• Journal of Bio-Environment Control
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• v.23 no.4
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• pp.288-292
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• 2014

This study was performed to investigate the possibility of water cutting by vegetative propagation method from July to August of Clematis. To find out proper cutting length and rooting growth regulator for water cuttings production, cutting lengths of C. 'Honora' made 1 node and 2 nodes length cutting, which were soaked in NAA $0.1mg{\cdot}L^{-1}$. Rooting rate of cuttings treated by NAA $0.1mg{\cdot}L^{-1}$ was 100% irrespective of root lengths, but that of 1 node and 2 nodes untreated by NAA $0.1mg{\cdot}L^{-1}$ were very low about 30% and 19% respectively. Number of roots and roots length were also highest in NAA $0.1mg{\cdot}L^{-1}$, effects of NAA $0.1mg{\cdot}L^{-1}$ treatment were greater at 1 node cutting. Based on the results, rooting rate for I node cutting of C. 'Pairu' according to NAA (0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$) concentrations was investigated. Days to rooting were 70-74 days (about 10 weeks), which was not significantly different among the NAA concentrations. Rooting rate increased by NAA concentration. it was about 90% usually and was improved with more than NAA $0.5mg{\cdot}L^{-1}$.

• Progress in Medical Physics
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• v.11 no.1
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• pp.73-83
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• 2000

In this study, we investigate the effects of poor shimming on quantitative measurement of ratios of metabolite levels by proton magnetic resonance spectroscopy ($^1$H MRS). Coefficient of variation (COV) of metabolite ratios for point resolved spectroscopy (PRESS) and stimulated-echo acquisition mode (STEAM) spectra was evaluated from a phantom containing in vivo levels of metabolites using a conventional whole body 1.5T MR system and conventional acquisition and analysis protocol. A statistical P-value was also calculated from a linear regression for relationship of metabolite ratios. N-acetylaspartate (NAA)/ creatine (Cr) and NAA/ choline (Cho) had low COV values for the long and short TE spectra (29.1 and 27.5%; 23.8 and 12.6 %), whereas Cho/Cr and Cr/Cho had high COV values (50.0 and 68.6 %; 27.5 and 29.3 %). A linear relationship between NAA/Cr and Cho/Cr, and between NAA/Cho and Cr/Cho revealed the statistical significance in the long and short TE spectra, respectively (P < 0.0001 and P < 0.0001; P = 0.015 and P = 0.005). There was no significant relationship between Cho/NAA and Cr/NAA in the measurement (P = 0.159; P = 0.910). The present study suggested that NAA/Cr and NAA/Cho could be useful for data with poor shimming in $^1$H MR spectroscopy. In conclusion, statistical significance of metabolite ratios indicated that the Cr and Cho levels could be interpreted as a significant alteration factor in the long and short TE spectra, and then should be used with care to provide precise metabolite quantification.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.21-27
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• 1997

This experiments were conducted to determine the effect of thidiazuron on forming tuberlets and plant regeneration of Pinellia ternata T. by tissue culture. The addition of $5\;{\mu}M$ TDZ to the medium had better regeneration than that of any other treatments of NAA and TDZ. At the combination treatments of NAA and TDZ, as the level of thidiazuron increased, the rate of shoot regeneration was incresed while the increment of NAA concentration inhibited the rate of shoot regeneration. The supplement of $5\;{\mu}M$ thidiazuron produced the best number of micro-tubers per explant and the number of micro-tuber formed was 25 in MS medium and 29 in MG medium on 30 day culture, respectively. Microtuber formation was the best on MG medium with 1.0 mg/l NAA and $5\;{\mu}M$ thidiazuron. MG medium was superior to MS and B5 medium for the growth of tuberlets. Half strength of MS medium with NAA 2 mg/l was the most effective for root formation. Rooting ability on nursery soil of plantlets produced in in vjtro was good as a 80% after 3 weeks.

• Korean Journal of Medicinal Crop Science
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• v.1 no.1
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• pp.43-48
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• 1993

Present experiments were carried out to examine the effect of plant growthregulators for callus induction and plantlet regeneration through leaf tissue culture of Scutellaria baicalensis GEORGI. The results indicated that Callus was induced well on MS medium supplemented with 0.5mg/L NAA or 0.5mg/L NAA Plus 0.5mg/L zeatin. MS medium supplemented with 1.0mg/L BAP plus 0. 5mg /L NAA or 1. 0mg /L zeatin Plus 0.5mg /L NAA and 1.0mg /L NAA were the most effective for plant regeneration. Thin layer chromatogram of baicalin component (Rf 0.39) was observed from callus cultured on MS medium containing 0.5mg /L NAA plus 0.5mg /L zeatin.

