• Journal of Microbiology and Biotechnology
• /
• v.20 no.3
• /
• pp.574-578
• /
• 2010

Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119 possesses stable enzymatic activity at up to $85^{\circ}C$. However, this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We characterized the regioselectivity of lauric acid by CYP119 using the auxiliary redox partner proteins putidaredoxin (Pd) and putidaredoxin reductase (PdR). Purified CYP119 protein showed a tight binding affinity to lauric acid ($K_d=1.1{\pm}0.1{\mu}M$) and dominantly hydroxylated (${\omega}-1$) position of lauric acid. We determined the steady-state kinetic parameters; $k_{cat}$ was 10.8 $min^{-1}$ and $K_m$, was 12 ${\mu}M$. The increased ratio to $\omega$-hydroxylated production of lauric acid catalyzed by CYP119 was observed with increase in the reaction temperature. These studies suggested that the regioselectivity of CYP119 provide the critical clue for the physiological enzyme function in this thermophilic archaebacteria. In addition, regioselectivity control of CYP119 without altering its thermostability can lead to the development of novel CYP119-based catalysts through protein engineering.

• Clinical and Experimental Pediatrics
• /
• v.59 no.10
• /
• pp.408-413
• /
• 2016

Purpose: This study investigated predictors of unresponsiveness to second-line intravenous immunoglobulin (IVIG) treatment for Kawasaki disease (KD). Methods: This was a single-center analysis of the medical records of 588 patients with KD who had been admitted to Asan Medical Center between 2006 and 2014. Related clinical and laboratory data were analyzed by univariate and multivariate logistic regression analyses. Results: Eighty (13.6%) of the 588 patients with KD were unresponsive to the initial IVIG treatment and received a second dose. For these 80 patients, univariate analysis of the laboratory results obtained before administering the second-line IVIG treatment showed that white blood cell count, neutrophil percent, hemoglobin level, platelet count, serum protein level, albumin level, potassium level, and C-reactive protein level were significant predictors. The addition of methyl prednisolone to the second-line regimen was not associated with treatment response (odds ratio [OR], 0.871; 95% confidence interval [CI], 0.216-3.512; P=0.846). Multivariate analysis revealed serum protein level to be the only predictor of unresponsiveness to the second-line treatment (OR, 0.160; 95% CI, 0.028-0.911; P=0.039). Receiver operating characteristic curve analysis to determine predictors of unresponsiveness to the second dose of IVIG showed a sensitivity of 100% and specificity of 72% at a serum protein cutoff level of <7.15 g/dL. Conclusion: The serum protein level of the patient prior to the second dose of IVIG is a significant predictor of unresponsiveness. The addition of methyl prednisolone to the second-line regimen produces no treatment benefit.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.2
• /
• pp.226-231
• /
• 2006

Several white-rot fungi are able to produce extracellular lignin-degrading enzymes such as manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase. In order to enhance the production of laccase and MnP using Trametes versicolor KCTC 16781 in suspension culture, the effects of major medium ingredients, such as carbon and nitrogen sources, on the production of the enzymes were investigated. The decolorization mechanism in terms of biodegradation and biosorption was also investigated. Among the carbon sources used, glucose showed the highest potential for the production of laccase and MnP. Ammonium tartrate was a good nitrogen source for the enzyme production. No significant difference in the laccase production was observed, when glucose concentration was varied between 5 g/l and 30 g/l. As the concentration of nitrogen source increased, a lower MnP activity was observed. The optimal C/N ratio was 25 for the production of laccase and MnP. When the concentrations of glucose and ammonium tartrate were simultaneously increased, the laccase and MnP activities increased dramatically. The maximum laccase and MnP activities were 33.7 U/ml at 72 h and 475 U/ml at 96 h, respectively, in the optimal condition. In this condition, over 90% decolorization efficiency was observed.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.31 no.10C
• /
• pp.1000-1010
• /
• 2006

