• Food Science of Animal Resources
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• v.44 no.2
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• pp.408-429
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• 2024

The color, lipid oxidation, heme iron (HI) and non-heme iron (NHI) contents, metmyoglobin content and Soret band of myoglobin of ground pork subjected to supercritical CO₂ treatment under different conditions, or to heat treatment (40℃, 2 h) and subsequent storage at 4℃ were evaluated during 9-day period. Supercritical CO₂ treatment significantly increased CIE L^* and CIE b^* values of ground pork during subsequent storage, while the HI content was slightly affected. In general, CIE a^* value and metmyoglobin content were decreased. Supercritical CO₂ treatment for 2 h could increase the thiobarbituric acid-reactive substances (TBARS) value, while treatment for 1 h or less had no effect. The NHI content could be increased only after treatment at above 40℃ or 17.2 MPa for 2 h. The Soret band of myoglobin was shifted to longer wavelength. Increasing treatment temperature from 35℃ to 45℃ could increase CIE L^*, CIE a^*, CIE b^* and TBARS values, HI and NHI contents of the ground pork, while decreasing metmyoglobin content. As the treatment pressure increased from 13.8 MPa to 20.7 MPa, CIE b^* and TBARS values were decreased, while the NHI and metmyoglobin contents were increased. However, the other parameters were unchanged. Extending exposure time from 0.5 h to 2 h could increase CIE L^*, CIE b^* and TBARS values, HI contents, while decreasing CIE a^* value and metmyoglobin content. Correlation analysis showed that the TBARS value was significantly and negatively correlated with the HI content or metmyoglobin content in samples treated at 40℃ or above for 2 h.

• Korean Journal of Food Science and Technology
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• v.36 no.5
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• pp.693-700
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• 2004

Viscometry was applied to identify irradiated corn starch (CS), sweet potato starch (SS), and potato starch (PS) from non-irradiated controls using Brookfield DV-III programmable rheometer. Effects of starch suspension concentration (7.0-9.5%) and spindle speed (25-125 rpm) were investigated. Established optimal viscosity-measuring conditions showed the highest correlation coefficient between irradiation dose (0-6.0 kGy) and corresponding viscosities for CS, SS, and PS. Threshold values for identifying irradiated starches were suggested. Viscosities of all samples significantly decreased with increasing irradiation dose (p<0.05) and spindle speed, while increased as suspension concentration increased. Optimal conditions for suspension concentration and spindle speed were 7.5% (6.7%, d.b.) and 125rpm in CS, 8.5% (7.3%, d.b.) and 125 rpm in SS, and 9.0% (7.3%, d.b.) and 100rpm in PS, respectively. Under these measuring conditions, threshold values for discriminating unknown samples were 0.313, 0.345, and 0.811 for CS, SS, and PS in 1.5 kGy-irradiated samples, compared with 0.521, 0.798, and 1.693 in non-irradiated samples, respectively, enabling identification of irradiated from non-irradiated starches.

• Journal of the Korean Society of Radiology
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• v.84 no.5
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• pp.1110-1122
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• 2023

Purpose This study aimed to assess the variability of transrectal shear wave elastography (SWE) using a designed phantom. Materials and Methods In a phantom, the SWE values were examined by two radiologists using agarose and emulsion silicone of different sizes (1, 2, and 3 cm) and shapes (round, cubic) at three depths (1, 2, and 3 cm), two region of interest (ROI) and locations (central, peripheral) using two ultrasound machines (A, B from different vendors). Variability was evaluated using the coefficient of variation (CV). Results The CVs decreased with increasing phantom size. Significant changes in SWE values included; agarose phantom at 3 cm depth (p < 0.001; machine A), 1 cm depth (p = 0.01; machine B), emulsion silicone at 2 cm depth (p = 0.047, p = 0.020; both machines). The CVs increased with increasing depth. Significant changes in SWE values included; 1 cm agarose (p = 0.037, p = 0.021; both machines) and 2 cm agarose phantom (p = 0.047; machine A). Significant differences in SWE values were observed between the shapes for emulsion silicone phantom (p = 0.032; machines A) and between ROI locations on machine B (p ≤ 0.001). The SWE values differed significantly between the two machines (p < 0.05). The intra-/inter-operator agreements were excellent (intraclass correlation coefficient > 0.9). Conclusion The phantom size, depth, and different machines affected the variability of transrectal SWE.

