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Studies on the Hydrolysis of the Waste wood of Cortinellus edodes with Trichoderma viride Cellulase (표고재배폐재(栽培廢材)의 당화(糖化)에 관(關)한 연구(硏究))

  • Min, Du Sik
    • Journal of Korean Society of Forest Science
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    • v.43 no.1
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    • pp.31-34
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    • 1979
  • In this study, enzymatic hydrolysis of the substrate of the waste wood of Cortinellus edodes was investigated using crude cellulase preparation of Trichoderma viride Pers. ex. Fr. SANK 16374. The crude cellulase was produced by the submerged culture process and produced in the culture fluid was salted out quantitatively by the use of ammonium sulfate. Reducing sugar was determined by the dinitrosalisylic acid (DNS) method. 1. The chemical composition of the waste wood was crude protein 2.26%, c. fat 2.57%, c. fibre 44.60%, c. ash 5.58% and lignin 13.62%. In amino acid composition, no cystine and methionine was showed, but trace amount of Vitamin A, $B_1$, and $B_2$, niacine and chloride were detected. (Table 1) 2. As heat treatment of the substrate was found to produce the highest reducing sugar yield being reacted for 48hr. with T.v cellulase, the substrate was heated to $190{\pm}5^{\circ}C$. for 45 min. either before or immediately after milling. 3. The substrate heated and ball milled at $190{\pm}5^{\circ}C$. for 45 min. the reducing sugar yield reached to 11.5%. 4. The substrate without any treatment was found to produce the highest reducing sugar yield being reacted 72hr. with T. v cellulase, the reducing sugar yield reached to 10.1%. 5. The rate of reducing sugar per each treated substrate was decreased by the order of the substrated, heated and then ball milled at $190{\pm}5^{\circ}C$. for 45 min. (11.5%)> without any treatment (10.1)> ball milled and heated at $190{\pm}5^{\circ}C$. for 45 min. (6.9%). 6. Saccharification of waste wood has been shown to be possible by heat treated and milling the substrate in contact with cellulase. And it is likely to be recommended that the waste wood may be valuable for raw materials of saccharification.

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Characteristics of Hot-Water Extracts from Salmon Frame as Basic Ingredients for Gomtang-like Products (열수추출 연어 Frame 엑스분의 곰탕 유사 제품 베이스로서의 특성)

  • Han, Byung-Wook;Kim, Hye-Suk;Jee, Seung-Joon;Lee, Jae-Hyoung;Kim, Hyung-Jun;Park, Shin-Ho;Ji, Seong-Gil;Heu, Min-Soo;Kim, Jin-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.10
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    • pp.1326-1333
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    • 2007
  • For preparing the basic ingredients of Gomtang-like products from the extracts of salmon frame, the extraction conditions of salmon frame were examined, and the characteristics of the extracts were compared with commercial Gomtang. Based on the crude protein, Ex-N and sensory attributes, the extractions were optimized by extracting pretreated-salmon frame in 12 times (v/w) of water for 12 hrs, before filtering with cheese cloth to yield 3 times the volume of the raw material. The concentrations of heavy metals in extracts from salmon frame were below the safety limits suggested by KFDA. The mai or amino acids were glutamic acid and aspartic acid as the free amino acids, and glycine, proline, and glutamic acid as the total amino acids. The calcium and phosphorus contents were 18.0 mg/100 mL and 33.1 mg/100 mL, respectively, and they accounted for 20% and 18% of the recommended daily allowance for mineral intake. The angiotensin I converting enzyme (ACE) inhibitory activity was improved by incubation with Flavourzyme for 4 hrs and its $IC_{50}$ was 2 mg/mL. The results above suggested that the enzymatic hydrolysates from extracts of salmon frame could be used as a basic ingredient for preparing Gomtang-like products.