This paper proposes a new architecture for DCT-based motion estimation and compensation. Previous methods do riot take sufficient advantage of the sparseness of 2-D DCT coefficients to reduce execution time. We first derive a recursion equation to perform DCT domain motion estimation more efficiently; we then use it to develop a wavefront array processor (WAP) consisting of processing elements. In addition, we show that the recursion equation enables motion predicted images with different frequency bands, for example, from the images with low frequency components to the images with low and high frequency components. The wavefront way Processor can reconfigure to different motion estimation algorithms, such as logarithmic search and three step search, without architectural modifications. These properties can be effectively used to reduce the energy required for video encoding and decoding. The proposed WAP architecture achieves a significant reduction in computational complexity and processing time. It is also shown that the motion estimation algorithm in the transform domain using SAD (Sum of Absolute Differences) matching criterion maximizes PSNR and the compression ratio for the practical video coding applications when compared to tile motion estimation algorithm in the spatial domain using either SAD or SSD.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.31 no.11
• /
• pp.895-903
• /
• 2007

CMBT(Curved Moving Boundary Treatment) is a newly developed scheme for the treatment of a no slip condition on the curved solid wall of moving obstacle in a flow field. In our research CMBT was used to perform LBM simulation of a flow over a moving circular cylinder to determine the flow feature and aerodynamics characteristic of the cylinder. To ascertain the applicability of CMBT on the complex shape of the obstacle, it was first simulated for the case of the flow over a fixed circular cylinder in a channel and the results were compared against the solution of Navier-Stokes equation with deforming mesh technique. The simulations were performed in a moderate range of reynolds number at each moving cylinder to identify the flow feature and aerodynamic characteristics of circular cylinder in a channel. The drag coefficients of the cylinder were calculated from the simulation results. We have numerically confirmed that the critical reynolds number for vortex shedding is ar Re=250 and the result is the same as the case of fixed cylinder. As the cylinder approaching to one wall, the 2nd vortex is developed by interacting with the wall boundary-layer vorticity. As the velocity ratio increase the third vortex are generated by interacting with the 2nd vortexes developed on the upper and lower wall boundary layer. The resultant $C_d$ decrease as reynolds number increasing and the Cd approached to a value when Re>1000.

• Applied Biological Chemistry
• /
• v.43 no.1
• /
• pp.1-6
• /
• 2000

The quality changes of chunggugjang produced by Bacillus sp. CS-17 was investigated with fermentation time. The pH was gradually alkalized. L-value became low while b-value became high. Both strength and hardness extraordinarily decreased during fermentation. Total content of free amino acid was $8274.7{\sim}9301.4\;mg%$ and phenylalanine, lysine, leucine and tyrosine were the most abundant components among the amino acids. The ratio of essential amino acid was $66.6{\sim}71.8%$. As a result of sensory test, it was found that the chunggugjang fermented by Bacillus sp. CS-17 was suitable enough to be produced for commercial purpose.

• Korean Journal of Poultry Science
• /
• v.12 no.2
• /
• pp.75-82
• /
• 1985

This study was carried out to estimate combining abilities and heterosis in White Cornish and White Plymouth Rock. The data were obtained over an 8weeks period from 3rd Oct., 1984 to 28th Nov. 1984 at the Livestock Experiment Station. A total of 810 birds, composed of 369 pure strains, 270 two-way crosses, and 180 fourway crosses were average amount of heterosis expressed as a ratio was 1.63% for livability, 5.47% for body weight at 8 weeks, 5.57% for weight gain from day old to 8 weeks and -5.98% for feed requirement. Specific combining ability were statistically significant for all of the traits examined in this study. Although the general combining ability was not significant for any of the traits, strain A appears to be superior to other strains.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.12 no.4
• /
• pp.2078-2083
• /
• 1970

