• 제목/요약/키워드: '-go'

Search Result 12,309, Processing Time 0.038 seconds

Investigation of Zooplankton Communities in Streams in Northern Gyeonggi-do Province (경기북부 주요 하천 내 동물플랑크톤 군집특성 조사 연구)

  • Go, Soon-Mi;Im, Heung-Bin;Jung, Eun-Hee;Kim, Tae-Yuel;Kim, Jae-Kwang;Choi, Jeong-In;Lee, Ho-Jung;Oh, Jo-Gyo
    • Journal of Environmental Health Sciences
    • /
    • v.45 no.5
    • /
    • pp.426-433
    • /
    • 2019
  • Objectives: Zooplankton communities play important roles in aquatic ecosystems as secondary producers that graze on phytoplankton and in turn are preyed upon by planktivorous and juvenile fish. They can shift their distribution, species composition, and abundance in response to environmental changes. Therefore zooplankton communities are important for understanding the energy flow in aquatic ecosystems and can be valuable indicators of environmental conditions. However, zooplankton in streams are still not well-studied, especially in northern Gyeonggi-do Province. This study aims to investigate the zooplankton communities in major streams in northern Gyeonggi-do Province. Methods: Zooplankton is important in the nutrient cycle and energy flow of aquatic ecosystems. Therefore, we surveyed zooplankton and measured temperature, DO, BOD, COD, T-N, T-P, and Chl-a in major streams (Sincheon, Gongneungcheon, Wangsukcheon, and Gapyeongcheon Streams) and stagnant water (Gomoji Reservoir). Results: The water quality in Gapyeongcheon Stream was the highest grade, while that of Gomoji Reservoir was mesoeutrophic and eutrophic during the research period. In the zooplankton community, Nauplius, Rotaria, and Monostyla spp. were dominant in Sincheon, Gongneungcheon, and Wangsukcheon Streams, and the dominance index was also high. In the case of Gapyeongcheon Stream, it was found that water quality and aquatic ecosystem health were good, and the lowest dominance index reflected this. In Gomoji Reservoir, Polyarthra spp., Nauplius, and Bosmina longirostris, which can be easily observed as eutrophication progresses, showed a high dominance rate. Therefore, it is necessary to monitor the progress of eutrophication in further research. Conclusions: We collected data on the zooplankton communities in streams and investigated their characteristics. As a result, specific species were found to be dominant at each survey sites and some of them are known to be observed as eutrophication progresses. Therefore, we should investigate the zooplankton community of streams around us and apply ecological stream management.

Transcriptome and Flower Color Related Gene Analysis in Angelica gigas Nakai Using RNA-Seq (RNA-seq을 이용한 참당귀의 전사체 분석과 꽃 색 관련 유전자 분석)

  • Kim, Nam Su;Jung, Dae Hui;Park, Hong Woo;Park, Yun mi;Jeon, Kwon Seok;Kim, Mahn Jo
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2019.10a
    • /
    • pp.73-73
    • /
    • 2019
  • Angelica gigas Nakai (Korean danggui), a member of the Umbelliferae family, is a Korean traditional medicinal plant whose roots have been used for treating gynecological diseases. Transcriptomics is the study of the transcriptome, which is the complete set of RNA transcripts that are produced by the genome, using high-throughput methods, such as microarray analysis. In this study, transcriptome analysis of A.gigas Nakai was carried out. Transcriptome sequencing and assembly was carried out by using Illumina Hiseq 2500, Velvet and Oases. A total of 109,591,555 clean reads of A. gigas Nakai was obtained after trimming adaptors. The obtained reads were assembled with an average length of 1,154 bp, a maximum length of 13,166 bp, a minimum length of 200 pb, and N50 of 1,635 bp. Functional annotation and classification was performed using NCBI NR, InterprotScan, KOG, KEGG and GO. Candidate genes for phenylpropanoid biosynthesis were obtanied from A.gigas transcriptome and the genes and its proteins were confirmed through the NCBI homology BLAST searches, revealing high identity with other othologous genes and proteins from various plants pecies. In RNA sequencing analysis using an Illumina Next-Seq2500 sequencer, we identified a total 94,930 transcripts and annotated 71,281 transcripts, which provide basic information for further research in A.gigas Nakai. Our transcriptome data reveal that several differentially expressed genes related to flower color in A.gigas Nakai. The results of this research provide comprehensive information on the A.gigas Nakai genome and enhance our understanding of the flower color related gene pathways in this plant.