A Study of the Vegetation in the Deogyusan National Park - Focused on the deciduous forest at Namdeogyu area - (덕유산국립공원의 식생에 관한 연구 -남덕유지역 낙엽활엽수림을 중심으로-)

  • Kim, Hyoun-Sook;Lee, Sang-Myong;Chung, Heung-Lak;Song, Ho-Kyung
    • Korean Journal of Environment and Ecology
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    • v.23 no.5
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    • pp.471-484
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    • 2009
  • This study was carried out to classify vegetation structure on the deciduous forest at Namdeogyu area in Deogyusan National Park from May 2007 to September 2008 using the gradient analyses and phytosociological method. The vegetation was classified into Quercus mongolica community(Typical subcommunity, Rhododendron sehlippenbaehii-Q. mongolica subcommunity, Carex lanceolata-Q. mongolica subcommunity), Q. Serrata community(Typical subcommunity, Q. variabilis -Q. serrata subcommunity, Carpinus laxiflora - Q. serrata subcommunity), C. laxiflora community, Fraxinus mandshurica community and Corn us controversa community. Ecological characteristics such as species composition, layer structure, vegetation ratio, and the distribution of individual trees by DBH(diameter at breast height) were significantly different among communities. The order of important value of the forest community with DBH 2cm above plants was Q. mongolica(67.8), F. mandshurica(44.8), Q. serrata(27.5), Acer pseudo-sieboldianum(17.0), C. controversa(l5.9), F. sieboldiana(l1.5), R. schlippenbachii(11.0), C. cordata(11.0), Styrax obassia(10.9), Magnolia sieboldii(10.4) and C. laxiflora(9.8). Distribution of DBH of Q. mongolica had a formality distribution, suggesting a continuous domination of these species over the other species for the time being. In contrast, F. mandshurica appeared limited to the valley of the sheet and a higher frequency of young individuals, suggesting a continuous domination of these species the development of a climax forest terrain. Q. serrata had a higher frequency of young individuals and middle individuals, suggesting a continuous domination of these species over the other species for the time being in contrast. This study examined the correlation between each community and the environment according to DCCA ordination. The Q. mongolica community predominated in the highest elevation habitats which had many C.E.C and T-N. F. mandshurica community predominated in the highest elevation habitats which had many Moisture and EX-Cation. The Q. serrata community and C. laxiflora community mainly occurred in the low elevation habitats which had few C.E.C and T-N. The C. controversa community appeared on the park in the middle habitats.

Radio location algorithm in microcellular wide-band CDMA environment (마이크로 셀룰라 Wide-band CDMA 환경에서의 위치 추정 알고리즘)

  • Chang, Jin-Weon;Han, Il;Sung, Dan-Keun;Shin, Bung-Chul;Hong, Een-Kee
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.23 no.8
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    • pp.2052-2063
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    • 1998
  • Various full-scale radio location systems have been developed since ground-based radio navigation systems appeared during World War II, and more recently global positioning systems (GPS) have been widely used as a representative location system. In addition, radio location systems based on cellular systems are intensively being studied as cellular services become more and more popular. However, these studies have been focused mainly on macrocellular systems of which based stations are mutually synchronized. There has been no study about systems of which based stations are asynchronous. In this paper, we proposed two radio location algorithms in microcellular CDMA systems of which base stations are asychronous. The one is to estimate the position of a personal station at the center of rectangular shaped area which approximates the realistic common area. The other, as a method based on road map, is to first find candidate positions, the centers of roads pseudo-range-distant from the base station which the personal station belongs to and then is to estimate the position by monitoring the pilot signal strengths of neighboring base stations. We compare these two algorithms with three wide-spread algorithms through computer simulations and investigate interference effect on measuring pseudo ranges. The proposed algorithms require no recursive calculations and yield smaller position error than the existing algorithms because of less affection of non-line-of-signt propagation in microcellular environments.

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Studies of Xanthium strumarium Extract Suppressing Th17-cell Differentiation and Anti-dermatitic Effect in BMAC-induced Atopy Dermatitis of NC/Nga Mice (창이자 추출물이 아토피 피부염 유발 생쥐의 비장 세포 Th17의 세포분화 억제에 따른 아토피 피부 상태에 미치는 영향)