The purpose of this study is to give the data neccesary for improving the planning of drainage structures, specially inverted siphons, in irrigation channels. With the samples of 15 drainage inlets, one drainage flume, 16 drainage inverted siphons and 6 drainage culverts in the 3 lines of irrigation channel under Chong-Won Irrigation Association, author abtained the following results. 1. It is presumed that design drainage discharge should be determined with some additional reserves, on the basis of the maximum rainfall intensity in local area and the size of drainage area on the topographical map, avoiding the way of eye measure. 2. Location of drainage inlet should be kept away from the place where topography can make lots of wash load, but when unavoidably allowing the inflow into irrigation channel, wash load outlet with even the purpose of drainage, or drainage flume in stead of drainage inlet should be taken account of. 3. It is presumed that drainage flume may be the structure which can perform its function from a structural point of view as far as topography permits. 4. Drainage inverted siphon should be avoided at any place as much as possible; a) In case that location of the siphon would be permitted only at paddy field, drainage area hauing the amount of discharge which requires more than 90cm in diameter could only be allowed. b) In this case, crest elevation of the tank of both inlet and outlet, at least, should not be lower than the surface level of paddy field. c) As far as topography and stratum permit, ratio of depth of outlet tank to head drop should be decreased as much as possible so that discharging efficiency of wash load could increase. d) In case of avoiding the setting of the siphon, irrigation aqueduct, irrigation inverted siphon, or drainage flume should be recommended in accordance with topography. 5. Discharging capability of wash load by drainage culvert appeared to depend hardly upon the diameter of the culvert, but greatly upon the location, specially near village, for there stones and dirts dumped may considerably be piled up. So, a counter plan for that is required.

• Proceedings of the Korea Society of Environmental Biology Conference
• /
• 2002.11a
• /
• pp.33-37
• /
• 2002

The present was performed to identify the effects of tributyltin (TBT) in the immature mice testes. 3-week-old male ICR mice were orally administrated on one time basis of TBT dose of O (Vehicle control, VC), 25 (TBT 25 mg/kg, T$_{25}$ ), 50 (TBT 50 mg/kg, T$_{50}$ ), 100 (TBT 100 mg/kg, T$_{100}$ ) mg/kg per each one. After 3 days the time treated of TBT, mice were sacrified and wighted body, testis, epididymis, seminal vesicle, vas deferens, and prostate. As the result of weighing, wights of each oragan and gonad index were tendency decresed in comparing groups of TBT treated with that (C) of unteated (p <0.05). As the result of examination of steroid hormones in the immature male mice, The concentrations of serum and intratesticular testosterone were significatly increased rather than the control group. But concentrations of estradiol were decresed objectly. A group of the highest change of concentrations of steroid hormones is T$_{100}$ . The high dose group, T$_{100}$ , was decreased all of concentrations of steroid hormones rather than those of T$^{25}$ . The result of observation with histological changes in testis showed a tendency for innercellular wall to increase damage and extinction in seminiferous tubles. As the result of investigation apoptotic cell numbers in the testis using teminal deoxy-nucleotidyl transferase -mediated dUTP-digoxygenin nick end-labeling immunohistochemical straia, The ratio of Apoptic cells significantly was incensed in depending on treatment of TBT does. In conclusion, these results shows that TBT triggers apoptosis on reproductive cell in testis and changes level of concentrations of steroid hormones in the immature male mice , as endocrine disruptors (EDs).

• Korean Journal of Food Science and Technology
• /
• v.15 no.4
• /
• pp.392-398
• /
• 1983

A pilot single-screw food extruder was constructed, and its mechanical properties and product characteristics were investigated by using corn grits. The screw rotational speed was varied and the changes in temperature profile of the barrel for the start-up period of operation were measured. The rate of heat generation for the start-up period was affected by the screw speed and feed rate. The screw speed resulted in a great influence on the estimated dough viscosity. The changes in the dough viscosity could indicate the on-set of termoplastic reaction in the barrel. The expansion ratio during the start-up period mainly depended on the barrel temperature and the degree of thermoplastic reaction in the barrel. The barrel temperatures for the gelatinization and burning of corn grits depended on the screw speed as well as the feed rate.