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Ethanol Extract of Glycyrrhiza uralensis Protects Against Oxidative Stress-induced DNA Damage and Apoptosis in Retinal Pigment Epithelial Cells (망막색소상피세포에서 감초 추출물의 산화적 스트레스에 의한 DNA 손상 및 apoptosis 유발의 차단 효과)

  • Kim, So Young;Kim, Jeong-Hwan;Kim, Sung Ok;Park, Seh-Kwang;Jeong, Ji-Won;Kim, Mi-Young;Lee, Hyesook;Cheong, JaeHun;Choi, Yung Hyun
    • Journal of Life Science
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    • v.29 no.11
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    • pp.1273-1280
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    • 2019
  • Age-related macular degeneration (AMD) is one of the leading causes of blindness in the elderly population, and damage to retinal pigment epithelial (RPE) cells due to oxidative stress contributes to the development of AMD. Glycyrrhiza uralensis Fischer is one of the most widely used herbal medicines for the treatment of various diseases in Asian countries. Although recent studies indicated that treatment with G. uralensis can protect cells from oxidative stress, its mechanisms in RPE cells remain unknown. We evaluated the effect of a G. uralensis ethanol extract (GU) on $H_2O_2$-induced oxidative injury in ARPE-19 RPE cells. The GU pretreatment attenuated reactive oxygen species (ROS) generation induced by $H_2O_2$, which was associated with induced expression of nuclear factor erythroid-derived-2-like 2 (Nrf2) and heme oxygenase-1 (HO-1). GU also suppressed $H_2O_2$-induced DNA damage and mitochondrial dysfunction. The inhibitory effect of GU on $H_2O_2$-induced apoptosis was associated with the protection of caspase-3 activation. Overall, GU appeared to protect RPE cells from oxidative injury by inhibiting DNA damage and reducing apoptosis. Further studies are needed to determine the regulation of Nrf2-mediated HO-1 expression, but our results suggest the possibility of using GU to reduce the risk of AMD.

Assessment of the Effect of Dimethyl Ether (DME) Combustion on Lettuce and Chinese Cabbage Growth in Greenhouse (온실에서 상추와 배추를 이용한 DME 원료 난방 효율분석)

  • Basak, Jayanta Kumar;Qasim, Waqas;Khan, Fawad;Okyere, Frank Gyan;Lee, Yongjin;Arulmozhi, Elanchezhian;Park, Jihoon;Cho, Wonjun;Kim, Hyeon Tae
    • Journal of Bio-Environment Control
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    • v.28 no.4
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    • pp.293-301
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    • 2019
  • The experiment was conducted to determine the performance of DME combustion gas when used as a fuel for DME burner for raising temperature and $CO_2$ concentration in greenhouse and also to examine its effects on chlorophyll content, and fresh and dry weight of lettuce and Chinese cabbage. DME-1 and DME-2 treatments consisted of average DME flow quantity in duct were $17.4m^3min^{-1}$ and $10.2m^3min^{-1}$ respectively to greenhouse-1 and greenhouse-2 and no DME gas was supplied to greenhouse-3 which was left as control (DME-3). DME supply times were $0.5hr\;day^{-1}$, $1hr\;day^{-1}$, $1:30hrs\;day^{-1}$ and $2hrs\;day^{-1}$ on week 1, 2, 3, and 4 respectively. Chlorophyll content and fresh and dry weight of lettuce and Chinese cabbage were measured for each treatment and analyzed through analysis of variance with a significance level of P<0.05. The result of the study showed that $CO_2$ concentration increased up to 265% and 174% and the level of temperature elevated $4.8^{\circ}C$ and $3.1^{\circ}C$ in greenhouse-1 and 2, respectively as compared to greenhouse-3 due to application of DME combustion gas. Although, the same crop management practices were provided in greenhouse-1, 2 and 3 at a same rate, the highest change (p<0.05) of chlorophyll content, fresh weight and dry weight were found from the DME-1 treatment, followed by DME-2. As a result, DME combustion gas that raised the level of temperature and $CO_2$ concentration in the greenhouse-1 and greenhouse-2, might have an effect on growth of lettuce and Chinese cabbage. At end of experiment, the highest fresh and dry weight of lettuce and Chinese cabbage were measured in greenhouse-1 and followed by greenhouse-2. Similarly chlorophyll content of greenhouse-1 and greenhouse-2 were more compared to greenhouse-3. In general, DME was not producing any harmful gas during its combustion period, therefore it can be used as an alternative to conventional fuel such as diesel and liquefied petroleum gas (LPG) for both heating and $CO_2$ supply in winter season. Moreover, endorsed quantify of DME combustion gas for a specified crop can be applied to greenhouse to improve the plant growth and enhance yield.

