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Studies on the Development of a Microbial Cryoprotectant Formulation Using a W/O/W Multiple Emulsion System

  • Bae, Eun-Kyung;Cho, Young-Hee;Park, Ji-Yong
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.673-679
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    • 2004
  • A microbial cryoprotectant formulation using a W/O/W multiple emulsion system was developed. The psychrotolerant microorganism, B4, isolated from soil in South Korea, was observed by the drop freezing method, in which the microorganism sample inhibited ice nucleation activity. The antifreeze activity was eliminated when the microorganism sample was treated with protease, indicating that the antifreeze activity was due to the presence of antifreeze protein. The result of the l6S rDNA sequencing indicated the B4 strain was most closely related to a species of the genus Bacillus. Culture broth of B4 strain (Bacillus sp.) and rapeseed oil containing 1 % polyglycerine polyricinolate (PGPR) were used as core and wall material, respectively. The most stable W/O emulsion was prepared at a core/oil ratio of 1:2. The highest W/O/W emulsion stability was achieved when the primary emulsion to external aqueous phase containing 0.5% caster oil polyoxyethylene ether $(COG25^{TM})$ ratio was 1:1. Microcrystalline cellulose showed better W/O/W emulsion stability than other polymer types. The viability of cells in a W/O/W emulsion was higher than free cells during storage at $37^\circ{C}$. An acidic pH and UV exposure decreased the viability of free cells, but cells in W/O/W emulsion were more stable under these conditions.

A Study on the 8W High Power Amplifier for VSAT at Ku-band (Ku-band의 소형 지구국용을 위한 8W 고출력 증폭기에 관한 연구)

  • 조창환;이찬주;홍의석
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.7 no.1
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    • pp.53-60
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    • 1996
  • The 8W hybrid MIC SSPA has been developed in the frequency range from 14.0 GHz to 14.5 GHz for uplink of KOREASAT's earth station. The whole system was designed of two parts with driving amplifier and high power amplifier to simplify the fabrication process. we reduced weight and volum of power amplifier through arranging the bias circuits in the same housing. The realized SSPA has a small signal gain of $26\pm1dB$within 500 MHz bandwidith, and the input and output return losses are over 7dB and 12dB respectively. The output power of 39.0 ~ 39.2dBm is achieved at the 1dB gain compression point of 14 GHz, 14.25 GHz, and 14.5 GHz. That reveals higher power than 8W of design target. The proposed SSPA manufacture techni- ques in this paper can be applied to the implementation of power amplifiers for some radars and SCPC.

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Effects of Plant Water Extract Codonopsis Lanceolatae on Mouse Immune Cell Activation Ex Vivo (더덕 물 추출물의 경구 투여가 마우스 면역 세포 활성에 미치는 효과)

  • Ryu, Hye-Sook;Kim, Kyoung-Ok;Kim, Hyun-Sook
    • Journal of Nutrition and Health
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    • v.42 no.3
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    • pp.207-212
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    • 2009
  • Codonopsis lanceolatae has been used as one of the traditional remedies as well as food source. However, few studies on their immunomodulating effects have been reported. We previously reported that ex vivo supplementation of Codonopsis lanceolatae water extracts enhanced splenocyte proliferation compared to the control group. In order to elucidate its ex vivo effect, six to seven week old balb/c mice were fed ad libitum on a chow diet and water extracts of Codonopsis lanceolatae were orally administrated every other day for four weeks at two different concentrations (50 and 500 mg/kg B.W). After preparing the single cell suspension, the proliferation of splenocytes was determined by MTT (3- [4,5-dimethylthiazol-2-y] -2,5-diphenyl terazolium bromide) assay. The production of cytokine (IL-1${\beta}$, IL-6, TNF-${\alpha}$), secreted by macrophages stimulated with LPS or not, was detected by ELISA assay using a cytokine kit. After 48 hrs of incubation with the mitogen (ConA or LPS) stimulation, the mice splenocyte proliferation in experimental group was statistically increased at two different concentrations than that in control group. The cytokines production was more significantly enhanced at the lower supplementation (500 mg/kg B.W.) group rather than higher concentration (500 mg/kg B.W.) compared to the control group. The results of this study may suggest that the supplementation of water extract of plant mixture could regulate the immune function by increasing the splenocyte proliferation and enhance the immune function through regulating cytokine production capacity by activated macrophages in mice.

