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REAL-TIME MEASUREMENT OF DENTINAL TUBULAR FLUID FLOW DURING AND AFTER AMALGAM AND COMPOSITE RESTORATIONS (아말감과 복합레진의 수복 과정과 수복 후 발생하는 상아세관액 흐름의 실시간 측정)

  • Kim, Sun-Young;Cho, Byeong-Hoon;Baek, Seung-Ho;Lim, Bum-Sun;Lee, In-Bog
    • Restorative Dentistry and Endodontics
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    • v.34 no.6
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    • pp.467-476
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    • 2009
  • The aim of this study was to measure the dentinal tubular fluid flow (DFF) during and after amalgam and composite restorations. A newly designed fluid flow measurement instrument was made. A third molar cut at 3 mm apical from the CEJ was connected to the flow measuring device under a hydrostatic pressure of 15 $cmH_2O$. Class I cavity was prepared and restored with either amalgam (Copalite varnish and Bestaloy) or composite (Z-250 with ScotchBond MultiPurpose: MP, Single Bond 2: SB, Clearfil SE Bond: CE and Easy Bond: EB as bonding systems). The DFF was measured from the intact tooth state through restoration procedures to 30 minutes after restoration, and re-measured at 3 and 7days after restoration. Inward fluid flow (IF) during cavity preparation was followed by outward flow (OF) after preparation, In amalgam restoration, the OF changed to IF during amalgam filling and slight OF followed after finishing. In composite restoration, application CE and EB showed a continuous OF and air-dry increased rapidly the OF until light-curing, whereas in MP and SB, rinse and dry caused IF and OF, respectively. Application of hydrophobic bonding resin in MP and CE caused a decrease in flow rate or even slight IF. Light-curing of adhesive and composite showed an abrupt IF. There was no statistically significant difference in the reduction of DFF among the materials at 30 min. 3 and 7 days after restoration (p > 0.05).

Characterization of Fusant from Protoplast Fusion between Saccharomyces cerevisiae D-71 and Zygosaccharomyces rouxii SR-S (Saccharomyces cerevisiae D-71과 Zygosaccharomyces rouxii SR-S의 세포융합으로 육성한 융합주의 특성)

  • 이종수;김찬조
    • Microbiology and Biotechnology Letters
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    • v.16 no.4
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    • pp.297-302
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    • 1988
  • The protoplasts of Saccharomyces cerevisiae D-71, a thermophilic strain and Zygosaccharomyces rouxii SR-S, an osmotolerant strain were fused, and a fusant (FS-RN 1) was selected, then was characterized for its genetic stability, DNA content, cell capacity, growth rate, tolerance to salts and chemicals, $\beta$-fructofuranosidase level and ethanol fermenting activity. After 6 months of preservation, 5.8% of the fusant clones were segregated to parental types. The DNA content and cell capacity of the fusant were greater than those of the parental strains. Lag period of growth for the fusant was longer than those for the parents. The fusant colonies showed pink-color reaction to triphenyltetrazolium chloride(TTC) test. The fusant appeared to have resistances to NaCl at moderate levels between both parental strains, and resistances to KCI, sodium propionate and cycloheximide similar to either one of the parents. $\beta$-Fructofuranosidase activity of the fusant was 24.2$\times$10$^{-3}$/IU/g(dry wt) for 3 days culture. Ethanol yields ofter 4 days of fermentation by the fusant at 3$0^{\circ}C$ were : 6.0%(v/v) from 40% of glucose and 8.8%(v/v) from 20% of sucrose.

