the data from 591 elderly women, all aged over 65 years. They were divided into four groups: The normal group (n=272), the obesity group (n=124), abdominal obesity group (n=19), and obesity-abdominal obesity group (n=176). The obesity-abdominal obesity group was the most prevalent group of low HDL-cholesterol (p=0.009), hypertriglyceridemia (p=0.025), abdominal obesity (p<0.001), and metabolic syndrome (p<0.001). Logistic regression analysis demonstrated that the obesity-abdominal obesity group had the highest odds ratio in predicting metabolic syndrome (OR: 10.638, 95% CI: 6.053~18.697). Therefore, the obesity-abdominal obesity group was the strongest predictive factor of metabolic syndrome risk in Korean elderly women.

• Korean journal of applied entomology
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• 제33권4호
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• pp.242-249
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• 1994

These studles were conducted to investigate the development of chem~cal resistance and cross.resista. nce m dlarnondback moth (Piuteiia xylostella L). The resistance development of diamondback moth greatly vaned under single selection of five insectlades. The tnflumuron and lambda cyhalothnn strains at 8th selected generation showed 374- and 29.l.fold resistant levels. respectively, as compared with the susceptible strain. However, the Baciilus thuringiensis-seleded at 8th selected generatLon exhibited 240-fold resistant level. and the prothiophos-selected at 8th generation revealed 14.3-fold resistant !eve1 while the cartap hydrochloride-selected at 8th generailon showed 9.1-fold resistant level Prothiophos- selected strain showed low cross-resistance level to cartap hydrochloride, while this strain exhibited no cross-resistance of 1.3 to 2.8-fold to other msectlcides. Cartap hydrochlonde- seleded strain showed 19.9-fold. a high cross-resistance to lambda cyhalothrin, but this strain showed 2.2-34 fold, no cross resistance to other insecticide. Lambda cyhalothnn-selected strain exhibited cross-resistance to cartap hydrochlolide and prothiophas. Triflumuron-seleded strain showed 1.3-4.9 fold. no cross-resistance to other ~nsectic~dTe he B. ihuring~ensis-selededs train showed no cross-resistance to other insecticides.

• Journal of the Korea Convergence Society
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• 제10권5호
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• pp.43-50
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• 2019

The purpose of this study was investigate the association between birth age related health factors and metabolic syndrome in women. The data of 6743 women from Korean National Health and Nutrition Examination Survey(2012-2014 KNHANES) were analyzed. Dependent variable was metabolic syndrom related variables were collected. Menopause status was associated with metabolic syndrome [odds ratio of with post-menopause 1.44(95% CI: 1.09-1.90)]. First birth age was associated with metabolic syndrome [odds ratio of women with 26-30 year : 0.81 (95% CI: 0.68-0.98); odds ratio of women 31 year over: 0.62(95% CI: 0.45-0.87)] after adjusted for age, region, household income, smoking, occupation. In conclusion, the metabolic syndrome is closely related to menopause and first birth age, and will be used as a basic data in developing future educational programs for women.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 한국생물환경조절학회 2001년도 봄 학술발표논문집
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• pp.65-66
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• 2001

제주도의 감자재배면적은 전국의 30.6%를 차지하고 있으며 본도 제2의 소득작물로 자리잡고 있는데 최근 국내외적으로 친환경농산물에 대한 관심이 고조됨에 따라 안전농산물에 대한 수요가 늘면서 유기농업자제를 이용한 고품질 안전 농산물을 생산하려는 시도가 늘어가고 있다. 이런 관점에서, 키토산을 \circled1토양처리 (1kg/a), \circled2키토산침지 (0.2%/30분), \circled3토양처 리＋생육기처리 100배 (2회), \circled4토양처리＋생육기처리 200배 (2회), \circled5생육기처리 100배 (2회), \circled6생육기처리＋200배 (2회), \circled7무처리로 구분하여 시험하였다. 발아율은 대조구에 비해서 종서침지, 토양관주＋생육기 200배처리, 생육기 200배처리한 것이 다소 높은 편이었고, 경장 경수 및 복지수는 시일이 경과할수록 많은 편이고 그 중에서 종서침지가 가장 양호한 편이었다. 그리고 10a당 총수량, 상서수량 및 설서수량(30g이하인 괴경)은 역시 종서침지, 토양관주＋생육기 200배 (2회)처리 및 토양관주＋생육기 100배처리 순서로 상서수량이 많은 편이었다. 처리별 상서수량은 종서침지한 것이 71%, 29%로 가장 많았고 토양관주＋생육기 100배처리한 것이 대조구보다 많은 경향이었다(Table 1).

• Journal of the Mineralogical Society of Korea
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• 제26권4호
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• pp.273-283
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• 2013

The artificial rain (pH 4.0, pH 5.6 and pH 6.85) and weathering simulation test are applied in dolomitic marble for the prediction of deterioration of the stone monuments constructed with carbonate rock by acid rain. pH of the applied rain all increase to about neutral pH after reaction of marble. The contents of $Ca^{2+}$ and $Mg^{2+}$ have increased more than twofold in two acid rain and deionized neutral rain after reaction of marble. The weight of marble is expected to decrease $0.00037kg/m^2$ each test cycle by pH 4.0 rain. This weight reduction rate of marble is 1.4 and 3.1 times more in pH 5.6 and pH 6.85 rain respectively, and 3.7 times more in only artificial weathering test. The compressive strength of marble is expected to decrease 0.2468, 0.1791 and $0.1280kg/m^2$ per test cycle with pH 4.0, pH 5.6 and pH 6.85 rain, respectively. These results mean that more acidic rain more enfeeble the strength of marble. Dolomite and small amount of calcite are precipitated in the rains after reaction of marble.