• Asian-Australasian Journal of Animal Sciences
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• 제8권5호
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• pp.463-470
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• 1995

An experiment was conducted to evaluate the effects of chromium picolinate on growth performance, nutrient utilizability, carcass composition, serum traits and in vitro protein synthesis of 3 day old Arbor Acres broiler chickens when dietary crude protein levels were varying in diets. Six replicates of eight chicks each (average initial weitht = 59.4 g) were randomly assigned to three levels (low, medium, high) of dietary crude protein at two levels of chromium (0, 200 ppb Cr/kg diet) as chromium picolinate. Six chicks/treatment were randomly chosen for analyses of carcass composition, six additional chicks/treatment were randomly chosen for analyses of serum components, and a chick/treatment was chosen for in vitro culture of liver tissue. Chromium picolinate did not affect feed intake, protein and fat utilizability, regradless of dietary crude protein level. But feed/gain ratio were more improved in groups fed the low protein diets added with chromium picolinate compared with groups fed the medium and high protein diets with chromium picolinate. Carcass fat tended to decrease whereas carcass protein tended to increase when added with chromium picolinate. Broilers fed diets with chromium picolinate exhibited lower serum triglyceride and nonesterified fatty acid concentrations than those fed without chromium picolinate (p < 0.05). Both secreted and retained proteins in cultured acinar cell were higher in groups fed diets with chromium picolinate than those fed diets without chromium picolinate (p < 0.05). It could be suggested that chromium picolinate was effective in improving weight gain and nutrient utilizability when dietary crude protein was low (p < 0.05), and also effective in manipulating carcass fat when dietary crude protein level was high (p < 0.05).

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2002년도 추계학술발표회
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• pp.148-151
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• 2002

To clarify of chemical contents for the Keumsan ginseng, two attempts have been done for two year: 2 year and 4 year-ginsengs coilected from the phyllite(Ph0 and granite area(Gr), and 1 year, 2 year and 3 year-ginsengs collected from the phyllite(Ph), shale(Sh) and granite (Gr), In the first attempt, the soil contents of the Ph are high in most of elements. In the comparisons between ages of the ginseng, regardless the area, most of the elements are high in the 4 year and these trends are distinctive in the granite area. In the comparisons of the areas, 2 year-ginsengs of the Ph show high contents in the most of the elements. Comparisons between upper part(leaf and branch) and root part of the ginsengs show that in the 2 year-ginseng, the Gr is high in the root part and the Ph is high in the upper part, while in the 4 year-ginseng, most of the elements are high in the root part. Comparisons between contents of soil and ginsengs show similar in crease and decrese trends. However, the 2 year-ginsengs show clear differences between two areas while in the 4 year-ginseng, differences between the Gr and Ph is not clear. It suggest that the ginsengs absorb eligible element contents with ages, despite of clear content differences of the different soils. In the second attempt, the Gr shows high W, Pb, Th, U, Sn and B contents wlile Ph and Sh show high Ni, Cr, Co, Sc, V, As, Cu and Zn contents. In the comparisons between ages of the ginseng, regardless the area, most of the elements are high in the 2 year, especially in the Gr and Ph. In the comparisons of the areas, 2 year-ginsengs of the Gr show low contents in the most of the elements, and the S1 show higher Ni, V, Th and U contents than the Ph. 3 year-ginsengs of the Gr show low contents in the most of the elements, while the S1 show high contents in the most of elements. relative to the Ph. Comparisons between upper part and root part of the ginsengs show that in the ginseng, regardless the ages, the upper part is high. Comparisons between contents of soil and ginsengs show similar increase and decrese trends, and soil is low than the ginsengs in the most of the elements

• 한국가축번식학회지
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• 제24권4호
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• pp.365-373
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• 2000

체세포 핵이식이 완료된 수정란을 70 volt 40 $\mu\textrm{s}$ 1회, 70 volt 40 $\mu\textrm{s}$ 2회, 180 volt 15 $\mu\textrm{s}$ 1 회 및 180 volt 30 $\mu\textrm{s}$ 1회의 전압을 이용하여 융합을 실시하였으며, 융합배지로는 mannitol 및 ZCFM 을 사용하였다. 70 volt 40 $\mu\textrm{s}$ 1회, 70 volt 40 $\mu\textrm{s}$ 2회 및 180 volt 15 $\mu\textrm{s}$ 1회의 전압을 이용하여 공핵 난구세포와 수핵란 세포질간에 융합을 유도한 결과, 융합율은 각각, 0.0%, 25.4% 및 58.1% 였으며, 배반포 발생율은 각각, 0.0%, 13.3% 및 36.1 % 였다. 180 volt 15 $\mu\textrm{s}$ 1회의 전압을 이용하였을 때 융합율 및 배반포 발생율이 유의적으로 높았다 (P＜0.05). 180 volt 15 $\mu\textrm{s}$ 및 30 $\mu\textrm{s}$ 1회의 전압을 이용하여 공핵 태아 섬유아세포와 수핵란 세포질간에 융합을 유도한 결과, 융합율은 각각,4 5.7% 및 63.2% 였으며, 배반포 발생율은 각각, 24.3% 및 25.0% 였다. 융합율은 180 volt 30 $\mu\textrm{s}$ 1회의 전압을 이용하였을 때 유의적으로 높았으나, 배반포 발생율에 있어서는 차이를 나타내지 않았다(P＜0.05). Mannitol 및 ZCFM 을 이용하여 융합을 실시한 결과, 융합율은 각각, 71.2 % 및 65.8% 였고, 배반포 발생율은 각각, 37.8% 및 39.8% 였다. 융합배지간 융합율 및 발생율에 있어서는 유의적인 차이를 나타내지 않았다.