  • Kim, Kum-Lan;Choe, Tae-Boo
    • KSBB Journal
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    • v.24 no.4
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    • pp.383-392
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    • 2009
  • Xanthii fructus which is well known as "Chang-ihjah" in Korea is the dried fruit of Xanthium strumarium L. (or Xanthium sibiricum PATR. Ex WIDD., Asteraceae. XS). Water extract of this fruit has been used for treatment of various inflammatory diseases such as tympanitis, allergic rhinitis, or ozena as alternative therapy material usually by oral administration in far Eastern countries including Korea. In this study, the effect of XS extract (XS-E) or XS-30% acetone fraction layer (XS-30% AFL) on the differentiation of $CD4^+$ T cells isolated from NC/Nga mouse and the production of IL-17 was investigated. The experimental results showed that $100\;{\mu}g$/mL of XS-E could decrease the production of IL-17 by $CD4^+$ Th17 cells by 2 fold and only $20\;{\mu}g$/mL of XS-30% AFL could inhibit 3.5 fold. The amount of IL-17A and IL-22 mRNA determined by real-time PCR was decreased remarkably when XS-E or XS-30% AFL was treated on $CD4^+$ Th17 cells(p<0.01, p<0.001). The amount of IL-17A protein determined by ELISA was also decreased remarkably(p<0.05, p<0.001). To study the effect of XS-E or XS-30% AFL on the proliferation of Th17 cells, $CD4^+$ T cells of a NC/Nga mouse was firstly differentiated by rIL-6/TGF-$\beta$ and then stimulated by rIL-23. The control group of Th17 cells were doubled every each day, while those of XS-E or XS-30% AFL treated group were shown to be delayed remarkably by these extracts. In conclusion, XS can inhibit the differentiation of Th17 cells of NC/Nga mouse and the production of IL-17 successfully, which may be a beneficial result for the treatment of atopic skin dermatitis.

Vegetation Structure and Site Characteristics of Rhododendron brachycarpum Population in South Korea (남한에서 만병초(Rhododendron brachycarpum D. Don ex G. Don) 개체군의 식생구조와 입지 특성)

  • Hwang, Yong;Han, Mu-Seok;Kim, Yong-Yul;Kim, Mu-Yeol
    • Korean Journal of Environment and Ecology
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    • v.28 no.6
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    • pp.751-761
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    • 2014
  • This study was carried out to analyze the vegetation properties, soil characteristics and ordination of Rhododendron brachycarpum population in South Korea. Rhododendron brachycarpum were mainly distributed along the Ulleungdo and Baekdudaegan of the Korean penninsula and it's population was located at an elevation of 872m to 1466m. The Rhododendron brachycarpum population was classified into Taxus cuspidata var. latifolia dominant population, Magnolia sieboldii dominant population, Thuja koraiensis dominant population and Rhododendron brachycarpum typical population. The composition of soil properties in the same areas are as follows: organic matter, total nitrogen, available phosphorous, exchangeable $K^+$, exchangeable $Ca^{2+}$, exchangeable $Mg^{2+}$ contained, and soil pH. The capacities of these chemical properties of the soil ranged from 10.45~15.28%, 0.37~0.61%, $0.21{\sim}0.35cmol^+/kg$, $0.39{\sim}2.54cmol^+/kg$, $0.17{\sim}0.50cmol^+/kg$, $18.28{\sim}22.81cmol^+/kg$ and 4.66~5.23 respectively. The results of the correlation between communities and soil conditions of vegetation of Rhododendron brachycarpum by DCCA ordination method are as follows: Taxus cuspidata var. latifolia dominant population was found in the very steep sloped area that has low percentage of total organic matter and nitrogen than other populations. Magnolia sieboldii dominant population and Thuja koraiensis dominant population was found in the steep sloped area that has high percentage of total organic matter and nitrogen than other populations. Thuja koraiensis dominant population was found in the gentle sloped area that has high percentage of altitudinal and rock exposure. Current status of Rhododendron brachycarpum is very vulnerable with a collection of herbs constantly threatening the species' survival. Thus, concrete conservation plans to protect natural habitats should be set up as soon as possible.