  • PDF

Recombinant Production and Antimicrobial Activity of an Antimicrobial Model Peptide (Uu-ilys-CF) Derived from Spoon Worm Lysozyme, Uu-ilys (개불 라이소자임 유래 항균성 모델 펩타이드(Uu-ilys-CF)의 재조합 단백질 생산 및 항균 활성)

  • Oh, Hye Young;Go, Hye-Jin;Park, Nam Gyu
    • Journal of Life Science
    • /
    • v.31 no.1
    • /
    • pp.83-89
    • /
    • 2021
  • Uu-ilys, an i-type lysozyme from spoon worm (Urechis unicinctus), is an innate immune factor that plays an important role in the defense against pathogens. It also possesses non-enzymatic antibacterial activity. Thus, there is a possibility to develop an antimicrobial model peptide from Uu-ilys. In this study, we report the design, production, and antibacterial activity of an Uu-ilys analog that exhibits antibacterial activity. The Uu-ilys structure was fragmented according to its secondary structures to predict the regions with antimicrobial activity using antimicrobial peptide (AMP) prediction tools from different AMP databases. A peptide containing the C-terminal fragment was predicted to exert antimicrobial activity. The chosen fragment was designated as an Uu-ilys analog containing the C-terminal fragment, Uu-ilys-CF. To examine the possibility of developing an AMP using the sequence of Uu-ilys-CF, recombinant fusion protein (TrxA-Uu-ilys-CF) was produced in an expression system that was heterologous. The produced fusion protein was cleaved after methionine leaving Uu-ilys-CF free from the fusion protein. This was then isolated through high performance liquid chromatography and reverse phase column, CapCell-Pak C18. The antibacterial activity of Uu-ilys-CF against different microbial strains (four gram-positive, six gram-negative, and one fungal strain) were assessed through the ultrasensitive radial diffusion assay (URDA). Among the bacterial strains tested, Salmonella enterica was the most susceptible. While the fungal strain tested was not susceptible to Uu-ilys-CF, broad spectrum antibacterial activity was observed.

Anti-obesity Effect of the Flavonoid Rich Fraction from Mulberry Leaf Extract (뽕잎 추출물 기원 Flavonoid Rich Fraction의 항비만효과)

  • Go, Eun Ji;Ryu, Byung Ryeol;Yang, Su Jin;Baek, Jong Suep;Ryu, Su Ji;Kim, Hyun Bok;Lim, Jung Dae
    • Korean Journal of Medicinal Crop Science
    • /
    • v.28 no.6
    • /
    • pp.395-411
    • /
    • 2020
  • Background: This study investigated the anti-obesity effect of the flavonoid rich fraction (FRF) and its constituent, rutin obtained from the leaf of Morus alba L., on the lipid accumulation mechanism in 3T3-L1 adipocyte and C57BL/6 mouse models. Methods and Results: In Oil Red O staining, FRF (1,000 ㎍/㎖) treatments showed inhibition rate of 35.39% in lipid accumulation compared to that in the control. AdipoRedTM assay indicated that the triglyceride content in 3T3-L1 adipocytes treated with FRF (1,000 ㎍/㎖) was reduced to 23.22%, and free glycerol content was increased to 106.04% that of the control. FRF and its major constituent, rutin affected mRNA gene expression. Rutin contributed to the inhibition of Sterol regulatory element binding protein-1c (SREBP-1c) gene expression, and inhibited the transcription factors SREBP-1c, peroxisome proliferator-activated receptor gamma (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). In addition, the effect of FRF administration on obesity development in C57BL/6 mice fed high-fat diet (HFD) was investigated. FRF suppressed weight gain, and reduced liver triglyceride and leptin secretion. FRF exerted potential anti-inflammatory effects by improving insulin resistance and adiponectin levels, and could thus be used to help counteract obesity. The mRNA expressions of PPAR-γ, FAS, ACC, and CPT-1 were determined in liver tissue. Quantitative real-time PCR analysis was also performed to evaluate the expression of IL-1β, IL-6, and TNF-α in epididymal adipose tissue. Compared to the control group, mice fed the HFD showed the up-regulation in PPAR-γ, FAS, IL-6, and TNF-α genes, and down-regulation in CPT1 gene expression. FRF treatement markedly reduced the expression of PPAR-γ, FAS, IL-6, and TNF-α compared to those in HFD control, whereas increased the expression level of CPT1. Conclusions: These results suggest that the FRF and its major active constituent, rutin, can be used as effective anti-obesity agents.

