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Plant Regeneration via Somatic Embryogenesis and Organogenesis from Peucedanum japonicum $T_HUNB$ (갯기름나물의 체세포배 분화와 기관 분화를 통한 재분화)

  • Kim, Ok-Tae;Kim, Kwang-Soo;An, Jun-Chul;Hwang, Baik
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.1
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    • pp.21-24
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    • 2001
  • Peucedanum japonicum $T_{HUNB}$ used as a edible and medicinal plants was investigated for in uitro regeneration. Callus formation occurred on leaf and stem explant cultures and showed spontaneous embryogenic and organogenic capability on MS basal medium supplemented with 0.1~5 mg/L NAA and 0~10 mg/L BA in dark. The regeneration was highest on the condition supplemented with 2.5 mg/L NAA and 10 mg/L BA. Development of the somatic embryo progressed through the globular, heart-shaped, torpedo-shaped and cotyledonary stage, typical of zygotic embryos. When the first somatic embryos was cultured on the medium supplemented with 0.2 mg/L NAA, secondary somatic embryo were induced with higher frequency on the hypocotyl then on the cotyledon and root.t.

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Effect of Sarcodon Aspratus on the Physical and Sensory Properties of Cooked Chicken (능이버섯 첨가가 닭고기의 물리화학적 및 관능적 특성에 미치는 영향)

  • 이종호;박영희
    • Journal of Applied Tourism Food and Beverage Management and Research
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    • v.13 no.1
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    • pp.43-54
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    • 2002
  • An instrumental analysis of cooked chicken was carried out along with sensory evaluation to find out the effect of Sarcodon aspratus on the physicochemical and senory characteristics in comparision with kiwi fruit and pear. The moisture content of cooked chicken was increased in proportion to the increment of Sarcodon aspratus, kiwi fruit and pear. In the texture, shear force of cooked chicken was decreased with the increment of Sarcodon aspratus, kiwi fruit and pear. In terms of color, L-value was decreased by addition of Sarcodon aspratus, kiwi fruit and pear. but a-value was increased by addition of Sarcodon aspratus, kiwi fruit and pear. b-value was increased by addition of Sarcodon aspratus and pear. whereas b-value was decreased by addition of kiwi fruit. There were significant differences(p<0.05) in the sensory characteristics of the samples in which control was most preferred in taste and flavor. As the content of Sarcodon aspratus, kiwi fruit and pear was increased, the score of juiciness and tenderness was increased. In the overall acceptance, score of 0.05% Sarcodon aspratus and 10% pear was not different from that of control. Therefore, it can be concluded that 0.05-0.1% addition of Sarcodon aspratus might be desirable for the improvement of texture and juiciness of cooked chicken.

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Characterization of Stretchable Polypyrrole Films Prepared by Chemical and Electrochemical Method (화학적 및 전기화학적 방법으로 합성한 연신성 폴리피롤 필름의 특성)

  • 장관식;문봉진;오응주;홍장후
    • Polymer(Korea)
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    • v.27 no.4
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    • pp.323-329
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    • 2003
  • Stretchable Polypyrrole films using functionalized doping agent, di(2-ethylhexyl) sulfosuccinate sodium salt (NaDEHS), were synthesized by chemical and electrochemical method. Chemically and electrochemically Prepared Polypyrrole films were stretch-oriented (L/L$\_$0/= 1.0 ∼ 2.5) by zone drawing method and the electrical conductivities were measured. As the draw ratio was increased, the electrical conductivities were increased. This result was confirmed by the increase in crystallinity through the increase in draw ratio. The temperature dependence of electrical conductivity showed that 3D-variable range hopping model (L/L$\_$0/ = 1.0∼2.0) and ID-VRH model (L/L$\_$0/ = 2.5) gave the best fit to the data for stretched Ppy-DEHS films.

The Relations between metabolic syndrome, physical activity, and dietary patterns in Korean adults (한국 성인의 대사증후군과 신체활동, 식이 형태의 관계)

  • Bang, So-Youn
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.2
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    • pp.662-672
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    • 2019
  • This study involved a secondary analysis of 2016 Korean National Health and Nutrition Examination Survey data to identify the relations between metabolic syndrome, physical activity, and dietary patterns in Korean adults. The ${\chi}^2$-test, t-test, and logistic regression analysis of complex samples were conducted on the data of 3,352 subjects aged 19 to 64 years with metabolic syndrome. The prevalences of metabolic syndrome among men and women were 29.7% and 16.7%, respectively. In men, the high physical activity group showed a 0.8-fold increase in the likelihood of metabolic syndrome (OR=0.8, p=.038), and higher fat intake increased the risk of metabolic syndrome 0.98-fold (OR=0.98, p=.007). In women, the high physical activity group showed a 0.7-fold increase in the likelihood of metabolic syndrome (OR=0.71, p=.034), higher carbohydrate intake increased the risk of metabolic syndrome by 1.03-fold (OR=1.03, p<.001), and that higher protein and fat intakes reduced the risk by 0.96-fold (OR=0.96, p=.009) and 0.95-fold (OR=0.95, p<.001), respectively. The study shows that to meet the challenge presented by the increasing prevalence of metabolic syndrome, efforts are needed to screen for adults at risk of the metabolic syndrome by regular physical examination and to develop and provide intervention programs that involve regular high intensity physical activity and controlled carbohydrate, protein, and fat intakes.