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A study on the camera working of 3D animation based on applied media aesthetic approach - Based on the Herbert Gettl's theory - (영상미학적 접근의 3D 애니메이션 카메라 워킹 연구 - 허버트 제틀의 이론을 중심으로 -)

  • Joo, Kwang-Myung;Oh, Byung-Keun
    • Archives of design research
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    • v.18 no.3 s.61
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    • pp.209-218
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    • 2005
  • Consciously or not, producers have to make many aesthetic choices in creative process of video production. If there are general acceptable aesthetic principles to make right choice it would be guideline of aesthetic decision to somewhat reduce mistakes and errors in the process. This paper proposes a theoretical approach on establishing the media aesthetic principle of 3D animation camera working, which is the most suitable for animation production context. We describe the Herbert Zettl's applied media aesthetics related directly to the camera, which is about the two-Dimensional field focusing on aspect radio and forces within the screen, three-dimensional field focusing on depth, volume, and four-dimensional field focusing on time and motion. In order to have theoretical approach we made an analysis on comparing a camera working of movie with 3D computer animation's one, and reconstructed these basic principles to be suited for the 3D animation production. When applied media aesthetics of the traditional camera working are applied to the 3D animation production, it could be an efficient guideline for it. Futhermore, if we develop the research for the relationship with various visual languages with the basis of these principles, the theory of creative picture composition method for the 3D animation production will be logically and systematically established.

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Effects of dietary supplementation of a lipid-coated zinc oxide product on the fecal consistency, growth, and morphology of the intestinal mucosa of weanling pigs

  • Byun, Young-Jin;Lee, Chul Young;Kim, Myeong Hyeon;Jung, Dae Yun;Han, Jeong Hee;Jang, Insurk;Song, Young Min;Park, Byung-Chul
    • Journal of Animal Science and Technology
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    • v.60 no.1
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    • pp.29.1-29.6
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    • 2018
  • Background: Dietary supplementation of zinc oxide (ZnO) to 2000 to 4000 mg/kg is known to be effective for the prevention and treatment of post-weaning diarrhea in the pig. Such a 'pharmacological' supplementation, however, can potentially result in environmental pollution of the heavy metal, because dietary ZnO is mostly excreted unabsorbed. Two experiments (Exp.) were performed in the present study to determine the effects of a lipid-coated ZnO supplement Shield Zn (SZ) compared with those of ZnO. Methods: In Exp. 1, a total of 240 21-day-old weanling pigs were fed a diet supplemented with 100 mg Zn/kg as ZnO (ZnO-100), ZnO-2500, SZ-100, or SZ-200 in 24 pens for 14 days on a farm with its post-weaning pigs exhibiting a low incidence of diarrhea. Exp. 2 was performed using 192 24-day-old piglets as in Exp. 1 on a different farm, which exhibited a high incidence of diarrhea. Results: In Exp. 1, fecal consistency (diarrhea) score (FCS) was less for the ZnO-2500 and SZ-200 groups than for the SZ-100 group (P < 0.05), with no difference between the SZ-100 and ZnO-100 groups. Both average daily gain (ADG) and gain:feed ratio were less for the SZ-200 group than for the ZnO-2500 group, with no difference between the ZnO-100 group and SZ-100 or SZ-200 group. The villus height (VH), crypt depth (CD), and VH:CD ratio of the intestinal mucosa were not influenced by the treatment. In Exp. 2, FCS was lowest for the ZnO-2500 group, with no difference among the other groups. However, neither the ADG nor gain:feed ratio was influenced by the treatment. Conclusion: Results suggest that physiological SZ supplementation has less beneficial effects than pharmacological ZnO for the alleviation of diarrhea irrespective of its severity and for promoting growth without influencing their integrity of the intestinal mucosal structures with little advantage over physiological ZnO in weanling pigs with a small pen size.

Kinetic Analysis of the Salto Side-Ward Tucked on the Balance Beam (평균대 옆공중돌기 동작의 운동역학적 분석)

