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Antioxidant and NO-scavenging Activities of Acanthopanax senticosus var. subinermis Leaf Extracts Prepared Using Ethanol and Extrusion Processing

  • Lee, Sung-Hee;Oh, Hea-Young;Leem, Jae-Yoon;Yoon, Sun
    • Food Science and Biotechnology
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    • v.18 no.5
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    • pp.1124-1131
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    • 2009
  • Acanthopanax senticosus has been used in East Asia as a traditional medicine. The purpose of this study is the investigation of an extraction method for A. senticosus var subinermis (ASvS) leaves to improve their antioxidant and anti-inflammatory activities. Six extracts were prepared: extracted with water (W), ethanol (Eth), water or ethanol after ultra high pressure (WP, EthP), and water or ethanol after an extrusion process (WEx, EthEx). Diphenylpicrylhydrazyl (DPPH) radical scavenging activity and ferric reducing ability showed that all extracts had a significant antioxidant activity. In anti-inflammatory activities, Eth and EthEx significantly inhibited the release of lipopolysaccharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-${\alpha}$, and interleukin (IL)-$1{\beta}$ independent of cell viability in RAW 264.7 macrophage cells. Inducible NO synthase (iNOS) protein was significantly decreased by EthEx. These findings present that Eth and EthEx extracts of ASvS leaves have anti-inflammatory activities, and EthEx extract suppresses LPS-induced NO through the down-regulation of iNOS and pro-inflammatory cytokines.

Effect of Proprioceptive Neuromuscular Facilitation on Muscle Irradiation Patterns of the Affected Side of in Stroke Patients: A Preliminary Study (뇌졸중 환자에서 고유수용성신경근촉진법이 환측 근육의 방사형태에 미치는 영향: 사전연구)

  • Kim, Won-Ho
    • Physical Therapy Korea
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    • v.16 no.2
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    • pp.59-66
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    • 2009
  • This study investigated the irradiation pattern of muscles on the affected side in stroke patients after application of diagonal 2 extension (D2 ex) proprioceptive neuromuscular facilitation in the unaffected upper extremity. Seven stroke patients participated. Electromyographic activities were recorded in the iliopsoas, biceps brachii, and triceps brachii were recorded by surface electrodes at rest, D2 ex with knee extension, and D2 ex with knee 90 flexion, normalized by maximal voluntary isometric contraction (MVIC) values. The medial frequencies of biceps brachii in both sides were also measured to compare muscle type recruited. %MVIC of biceps brachii and triceps brachii were significantly higher in D2 ex than at rest (p<.05). There was no difference in median frequency of biceps brachii between the affected and unaffected side (p>.05). This suggests that proprioceptive neuromuscular facilitation using D2 ex may improve muscle activities of the paretic side and that the irritation pattern of the affected side may respond to maintain body equilibrium according to movement of the sound side.

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A HISTOPATHOLOGICAL STUDY ON THE PULP REACTION OF ADHESIVE RESINS AS PHOSPHORIC ESTER SYSTEM (인산(隣酸) 에스텔계 접착성(接着性) 레진의 치수반응(齒髓反應)에 관(關)한 병리조직학적(病理組織學的) 연구(硏究))

  • Kim, Chul-Ho
    • Restorative Dentistry and Endodontics
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    • v.10 no.1
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    • pp.7-16
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    • 1984
  • The purpose of this study was to investigate the pulpal responses to adhesive resins as phosphoric ester system: "Clearfil F II" (Composite filling material), "Panavia EX" (Composite cementing Material) and "Silar" (Microfilled Compsoite resin) comparing with Zinc-Oxide-Eugenol cement. Total 70 cavities of the permanent healthy teeth from 5 dogs were prepared and placed with experimental resins and Zinc-Oxide-Eugenol cement as control. The dogs were sarificed at 5 intervals of 3 days, one, two, four, six weeks. The specimens were routinely prepared and stained by Hematoxylin-Eosin. Followings were the results obtained through microscopic examination. 1. In cases of Clearfil F II and Panavia EX without etching and lining, pulp response in the early stage showed more severe vascular congestion and hemorrhage than that of Zinc-Oxide-Engenol cement. 2. The pulp response of totally etched cases was similar to that of unetched cases in the groups of Clearfil F II and Panavia EX. 3. The cases of Clearfil F II and Panavia EX with enamel etching showed no significant histologic change compared to that of total cavity etching. 4. The Silar case with total etching showed retarded tendency of histologic recovery compared to Clearfil F II and Panavia EX group. 5. Generally, pulp responses of experimental groups were not severe and the six week case showed the evidence of a histologic recovery.