Plant Regeneration via Secondary Somatic Embryogenesis and Acclimatization in Panax ginseng (장뇌삼의 2차 체세포배 발생을 통한 식물체 유도 및 순화)

  • Lee, Su-Gwang;Kim, Ji-Hee;Kang, Ho-Duck
    • Journal of Korean Society of Forest Science
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    • v.97 no.1
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    • pp.127-133
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    • 2008
  • This study was conducted to establish the optimal condition for plant regeneration and acclimatization from somatic embryos of Panax ginseng. Cotyledon segments of Panax ginseng produced primary and secondary somatic embryos when cultured on MS and WPM media supplemented with 7% sucrose. To induce plantlet conversion, cotyledonary somatic embryos were cultured on WPM solid medium with $GA_3$ at various concentrations (1~30 mg/L) for 4 weeks. Plantlets were transferred to 1/2 WPM solid medium with $GA_3$ at various concentrations (0~5 mg/L) and 0.5% activated charcoal for shoot and root elongations. Elongated plantlets further developed into well-developed leaf and root system on 1/3 SH medium with 0.5% activated charcoal under ventilation condition for 5 months. The highest survival rate to soil was 75% when plantlets were regenerated on 1/3 SH medium without sucrose under ventilation condition.

Effect of Embryo Morphology on Plant Development in Secondary Somatic Embryogenesis of Alfalfa (알팔파 캘러스로부터 형성된 이차체세포배의 형태가 유식물 발달에 미치는 영향)

  • Won, S.H.;Lee, B.H.;Jo, J.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.4
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    • pp.297-302
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    • 1999
  • Cotyledonary abnormalities were observed in secondary somatic embryos which were developed from calli cultured on MS medium with various concentrations of 2,4-D. In MS medium containing hormone-free or $0.1mg/{\ell}$ 2,4-D, the frequency of normal embryo formation with two cotyledons were above 57%. According to concentration of 2,4-D increment the frequency of normal embryos were decreased. In MS medium containing $4mg/{\ell}$ 2,4-D, the frequency of normal embryo formation was just 10%. The rate of germination was as follows; 37.7% of somatic embryos had one cotyledon, 85% two cotyledons, 38% three cotyledons, 35% four cotyledons, 25% five cotyledons and 29% trumpet-like cotyledons. About 80% of the embryos with two cotyledons were converted into normal plants, but one, three or four cotyledons were only 6.8~10%. The five or trumpet-like embryos were not developed into normal plants.

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흑마늘의 이화학적 성분 및 항산화활성

  • Seong, Nak-Ju
    • Food preservation and processing industry
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    • v.7 no.1
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    • pp.45-53
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    • 2008
  • 흑마늘의 이화학적 성분의 변화 및 항산화 활성에 대한 연구를 분석한 결과, 흑마늘의 색은 제조공정을 거치면서 L, a 및 b값이 모두 감소하였는데 내부색의 경우 가공 초기에 크게 감소하였다. 수분은 가공 중 계속해서 감소하였고, pH도 점차 산성화되어 가공이 끝난 시료에서 pH $4.22{\pm}0.02$였다. 총 페놀 화합물과 플라보노이드는 1단계시료에 비해 최종단계에서 각각 약 1.9배, 2.6배 증가하였다. Total pyruvate와 thiosulfinate도 비슷한 패턴으로 각각 약 1.6배, 5.8배 증가하였다. 유리당 중 가장 함량이 높은 fructose는 1단계에서는 $1,403.03{\pm}6.24\;mg/100\;g$ 였던 것이 4단계에서는 $2,454.45{\pm}4.20\;mg/100\;g$으로 증가하였으나, 반면에 이당류인 sucrose와 maltose는 흑마늘의 숙성과 더불어 서서히 감소하는 경향이다. 흑마늘의 구성아미노산은 glutamic acid의 함량이 가장 많았고, 다음으로 proline과 aspartic acid였으며, 유리아미노산은 taurine, homocysteine 및 $\delta$-hydroxylysine은 1단계 혹은 2단계 시료에서는 검출되지 않았으나, 2단계 혹은 3단계 이후시료에서 미량 검출되었다. 흑마늘 가공중 물추출물과 에탄올 추출물의 DPPH에 대한 전자공여능은 숙성이 진행될수록, 추출물의 첨가 농도를 높일수록 점차 증가하여 4단계 시료의 $1,000\;{\mu}g/mL$ 농도에서 에탄올 추출물은 $45.63{\pm}0.43%$, 물추출물은 $67.40{\pm}0.21%$로 시험된 시료 중 가장 높은 활성을 보였다. 환원력은 100 및 $500\;{\mu}g/mL$ 농도에서 1, 2단계 시료를 제외한 모든 시료에서 물추출물이 에탄올 추출물 보다 높은 활성을 보였다.

