• Biotechnology and Bioprocess Engineering:BBE
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• 제11권4호
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• pp.368-371
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• 2006

The standard procedure outlined by the United States Environmental Protection Agency (US EPA) in Method 1623 for analyzing Giardia lamblia cysts and Cryptosporidium parvum oocysts in water samples consists of filtration, elution, centrifugal concentration, immunomagnetic separation (IMS), and immunofluorescence assay (IFA) followed by microscopic examination. In this study, the extent of (oo)cyst loss in each step of this procedure was evaluated by comparing recovery yields in segmented analyses: (i) IMS + IFA, (ii) concentration + IMS + IFA, and (iii) filtration/elution + concentration + IMS + IFA. The complete (oo)cyst recovery by the full procedure was $52{\sim}57%$. The (oo) cyst loss in the IMS step was only $0{\sim}6%$, implying that IMS is a fairly reliable method for (oo)cyst purification. Centrifugal concentration of the eluted sample and pellet collection before IMS resulted in a loss of $8{\sim}14%$ of the (oo)cysts. The largest (oo)cyst loss occurred in the elution step, with $68{\sim}71%$ of the total loss. The permeated loss of (oo)cysts was negligible during filtration of the water sample with a $1.0-{\mu}m$ pore polyethersulfone (PES) capsule. These results demonstrated that the largest fraction of (oo)cyst loss in this procedure occurred due to poor elution from the filter matrix. Improvements in the elution methodology are therefore required to enhance the overall recovery yield and the reliability of the detection of these parasitic protozoa.

• 대한환경공학회지
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• 제28권8호
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• pp.843-851
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• 2006

년도별 검출결과 2001년도에 각 지점별로 많은 검출을 보였지만 2004년도로 갈수록 Cryptosporidium과 Giardia 모두 10(oo)cysts/20 L 이하의 낮은 검출을 보였으며, 계절별로는 여름철과 늦여름($6{\sim}9$월)이 가장 많이 검출되었으며 지점별로는 고령교가 가장 높게 검출되었다. Cryptosporidium과 수질지표분석항목은 낮은 상관성을 보였으나 탁도와의 상관성에서는 Cryptosporidium의 경우 $R^2$=0.4914, Giardia는 $R^2$=0.6176으로 높게 나타났으며 Cryptosporidium보다 Giardia가 더 높은 상관성을 보였다. 그리고 탁도가 20 NTU 이상 일 때 Cryptosporidium과 Giardia의 검출이 대체로 20(oo)cystsd/20L 이상 높게 나타남을 알 수 있었다. Cryptosporidium과 Giardia의 상관성은 $R^2$=0.8432로 높게 나타났다. 각 지점별로 Cryptosporidium과 Giardia는 동시에 검출되고 있었으며, Giardia의 농도가 대체로 높게 나타났다. 축산폐수나 하수의 영향이 큰 것으로 나타났다.

• 미생물학회지
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• 제39권1호
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• pp.27-35
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• 2003

환경수 중 크립토스포리디움 및 지아디아 분석방법은 현재 어떤 방법도 만족할만한 민감도, 특이도, 재현성을 인정 받지 못하고 있어, 다양한 형태의 정도관리 및 수행도 평가가 요구되고 있다. 본 연구는 1623방법(USEPA :Method 1623)을 사용해 한강 지표수에 대한 크립토스포리디움 및 지아디아 접종시험과 현장시료 동시분석시험에 기초하여, 환경수 중 이들의 존재여부와 농도를 측정함에 있어 분석결과의 정확도 및 신뢰도, 그리고 회수율에 미치는 영향요인을 검토하였다. 1623방법에 의한 결과, 평균적으로 한강 지표수시료에 존재하는 크립토스포 리디움의 46%와 지아디아의 60%를 검출할 수 있는 것으로 나타났으나, 각각의 회수율 범위가 13-73% 및 28-84%로 변이가 매우 커서 실제 환경 중 두 원생동물의 농도를 결정하기 위해서는 반복측정 및 통계학적 인 접 근이 수반되어 야 할 것으로 나타났다. 또한 IMS (immunomagnetic separation)중 산첨가에 의한 해체·분리과정을 1 회 더 반복함으로써 지표수시료에서의 총회수율의 약 10%를 향상시킬 수 있었으며, flow cytometry로 계수·준비된 (오)시스트((oo)cysts)를 정도관리에 사용할 경우 보다 신뢰할만한 결과를 얻을 수 있었다. 형광항체 및 DAPI염색은 현장시료특성 이나 전 처리과정에 따라 동일하지 않은 형광특징을 보인 바, 접종 된 현장시료 정제액에 대한 염색결과도 참조되어야 하며, 환경시료에는 다양한 비특이물질이 함유되어 있어 DAPI (4',6-diamidino-2-phenylindole) 염색 및 DIC(Differential Interference Contrast)에 의한 내부구조 관찰이 최종 동정기준이 되어야할 것으로 나타났다.

• 한국물환경학회지
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• 제22권2호
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• pp.271-276
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• 2006

The outbreak cases of Cryptosporidium or Giardia from drinking water in abroad have drawn attentions on the public health. It is well known that Cryptosporidium is the most resistant organism against chlorine disinfection. To guesstimate the levels of Cryptosporidium and Giardia in Korean surface water, 1~2 samples from 22 drinking water sources in four Korean major rivers of Han, Keum, Nakdong, and Youngsan were monitored. In addition, two sites in Kyunganchon, a contaminated river were monitored for comparison. In source waters, detection rates of Cryptosporidium and Giardia were 15% (6/39) and 21% (5/24) with the range of 1~3 oocysts/10 L and 1~6 cysts/10 L, respectively. In Kyunganchon, they were 60% (6/10) and 70% (7/10) in the range of 1~9 oocysts/10 L and 10~72 cysts/10 L, respectively. When one of the source waters in Han river was monitored monthly, Cryptosporidium were found mostly in cold season. Matrix of the samples gave influence on the recoveries of the spiked protozoa. The recoveries of both Cryptosporidium and Giardia increased in the samples of Kyunganchon, known as contaminated area. However, protozoan recovery did not show significant relation with turbidity, the index of matrix contamination, which implies that there are additional unveiled features of matrix affecting the recoveries of the protozoa. The protozoan distribution in Kyunganchon showed significant relations with Cl. perfringens, anaerobic and spore forming indicator bacteria of fecal contamination by regression analysis, but not with turbidity, the general indicator of water quality.

• 한국농공학회논문집
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• 제48권5호
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• pp.83-93
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• 2006

In unsaturated porous media, the air-water interface (AWI) plays an important role in removing of biocolloids such as bacteria, viruses, and protozoan (oo)cysts. In this study, four models related to calculation of specific AWI area are analyzed to determine the appropriate model, and the selected models are verified using the previously reported experimental data. The results indicate that the modified model from Niemet et al. (2002) is the most appropriate tool for calculating the specific AWI area using the van Genuchten (1980) parameters obtained from the water retention curve. Hence, it is expected that this model could be used to quantitatively determine the attachment of biocolloids to AWI in the transport modeling of biocolloids in unsaturated porous media.