• Journal of Food Hygiene and Safety
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• v.38 no.5
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• pp.356-360
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• 2023

We analyzed the heat resistance of non-pathogenic Bacillus atrophaeus, Bacillus subtilis, and Geobacillus stearothermophilus spores which exhibit strong heat resistance and evaluated the possibility of using them to determine direct sterilization when manufacturing retort foods. The D₁₂₁-values of B. subtilis, G. stearothermophilus, and B. atrophaeus spores were 2.9±0.1 min, 4.3±0.1 min, and 3.7±0.1 min, respectively. The Z-values of B. subtilis, G. stearothermophilus, and B. atrophaeus spores were 43.0±1.4℃, 25.0±1.6℃, and 35.8±1.4℃, respectively. The D₁₂₁-values of B. subtilis, G. stearothermophilus, and B. atrophaeus spores were all higher than that of Clostridium botulinum spores used to confirm retort food sterilization. Considering these results, B. subtilis, G. stearothermophilus, and B. atrophaeus spores can be used instead of the pathogenic spore-forming bacteria C. botulinum when sterilizing retort food. In addition, sterilization can be confirmed in 2 to 3 days, a shorter time than the 13 days required for existing bacterial growth experiments based on the Korean food code.

• Journal of dental hygiene science
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• v.22 no.3
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• pp.156-163
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• 2022

Background: This study attempted to apply resin infiltrant (RI) as a method to maintain the effect of tooth bleaching treatment and compared it with fluoride varnish (FV) or artificial saliva to evaluate the effect. Methods: Sixty healthy lozenge specimens were classified into five groups. Group 1 was the negative control group, and discoloration was induced after artificial saliva treatment of the tooth specimen (G1_S+C). Group 2 was a positive control group, in which pigmentation was induced after bleaching treatment and artificial saliva treatment (G2 _B+S+C). Coloration was induced in group 3 (experimental group 1) after bleaching treatment and artificial saliva treatment, followed by application of fluorine varnish (G3_B+FV+S+C). Coloration was induced in Group 4 (experimental group 2) after applying RI after bleaching treatment and artificial saliva treatment (G4_B+RI+S+C). Pigmentation was induced in group 5 (experimental group 3) after bleaching treatment and artificial saliva treatment, followed by acid treatment (etching) and treatment with RI (G5_B+E+RI+S+C). Coffee and wine were used to induce discoloration. The lightness value (L^*) of the CIE L^*a^*b^* color system was obtained by image analysis. Kruskal-Wallis H analysis was performed for the mean difference in L^* values by group. Results: When coloration was induced with coffee, there was no significant difference in L^* value between artificial saliva (G2 _B+S+C), FV (G3_B+FV+S+C), and RI (G4_B+RI+S+C, G5_B+E+RI+S+C) groups. There was no significant difference in L^* values between the artificial saliva (G2 _B+S+C), FV (G3_B+FV+S+C), and RI (G4_B+RI+S+C, G5_B+E+RI+S+C) groups, even in the case of wine induced coloration. Conclusion: It was confirmed that artificial saliva or RI treatment had similar effects to the FV previously used to maintain the effect of tooth bleaching treatment.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.3
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• pp.303-311
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• 1996

The microflora changes of 10 water-controled treatments combined with livestock manures(pig, chicken) and bulking agents(sawdust, paper sludge) were investigated. The B/F values of the P-1 and C-1(65%, $H_2O$) treatments were 3571 and 5400 respectively, but those of the P-4 and C-4(50%, $H_2O$) treatments showed very low values, 667 and 334, respectively. The B/F values tended to increase with higher water content of the treatments. In the composting processes, the successions of microflora, adapting the compost environments, took place via fluctuating temperature. In the high temperature period, the numbers of mesophilic bacteria and fungi decreased, but that of the spore forming bacteria increased. However, the number of mesophilic bacteria inereased during the cold period. The B/F values of compost ranged 25-300, which indicates a decrease in the quantity of bacteria. The time required for the temperature of compost to reach $60^{\circ}C$ showed different patterns. There was no pathogenic microorganism in the treatments which reached a high temperature in a short period of time, but, in the treatments which reached a high temperature over a Long period of time, the pathgenic microorganism was not still alive.

• Natural Product Sciences
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• v.12 no.4
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• pp.205-209
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• 2006

The structures of flavonoids, 2(S)-5,7-dihydroxy-8,2'-dimethoxyflavanone (1), wogonin (2), 2(S)-5,7, 2'-trihydroxy-8-methoxyflavanone (3), and 2(S)-5,2',5'-trihydroxy-7,8-dimethoxyflavanone (4), isolated from Scutellaria indica were revised on the basis of 2D NMR spectroscopy, including to gCOSY, gHSQC, and gHMBC. Compounds 1-4 were tested in vitro protein tyrosine phosphatase 1B (PTP1B) inhibitory activity. Compounds 2 and 4 exhibited weak PTP1B inhibitory activity with $IC_{50}$ values of 208 and $337{\mu}M$, respectively.

