• 대한한의학회지
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• 제31권6호
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• pp.8-15
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• 2010

Objectives: We investigated to develop and validate HPLC-PDA methods for simultaneous determination of eight constituents in Galgeun-tang (GGT). Methods: Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 230 nm, 254 nm, and 280 nm, were used for quantification of the eight marker components of GGT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results: Calibration curves were acquired with $r^2$ > 0.9999, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each component was in the range of 87.33-101.38%, with an RSD less than 7.0%. The contents of the eight components in GGT were 1.98-12.17 mg/g. Conclusions: The established method will be applied for the quantification of marker components in GGT.

• 대한한의학방제학회지
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• 제18권1호
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• pp.95-103
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• 2010

Objectives : To develop and validate HPLC-PDA methods for simultaneous determination of seven constituents in Samsoeum(SSE). Methods : Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array(PDA) detection at 254 and 280 nm, were used for quantification of the seven marker components of SSE. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results : Calibration curves were acquired with $r^2$>0.9997, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each compound was in the range of 100.07-112.65%, with an RSD less than 4.0%. The contents of seven compounds in SSE were 1.24-10.53 mg/g. Conclusions : The established method will be helpful to improve quality control of SSE.

• Journal of the Korean Wood Science and Technology
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• 제48권3호
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• pp.364-375
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• 2020

In this study, ultrasound-assisted extraction was performed to extract ascorbic acid from rugosa rose (Rosa rugosa Thunb.) fruit. The optimal conditions were investigated by response surface methodology, using two variable including reaction time (16-44 min) and temperature (16-44℃). The ascorbic acid extraction was sensitive to the reaction time rather than the reaction temperature, and the optimal conditions for ascorbic acid extraction were 25℃ and 30 min. Ascorbic acid and gallic acid in the rugosa rose fruit extract were completely separated by HPLC, with a resolution factor of over 1.5 between the two. The correlation coefficient of the ascorbic acid was 0.999 in a linearity test for 50-150 ㎍/mL concentration of extract. The limit of detection and limit of quantification values were 0.16 ㎍/mL and 29.89 ㎍/mL, respectively. The relative standard deviations (RSD) for repeatability and reproducibility were determined, and each RSD showed good precision at less than 5% (N=6).

• Smart Structures and Systems
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• 제26권1호
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• pp.23-33
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• 2020

Majority of the damage in engineering structures is nonlinear. Damage sensitive features (DSFs) extracted by traditional methods from linear time series models cannot effectively handle nonlinearity induced by structural damage. A new DSF is proposed based on vector space cosine similarity (VSCS), which combines K-means cluster analysis and Bayesian discrimination to detect nonlinear structural damage. A reference autoregressive moving average (ARMA) model is built based on measured acceleration data. This study first considers an existing DSF, residual standard deviation (RSD). The DSF is further advanced using the VSCS, and then the advanced VSCS is classified using K-means cluster analysis and Bayes discriminant analysis, respectively. The performance of the proposed approach is then verified using experimental data from a three-story shear building structure, and compared with the results of existing RSD. It is demonstrated that combining the linear ARMA model and the advanced VSCS, with cluster analysis and Bayes discriminant analysis, respectively, is an effective approach for detection of nonlinear damage. This approach improves the reliability and accuracy of the nonlinear damage detection using the linear model and significantly reduces the computational cost. The results indicate that the proposed approach is potential to be a promising damage detection technique.

• 대기
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• 제21권3호
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• pp.311-317
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• 2011

Aerosol optical properties from the radiation measurements by SKYNET PREDE skyradiometers, POM-01 and POM-02 were compared during the inter-calibration campaign at Seoul in February 2009. The monochromatic solar flux at the top of the atmosphere ($F_0$) gave a relative standard deviation (RSD) of 9-10% for both instruments. This comparatively high value of RSD was probably because $F_0$ was determined at short time intervals, in the morning and afternoon, using the measurements made in the polluted environment of Seoul. Although POM-02 was more effective in tracking the solar radiation, aerosol optical depths (AODs) from the two instruments were very similar after the cloud screening procedure. The squared correlation coefficients ($R^2$) of single scattering albedo (SSA) and real and imaginary refractive indices between the two instruments was around 0.5 but increased to 0.7-0.8 when only using AOD greater than 0.4. Nevertheless, mean values of the Angstrom exponent, SSA, and the imaginary refractive index of POM-02 were higher than those of POM-01.