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Development of a Modeling Methodology to Capture Collaborative Processes and Its Verification (협업프로세스 모델링 방법 개발 및 검증)

  • Lee, Sun-Hwa;Ryu, Kwang-Yeol
    • Journal of Korean Institute of Industrial Engineers
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    • v.36 no.3
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    • pp.176-185
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    • 2010
  • As long as the information systems are spread out, various efforts are being attempted to get the effective results by the system. As a result, a new management strategy has been appeared, referred to as "collaboration", and it is necessary to use a right methodology for modeling collaborative processes accordingly. Even though many modeling methodologies such as IDEF3, Petri-Nets, UML, and ARIS have been widely used for modeling processes, they are inadequate for clearly representing collaborative processes. Some researchers, therefore, have suggested new modeling methodologies for describing collaborative processes including CPM (collaborative process modeling). In this paper, we introduce an extended version of CPM method (i.e., exCPM) as a tool for modeling collaborative works after analyzing advantages and disadvantages of aforementioned methodologies. One of distinct characteristics of exCPM is that model verification is possible by transforming the exCPM models into Petri-Nets models. We also demonstrate transformation of an exCPM model in this paper with case studies for model verification.

ShEx Schema Generator for RDF Graphs Created by Direct Mapping

  • Choi, Ji-Woong
    • Journal of the Korea Society of Computer and Information
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    • v.23 no.10
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    • pp.33-43
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    • 2018
  • In this paper, we propose a method to automatically generate the description of an RDF graph structure. The description is expressed in Shape Expression Language (ShEx), which is developed by W3C and provides the syntax for describing the structure of RDF data. The RDF graphs to which this method can be applied are limited to those generated by the direct mapping, which is an algorithm for transforming relational data into RDF by W3C. A relational database consists of its schema including integrity constraints and its instance data. While the instance data can have been published in RDF by some standard methods such as the direct mapping, the translation of the schema has been missing so far. Unlike the users on relational databases, the ones on RDF datasets were forced to write repeated vague SPARQL queries over the datasets to acquire the exact results. This is because the schema for RDF data has not been provided to the users. The ShEx documents generated by our method can be referred as the schema on writing SPARQL queries. They also can validate data on RDF graph update operations with ShEx validators. In other words, they can work as the integrity constraints in relational databases.

A cytotaxonomic study of Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz.

  • Chung, Gyu-Young;Kim, Mi-Suk
    • Plant Resources
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    • v.3 no.3
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    • pp.179-183
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    • 2000
  • The present study was carried out to clarify the chromosome numbers and karyotype of Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz.. The somatic chromosome numbers of two species were same; basic chromosome number x=12, and somatic chromosome numbers 2n=24. The present result of A. japonica Koidz. ex Kitam. was same to previously reports and that of A. macrocephala Koidz. was reported first in this study. Size and shape of chromosome were some different from A. japonica Koidz. ex Kitam. and A. macrocephala Koidz.. The karyotype of A. japonica Koidz. ex Kitam. was described as follows; 2n : 24 : 8L + 14M +2S : 2 $A^{sm}$ +2 $B^{m}$ +2 $C^{m}$ +2 $D^{st}$ + 2 $E^{m}$ +2 $F^{m}$ +2 $G^{m}$ +2 $H^{sm}$ + 2 $I^{m}$ + 2 $J^{m}$ + 2 $K^{m}$ + 2 $L^{m}$ . And the karyotype of A. macrocephata Koidz. was described as follows; 2n : 24 : 10L +12M +25 : 2 $A^{m}$ +2 $B^{sm}$ +2 $C^{sm}$ +2 $D^{sm}$ +2 $E^{sm}$ +2 $E^{sm}$ +2 $G^{sm}$ +2 $H^{m}$ +2 $I^{m}$ 2 $J^{m}$ +2 $K^{m}$ +2 $L^{m}$ . .