• The Journal of Korean Academy of Prosthodontics
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• v.32 no.1
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• pp.9-22
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• 1994

Porcelain and resin are widely used in esthetic dentistry. In the field of esthetic dentistry, the color and color stability of prosthetic materials are very important in esthetics. In this study, porcelain and resin with the same color were used to veneer on the 3 different metal alloys. Color was evaluated by the CIE $L^*a^*b^*$ system. The purpose of this study was evaluated the cole, difference and cole, stability of the specimens, according to the veneered materials and types of metal alloys. Specimens were divided into 6 groups as follows ; Group 1; Porcelain on the precious metal alloy Group 2; Porcelain on the semiprecious metal alloy Group 3; Porcelain on the nonprecious metal alloy Group 4; Resin on the precious metal alloy Group 5; Resin on the semiprecious metal alloy Group 6; Resin on the nonprecious metal alloy The results obtained were as follows; 1. In porcelain specimens, $L^*$ values showed no significant difference and $a^*$ value of group 2 was lower than that of groups 1, 3 and $b^*$ value of group 2 was higher than that of groups 1, 3. The ${\Delta}E^*ab$ values between group 1-2, group 2-3 were higher than 1.5. 2. In resin specimens. $L^*$ value of group 6 was lower than that of groups 4, 5, $a^*$ value of group 4 was higher than that of group 6, $b^*$ value of group 4 was higher than that of groups 5, 6. The ${\Delta}E^*ab$ values between group 4-5, group 5-6 were higher than 1.5, and between group 4-6 was higher than 3.0. 3. Comparing with veneered materials, $L^*$ flues of porcelain were higher than that of resin. 3. Comparing with veneered materials, $L^*$ values of porcelain were higher than that of resin. In semiprecious metal alloy, $a^*$ values of porcelain were lower than that of resin. In semiprecious and nonprecious metal alloy, $b^*$ values of porcelain were higher than that of resin. The ${\Delta}E^*ab values were higher than 6.0. 4. The color stability of resin specimen was relatively inferior to porcelain specimen.

• Bulletin of the Korean Chemical Society
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• v.22 no.12
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• pp.1361-1365
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• 2001

Recombinant immunotoxins are hybrid cytotoxic proteins designed to selectively kill cancer cells. A divalent immunotoxins, [B3(FabH1)-PE38]2, was constructed by recombining Fab domain of B3 antibody as a cell-targeting domain and Pseudo monas exotoxin A (PE) as a cytotoxic domain. Monoclonal antibody, B3, is the murine antibody (IgG1k) directed against Lewis Y-related carbohydrate antigens, which are abundant on the surface of many carcinomas. Fab fragment of this antibody was used in this study with the modified hinge sequence where last two cysteines out of three were mutated to serine. PE is a 66 kDa bacterial toxin that kills eukaryotic cells by inhibiting protein synthesis with ADP ribosylation of ribosomal elongation factor 2 (EF2). Fc region of B3 antibody was substituted with the truncated form of PE (38 kDa, PE38) on DNA level. [B3(FabH1)-PE38]2 was formed by disulfide bond between cysteines in the modified hinge region of B3(FabH1)-PE38. Each polypeptide for recombinant immunotoxins was overexpressed in Escherichia coli and collected as inclusion bodies. Each inclusion body was solubilized and refolded, and cytotoxic effects were measured. Divalent immunotoxins, [B3(FabH1)-PE38]2, had ID50 values of about 10 ng/mL on A431 cell lines and about 4 ng/mL on CRL1739 cell lines. Control immunotoxins, B3(scFv)-PE40, had ID50 values of about 28 ng/mL on A431 cell lines and about 41 ng/mL on CRL1739 cell lines. Divalent immunotoxins, [B3(FabH1)-PE38]2, had higher cytotoxic effects than B3(scFv)-PE40 control immunotoxins.

• The Journal of Korean Academy of Prosthodontics
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• v.33 no.4
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• pp.634-644
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• 1995

The purpose of this study is to evaluate the color differences in the In-Ceram according to coping thickness and various abutment core materials ; amalgam, precious alloy, composite resin, non-precious alloy. After the porcelain was built up on the In-Ceram coping at the thicknes of 0.3mm and 0.5mm then it was cemented with glass ionomer cement to the post & core materials. The following results were obtained. 1. There were significant differences in the $L^*$ values $a^*$ and $b^*$ values in the cementation of different cores, to the In-Ceram(P<0.01). $L^*$ values were not significantly different between the composite resin to the In-Ceram and the $a^*\;b^*$ values were not significantly different between the amalgam and the non-precious alloy. 2. All of cementations of In-Ceram to the core materisal had color difference(${\Delta}E^*ab$) compared to the In-Ceram. In the 0.3mm thickness of the In-Ceram copping non-precious alloy indicated the greatest value, while the composite resin core showed the lowest value with a thickness of 0.5mm In-Ceram copping. 3. By controlloing the In-Ceram coping thickness $L^*$ value was significatly different(P<0.01), but not in $a^*$ and $b^*$ values. 4. In an amalgam, precious & non-prcious alloys there was a 1,74 to 3.06 range color difference in the controlled thickness of In-Ceram coping at the thickness of 0.3mm and 0.5mm. The above results suggest that the requirement of the sufficient thickness of In-Ceram coping and the suitable core material in order to get an estheti restoration by In-Ceram and also to intercept the original core color.