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Effect of MS Medium Strength and Nitrogen Concentration on Bulblet Formation and Growth of Lilium In Vitro (MS 배지 내 무기물 농도 및 질소 농도가 나리의 자구 형성과 비대에 미치는 영향)

  • 염미란;박현춘;양찬석;백기엽
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.341-346
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    • 2001
  • To investigate the effects of MS medium strength and nitrogen concentration on bulblet formation from bulblet scale segment culture and bulblet growth from bulblet of Lilium oriental hybrid 'Casa Blanca', asiatic hybrid 'Mona', and longiflorum hybrid 'Hinomoto', 0.5∼2.0 strength of MS salts and 30∼120 mM nitrogen concentrations of MS medium were examined in vitro. The number of bulblets from bulb scale segment was favored in the strength of 0.5∼1.0 strength of MS salts or 30 mM total nitrogen concentration of MS medium in three cultivars. But the growth of bulblets formed in vitro was promoted in the 2 strength of MS medium or hight concentration of total nitrogen of MS medium up to 120 mM in three experimented cultivars.

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Somatic Embryogenesis and Plant Regeneration in Embryogenic Cell Suspension Cultures of Hovenia dulcis Thunb (헛개나무의 현탁배양세포로부터 체세포배발생과 식물체 재생)

  • Li, Cheng-Hao;Zhao, Bo;Kim, Na-Young;Kim, Myong-Jo;Cho, Dong-Ha;Lee, Dong-Wook;Lee, Jae-Geun;Lim, Jung-Dae;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.14 no.4
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    • pp.255-260
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    • 2006
  • Culture conditions for high frequency plant regeneration via somatic embryogenesis from embryogenic cell suspension cultures of Hovenia dulcis are described. Germinated somatic embryos were selected for induction of secondary embryogenesis. Friable embryogenic cells were induced directly from somatic embryos when transfer to 1/3 MS solid or liquid medium lacking plant growth regulators. The temperature strongly effected on induction of secondary embryognesis than other conditions in culture. All somatic embryos produced friable embryogenic cell clumps within 10 days when germinated somatic embryos cultured in 1/3 MS medium at $30^{\circ}C$ in suspension culture. No somatic embryos formed from embryogenic cell suspension cultures at $18^{\circ}C$. Numerous somatic embryos were induced and subsequently developed uniformly into germination stage from suspended cell clumps after 4 weeks of culture on $18^{\circ}C$. Plantlets conversion were observed on $18^{\circ}C$ when germinated somatic embryos were transferred to 1/3 MS solid medium without plant growth regulators or supplemented with 0.1-0.5 mg/l benzyladenine.

Physicochemical Compositions of Raw and Dried Wolha Persimmons (월하시 생감 및 곶감의 이화학적 성분 분석)

  • Im, Ji-Soon;Lee, Myung-Hee
    • Food Science and Preservation
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    • v.14 no.6
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    • pp.611-616
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    • 2007
  • This study analyzed the major chemical components of raw and dried persimmons (Diospyros kaki Thunb.). Raw and dried persimmons contained (respectively) 85.52% and 47.36% moisture, 0.10% and 0.13% crude fat, 0.30% and 1.89% crude protein, and 0.56% and 2.0% crude fiber. The main free sugar components in both raw and dried persimmons were glucose and fructose. Seventeen amino acids were identified in the dried persimmons, amongst which the dominant ones were aspartic acid, glutamic acid, histidine and arginine. The total amino acid content of raw and dried persimmons was 3,130.76 ppm and 12,849.33 ppm, respectively. The major fatty acids in total lipids were palmitic acid, palmitoleic acid and linolenic acid in both raw and dried persimmons. The raw persimmons had 23.22% palmitoleic acid and 32.70% linolenic acid, suggesting that they have a high ratio of unsaturated fatty acids. The mineral content of both raw and dried persimmons was Na < Fe < Ca < P < K.