• Title/Summary/Keyword: '-go'

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Real-time Fluorescence Assay of DNA Polymerase Using a Graphene Oxide Platform (산화 그래핀 플랫폼을 이용한 DNA 중합효소의 실시간 형광에세이)

  • Gang, Jongback
    • Microbiology and Biotechnology Letters
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    • v.41 no.4
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    • pp.456-461
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    • 2013
  • Using the different adsorption properties of ssDNA and dsDNA to GO, this study used a real time and efficient fluorescence assay to detect the enzymatic activity of the Klenow fragment with the adsorbed DNA to GO. Results showed that adsorption of fluorescein-tagged ssDNA to GO resulted in fluorescence quenching and DNA was released from GO by adding complementary DNA. In addition, fluorescence restoration was increased through a polymerization reaction by the Klenow fragment in the presence of a fluorescein-attached template, GO, and primer. Gel electrophoresis was conducted to confirm the hybridization and DNA polymerization reactions on GO.

Synthesis and characterization of polybenzoxazole/graphene oxide composites via in situ polymerization

  • Lim, Jun;Kim, Min-Cheol;Goh, Munju;Yeo, Hyeounk;Shin, Dong Geun;Ku, Bon-Cheol;You, Nam-Ho
    • Carbon letters
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    • v.14 no.4
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    • pp.251-254
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    • 2013
  • In this study, poly(amic acid) was prepared via a polycondensation reaction of 3,3'-dihydroxybenzidine and pyromellitic dianhydride in an N-methyl-2-pyrrolidone solution; reduced graphene oxide/polybenzoxazole (r-GO/PBO) composite films, which significantly increased the electrical conductivity, were successfully fabricated. GO was prepared from graphite using Brodie's method. The GO was used as nanofillers for the preparation of r-GO/PBO composites through an in situ polymerization. The addition of 50 wt% GO led to a significant increase in the electrical conductivity of the composite films by more than sixteen orders of magnitude compared with that of pure PBO films as a result of the electrical percolation networks in the r-GO during the thermal treatment at various temperatures within the films.

Effect of Alii Macrostemi Bulbus on Cultured Mouse Osteoblasts Damaged by Reactive Oxygen Species (활성산소로 손상된 골모세포에 대한 해백의 영향)

  • Son Il Hong;Yang Hyun Woong;Lee Jai Kyoo;Lee Kang Chang
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.3
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    • pp.787-790
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    • 2003
  • It has been demonstrated that oxidative stress of reactive oxygen species(ROS) may be a causative factor in the pathogenesis of bony disorder. The purpose of this study was to evaluate the oxidative stress of glucose oxidase(GO) in the cultured mouse osteoblasts and the protective effect of Alii Macrostemi Bulbus(AMB) on ROS-induced osteotoxicity. Toxic effect of GO and protective effect of AMB were carried out by colorimetric assay. 20mU/mi GO decreased cell viability dose-dependently, and AMB increased cell viability against GO-induced cytotoxicity in these cultures. From above the results. GO has toxic effect, and AMB is very effective on GO-induced osteotoxicity in cultured osteoblasts of neonatal mouse.

Pharmacognostical Studies oh the 'Ha Go Cho' ("하고초(夏枯草)"의 생약학적 연구)

  • Whang, Myung-Suk;Cho, Chang-Hee;Park, Jong-Hee
    • Korean Journal of Pharmacognosy
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    • v.31 no.2
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    • pp.209-215
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    • 2000
  • 'Ha Go Cho (夏枯草)' is one of the Chinese crude drugs used mainly as a diuretic. With regard to the botanical origin of 'Ha Go Cho', it was reported by Su et al. that those from China were originated from the fruited spica of Prunella vulgaris L. of Labiatae. It was, however, for the herba or spica of Prunella vulgaris L. var. lilacina Nakai from Korea. According to survey of markets in Korea, most of the materials collected in the markets seemed to be originated from Prunella plant, while some seemed to be Thesium plant of Santalaceae. To clarify the botanical origin of 'Ha Go Cho', the anatomical characteristics of Prunella vulgaris L. var. lilacina Nakai and Thesium chinense Turcz. were studied. As a result, it was clarified that some 'Ha Go Cho' from Korea were the herba or spica of Prunella vulgaris var. lilacina, whereas some others were the herba of Thesium chinense.

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자력복구 적응 채널등화기를 위한 Run and Go 알고리즘 (Run and Go Algorithm for Blind Equalization)

  • Chung, Won-Zoo
    • Journal of IKEEE
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    • v.10 no.1 s.18
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    • pp.62-68
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    • 2006
  • In this paper, we propose an adaptation strategy for blind equalizers, which combines a blind algorithm based on high order statistics and the decision directed LMS algorithm. In contrast to 'Stop-and-Go' algorithm, where adaptation is stopped for unreliable signals, the proposed algorithm applies high order statistics (HOS) blind algorithm to the unreliable signals and applies DD-LMS for the reliable signals. The proposed algorithm, named 'Run-and-Go' algorithm, inherits minimum MSE performance of DD-LMS and acquisition ability of blind algorithms. Furthermore, by updating the reliable signal region according to signal quality in each iteration, the convergence speed and acquisition ability is further improved.