Comparison of Combined Light-emitting Diodes and Fluorescent Lamps for Growth and Light Use Efficiency of Red Leaf Lettuce (혼합 발광다이오드와 형광등에서 자란 적치마 상추의 생육 및 광 이용 효율 비교)

  • Son, Ki-Ho;Song, Min-Jeong;Oh, Myung-Min
    • Journal of Bio-Environment Control
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    • v.25 no.3
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    • pp.139-145
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    • 2016
  • The objective of this study was to compare the growth and light use efficiency of red leaf lettuce grown under three types of combined light-emitting diodes (LEDs) and fluorescent lamps (FL) in a closed-type plant production system. The eighteen days-old lettuce seedlings of red leaf lettuce (Lactuca sativa L., 'Jeokchima') were transplanted to the close-type plant production system equipped with three types of combined LEDs with red (R, 655 nm), blue (B, 456 nm), green (G, 515 nm), and white (W, 456 nm + 558 nm) (R:B=8:2, R:W:B=8:1:1, R:G:B=8:1:1) and FL. The seedlings were grown under normal growth conditions ($20^{\circ}C$, $181{\pm}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 h photoperiod) for four weeks. Lettuce plants grown under FL had significantly higher leaf shape index than those under all LED treatments. Although growth of shoots and roots was not show any significant difference among LED treatments, all of the LED treatments induced about 34% higher shoot fresh weight than that of the FL. On the other hands, the total power consumption of FL was 145 kW for 4 weeks, while the mean value of LED treatments was 54 kW, which was about 3 times lower value than that of the FL. The light use efficiency based on dry matter in LED treatments was about 34 mg/W and this was about 3.5 times higher energy saving value than the FL. In conclusion, this study showed that irradiation of optimal combined LEDs in closed-type plant production systems can improve the lettuce growth as well as maximize in light use efficiency through energy saving than the FL.

Characteristics of Microwave-assisted Extraction for Catechins from Grape Seed (포도씨 카테킨류의 마이크로웨이브 추출특성)

  • Lee, Eun-Jin;Choi, Sang-Won;Kim, Hyun-Ku;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.40 no.5
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    • pp.510-515
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    • 2008
  • Microwave energy was applied to the extraction of functional catechins from grape seed. The solvent, absolute ethanol, reached the boiling point when exposed for less than 3 min microwave treatment at 100 W. The effects of independent variables in microwave-assisted extraction (MAE), including microwave power (0-160W, $X_1$), ethanol concentration (0-100%, $X_2$) and extraction time (1-5 min, $X_3$), were investigated on each response variable ($Y_n$), and the contents of catechin and its derivatives were determined via response surface methodology, thereby allowing us to predict their optimal extraction conditions. The predicted maximal values of (+)-catechin, procyanidin $B_2$, (-)-epicatechin, and (-)-epicatechin gallate were 137.99, 72.78, 222.38, and 9.59 mg%, respectively, under different MAE conditions. The predicted extraction conditions for maximum catechin responses were as follows: 104.10 W of microwave power, 45.35% of EtOH, and 4.89 min of extraction time for (+)-catechin (137.99 mg%), 133.16 W, 46.16% and 4.49 min for procyanidin $B_2$ (72.78 mg%), 136.00 W, 41.37% and 4.39 min for (-)-epicatechin (222.38 mg%), 143.20 W, 37.51% and 1.88 min for (-)-epicatechin gallate (9.59 mg%), respectively. The contents of (+)-catechin, procyanidin 1B2 and (-)-epicatechin in MAE were similarly influenced by three independent variables, whereas (-)-epicatechin gallate was influenced less profoundly by ethanol concentration and extraction time.

Effects of Glucosamine Hydrochloride, Taurine and Their Combined Administration on Anti-inflammatory and Analgesic Action in Rats (흰쥐의 항염증 및 진통작용에 대한 Glucosamine Hydrochloride와 Taurine 및 그 혼합 투여시의 효과)

  • 김옥경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1113-1123
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    • 1999
  • The purpose of this study was to see the effect of anti inflammatory and analgesic action of the glucosamine hydrochloride(GA HCl) or taurine. Male Sprague Dawley rats(100~250g) and ICR mice(20 ~30g) were used. Experimental groups were divided into seven groups, one control group given as saline and six groups given as oral administration of GA HCl or taurine; GA HCl 250mg/kg, b.w group, taurine 250mg/kg, b.w group, GA HCl 250mg/kg, b.w+taurine 250mg/kg, b.w group, GA HCl 500mg/kg, b.w group, taurine 500mg/kg, b.w group, GA HCl 500mg/kg, b.w+taurine 500mg/kg, b.w group. Carrageenan induced edema test were shown to be significantly inhibited in the GA HCl 250mg/kg group and taurine 250mg/kg group compared to the control group, but the GA HCl 500mg/kg+taurine 500mg/kg group were significantly inhibited than the control group. Capillary permeability test were shown to be sig nificantly inhibited in the taurine 500mg/kg group, but the GA HCl 500mg/kg+taurine 500mg/kg group were significantly inhibited than the control group. Leucocyte emigration test were shown to be significantly inhibited in the GA HCl 250mg/kg+ taurine 250mg/kg group and GA HCl 500mg/kg+taurine 500mg/kg group compared to the control group. Acetic acid, Phenyl p benzoquinone writhing syndrome were shown to be significantly inhibited in the GA HCl 500mg/kg+taurine 500mg/kg group compared to the control group. Inhibitory action against COX 1 and COX 2 were not significantly inhibited in the experimental group. These results suggest that the combined administration of the GA HCl and taurine have potential action in anti inflammatory and analgesic action.

