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Antimicrobial susceptibility and pathogenic genes of Staphylococcus aureus isolated from the oral cavity of patients with periodontitis

  • Kim, Ga-Yeon;Lee, Chong Heon
    • Journal of Periodontal and Implant Science
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    • v.45 no.6
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    • pp.223-228
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    • 2015
  • Purpose: The goal of this study was to characterize the patterns of antimicrobial resistance and virulence genes in samples of Staphylococcus aureus (S. aureus) isolated from periodontitis patients. Methods: From July 2015 to August 2015, oral saliva was collected from a total of 112 patients diagnosed with periodontitis, including 80 outpatients in dental hospitals and 32 patients in dental clinics located in Seoul and Cheonan. The samples were subjected to a susceptibility test to evaluate the prevalence of antimicrobial resistance, and the pathogenic factors and antimicrobial resistance factors in the DNA of S. aureus were analyzed using polymerase chain reaction. Results: A susceptibility test against 15 antimicrobial agents showed that 88% of cultures were resistant to ampicillin, 88% to penicillin, and 2% to oxacillin. Resistance to at least two drugs was observed in 90% of cultures, and the most common pattern of multidrug resistance was to ampicillin and penicillin. Enterotoxins were detected in 65.9% of samples. The cell hemolysin gene hld was detected in 100% of cultures and hla was detected in 97.6% of samples. All strains resistant to penicillin and ampicillin had the blaZ gene. The aph(3')IIIa gene, which encodes an aminoglycoside modifying enzyme, was detected in 46.3% of samples. Conclusions: In the treatment of oral S. aureus infections, it is important to identify the pathogenic genes and the extent of antimicrobial resistance. Furthermore, it is necessary to study patterns of antimicrobial resistance and cross-infection in the context of periodontological specialties in which antimicrobials are frequently used, such as maxillofacial surgery, where the frequency of antimicrobial use for minor procedures such as implant placement is increasing.

A NEW TYPE 1 AGN POPULATION AND ITS IMPLICATION ON THE AGN UNIFIED MODEL

  • Yi, Sukyoung K.;Oh, Kyuseok;Schawinski, Kevin;Koss, Michael;Trakhtenbrot, Benny
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.43.1-43.1
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    • 2015
  • We have discovered an unexplored population of galaxies featuring weak broad-line regions (BLRs) at z < 0.2 from detailed analysis of galaxy spectra in the Sloan Digital Sky Survey Data Release 7. These objects predominantly show a stellar continuum but also a broad $H{\alpha}$ emission line, indicating the presence of a low-luminosity active galactic nucleus (AGN) oriented so that we are viewing the central engine directly without significant obscuration. These accreting black holes have previously eluded detection due to their weak nature. The new BLR AGNs we found increased the number of known type 1 AGNs by 49%. Some of these new BLR AGNs were detected at the Chandra X-ray Observatory, and their X-ray properties confirm that they are indeed type 1 AGN. Based on our new and more complete catalogue of type 1 AGNs, we derived the type 1 fraction of AGNs as a function of [OIII] ${\lambda}5007$ emission luminosity and explored the possible dilution effect on the obscured AGN due to star-formation. The new type 1 AGN fraction shows much more complex behavior with respect to black hole mass and bolometric luminosity than suggested by the existing receding torus model. The type 1 AGN fraction is sensitive to both of these factors, and there seems to be a sweet spot (ridge) in the diagram of black hole mass and bolometric luminosity. Furthermore, we present a hint that the Eddington ratio plays a role in determining the opening angles. This work is submitted to ApJS.