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Effect of Harvesting Time and Storage Duration of Viscum album Seeds on in vitro and ex vitro Germination on the Branch of Prunus mume

  • Lee, Hyun Woo;Ghimeray, Amal Kumar;Lee, Bo-Duk;Sharma, Pankaja;Shim, Ie Sung;Park, Cheol Ho
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.256-262
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    • 2014
  • Viscum album var. coloratum (mistletoe) is considered as one of the endangered plant species in Korea. Our objective is to restore its population and multiplication of plant by ex situ method. In this research we explored the maximum germination (in vitro) of freshly harvested and stored seeds of mistletoe collected in different time intervals. Ex vitro germination after artificial inoculation on the branches of Prunus mume in different physiological conditions was also monitored. The research revealed that the lately harvested seeds (Feb. and March 2014) were superior over early harvested seeds (Nov. 2013 and Jan. 2014) of mistletoe due to the higher percentage of germination (above 93%). According to the data, it is also revealed that the survival and germination rate of mistletoe seeds decreased with the increase in storage duration. In ex vitro germination, the fluctuated temperature of a glass house in natural condition enhanced (four fold) the rate of germination on the branches of Prunus mume than the constant temperature condition in the glass house.

An Improvement Plan of Ex-post Settling up System on the Social Insurance of Construction Project - Focused on the National Pension and National Health Insurance - (건설공사 사회보험료 사후정산제도의 문제점 및 개선방안 - 국민연금 및 국민건강보험을 중심으로 -)

  • Oh, Chi-Don
    • Journal of the Architectural Institute of Korea Structure & Construction
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    • v.35 no.1
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    • pp.29-36
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    • 2019
  • In the past, social insurance premiums, such as national pension and national health insurance, is contained within the costs of construction projects, have been lowered due to price competition of companies participating in the bidding. The government is implementing the "Ex-post settlement system" to improve the problem. Therefore, the purpose of this study is to examine the concept of an Ex-post settlement system and background of its introduction and to identify problems in the management of the system. The problems of the Ex-post settlement system are: 1) avoiding the participation of construction workers, 2) the rigidity of the insurance premium settlement method in construction field, and 3) the cause of direct construction cost reduction. The ways to improve these problems are: 1) excluding social insurance premiums from construction project cost, 2) securing flexibility of the settlement method of insurance premiums in construction field, and 3) expanding the scope of persons eligible to participate in social insurance.

Inhibitory Effect of Fermented Spanish Extract on Inorganic phosphate-induced Vascular Calcification in ex vivo Aortic Rings (발효 시금치 추출물의 무기인산염에 의해 유도된 혈관 석회화 저해 효과)

  • Lee, Sang Hee;Hong, Sun Mi;Sung, Mi Jeong
    • Journal of the Korean Society of Food Culture
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    • v.37 no.3
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    • pp.248-255
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    • 2022
  • Spinach (Spinacia oleracea L.), a green leafy vegetable, is well known as a functional food due to its biological activities. Vascular calcification is associated with several disease conditions including atherosclerosis, diabetes, and chronic kidney disease (CKD), and is known to raise the risk of cardiovascular diseases related morbidity and mortality. However, there are no previous studies that have investigated the effects of fermented spinach exract (FSE) against aortic and its underlying mechanisms. Therefore, this study investigated the effects and action of possible mechanisms of FSE on inorganic phosphate (PI)-induced vascular calcification in ex vivo mouse aortic rings. PI increased vascular calcification through calcium deposition in ex vivo aortic rings. FSE inhibited calcium accumulation and osteogenic key marker, runt-related transcription factor 2 (Runx2), and bone Morphogenetic Protein 2 (BMP-2) protein expression in ex vivo aortic rings. And, FSE inhibited PI-induced extracellular signal-regulated kinase (ERK) and p38 phosphorylation in ex vivo aortic rings. These results show that FSE can prevent vascular calcification which may be a crucial way for the prevention and treatment of vascular disease association with vascular calcification.

Case Report: Intracapsular Carcinoma Ex Pleomorphic Adenoma of Parotid Gland (증례보고: 이하선에 발생한 다형선종 유래 피막내 암종 1예)

  • Lee, Seungyeol;Son, Hwangkyu;Park, Ho Sub;Song, Chang Myeon
    • Korean Journal of Head & Neck Oncology
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    • v.38 no.1
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    • pp.43-47
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    • 2022
  • Among a variety of malignant types for parotid gland tumors, intracapsular carcinoma ex pleomorphic adenoma which is classified as a non-invasive tumor has been reported rarely. We report a case of a 69-years old patient, who presented with a left parotid mass that was detected 30 years ago. Fine needle aspiration biopsy result of the mass was "suggestive of pleomorphic adenoma". Superficial partial parotidectomy was performed for the mass and the permanent pathologic finding was "intracapsular carcinoma ex pleomorphic adenoma" which was a salivary ductal carcinoma with well-preserved myoepithelial cells surrounding the malignant epithelial cell clusters. Surgical resection is the main treatment modality for the treatment of intracapsular carcinoma ex pleomorphic adenoma. Herein, we present the case with a review of literature.