Development of Internet Information Push-Delivery System Design of Smoking Cessation for Health Promotion (지역주민의 건강증진을 위한 인터넷 금연 강화 프로그램 개발)

  • Kim, Young-Bok;Shin, Jun-Ho;Kim, Shin-Woel
    • Journal of agricultural medicine and community health
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    • v.29 no.2
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    • pp.287-301
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    • 2004
  • Objectives: The development of internet programs for smoking cessation was motivated to quit smoking in the large group of smokers. This personalized program consisted of tailored message to consider the smokers characteristics, and contain the informations on the outcomes of smoking cessation and the skills to be used in the quit attempts. The purpose of this study was to develop the internet management program and information push-delivery system for smoking cessation to encourage the personal intention to quit smoking. Methods: We conducted in 3 steps as developing push service to encourage intention of smoking cessation, analyzing problems of smoking cessation program through the pilot test and suggesting improvements by implication stages. Results: This program is delivered for 30 days. if the participants do not fail to quit smoking. The contents consisted of 13 stages which were divided on starting period. practical period, maintenance period and success period. And push service afforded the tailored message to participants using their e-mail. According to the evaluation of pilot test, the problems of internet information push-delivery service for smoking cessation were the over-tasks per visiting time, recording style of participants, difficulty of terms and sentences, lack of visual effects, absence of follow-up module and unsuitable link with main homepage. Improvements were divided on 3 stages by implication period. The first stage included the immediate improvements as improving link with homepage, modifying menu of smoking information and upload file of notice part. The second stage included the short term improvements as alleviating condition of withdrawal, coordinating start stage of retrial, modifying errors of information push-delivery service and addition of educational materials. The third stage included the long term improvements as development of follow-up module, cost-effectiveness evaluation, reducing contents quantity, introduction of checking style, compensation of graphics effect and review for SMS utilization. Conclusions: This program contribute to improving smoking cessation rate. Therefore this program should be tested in a community to evaluate the effectiveness. To promote the effectiveness, this program should be developed the contents and the strategies for various targets, and established the follow-up system for ex-smokers.

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A CYANOACETYLENE STUDY OF THE MOLECULAR DISK IN STAR FORMING REGIONS

  • Chung, H.S.;Kameya, Osamu;Morimoto, Masaki
    • Journal of The Korean Astronomical Society
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    • v.24 no.2
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    • pp.217-271
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    • 1991
  • We have observed dense core around young stellar objects, DR21, S140, Orion-KL, and L1551 using four millimeter-wave transitions of $HC_3N\;J$=4-3, J=5-4, J=10-9, and J=12-11. The spatial distribution of $HC_3N$ emission closely resembles the morphology of the previous CS observations that trace high density gas. These observations reveal the existence of $HC_3N$ dense cores around central IR source, elliptical in shape and almost perpendicular to the CO bipolar outflow axis. Small differences can be explained by that $HC_3N$ molecular line is more optically thin and is seen to be more detailed structure in the neighborhood of central IR sources. In S140 and Orion-KL, massive(${\sim}10\;M_{\odot}$), slowly rotating dense cores lie near at the central IR sources of bipolar outflows. The velocity channel maps of DR21 show that the bipolar outflow gas may have a correlation with the dense core of DR21. We analyzed intensities of the four lines to derive physical conditions in dense core from two methods, LTE and LVG. The column density of $HC_3N$, $N(HC_3N)$, between LTE and LVG calculations agree well with each other. The abundances of $HC_3N$ in each observing source have been estimated using the average values of $n(H_2)$ and $N(HC_3N)$ and assuming the size of dense core. The fractional $HC_3N$ abundances in massive dense cores of DR21, S140, and Orion-KL have a range of $(2-7){\times}10^{-10}$, while that of low mass dense core, L1551, has one order of magnitude greater value of $2{\times}10^{-9}$. This should be considered good agreement with the result by Morris et al.(1976). It may be considered that dense cores of DR21, S140, and Orion-KL may have almost same stage of chemical evolution, and their abundances have a small values relative to that of L1551. The column density $N(HC_3N)$ decreases with increasing distance from the densest part of the cloud, the central infrared source, and have the relation of $N(HC_3N){\varpropto}R^{\alpha}$, where a has a range of 0.65 to 0.89. The values of $n(H_2)$ are not varied with increasing distance from the dense core, and have almost same values. Therefore, it is considered that the dense cores in these regions probably consist of dense clumps in diffuse molecular gas medium, and $n(H_2)$ of each clump is ${\sim}10^5\;cm^{-3}$. Levels in the $T_{ex}$ increases with $n(H_2)$. It is considered that the $HC_3N$ dense cores are not completely thermalized. We examine the relationships between the luminosity of central infrared sources versus mass of the dense cores, and the luminosity of central infrared sources versus molecular hydrogen column density. Luminosities of the central IR sources show good correlation with mass and hydrogen column density of the dense core. Same has been found from CS observations. However, mass and size derived from $HC_3N$ observations are one order of magnitude smaller than those from CS. It can be interpreted that we see more central part of the cloud cores in $NC_3N$ lines than CS lines.