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Preparation of Graphene Based PdOx and CuOx/MnOx Nanocomposites and Their Catalytic Applications in C-C Coupling and CH3SH Decomposition Reactions

  • Lee, Gyeong-Hun;Park, Jun-Beom
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.175.2-175.2
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    • 2014
  • Graphene (G) has been modified with palladium, copper, and manganese oxide nanoparticles (NPs), and their catalytic applications have been studied in C-C coupling reactions and methylmercaptan (CH3SH) decomposition reactions. In this research, graphite oxide (GO) sheets were exfoliated and oxidized from graphite powder and impregnated with metal precursors including Pd2+, Cu2+, and Mn2+. The thermal treatments of the metal impregnated GO in preferred gas environments produced Pd NPs on graphene (Pd/G), PdO NPs on GO (PdO/GO), and CuOx and MnOx NPs on graphene (CuOx/MnOx/G). In case of Pd/G and PdO/GO, the TEM images show that, although the mean size of the Pd NPs changed significantly before and after the C-C coupling reaction, that of the PdO NPs didn't, implying that the PdO/GO was superior to Pd/G in terms of the recyclability. Also, we demonstrate that the CuOx/MnOx/G exerts the excellent catalytic efficiency in CH3SH decomposition reaction comparing with conventional catalysts. The chemical and electronic structural changes were investigated using XRD and XPS.

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An Improved GoGOP Structure for Multi-view Video Coding in H.264/AVC (H.264/AVC에서 다시점 비디오 부호화를 위한 향상된 GoGOP 구조)

  • Shin, Kwang-Mu;Lee, Seo-Young;Kim, Sung-Min;Chung, Ki-Dong
    • Journal of KIISE:Computing Practices and Letters
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    • v.14 no.3
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    • pp.271-275
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    • 2008
  • Corresponding recent continuous development of multimedia technology with improved desire of using various contents, a new realistic feeling media technology is being created. Of them all, multi-view video is being researched actively as foundation technology of 3D TV, free-view point video etc. But encoding time and bit-rate are increased as view numbers are increased. In this paper we propose improved GoGOP structure to enhance the coding efficiency of multi-view video by applying methods which are using techniques such as Key frame position adjustment, dynamically changing the number of I frame and B frame. As experimental results, technique proposed in this paper reduces bit-rate having similar PSNR value compared with Anchor structure.

Effect of Benincasae Semen on Glucose Oxidase in Cultured Mouse Spinal Motor Neurons (척수운동신경세포에 있어서 Glucose Oxidase의 독성에 대한 동과의 영향)

  • Choi Yu Sun;Yang Hyun Woong;Lee Joung Hwa;Lee Kang Chang
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.2
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    • pp.457-460
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    • 2003
  • It has been suggested that oxidative stress of reactive oxygen species(ROS) may play an important role in the pathogenesis of neurological disorder. The aim of this study was to elucidate the oxidative stress of glucose oxidase(GO) in the cultured mouse spinal motor neurons and the preventing effect of Benincasae Semen(BS) on ROS-induced neurotoxicity. Cytotoxic effect of GO and protective effect of BS were performed by MTT assay. 30mU/ml GO decreased cell viability in dose-and time-dependent mannner, and BS diminished GO-induced neurotoxicity in these cultures. From above the results, ROS such as GO has toxic effect, and herb extract of BS is very effective against GO-induced neurotoxicity in cultured spinal motor neurons of neonatal mouse.

The Effect of sGO Content in sPEEK/sGO Composite Membrane for Unitized Regenerative Fuel Cell (일체형 재생연료전지 적용을 위한 sGO 함량 변화에 따른 sGO/sPEEK 복합막의 특성 평가)

  • Jung, Ho-Young;Kim, Min-Woo;Lim, Ji-Hun;Choi, Jin H.;Roh, Sung-Hee
    • KEPCO Journal on Electric Power and Energy
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    • v.2 no.1
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    • pp.127-131
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    • 2016
  • Polymer electrolyte membrane for unitized regenerative fuel cells requires high proton conductivity, high dimensional stability, low permeability, and low cost. However, DuPont's Nafion which is a commercial polymer electrolyte membrane has high permeability, high cost, and decreasing proton conductivity and dimensional stability over $80^{\circ}C$. To address these problems, sulfonated poly ether ether ketone (sPEEK) which is a low cost hydrocarbon polymer is selected as matrix polymer for the preparation of polymer electrolyte membrane. In addition, composite membrane with improved proton conductivity and dimensional stability is prepared by introducing sulfonated graphene oxide (sGO). The fundamental properties of polymer electrolyte membranes are analyzed by investigating membrane's water content, dimensional stability, proton conductivity, and morphology. The cell test is conducted to consider the possibility of application of sPEEK/sGO composite membrane for an unitized regenerative fuel cell.

The modified adaptive blind stop-and-go algorithm for application to multichannel environment (다중 채널 환경에 적용을 위한 변형된 적응 블라인드 stop-and-go 알고리듬)

  • 정길호;김주상;변윤식
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.21 no.4
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    • pp.884-892
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    • 1996
  • An adaptive blind equalizer is used to combat the distortions caused by a nonideal channel without resorting to a training sequence, given the received signal and statistical information of the transmitted signal. Incidentally, a multipath channel may result in a fade which produces intersymbol interference in the received signal. Therefore, a new type of algorithm which can compenste the effects of this fade is required in the multipath channel environment. In this paper, a modified form of adaptive blind equalization algorithm using stop-and-go algorithm for multichannel system is proposed. It is demonstrated via computer simulations that the performance of the proposed multichannel stop-and-go algorithm is much better than that of the conventional multichannel algorithms.

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