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HERITABILITIES AND GENETIC CORRELATIONS OF EGG QUALITY TRAITS IN TAIWANS'S LOCAL CHICKEN

  • Chen, C.F.;Lee, Y.P.;Lee, Z.H.;Huang, S.Y.;Huang, H.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.3
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    • pp.433-440
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    • 1993
  • Means and standard errors of 285 Taiwan's local chicken and 429 Single Comb White Leghorn pullets at 35 wk of age were: egg weight (g) $48.3{\pm}0.3$, $54.6{\pm}0.3$, shell index $73.39{\pm}0.26$, $73.20{\pm}0.18$, shell color $15.23{\pm}0.40$, $0.88{\pm}0.27$, shell whiteness $72.61{\pm}0.57$, $90.00{\pm}0.35$, shell strength ($kg/cm^2$) $3.77{\pm}0.07$, $3.35{\pm}0.05$, shell thickness (mm) $0.38{\pm}0.003$, $0.38{\pm}0.002$, Haugh units $85.26{\pm}0.50$, $91.81{\pm}0.38$ and yolk percent (%) $30.17{\pm}0.18$, $27.32{\pm}0.16$ respectively. Theestimated heritabiliities of Taiwan's local chicken based on sire and dam components of variance were as follows: egg weight 0.20, shell index 0.1, shell color 0.87, shell whiteness 0.79, shell strength 0.37, shell thickness 0.14, Haugh units 0.24 and yolk percent 0.16. Genetic correlations based on sire and dam components of variance and covariance were also estimated. Generically, the shell index was positively correlated with egg weight, shell strength and yolk percent, and egg weight was negatively correlated with shell thickness, Haugh unit and yolk percent.

HOT GAS HALOS IN EARLY-TYPE GALAXIES AND ENVIRONMENTS

  • Kim, Eunbin;Choi, Yun-Young;Kim, Sungsoo S.
    • Journal of The Korean Astronomical Society
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    • v.46 no.1
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    • pp.33-40
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    • 2013
  • We investigate the dependence of the extended X-ray emission from the halos of optically luminous early-type galaxies on the small-scale (the nearest neighbor distance) and large-scale (the average density inside the 20 nearest galaxies) environments. We cross-match the 3rd Data Release of the Second XMMNewton Serendipitous Source Catalog (2XMMi-DR3) to a volume-limited sample of the Sloan Digital Sky Survey (SDSS) Data Release 7 with $M_r$ < -19.5 and 0.020 < z < 0.085, and find 20 early-type galaxies that have extended X-ray detections. The X-ray luminosity of the galaxies is found to have a tighter correlation with the optical and near infrared luminosities when the galaxy is situated in the low large-scale density region than in the high large-scale density region. Furthermore, the X-ray to optical (r-band) luminosity ratio, $L_X/L_r$, shows a clear correlation with the distance to the nearest neighbor and with large-scale density environment only where the galaxies in pair interact hydrodynamically with seperations of $r_p$ < $r_{vir}$. These findings indicate that the galaxies in the high local density region have other mechanisms that are responsible for their halo X-ray luminosities than the current presence of a close encounter, or alternatively, in the high local density region the cooling time of the heated gas halo is longer than the typical time between the subsequent encounters.

Investigations into the Cylinder Flow Stabilities with a Thin Film Attachment

  • Doh, Deog-Hee;Jo, Hyo-Je;Kwon, Seang-Yong;Kim, Hyoung-June;Cho, Gyeang-Rae;Shin, Byeong-Rog
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.7
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    • pp.957-965
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    • 2011
  • The wakes of a cylindrical body have been investigated. The cylindrical body was attached with a thin film. The film is made of silicon with configurations of 50mm(W) ${\times}$ 150mm(L) ${\times}$ 0.3mm(T). The cylinder wakes have been measured with PIV experiments under the conditions with and without the thin film. The diameter of the installed cylinder body is 30mm and the Reynolds numbers are 2730, 6160 and 9750 with the diameter. The measurement system consists of an Ar-ion laser(6W), a high speed camera(1024 ${\times}$ 992 pixel, 500fps) and a host computer. FFT analyses have been carried out using the velocity vectors obtained by PIV measurements at the point X/D=1.52 and Z/D=0.52. For understanding the three-dimensional flow structures, a new Volumetric PTV(particle tracking velocimetry) has been constructed, in which the same four high-resolution cameras have been used. It has been verified that the flexible film suppresses or damps the vortices separated from the cylinder body, which makes the cylinder's wakes stable. With increase of Re numbers the intensity of the dominant frequency of the wakes become smaller.