• Fisheries and Aquatic Sciences
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• 제19권1호
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• pp.3.1-3.6
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• 2016

Phlorotannins are reported to have diverse biological properties. However, no analytical methods for the standardization of phlorotannin preparations have been reported. Herein, we developed and validated an analytical method for the determination of dieckol in phlorotannin extracts (PRT) using high-performance liquid chromatography (HPLC). The optimum HPLC conditions consisted of a Supelco Discovery C18 column stationary phase, a mobile phase (A: 15 % HPLC grade methanol in deionized water, B: methanol), UV detection at 230 nm, and a flow rate of 0.7 mL/min. The optimized chromatographic conditions were validated and exhibited good specificity and linearity ($R^2$ > 0.9994-1.0000). The recoveries were in the range of 100.9-102.3 %. The method had good intermediate (%RSD 1.2) and intra-day (%RSD 0.4-1.7) assay precisions. This HPLC method had good accuracy and quality in the determination of dieckol in PRT.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.407-407
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• 2000

For acquisition of 3-Dimensional information in real space, stereo vision system is suitable. In the stereo system, 3D real world position is derived from translation of coordinates between cameras and world. Thus, to use stereo vision, it is needed to construct a precise system which provides kinematically precise translation between camera and world coordinate, in spite of intricacy and hardness. So much cost and time should be spent to build the system. In this paper, facilely to solve previous problem, a method which can easily obtain 3D informations using reference objects and RSD(Relative Stereo Disparity) is proposed. Instead of direct computation of position with translation of coordinates, only relative stereo disparity in stereo pair of image is used to find the reference depth of objects, and real 3D position is computed with initial condition of reference objects. In computation, subpixel resolution is involved to find the display for accuracy. To find the RSD, corresponding points are calculated in subpixel resolution. So the result in experiemnt will be shown that subpixel resolution is more accurate than 1 pixel resolution.

• 한국식품위생안전성학회지
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• 제14권1호
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• pp.76-83
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• 1999

Solid phase microextraction (SPME) was used for the determination of 6 standard solvents (methanol, isopropanol, methyl ethyl ketone, ethyl acetate, cyclohexane, toluene) in food packaging materials. SPME method is a solvent-free sample preparation technique in which a fused silica fiber coated with polymeric organic liquid is introduced into the headspace above the sample. SPME method using fiber coated polydimethylisiloxane (PDMS) was compared with static headspace (SHS) method used as a reference. It was found that the optimal adsorption condition using PDMS-SPME method was 2$0^{\circ}C$ for 15 minutes for the standard solvents. Detection limits, linearity, reproducibility and recovery of both SHS and PDMS-SPME methods have been determined using 6 standard solvents. Both methods were characterized by high reproducibility and good linearity. Using SHS methods, the mean recovery of the 6 standard solvents was ranged from 75.5% to 105.8% with a mean relative standard deviation (RSD) of 0.3% to 4.8%. With PDMS-SPME method, the mean recovery of the 6 standard solvents was ranged from 86.7% to 108.3% with a mean RSD of 0.4% to 2.5%. The detection limits of both methods were the same for toluene, cyclohexane and methyl ethyl ketone; those of PDMS-SPME method were higher than those of SHS method for methanol, isopropanol and ethyl acetate. PDMS-SPME fiber shoed excellent adsorption for non-polar solvents such as toluene, while it showed relatively low adsorption for polar solvents such as methanol.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권11호
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• pp.5436-5458
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• 2017

Automatic Video Surveillance System (AVSS) has become important to computer vision researchers as crime has increased in the twenty-first century. As a new branch of AVSS, baggage detection has a wide area of security applications. Some of them are, detecting baggage in baggage restricted super shop, detecting unclaimed baggage in public space etc. However, in this paper, a detection & classification framework of baggage is proposed. Initially, background subtraction is performed instead of sliding window approach to speed up the system and HSI model is used to deal with different illumination conditions. Then, a model is introduced to overcome shadow effect. Then, occlusion of objects is detected using proposed mirroring algorithm to track individual objects. Extraction of rotational signal descriptor (SP-RSD-HOG) with support plane from Region of Interest (ROI) add rotation invariance nature in HOG. Finally, dynamic human body parameter setting approach enables the system to detect & classify single or multiple pieces of carried baggage even if some portions of human are absent. In baggage detection, a strong classifier is generated by boosting similarity measure based multi layer Support Vector Machine (SVM)s into HOG based SVM. This boosting technique has been used to deal with various texture patterns of baggage. Experimental results have discovered the system satisfactorily accurate and faster comparative to other alternatives.

• Archives of Pharmacal Research
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• 제27권5호
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• pp.539-543
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• 2004

An efficient analytical method was devised for the accurate L-muscone assay in aqueous samples. It involves solid-phase extraction of L-muscone in adsorption mode using XAD-4 as the sorbent and dichloromethane modified with 10% (v/v) methanol as the eluting solvent. The gas chromatographic analysis of the eluate residue dissolved in toluene on a DB-5MS capillary column provided complete resolution of L-muscone from the co-extracted interferences. The overall method showed excellent linearity ($r^2{\geq}$ 0.9994) in the range of 0.1 to 2.0 $\mu\textrm{g}$/mL with good intra- and inter-day precisions (% RSD = 2.5～7.3) and with high extraction recovery rates ($\geq$ 98.1 %). When the present method was applied to a L-muscone herbal drink product, the within-batch RE (%) in the labeled concentration (1.5 $\mu\textrm{g}$/mL) for the three randomly chosen bottles were -2.4, -1.3 and -3.3 with high precision (% RSD $\leq$ 3.1). The present method is considered to be suitable for quality control evaluation on liquid drinks and other complex formulations fortified with L-muscone.