The Effect of the Surfactant on the Migration and Distribution of Immiscible Fluids in Pore Network (계면활성제가 공극 구조 내 비혼성 유체의 거동과 분포에 미치는 영향)

  • Park, Gyuryeong;Kim, Seon-Ok;Wang, Sookyun
    • Economic and Environmental Geology
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    • v.54 no.1
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    • pp.105-115
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    • 2021
  • The geological CO2 sequestration in underground geological formation such as deep saline aquifers and depleted hydrocarbon reservoirs is one of the most promising options for reducing the atmospheric CO2 emissions. The process in geological CO2 sequestration involves injection of supercritical CO2 (scCO2) into porous media saturated with pore water and initiates CO2 flooding with immiscible displacement. The CO2 migration and distribution, and, consequently, the displacement efficiency is governed by the interaction of fluids. Especially, the viscous force and capillary force are controlled by geological formation conditions and injection conditions. This study aimed to estimate the effects of surfactant on interfacial tension between the immiscible fluids, scCO2 and porewater, under high pressure and high temperature conditions by using a pair of proxy fluids under standard conditions through pendant drop method. It also aimed to observe migration and distribution patterns of the immiscible fluids and estimate the effects of surfactant concentrations on the displacement efficiency of scCO2. Micromodel experiments were conducted by applying n-hexane and deionized water as proxy fluids for scCO2 and porewater. In order to quantitatively analyze the immiscible displacement phenomena by n-hexane injection in pore network, the images of migration and distribution pattern of the two fluids are acquired through a imaging system. The experimental results revealed that the addition of surfactants sharply reduces the interfacial tension between hexane and deionized water at low concentrations and approaches a constant value as the concentration increases. Also it was found that, by directly affecting the flow path of the flooding fluid at the pore scale in the porous medium, the surfactant showed the identical effect on the displacement efficiency of n-hexane at equilibrium state. The experimental observation results could provide important fundamental information on immiscible displacement of fluids in porous media and suggest the potential to improve the displacement efficiency of scCO2 by using surfactants.

A Development of a Mixed-Reality (MR) Education and Training System based on user Environment for Job Training for Radiation Workers in the Nondestructive Industry (비파괴산업 분야 방사선작업종사자 직장교육을 위한 사용자 환경 기반 혼합현실(MR) 교육훈련 시스템 개발)

  • Park, Hyong-Hu;Shim, Jae-Goo;Park, Jeong-kyu;Son, Jeong-Bong;Kwon, Soon-Mu
    • Journal of the Korean Society of Radiology
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    • v.15 no.1
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    • pp.45-54
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    • 2021
  • This study was written to create educational content in non-destructive fields based on Mixed Reality. Currently, in the field of radiation, there is almost no content for educational Mixed Reality-based educational content. And in the field of non-destructive inspection, the working environment is poor, the number of employees is often 10 or less for each manufacturer, and the educational infrastructure is not built. There is no practical training, only practical training and safety education to convey information. To solve this, it was decided to develop non-destructive worker education content based on Mixed Reality. This content was developed based on Microsoft's HoloLens 2 HMD device. It is manufactured based on the resolution of 1280 ⁎ 720, and the resolution is different for each device, and the Side is created by aligning the Left, Right, Bottom, and TOP positions of Anchor, and the large image affects the size of Atlas. The large volume like the wallpaper and the upper part was made by replacing it with UITexture. For UI Widget Wizard, I made Label, Buttom, ScrollView, and Sprite. In this study, it is possible to provide workers with realistic educational content, enable self-directed education, and educate with 3D stereoscopic images based on reality to provide interesting and immersive education. Through the images provided in Mixed Reality, the learner can directly operate things through the interaction between the real world and the Virtual Reality, and the learner's learning efficiency can be improved. In addition, mixed reality education can play a major role in non-face-to-face learning content in the corona era, where time and place are not disturbed.

