• Fisheries and Aquatic Sciences
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• 제4권4호
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• pp.252-256
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• 2001

In this study, the bactericidal activity of each extract from Sawa-wasabi (Wasabia japonica) root, stem and leaf against the fish pathogenic bacteria were examined. The main component related to bactericidal activity in Sawa-wasabi was well known to AlT. The Sawa-wasabi roots showed the highest AIT amount with 1.18 mg/g. Stems was 0.41 and leaves was 0.38 mg/g. All of them showed bactericidal activity against 2 strains of Vibrio hollisae, V. anguillarum, and 2 strains of Edwardsiella tarda, but weak effect against Staphylococcus capitis. The Sawa­wasabi leaves showed the strongest bactericidal activity with minimal bactericidal concentrations (MBCs) of 156.3mg of dry weight/mL against 2 strains of V. hollisae, V. anguillarum and 2 strains of E. tarda. The roots and stems showed a little weak bactericidal activities with 312-1,250mg of dry weight/mL against them. These results suggest that certain components besides AIT in Sawa-wasabi are affective in killing fish pathogenic bacteria.

• Biotechnology and Bioprocess Engineering:BBE
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• 제4권1호
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• pp.12-16
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• 1999

Xenorhabdus nematophilus sp., an insect-pathogenic bacterium, was newly isolated from Korean entomopathogenic nematode of Steinernema carpocapsae, which can be used as a useful bioinsecticide. Primary and secondary form variants of Xenorhabdus nematophilus were observed when cultured in vitro. Primary form variants adsorbed bromothymol blue, while secondary form did not. However, many other characters of two variants were very similar. The variants were all rod-shaped and cell size was highly variable ranging from 0.5 by 2.0 ${\mu}$m to 1.0 by 5.0 ${\mu}$m. Both produced highly toxic substances and killed the insect larva within 20∼38 hr, indicating that insect pathogenicity of Xenorhabdus is not directly associated with its phase variation. In addition, cell-free culture supernatant of Xenorhabdus was sufficient to kill the insect larva by injecting it ito insect hemolymph; however, cell-harboring culture broth was more effective for killing the insect. The use of Xenorhabdus nematophilus may provide a potential alternative to Bacillus thuringiensis (Bt) toxins.

• Food Science and Biotechnology
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• 제17권2호
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• pp.219-227
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• 2008

Bacillus thuringiensis was isolated as a pathogen of the sotto disease of silkmoth larvae about a hundred years ago. Since then, this bacterium has attracted attentions of not only insect pathologists but also many other scientists who are interested in its strong and specific insecticidal activity. This has led to the recent worldwide development of B. thuringiensis-based microbial insecticides and insect-resistant transgenic plants, as well as a landmark discovery of par asp orin, a cancer cell-specific cytotoxin produced by B. thuringiensis. In this review, we describe examination of interaction between inclusion proteins of B. thuringiensis and brush border membrane of insects using a surface plasmon resonance-based biosensor, identification and characterization of parasporin-4, the latest parasporin produced by the B. thuringiensis A1470 strain, and an effective method for preparing the parasporin-4 from inclusion bodies expressed in the recombinant Escherichia coli cells.

• 한국섬유공학회:학술대회논문집
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• 한국섬유공학회 2003년도 봄 학술발표회 논문집
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• pp.48-51
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• 2003

Poly(vinyl alcohol) (PVA) obtained by the saponification of poly(vinyl ester) like poly(vinyl acetate) or poly(vinyl pivalate) is a linen. semicrystalline polymer, which have been widely used as fibers for clothes and industries, films, membranes, medicines for drug delivery system, and cancer cell-killing embolic materials[1-3]. PVA fibers and films have high tensile and compressive strengths, high tensile modulus, and good abrasion resistance due to its highest crystalline lattice modulus. (omitted)

• 호남수학학술지
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• 제33권4호
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• pp.557-573
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• 2011

Let G be a compact and connected semisimple Lie group, H a closed subgroup, g (resp. h) the Lie algebra of G (resp. H), B the Killing form of g, g the normal metric on the homogeneous space G/H which is induced by -B. Let D be an invarint connection with Weyl structure (D, g, ${\omega}$) in the tangent bundle over the normal homogeneous Riemannian manifold (G/H, g) which is projectively flat. Then, the affine connection D on (G/H, g) is a Yang-Mills connection if and only if D is the Levi-Civita connection on (G/H, g).