Analysis of the Correlation Between Kidney Function Indicators and Kidney Size According Age Groups in Ultrasonography (신장 초음파 검사에서 연령대에 따른 신장 기능 지표와 신장 크기 간의 상관관계 분석)

  • Go, Ryo-won;Seoung, Youl-Hun
    • Journal of the Korean Society of Radiology
    • /
    • v.14 no.7
    • /
    • pp.871-879
    • /
    • 2020
  • The purpose of this study was to analysis the correlation between kidneys function indicators and these size in ultrasonography. A total of 170 (male:86, female:84) patients of sex and age groups were examined by abdominal ultrasonography. The patients classified as those in their 20's, 30's, 40's, and over 50's. We measured the length, width, and cross-sectional height of the kidneys twice. At this time, the length of these were measured from the maximum upper to the maximum lower pole and the widest width in the same ultrasonography was measured to obtain the cross-sectional area. Other relevant indicators included body surface area, serum creatinine, glomerular filtration rate (GFR), MDRD (Modification of diet in renal disease) and C-G (Cockcroft-Gault). Significant comparisons of differences between relevant factors by age groups and sex were conducted with a one-way distribution analysis. Correlation analysis was also performed between relevant factors by using Pearson and Spearman correlation coefficient. It was defined as meaningful when the p-value was less than 0.05. As a result, the length, the width, and the cross-sectional area of kidneys were correlated with GFR, C-G, MDRD. Therefore, it is expected that the accuracy of diagnosis of kidneys disease will be increased if the relevant indicators are evaluated together rather than measuring only length of these in ultrasonography.

Performance and Charging-Discharging Behavior of AGM Lead Acid Battery according to the Improvement of Bonding between Active Material/Substrate using Sand-Blasting Method (Sand-Blasting법을 이용한 활물질/기판간 결합력 향상에 따른 AGM 연축전지의 성능 및 충방전 거동)

  • Kim, Sung Joon;Lim, Tae Seop;Kim, Bong-Gu;Son, Jeong Hun;Jung, Yeon Gil
    • Korean Journal of Materials Research
    • /
    • v.31 no.2
    • /
    • pp.75-83
    • /
    • 2021
  • To cope with automobile exhaust gas regulations, ISG (Idling Stop & Go) and charging control systems are applied to HEVs (Hybrid Electric Vehicle) for the purpose of improving fuel economy. These systems require quick charge/discharge performance at high current. To satisfy this characteristic, improvement of the positive electrode plate is studied to improve the charge/discharge process and performance of AGM(Absorbent Glass Mat) lead-acid batteries applied to ISG automotive systems. The bonding between grid and A.M (Active Material) can be improved by applying the Sand-Blasting method to provide roughness to the surface of the positive grid. When the Sand-Blasting method is applied with conditions of ball speed 1,000 rpm and conveyor speed 5 M/min, ideal bonding is achieved between grid and A.M. The positive plate of each condition is applied to the AGM LAB (Absorbent Glass Mat Lead Acid Battery); then, the performance and ISG life characteristics are tested by the vehicle battery test method. In CCA, which evaluates the starting performance at -18 ℃ and 30 ℃ with high current, the advanced AGM LAB improves about 25 %. At 0 ℃ CA (Charge Acceptance), the initial charging current of the advanced AGM LAB increases about 25 %. Improving the bonding between the grid and A.M. by roughening the grid surface improves the flow of current and lowers the resistance, which is considered to have a significant effect on the high current charging/discharging area. In a Standard of Battery Association of Japan (SBA) S0101 test, after 300 A discharge, the voltage of the advanced AGM LAB with the Sand-Blasting method grid was 0.059 V higher than that of untreated grid. As the cycle progresses, the gap widens to 0.13 V at the point of 10,800 cycles. As the bonding between grid and A.M. increases through the Sand Blasting method, the slope of the discharge voltage declines gradually as the cycle progresses, showing excellent battery life characteristics. It is believed that system will exhibit excellent characteristics in the vehicle environment of the ISG system, in which charge/discharge occurs over a short time.

A Study on the Improvement of Local Education Autonomy System

  • Park, Jong-Ryeol;Noe, Sang-Ouk
    • Journal of the Korea Society of Computer and Information
    • /
    • v.26 no.2
    • /
    • pp.141-150
    • /
    • 2021
  • Article 117, Paragraph 1 of the 「Constitution」 states that "Local governments may enact provisions relating to local autonomy, within the limit of Acts and subordinate statutes". It restricts the enactment of effective self-government laws. The fundamental problem-solving is securing the right to self-governing legislation through constitutional amendment. Therefore, it must be revised to "Local governments can make regulations on self-government to the extent that they handle resident welfare affairs in accordance with the subsidiarity and do not violate the law." In the long-term perspective, the current education council problem, which is contrary to the constitution, has to be revived as a constitutional independent education committee system, and the voting agency and the executive agency must go together and education councilors must have about 10 years of experience in education and education administration. The current superintendent's election system is of great significance in establishing democratic legitimacy by ensuring residents' right to vote and securing a superintendent's representation of residents. It hasn't been long since the system was implemented, but there are some side effects and it is argued that the election system should be replaced by the appointment by the head of the local government, the running mate system or the joint registration system. However it is thought that it is necessary to minimize and supplement the side effects rather than fixing the system as it violates the Constitution of the local education autonomy system.