  • Yeo, Hong-Chal;Chang, Jae-Kwan
    • Korean Journal of Applied Biomechanics
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    • v.18 no.3
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    • pp.61-69
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    • 2008
  • The purpose of this study is to examine the success or failure on the balance beam in element group requirements posture which is bending salto side-ward tucked through kinetic analysis. The national team players were participated. The goal was to present training methods to coaches and athletes so as to provide scientifically useful information. The results from this study were summarized as below. When the performance was successful, the features of the body's center of gravity during the side somersault motion showed to spread from the center of the balance beam and the center of the gravity moved to the direction of the body's rotation. In the spring sections - event2 and 3, when the performance was successful, up/down fluctuation became more wider and increased air time. It supported the result that the projecting variable was higher than in failure trial. In addition, the right side hip joint angles and speed, and angular velocity as jumping up for a leap were larger than in failure trial. Those variables showed the optimal conditions for a leap. By increasing the speed of the upper limb from the shoulder and the speed of the shoulder joint angular velocity, the momentum was increased. Especially the right side shoulder joint angular velocity increased dramatically because the right leg was held. As to the side somersault motion, the angular momentum of successful trial with respect to x-axis was bigger than failed trial. It indicated that the increasing angular momentum with respect to x-axis was an important factor in flying motion. Besides, as to side somersault, the appropriate proportion of angular momentum with respect to y-axis and z-axis was a key to successful trails.

Structural Analysis of Natural Indigo Colorants Extracted from Polygonum tinctorium (천연인디고 색소의 구조분석)

  • Chung, In-Mo;Lee, Kwang-Gill;Sung, Gyou-Byung;Kim, Hyun-Bok;Nam, Sung-Hee;Hong, In-Pyo
    • Journal of Sericultural and Entomological Science
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    • v.49 no.1
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    • pp.8-13
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    • 2007
  • Natural indigo colorants were prepared by extraction of Polygonum tinctorium which was harvested just in the blooming season(in the late of July). The components were analyzed by TLC and HPLC, and its structures were analyzed by FT-IR, EI-mass. The results obtained are summarized as follows; The natural indigo powder was dissolved in DMSO and developed in eluent, $CHCI_3/CH_3CN$(8.5:1.5 v/v) by means of TLC for its quality analysis. It was segregated into indirubin as a red colour and indigo as a blue colour. In case of HPLC analysis,. FT-IR spectrum of indirubin showed a peak for NH residue between 3200 and $3300cm^{-1}$. $^1H-NMR$ spectrum for indigo displayed AA'BB' spin system caused by indole structure between 6.5 and 7.7ppm of H4, 5, 6 and 7, and -NH proton for indirubin showed an singlet between 10.88 and 11.0ppm. EI-mass spectrum of indigo and indirubin both disclosed their molecular size as 262 and it implies that these two substances are isomer.

The Variation of Population of Pinus koraiensis Sieb. et Zucc. in Mt. Jiri (지리산(智異山) 잣나무집단(集團)의 변이(變異)에 관(關)한 연구(硏究))

  • Lee, Kang Young
    • Journal of Korean Society of Forest Science
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    • v.34 no.1
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    • pp.1-14
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    • 1977
  • These studies were carried out to know the variation of morphological tree forms, needles, cotyledons, seed characters and top height growth of seedlings of Pinus koraiensis S. et Z. by populations in Mt. Jiri. The results are summarized as follows: 1. It was observed that the characteristics of length, needles of serration density of needles, percentage of full seeds length, of seeds, thickness, of seeds length of cotyledons, width of cotyledon, numbers of the stomata line in cotyledons and the top height growth of seedlings were significantly different in individual trees and populations. 2. The characteristics of branch angle specific gravity of wood and resin canal numbers of cotyledons were significantly different in populations. 3. It was that the characteristics of numbers of stomata line, width, of seeds weight, of seeds serration density of cotyledons, numbers of cotyledons were significantly different in individuals. 4. The resin canals of needles showed three principle resin canals in individual trees and population, and the coefficients of variation were not shown in individual trees and populations. Stem forms showed to be a straight form with all populations. 5. The characteristics of cotyledons had generally higher tendency than the characteristics of needles in comparison with coefficience of variation.