Effects of Sex and Market Weight on Performance, Carcass haracteristics and Pork Quality of Market Hogs

  • Piao, J.R.;Tian, J.Z.;Kim, B.G.;Choi, Y.I.;Kim, Y.Y.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.10
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    • pp.1452-1458
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    • 2004
  • An experiment was conducted to examine the effects of sex and market weight on performance, carcass characteristics and pork quality. A total of 224 crossbred pigs (initially 26.64 kg BW) were allotted in a $2{\times}4$ factorial arrangement in a randomized complete block (RCB) design. The variables were sex (gilts and barrows) and different market weights (100, 110, 120 and 130 kg). Average daily gain (ADG) and average daily feed intake (ADFI) were significantly higher (p<0.01) in barrows than gilts, ADFI and feed conversion ratio (FCR) increased as body weight increased (p<0.05). Gender differences were observed in carcass characteristics. Backfat thickness and drip loss were greater in barrows (p<0.01), while loin eye area (p<0.01), flavor score (p<0.05) and lean content (p<0.001) were higher in gilts. Carcass grade and water holding capacity were the highest in 110 kg market weight pigs. The 100 kg arket weight pigs showed lower juiciness, tenderness, shear forces and total palatability than the other market weights (p<0.01). Hunter values (L*, a* and b*) were increased as market weight increased (p<0.05). Hunter a* value was greater in gilts (p<0.01) but L* value and b* value were not affected by sex of pigs. Net profit [(carcass weight${\times}$price by carcass grade)-(total feed cost+cost of purchased pig)] was higher in gilts than barrows (p<0.01), and was higher (p<0.05) in the pigs marketed at 110 and 120 kg market weight compared with 100 kg market weight. These results demonstrated that gilts showed higher carcass characteristics, pork quality, feed cost per kg body weight gain and net profit compared with barrows. Moreover, 110 or 120 kg body weight would be the recommended market weight based on pork quality and net profit for swine producers.

Mapping of Quantitative Trait Loci on Porcine Chromosome 7 Using Combined Data Analysis

  • Zuo, B.;Xiong, Y.Z.;Su, Y.H.;Deng, C.Y.;Lei, M.G.;Zheng, R.;Jiang, S.W.;Li, F.E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.10
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    • pp.1350-1353
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    • 2004
  • To further investigate the regions on porcine chromosome 7 that are responsible for economically important traits, phenotypic data from a total of 287 F2 individuals were collected and analyzed from 1998 to 2000. All animals were genotyped for eight microsatellite loci spanning the length of chromosome 7. QTL analysis was performed using interval mapping under the line-cross model. A permutation test was used to establish significance levels associated with QTL effects. Observed QTL effects were (chromosomewide significance, position of maximum significance in centimorgans): Birth weight (<0.01, 3); Carcass length (<0.05, 80); Longissimus muscle area (<0.01, 69); Skin percentage (<0.01, 69); Bone percentage (<0.01, 74); Fat depths at shoulder (<0.05, 54);Mean fat depth (<0.05, 81); Moisture in m. Longissimus Dorsi (<0.05, 88). Additional evidence was also found which suggested QTL for dressing percentage and fat depths at buttock. This study offers confirmation of several QTL affecting growth and carcass traits on SSC7 and provides an important step in the search for the actual major genes involved in the traits of economic interest.