Analysis of Major Factors related to the Generation of Fine Particulate Matter in Hanwoo Manure Composting Facilities (한우분뇨 퇴비화시설에서의 미세 입자상물질 발생 주요인자 분석)

  • Jeong, Kwang-Hwa;Park, Hoe-Man;Lee, Dong-Jun;Kim, Jung-Kon;Lee, Dong-Hyun;Kim, Da-Hye
    • Journal of the Korea Organic Resources Recycling Association
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    • v.28 no.4
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    • pp.53-68
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    • 2020
  • The concentrations of ammonia, hydrogen sulfide and fine dust were measured in the compost facility of a full-time Hanwoo breeding farms. The experiments were conducted in stack type composting facility(T1) and mechanical-stirred composting facilities(T2, T3). In the stack type composting facility, the highest temperature of compost pile was 46℃, and in the two mechanical-stirred composting facilities, it rose to 63℃ and 68℃, respectively. The concentrations of PM2.5 at T1, T2 were 15 ㎍/㎥ and 5~10 ㎍/㎥, respectively. And the concentration of PM2.5 at T3 was below 10 ㎍/㎥. The highest concentration of ammonia generated at T1 was 4 ppm, but no hydrogen sulfide was detected. The ammonia concentrations at T2 and T3 were 3 ppm and 4 ppm, respectively. However, hydrogen sulfide was not detected in both facilities. At T3, the ammonia concentration increased to 65 ppm at the point where the compost was stirred with a mechanical agitator. During composting period, the pH of the compost pile decreased from 9.06 to 8.94 and then increased to 9.14 as the composting period elapsed. The NaCl content of compost was 0.09% after composting process was complete. Moisture content of compost decreased from 65.9% to 62% as composting progressed. As composting proceeded, the content of volatile solids decreased from 65.6% to 64.7% and the content of TKN decreased from 1.327% to 1.095%.

Christian Education with the Socially Disadvantaged in and after the Covid-19 Pandemic (사회적 약자와 함께 하는 기독교교육)

  • Kim, Doil
    • Journal of Christian Education in Korea
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    • v.64
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    • pp.51-79
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    • 2020
  • This study was conducted to pursue Christian education with the socially underprivileged in the era of the Corona-19 pandemic. Corona-19 is a disaster which is caused, destroyed and exploited by human being. At the time of the indiscriminately spreading global pendemic, we must work together to overcome our selfish self-centeredness and make an attempt for everyone in need. It is a study on how humans can help each other survive in the era of Corona-19 and its post-corona. The problem is that there is too much discrimination between the state, race, and economic capacity, and in the end, the extreme discrimination of capitalism is appeared in society and across the country. There is no significant difference in the confirmation rate when Corona-19 infiltrates, but there is a big difference between those with and less in mortality. As a result, today's reality is that people who have a hard time living because they have less usually are far more vulnerable to blocking and defeating virus attacks. Unfortunately, this is the current situation. From the standpoint of a large discourse, attention is paid to climate change and ecological environment, and as a micro discourse, a number of societies who live with tremendous discrimination according to the gap between the rich and the poor (it is gender, race, disabled, nationality) that exist in almost all countries on the planet. We need attention to the weak. To this end, discourses on vaccine inequality, discourses on the needs of the disabled, discourses on different racial damages, discourses on polarization and dystopia, and discourses on educational inequality were treated as the reality faced by the socially underprivileged in the Corona 19 pandemic. To explore Christian education with the socially underprivileged, to explore ways of sharing, giving, and solidarity for win-win, discourse on inter-dependence and mutual responsibility of mankind, direct counter-measures for the socially underprivileged, and critical literacy education. He proposed a discourse on Korea, a discourse on Homo sapiens, which must return to being a part of creation, and finally a theology of friendship with the weak. Christian education based on Bible words must go forward in the era of the Corona 19 pandemic, hungry, naked, nowhere to go, sick, but dying because of being unable to get a remedy. He emphasized the need to establish a caring theology of friendship and pursue a life in which thought and practice harmonize. Thus, the paper proposed the spirit of Christian education not only doing something for the socially weak, but with the socially weak in the daily life.