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Quantitative Analysis of Electrophysiological Characteristics of CIDP and CMT Type 1: Sensory Nerve Research (CIDP와 CMT 1형의 전기생리학적 특성에 대한 정량 분석: 감각신경연구)

  • Kang, Ji-Hyuk
    • Korean Journal of Clinical Laboratory Science
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    • v.53 no.2
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    • pp.151-157
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    • 2021
  • Charcot-Marie-Tooth disease (CMT) is a slowly progressive hereditary degenerative disease and chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is an acquired immune-mediated disorder characterized by weakness and sensory deficits. The purpose of this study was to analyze and compare the electrophysiological characteristics observed in sensory nerve conduction studies (SNCS) of both diseases. A retrospective study of 65 patients with a diagnosis of CIDP (N=35) and CMT type I (N=30) was performed. This study analyzed No potentials ratio, distal compound nerve action potential (dCNAP) of various nerve types, and a correlation coefficient analysis of the sensory nerve conduction velocity (SNCV). As a result, I found that CMT 1 was more severe systemic demyelinating and axonal polyneuropathy better than CIDP (P<0.05). In a quantitative analysis of dCNAP and SNCV, especially sural nerve was the most severe nerve injury observed in both diseases. In correlation and scatter plot analysis, CMT 1 showed relatively high correlations compared to CIDP based on the correlation coefficient analysis (Fisher's Z test) of SNCV. The results of this study suggested that CMT 1 showed the slowness in SNCV, one of the characteristics of demyelinating polyneuropathy, and this slowing had a uniform pattern. In conclusion, electrophysiological characteristic of SNCS may be useful in the diagnosis and research between patients with CMT 1 and CIDP.

The Mediating Effect of Resilience on the Relationship between Nurse Practice Environment and Organizational Commitment in Clinical Nurses (임상간호사의 간호근무환경과 조직몰입의 관계에서 회복탄력성의 매개효과)

  • Kim, Sanguook;Seo, Minjeong
    • Journal of Digital Convergence
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    • v.18 no.12
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    • pp.435-444
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    • 2020
  • This study is a descriptive research to grasp the effects of nurse practice environment on organizational commitment and to confirm the mediating effect of resilience. The subjects of this study were 150 nurses who agreed to participate in this study among clinical nurses working in university hospital and General hospital located in City J and City J and the data were collected through survey. The collected data were analyzed by SPSS 25.0 program. Mean scores were organizational commitment 3.38, nurse practice environment 2.62, and resilience index 3.45. There were significant differences on the organizational commitment index for : age, marriage, clinical experience, job satisfaction and a positive correlation between nurse practice environment and organizational commitment, between nurse practice environment and resilience index and between organizational commitment and resilience index. As a result of research, resilience partially mediated the relationship between nurse practice environment and organizational commitment. The findings suggest that in order to improve the organizational commitment of nurse, converged organizational commitment arbitration program strategies are needed to improve the nurse practice environment and resilience of nurses.

Impacts of stream water quality and fish histopathology by effluents of wastewater treatment plant (하수종말처리장 배출수에 의한 하천 수질 특성 및 어류의 조직병리학적 영향)

  • Kim, Hye-Jin;An, Kwang-Guk
    • Korean Journal of Environmental Biology
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    • v.38 no.4
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    • pp.678-690
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    • 2020
  • In this study, the histological changes of Zacco platypus exposed to discharge from a Wastewater Treatment Plant (WTP), which is a point source, for a long time (2 to 3 years) were compared to the same species at a reference site (Ref.). Overall, tissues displayed various lesions in samples obtained at the point where discharge water from the point source was mixed. In the skin tissue, mucus cells from the epithelial layer expanded to the multilayered epithelium, indicating the immune system was activated. Epithelial cell detachment and proliferation were most prominent in the gills, which may have adversely affected circulation and respiration. Our data suggest immune system collapse was due to stimulation by aquatic substances. Both the fish phase analysis and the water quality analysis demonstrated depreciated conditions at the point source as compared to the reference stream, supporting the histological health evaluation results. These data together suggest a histological approach can also be used to assess water quality, and to an even higher degree when combined with other existing methods. Given the presented evaluation, improvement in the water quality of water discharged from WTP's is required.