• 대한본초학회지
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• 제36권2호
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• pp.31-42
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• 2021

Objectives : This study was undertaken to investigate the hepatoprotective effect of Spatholobi Caulis water extract (SC) to thioacetamide (TAA)-induced acute liver injury (ALI) in rats. Methods : The rats were injected intraperitoneally with TAA (200 mg/kg body weight) and orally administered SC (100 or 200 mg/kg b.w.) daily for 3 days. Liver biomarkers were assessed by serum glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and ammonia levels. Malondialdehyde (MDA) was measured both serum and liver tissue. In addition, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, anti-oxidant, and inflammation-related proteins were investigated by western blot analysis. Histological examination further confirmed though hematoxylin and eosin stain. Results : The SC treatment reduced liver function markers like GOT and GPT and also remarkably decreased ammonia level. Moreover, the elevated MDA level in TAA-induced group was significantly reduced by SC treatment. NADPH oxidase expression associated with oxidative stress including NOX2, NOX4, and p47phox markedly inhibited by SC administration. SC treatment exerted anti-oxidant effect through the increase of anti-oxidant enzyme including superoxide dismutase (SOD), Catalase, and heme oxygenase-1 (HO-1). The protein expressions of inflammatory cytokines such as tumor necrosis factor-�� (TNF-��), IL-6, and IL-1�� induced by nuclear factor-kappa B (NF-��B) activation were modulated through blocking the phosphorylation of inhibitor of nuclear factor ��B�� (I��B)��. SC treatment also improved histological alterations. Conclusion : These findings suggested that SC administration may be a potential candidate for the prevention or treatment of ALI.

• 한국식품과학회지
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• 제53권5호
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• pp.544-552
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• 2021

본 연구에서 in vitro를 통해 백작약 열수 추출물의 total polyphenol, total flavonoid 함량, DPPH 및 ABTS radical 소거능 측정으로 높은 항산화능을 확인했으며, 백작약 열수 추출물 투여로 인한 AST, ALT, ammonia 및 MPO 수치의 호전을 확인하였다. 또한 간 조직을 이용한 western blot 실시 후 모든 인자에서 유의적인 차이를 확인하였다. 이 결과를 토대로, 백작약 열수 추출물이 TAA로 인한 산화적 스트레스를 Nrf2/Keap1 경로의 활성화를 통해 SIRT1/AMPK/NF-kBp65를 조절하였으며, 섬유화 관련 단백질의 발현을 억제함으로써 간섬유증에 대한 백작약 열수 추출물의 긍정적인 효과가 있음을 제시하였다. 따라서 선행 연구를 통해 TAA로 유발한 급성 간손상 동물 모델에서 간보호 효과를 나타낸 백작약 열수 추출물이 TAA로 유발한 간섬유증 동물 모델에서 산화적 스트레스 억제와 간 기능 개선을 나타냈으며 향후 후보 소재로서 가능성이 있다고 판단된다.