Genetic Variation and Genetic Relationship of Seventeen Chinese Indigenous Pig Breeds Using Ten Serum Protein Loci

  • Mo, D.L.;Liu, B.;Wang, Z.G.;Zhao, S.H.;Yu, M.;Fan, B.;Li, M.H.;Yang, S.L.;Zhang, G.X.;Xiong, T.A.;Li, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.7
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    • pp.939-945
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    • 2003
  • Seventeen Chinese indigenous pig breeds and three introduced pig breeds had been carried out by means of vertical polyacrylamide gel electrophoresis (PAGE). According to the results, eight serum protein loci were highly polymorphic except Pi-2 and Cp. The polymorphism information content (PIC) of Hpx was the highest (0.5268), while that of Cp was the lowest (0.0257). The population genetic variation index showed that about 84% genetic variation existed in the population, and the rest of 16% distributed between the populations. The genetic variation of Yimeng black pig and Duroc were the highest and the lowest, respectively. The genetic variation of Chinese indigenous pig breeds was much more than that of exotic groups. Genetic distance results showed that Chinese indigenous pig breeds were classified into four groups with the three introduced pig breeds clustered into another group. The results also supported the geographic distribution of Chinese indigenous pig breeds in certain extent.

Effect of Monochromic Light-emitting Diode Light with Different Color on the Growth and Reproductive Performances of Breeder Geese

  • Chang, S.C.;Lin, M.J.;Zhuang, Z.X.;Huang, S.Y.;Lin, T.Y.;Jea, Y.S.;Fan, Y.K.;Lee, T.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.6
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    • pp.830-837
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    • 2016
  • The purpose of this study was to investigate the effect of monochromic light-emitting diode (LED) light with different color on the growth and reproductive performances of white Roman breeder geese. A randomized complete batch design was utilized for the trial, and the replicate was regarded as one batch. Twenty ganders and fifty-five dames were used in batch 1 (started on 2011/6/17 and ended on 2012/1/31), thirty ganders and eighty-four dames were used in batch 2 (started on 2012/3/23 and ended on 2012/10/26), and thirty ganders and seventy-two dames were used in batch 3 (started on 2013/3/12 and ended on 2013/12/20). Two hundred and ninety-one geese were randomly assigned to 6 rooms in an environmentally controlled house. They were randomly allotted into one of three monochromatic light treatments: Blue, red, or white. The results showed that there was no significant difference in body weight among the three lighting groups at any point throughout the experimental period. However, compared to the blue light group, significantly more eggs were produced by the red and white light groups (p<0.05). Furthermore, the laying period of the red light group was significantly longer than that of other two groups (p<0.05). In conclusion, our results suggested that red LED-light has the best effect on reproductive performance (i.e. longer laying period and higher total eggs number) at 30 lux light intensity, and is therefore a better choice for the management of breeding geese than blue or white LED-light.

Control of Methane Emission in Ruminants and Industrial Application of Biogas from Livestock Manure in Korea

  • Song, Man-K.;Li, Xiang-Z.;Oh, Young-K.;Lee, Chang-Kyu;Hyun, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.1
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    • pp.130-136
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    • 2011
  • Methane is known to be one of the major greenhouse gases. On a global scale, livestock farming may contribute 18% of total greenhouse gas emissions. Though methane contribution is less than 2% of all the factors leading to global warming, it plays an important role because it is 21 times more effective than carbon dioxide. Methane emission is a direct result of the fermentation process performed by ruminal microorganisms and, in particular, the archael methanogens. Reducing methane emission would benefit both ruminant production and the environment. Methane generation can be reduced by electron-sink metabolic pathways to dispose of the reducing moieties. An alternative way for methane control in the rumen is to apply inhibitors against methanogens. Generating methane from manure has considerable merit because it appears to offer at least a partial solution to two pressing problems-environmental crisis and energy shortage. An obvious benefit from methane production is the energy value of the gas itself. Control of methane emission by rumen microbes in Korea has mainly been focused on application of various chemicals, such as BES and PMDI, that inhibit the growth and activity of methanogens in the rumen. Alternatives were to apply long-chain polyunsaturated fatty acids and oils with or without organic acids (malate and fumarate). The results for trials with methane reducing agents and the situation of biogas production industries and a typical biogas plant in Korea will be introduced here.