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Studies on Artificial Cultivation of Pleurotus eryngii (De Canolle ex Fries) Quel. (큰느타리(Pleurotus eryngii)의 인공재배에 관한 연구)

  • Lee, Dae-Jin;Kim, Kwang-Po;Lee, Byung-Eui
    • The Korean Journal of Mycology
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    • v.31 no.3
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    • pp.192-199
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    • 2003
  • This study was carried out to investigate the artificial cultivation of Pleurotus eryngii on the optimal medium vessel, periods of cultivation and the optimal method of pinheading for both yield and quality of fruiting body were also performed. The optimal composition of sawdust medium in polypropylene(PP) bottle was combination of sawdust(70%) and corncob(30%) but increased amount of corncob delayed the period of mycelial growth. The mycelial growth and the yield of fruiting body in the medium with beat pulp were worse than that without beat pulp. The optimal composition of nutrients for both yield and quality of fruit body tuned out to be a combination of rice bran(12%), wheat bran(12%) and cottonseed cake(6%). Additions of zeolite, shell lime and bean curd dregs were not effective in mycelial growth and yield of fruit body. When testing size of PP bottle for cultivation, the larger of bottle mouth is, the more pinheading number found, but the number of available fruit body is not significantly different. The culture in $1100\;ml-{\phi}75\;mm$ bottle is the best in the yield and quality of fruit body than those in $555\;ml-{\phi}50\;mm,\;850\;ml-{\phi}58\;mm,\;850\;ml-{\phi}65\;mm\;and\;1100\;ml-{\phi}65\;mm$ bottle. Using the PP bag for cultivation, a square shaped bag was better than a round shaped and black square shaped in mycelial growth and yield of fruit body. The most suitable period of incubation was 35 days after inoculation at $22{\pm}2^{\circ}C$. When the incubation periods was decreased less than 35 days, the pileus formation and yields were very bad but a pinheading condition looked similar, For an optimal pinheading, turning upside down was better than standing and covering.

In vivo Physiological Activity of Mentha viridis L. and Mentha piperita L. (박하의 in vivo 생리활성)

  • Lee, Seung-Eun;Han, Hee-Sun;Jang, In-Bok;Kim, Geum-Soog;Seong, Nak-Sul
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.6
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    • pp.261-267
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    • 2005
  • Alcohol metabolizing and antioxidant activity of Mentha species were investigated in rat liver. Fifty six Sprague Dawley rats were randomly divided into seven groups such as normal (ethanol excluded), negative control (40% ethanol (10 g/kg of body weight/day) fed), positive control (1 g Silymarin/kg of body weight/day with ethanol fed), two Mentha viridis extracts (0.2 g & 1 g M. viridis methanol ext./kg of body weight/day with ethanol fed) and two M piperita extracts (0.2 g & 1 g M. piperita methanol ext./kg of body weight/day with ethanol fed) groups. After 2 weeks, rats were sacrificed under ether. The activities of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), catalase (CAT), manganese superoxide dismutase (Mn-SOD), glutathione peroxidase (GAH-px) and the content ofthiobarbituric acid reactive substance (TBARS) in the rat livers and the activity of glutamate pyruvate transferase (GPT) in serum were evaluated. From the analyses, 1 g M. viridis and 0.2 g M. piperita administrated groups showed higher ADH and ALDH activity than the other groups. Groups fed with 0.2 g and 1 g M. viridis ext. and 0.2 g M. piperita ext. showed higher CAT activity than the other groups. All the Mentha extract fed groups exhibited more effective in recovering Mn-SOD, GSH-px and GPT acitivities to a similar degree of normal group. TBARS contents of two M. viridis ext. fed group and 0.2 g M. piperita ext. fed group were higher than those of the other groups. M. viridis extract fed groups showed more effective in CAT and Mn-SOD activities than M. piperita extract groups at p < 0.05. Finally, it is concluded that both Mentha species have alcohol metabolizing and antioxidant activity and M viridis is more effective than M. piperita.