Analysis of the Experiences and Perceptions of Teachers Participating in the Development of Content-Based Online Science Class Videos, and the Characteristics of the Developed Class Content (콘텐츠 활용형 온라인 과학 수업 동영상 개발에 참여한 교사들의 경험과 인식, 개발된 수업 콘텐츠의 특징 분석)

  • Shin, Jung Yun;Park, Sang Hee
    • Journal of The Korean Association For Science Education
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    • v.40 no.6
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    • pp.595-609
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    • 2020
  • The purpose of this study is to analyze the experiences of teachers who participated in the development of online science class videos in the context of covid-19, their perception of online science class, and the characteristics of the online science class content developed by teachers. A survey and interviews were conducted with ten elementary school teachers who made online science class videos themselves. Also the characteristics of the online science class were investigated by analyzing the online science class video produced by the participants. As a result, participants in the study recognized the lack of production time, difficulty in filming and editing, concerns over misconceptions, the problem of solving copyrights for existing materials, and the burden of external disclosure. Although it was a teacher who had experience producing online science class video contents, no research participants actively answered the merits of online science class. On the other hand, the study participants cited that the shortcomings of online science classes were that students had fewer opportunities for inquiry and lack of communication or interaction. In particular, these shortcomings were thought to have a great influence on the quality of online science classes, especially in making inquiry classes difficult. Some teachers took a negative view that online science classes could not completely replace face-to-face classes. However, if multiple teachers are presented with supplementary teaching activities that complement the content-based online teaching method, the method of combining online science classes and face-to-face classes is not. Through the analysis of the contents of the online science class, the introduction and arrangement steps of the online science class were similar to the process of the face-to-face science class, but the inquiry step and the conceptual explanation step showed a big difference from the face-to-face science class.

A Study on Antioxidant and Anti-inflammatory Effects Based on Analysis of Functional Components of Cornus officinalis Siebold & Zucc. (산수유의 채취 부위에 따른 기능 성분 분석과 항산화 및 항염증 효과에 관한 연구)

  • Hwangbo, Hyun;Jeung, Ji-Suk;Kim, Min Young;Ji, Seon Yeong;Yoon, Seonhye;Kim, Tae Hee;Kim, Sung Ok;Choi, Yung Hyun
    • Journal of Life Science
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    • v.31 no.3
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    • pp.287-297
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    • 2021
  • Cornus officinalis Siebold & Zucc. is traditionally used as an edible and medicinal plant in many countries in East Asia. Previous studies have shown the pharmacological potential of extracts and components of C. officinalis, but comparative analysis of the composition of the leaf, stem, and fruit extracts has been insufficient to date. In the present study, the content of active antioxidant and anti-inflammatory ingredients was verified in different C. officinalis parts (under-ripe sansuyu, ripe sansuyu, seed, leaf, stem, and dried sansuyu). One active component, morroniside, was high in fruit (under-ripe and ripe sansuyu), while loganin was high in fruit (under-ripe sansuyu) and cornin was high in seeds. Total polyphenol contents were highest in fruit (ripe sansuyu) and flavonoids were highest in leaves. DPPH radical scavenging activity was highest in leaves, followed by seeds and then ripe sansuyu extract. The anti-inflammatory efficacy of leaf extracts of C. officinalis (LCO) was further investigated by measuring their effects on levels of nitric oxide (NO) and the pro-inflammatory cytokines interleukin (IL)-1β and IL-6 in RAW 264.7 macrophages. Non-cytotoxic concentrations of LCO effectively decreased the lipopolysaccharide (LPS)-induced expression of inducible NO synthase, resulting in decreased NO production. LCO also significantly suppressed LPS-induced production and expression of IL-1β and IL-6. Taken together, the present findings suggest that C. officinalis leaves have potential as natural materials for the development of antioxidant and anti-inflammatory agents.