• IMMUNE NETWORK
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• 제11권5호
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• pp.245-252
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• 2011

Antimicrobial peptides/proteins are ancient and naturally-occurring antibiotics in innate immune responses in a variety of organisms. Additionally, these peptides have been recognized as important signaling molecules in regulation of both innate and adaptive immunity. During mycobacterial infection, antimicrobial peptides including cathelicidin, defensin, and hepcidin have antimicrobial activities against mycobacteria, making them promising candidates for future drug development. Additionally, antimicrobial peptides act as immunomodulators in infectious and inflammatory conditions. Multiple crucial functions of cathelicidins in antimycobacterial immune defense have been characterized not only in terms of direct killing of mycobacteria but also as innate immune regulators, i.e., in secretion of cytokines and chemokines, and mediating autophagy activation. Defensin families are also important during mycobacterial infection and contribute to antimycobacterial defense and inhibition of mycobacterial growth both in vitro and in vivo. Hepcidin, although its role in mycobacterial infection has not yet been characterized, exerts antimycobacterial effects in activated macrophages. The present review focuses on recent efforts to elucidate the roles of host defense peptides in innate immunity to mycobacteria.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.155.1-155.1
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• 2015

Nowadays, Plasma has been used in biological, medical such as wound healing, plant grow, killing cancer. When plasma generated, UV light and ROS(Reactive oxygen species), RNS(Reactive nitrogen species) can generated and those things effect to biological material. So we made simple plasma device using needle type of electrode and generated plasma. We used three kinds of gas and measured applied voltage and current. Also we observed optical emission spectrum. Using deuterium ramp, we can observed absorption spectrum and calculated radical density by lambert-beer's law. It is around ~1016cm3. And we can see the time-resolved absorption spectrum from monochromator, PMT(photo multiply tube), IV-converter, oscilloscope.

• 한국환경성돌연변이발암원학회지
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• 제26권1호
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• pp.25-29
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• 2006

흥합 (Mytilus coruscus) 추출물 RM 60을 사용하여 E. coli D31을 대상으로 항균활성 펩타이드를 분리 정제하였다. 정제한 펩타이드는 MALDI-TOF Mass spectrometry를 사용하여 분자량을 측정한 결과, 1464.92 Da로 나타났으며 Edman 분해법을 이용한 peptide sequencer를 사용하여 일차구조를 분석 중에 있다.

• IMMUNE NETWORK
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• 제14권4호
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• pp.201-206
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• 2014

IL-33 is a member of the IL-1 cytokine family and plays a role in the host defense against bacteria, viruses, and fungi. In this study, we investigated the function of IL-33 and its receptor in in vitro macrophage responses to Candida albicans. Our results demonstrate that pre-sensitization of isolated peritoneal macrophages with IL-33 enhanced their pro-inflammatory cytokine production and phagocytic activity in response to C. albicans. These macrophage activities were entirely dependent on the ST2-MyD88 signaling pathway. In addition, pre-sensitization with IL-33 also increased ROS production and the subsequent killing ability of macrophages following C. albicans challenge. These results indicate that IL-33 may increase anti-fungal activity against Candida through macrophage-mediated resistance mechanisms.

• 한국섬유공학회:학술대회논문집
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• 한국섬유공학회 2002년도 봄 학술발표회 논문집
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• pp.187-190
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• 2002

Poly(vinyl alcohol) (PVA) is a representative hydrophilic and water-soluble polymer and widely employed in various applications such as fibers for clothes and industries, films, membranes, medicines for drug delivery system, and cancer cell-killing embolic materials. Moreover, PVA fibers, gels, and films are potentially high-performance materials because they have high tensile and impact strengths, high tensile modulus, high abrasion resistance, excellent alkali resistance, and oxygen barrier property which are superior to those of any known polymers[1,2]. (omitted)