• Current Research on Agriculture and Life Sciences
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• v.18
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• pp.1-7
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• 2000

This study was carried out to determine the effects of plant growth regulators on organogenesis from Cymbidium kanran and Cymbidium hybrida. Optimal rhizome formation from Cymbidium kanran was obtained on MS medium with 10 ppm kinetin+2 ppm NAA. and optimal protocorm formation from Cymbidium hybrida was obtained on MS medium with 10 ppm kinetin+0.05 ppm NAA. However, in this study the optimal media for the callus induction from both explants was not identified. Optimal shoot induction from rhizome of Cymbidium kanran was obtained on MS medium with 10 ppm BA+2 ppm NAA and 5 ppm BA+2 ppm NAA. Optimal shoot induction from protocorm of Cymbidium hybrida was obtained on MS medium with 10 ppm kinetin+2 ppm NAA.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.540-545
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• 2014

The Polygonatum odoratum cv. Gungangbeaksea, bred in Gyeongsangnam-Do Agricutural Research & Extension Service, was cultured in vitro for micropropagate rapidly through the culture of rhizome explants ($5{\times}5mm$). The $7{\times}7mm$ explants of adventitious multi-bud clusters (AMC), obtained through the culture of rhizome explants (MS + 3.0 mg/L BA) were cultured on MS media with BA and TDZ. The shoot multiplication was favorable on the MS medium containing 3.0 mg/L TDZ with 2.8 in shoot number. But the formation of AMC was low in all media tested. The explants of AMC were cultured on MS media containing 1.0~5.0 mg/L TDZ and NAA to multiplicate AMC more. The formation of AMC was a little more stimulated on combined MS media of TDZ and NAA, than that with TDZ alone. The multiplication of shoots and AMC was favorable on MS media with 3.0 mg/L TDZ and 5.0 mg/L NAA, and 5.0 mg/L TDZ and 3.0 mg/L NAA. As the concentration of MS salts increased, the formation of AMC was decreased. But the formation of AMC was more stimulated, as the concentration of sucrose increased to 7%. Therefore, the multiplication of shoots and AMC was suitable on media containing 3.0~5.0 mg/L TDZ and NAA, and 7% of sucrose. The explants of AMC were rooted on media with 3.0 mg/L IBA, or 2.0 mg/L NAA with more than 80% in rooting ratio. The plantlets were treated at $5^{\circ}C$ for 8 weeks, and cultured ex vitro for 8 weeks. The survival ratio of plantlets were 100% in vermiculite, and the mixed soil with perlite 1 volumn and vermiculite 1 volumn.

• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.362-367
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• 2008

In order to investigate the effect of plant growth regulators on effective in vitro micropropagation, apical meristems of Pulsatilla cernua var. koreana were cultured on Murashige and Skoog's (MS) medium with 2,4-D, NAA, TDZ and BA. Media containing 2,4-D and kinetin, 2,4-D and TDZ, NAA and TDZ were not effective on callus induction. However, embryogenic or organogenic callus was obtained on media containing NAA and BA. Especially, on MS medium with 0.5mg/L NAA and 1.0mg/L BA was optimal for a high frequency (62%) of shoot or shoot bud obtained from callus. Callus proliferation, shoot multiplication and elongation were significantly increased by adding 10% coconut water on MS media with 0.5mg/L NAA and 1.0mg/L BA. Repeated subculturing of in vitro grown shoots resulted in propagation rate of 12.9 shoots per explant every 30 days. Root formation from the adventitious shoots was not easily achieved. However, roots were only produced through callus on MS medium with 2.0mg/L NAA alone or 0.5mg/L NAA and 1.0mg/L BA. These roots were used materials for callus and shoot production repetitively.

• Journal of Plant Biotechnology
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• v.2 no.3
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• pp.151-155
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• 2000

Shoot proliferation was obtained from shoot tips and nodal explants of Vitex negundo L. on MS medium supplemented with either BAP or KIN (0.1-2.0 mg/L) alone or in combination with NAA (0.1 mg/L). The concentrations of cytokinins combined with NAA produced multiple shoots from shoot tips and nodal explants. The highest mean percentage (84.3$\pm$8.0) of shoot multiplication's were observed on nodal explants in the presence of BAP (1.5 mg/L) and NAA (0.1 mg/L) followed by shoot tips (65.0$\pm$5.0). The regenerated shootlets were rooted on MS basal medium IAA, IBA, NAA (0.1-1.5 mg/L). The maximum number of roots (51.0$\pm$2.6) was achieved on the medium containing IBA (1.0 mg/L) followed by other auxins (NAA, IAA). The regenerated plants were successfully transferred to a mixture of vermiculate and soil. About 95% of the plantlets survived when transferred to the field.

• Plant Resources
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• v.6 no.3
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• pp.233-241
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• 2003

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

• Investigative Magnetic Resonance Imaging
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• v.8 no.2
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• pp.86-93
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• 2004

Purpose: We evaluated $^1H$ MR spectroscopy improves the preoperative diagnosis of diffuse infiltrative type of primary central nervous system lymphomas. Materials and methods: After review of conventional MR images and medical records, we retrospectively reviewed proton MR spectra in seven patients with primary central nervous system lymphoma diagnosed by means of biopsy. Relative ratio of choline (Cho), N-acetylaspartate (NAA), and lipid-lactate (Lip-Lac) to creatine (Cr) were measured for quantitative analysis. Results: The average ratio of Cho/Cr was $1.80{\pm}0.95$, NAA/Cr was $1.34{\pm}0.41$, and Lip-Lac/Cr was $1.12{\pm}0.16$. All cases of lymphomas showed increased Lip-Lac peak. Two case of mass forming lymphoma showed decreased NAA/Cr significantly, whereas five cases of lymphoma without mass formation showed preserved NAA/Cr. Conclusion : We thought the presence of Lip-Lac peak without significant reduction of NAA on the MR spectroscopy was helpful for diagnosis of diffuse infiltrative type of central nervous system lymphoma.