The review of characteristic for 'SUNBI'spirit, seen literati arts of confucian scholar -focused on literati paintings of confucian scholar for chosun dynasty- (유가 문인예술에 나타난 선비정신의 특질 -조선조 유가 문인의 문인화를 중심으로-)

  • Kwon, Yun Hee
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.1
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    • pp.117-133
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    • 2021
  • The art of the Confucian literati' was mainly centered on literati' painting. Literary paintings combined with poem-writing-painting are based on the literary characters and studies. This is usually based on the technique of Shensi(神似) caused by a hobby accomplishment, enjoying the chinese painting and cherishing with chinese painting. The Confucian literati' cultivated their own character and sublimated humanism to art based on studying. They sought the life of supremacy and supreme gentleness, and enjoyed life on the boundary of pleasure through art. The aim of the Confucian literati' arts lies in the pursuit of expressing the artist's inner world, spirit, and the combination of the Confucian and the Taoism, Because of literati's spirit based on learning, the Confucian literati' arts still exist. The aesthetic of Sunbi Spirit is mainly in the customs of Sunbi, the loyalty of Sunbi, the Silhak(實學) of Sunbi, and loving of the people of Sunbi. We can find honor and loyalty in the Sunbi spirit of the Confucian literati' of the Joseon Dynasty. In addition, it is also possible to observe the loyal troops, the hard work for the country, and the Pung-ryu with nature. In other words, the Sunbi honor, loyalty, loyal troops, pursuit of study and the Pung-ryu show the spirit of the Confucian literati' of Joseon Dynasty. The aesthetic of the Sunbi spirit is in Pung-ryu, loyalty, Silhak, loving of the people etc. The aesthetic of experience of art is mainly based on the aesthetic experience by emotional intelligence and the aesthetic experience according to the individual's inclination. The aesthetic sense actually shows Pung-ryu, loyalty, Silhak and love etc. We can see it in many of our literary paintings. Therefore, the Confucian literati' painting in Chosun Dynasty were the intentionality of the mind and the intentionality of the spare. Furthermore, it has directivity of expressing the artist's inner world, directivity of substance, so it is possible to see that the characteristics of the Sunbi spirit are diverse.

Distribution and Conservation plan of Vascular Plants in Outstanding Forest Wetlands in Jeonnam Area (전남권역 우량 산림습원의 관속식물상 변화 및 보전방안)

  • Lee, Jong-Won;Yun, Ho-Geun;Kang, Shin-Ho;An, Jong-Bin
    • Journal of Wetlands Research
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    • v.24 no.4
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    • pp.224-255
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    • 2022
  • This study was carried out to examine the changes in vascular flora in the first and second surveys targeting 32 outstanding forest wetlands in Jeonnam area including Gwangju metropolitan city, and based on this, establish a future monitoring system and effectively manage the excellent forest wetlands. The survey investigated the area seasonally every 4-5 years from 2015 to 2021. The change of flora of 32 excellent forest wetlands in Jeollanam-do increased from 103 families, 311 genera, 496 species, 16 subspecies, 38 varieties, 6 varieties, 556 taxa, to 111 families, 362 genera, 599 species, 16 subspecies, 51 varieties, 8 varieties, 674 taxa. Remarkable plants such as Red list and plants endemic to the Korean Peninsula increased also. However, Nepeta cataria and the like are exterminated locally, so an in situ and ex situ conservation strategy must be established. In the case of plants categorized according to wetland preference, the distribution ratio of such wetland plants slightly decreased and the distribution ratio of such terrestrial plants lightly increased for absolute wetland plants. It is necessary to prepare countermeasures for the terrestrialization of forest wetlands and to develop research techniques that can distinguish boundaries. In addition, the forest wetland should be systematically managed and supervised by designating it as a Forest Genetic Resources Reserve that can effectively conserve it.