Usability of CPR Training System based on Extended Reality (확장현실 기반의 심폐소생술 교육 시스템의 사용성 평가)

  • Lee, Youngho;Kim, Sun Kyung;Choi, Jongmyung;Park, Gun Woo;Go, Younghye
    • Journal of Internet of Things and Convergence
    • /
    • v.8 no.6
    • /
    • pp.115-122
    • /
    • 2022
  • Recently, the importance of CPR training for the layperson has been emphasized to improve the survival rate of out-of-hospital cardiac arrest patients. An accurate and realistic training strategy is required for the CPR training effect for laypersons. In this study, we develop an extended reality (XR) based CPR training system and evaluate its usability. The XR based CPR training system consisted of three applications. First, a 3D heart anatomy image registered to the manikin is transmitted to the smart glasses to guide the chest compression point. The second application provides visual and auditory information about the CPR process through smart glasses. At the same time, the smartwatch sends a vibration notification to guide the compression rate. The 'Add-on-kit' is a device that detects the depth and speed of chest compression via sensors installed on the manikin and sends immediate feedback to the smartphone. One hundred laypersons who participated in this study agreed that the XR based CPR training system has realism and effectiveness. XR based registration technology will contribute to improving the efficiency of CPR training by enhancing realism, immersion, and self-directed learning.

The Effect of Oryzalin on Growth and Gravitropism in Arabidopsis Roots (Oryzalin이 애기장대 뿌리 생장과 굴중성 반응에 미치는 작용)

  • Go, Jin Gyu;Park, Sun Ill;Kim, Soon Young
    • Journal of Life Science
    • /
    • v.31 no.2
    • /
    • pp.109-114
    • /
    • 2021
  • Oryzalin is a dinitroaniline herbicide that has been known to disrupt microtubules. Microtubules and microfilaments are components of cytoskeletons that are implicated in plant cell growth, which requires the synthesis of cellulose when cell walls elongate. In addition, microtubules are also involved in the sedimentation of statoliths, which regulate the perception of gravity in the columella cells of root tips. In this study, we investigated the effect of oryzalin on the growth and gravitropic response of Arabidopsis roots. The role of ethylene in oryzalin's effect was also examined using these roots. Treatment of oryzalin at a concentration of 10-4 M completely inhibited the roots' growth and gravitropic response. At a concentration of 10-6 M oryzalin, root growth was inhibited by 47% at 8 hr when compared to control. Gravitropic response was inhibited by about 38% compared to control in roots treated with 10-6 M oryzalin for 4 hr. To understand the role of oryzalin in the regulation of root growth and gravitropic response, we measured ethylene production in root segments treated with oryzalin. It was found that the addition of oryzalin stimulated ethylene production through the activation of ACC oxidase and ACC synthase genes, which are key components in the synthesis of ethylene. From these findings, it can be inferred that oryzalin inhibits the growth and gravitropic response of Arabidopsis roots by stimulating ethylene production. The increased ethylene alters the arrangement of the microtubules, which eventually interferes with the growth of the cell wall.

Development of a Personal Clothing Recommendation System that Reflects Individual Temperature Sensitivity (개인별 체감 온도를 반영한 개인 소장 의류 추천 시스템 개발)

  • Jeong, Byeong-Hui;Kim, Woo-Seok;Lee, Sang-Yong
    • Journal of Digital Convergence
    • /
    • v.19 no.2
    • /
    • pp.357-363
    • /
    • 2021
  • In general, people choose clothes to wear when they go out, referring to real-time weather and temperature. However, it is difficult for an individual to use real-time weather information and his or her temperature sensitivity information to choose the right clothes from among the clothes he or she owns. Existing clothing recommendation systems developed to help with these problems have problems recommending clothes that are not clearly set in the clothing category and are not in the possession of the user. In addition, user-specific temperature sensitivity is not taken into account, resulting in inappropriate clothing recommendations for users. To solve these problems, this study developed a system that determines and registers clothing categories for the clothing owned by the user, and recommends customized clothing for each user by considering temperature sensitivity and real-time weather information. In the case of weather information, not only weather information such as temperature and wind direction, but also clothes based on temperature sensitivity were recommended based on the calculation of temperature sensitivities. A satisfaction survey of 65 university students was conducted to assess the system. As a result, 80% of the respondents were satisfied with the recommended clothing, indicating that the satisfaction of the system was good. Therefore, it is expected that this system will be highly utilized in real life as it will be recommended based on clothes owned by individuals, reflecting individual temperature sensitivity.