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A 10bit 1MS/s 0.5mW SAR ADC with Double Sampling Technique (더블 샘플링 기법을 사용한 10bit 1MS/s 0.5mW 축차 비교형 아날로그-디지털 변환기)

  • Lee, Ho-Kyu;Kim, Moo-Young;Kim, Chul-Woo
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.60 no.2
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    • pp.325-329
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    • 2011
  • This paper introduces the 10b 1MS/s SAR ADC with double sampling technique to reduce the power consumption. The SAR ADC is implemented in CMOS 1P8M 65nm technology and occupies 0.11um2. The maximum sampling rate is 1MS/s. The simulated SNDR and SFDR are 55.6dB and 62.7dB at 484kHz input frequency, respectively. The implemented data converter consumes 507uW with 1.2-V supply.

RADIOLOGICAL STUDY OF THE CONDYLAR HEADS IN TEMPOROMANDIBULAR JOINT ARTHROSIS (악관절증에서의 하악과두의 방사선학적 소견)

  • You Dong Soo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.15 no.1
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    • pp.13-20
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    • 1985
  • The author obtained the oblique lateral trans cranial radiograms from 376 patients (114 of male, and 262 of female) with temporomandibular joint arthrosis. After tracing each film, the author analyzed the dimensional changes of the condylar heads with pain, clicking, mouth opening limitation, and masticatory difficulty respectively, which were the chief complaints of the TMJ arthrosis and compared these data with control group. The results were as follows; 1. There was a great predilection for occurrence of the TMJ arthrosis in female (262 cases) over male (114 cases). But there was no significant difference in ratio between the sexes on each symptom. In male, 60 patients (52.6%) had pain, 28 patients (24.6%) had clicking, 21 patients (18.4%) had mouth opening limitation, and 5 patients (4.4%) had masticatory difficulty. In female, 148 parients (56.5%) had pain, 57 patients (21.8%) had clicking, 47 patients (17.9%) had mouth opening limitation, and 10 patients (3.8%) had masticatory difficulty. 2. Examined controlled group, the author analyzed the dimension of W-W', 0-H, 0-A, 0-B on the right side first, and left side second. In male, the dimension of W-W' was 14.52㎜, 14.13㎜; 0-H was 13.92㎜, 13. 71㎜' 0-A was 8.91㎜, 9.0㎜ and 0-B was 8.67㎜, 8.78㎜. In female, W-W' was 13.77㎜, 13.51㎜; 0-H was 13.42㎜, 13.35㎜; 0-A was 8.92㎜, 9.01㎜; and 0-B was 8.59㎜, 8.80㎜. 3. W-W' and 0-H of the experimental group were distinctly lesser than the controlled group. Male with mouth opening limitation show the least (12.70㎜, 13.00㎜) on W-W', but both sexes with pain show the least on 0-H. There was no significant difference between experimental group with Clicking and controlled group on 0-H, 0-A, and 0-B. And also there was no difference on 0-B in patients with every symptom except masticatory difficulty. The dimensional changes of the condylar heads with those symptoms were as follows; a) In male patients, complaing of pain, W-W' of the right and left was 13.80㎜, 12.80㎜; 0-H was 13.10㎜, 12.90㎜; 0-A was 8.69㎜, 8.18㎜; 0-B was 8.33㎜, 8.42㎜. In female, W-W' was 13.01㎜, 12.90㎜; 0-H was 12.48㎜, 12.80㎜; 0-A was 8.60㎜, 8.49㎜; 0-B was 8.48㎜, 8.50㎜. b) In male patients with clicking, W-W' was 13.70㎜, 13.10㎜; 0-H was 13.90㎜, 13.10㎜; 0-A was 8.81㎜, 8.16㎜ 0-B was 8.34㎜, 8.25㎜. In female, W-W' was 13.10㎜, 13.50㎜; 0-H was 13.30㎜, 12.91㎜; 0-A was 8.95㎜, 8.49㎜; 0-B was 8.23㎜, 8.70㎜. c) In male patients with mouth opening limitation, W-W' was 12.70㎜, 13.00㎜; 0-H was 13.40㎜, 13.40㎜; 0-A was 8.37㎜, 8.48㎜; 0-B was 8.33㎜, 8.62㎜. In female, W-W' was 13.00㎜, 12.50㎜ 0-H was 12.90㎜, 13.10㎜; 0-A was 8.49㎜, 8.09㎜; 0-B was 8.77㎜, 8.01㎜. d) In male patients with masticatory difficulty, W-W' was 13.30㎜, 13.20㎜; 0-H was 13.40㎜, 12.60㎜; 0-A was 8.26㎜, 8.32㎜; 0-B was 7.80㎜, 8.20㎜. In female, W-W' was 12.30㎜, 12.00㎜; 0-H was 13.10㎜, 13.20㎜; 0-A was 8.68㎜, 7.95㎜; 0-B was 7.46㎜, 7.87㎜.

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Influence of Weft's Cotton Count & Weave Construction on the Mechanical Properties & Hand of Cotton Woven Fabrics (위사번수와 조직이 면직물의 역학특성 및 태에 미치는 영향)

  • Bae, Jin-Hwa;Park, Jung-Whan;An, Seung-Kook
    • Fashion & Textile Research Journal
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    • v.7 no.5
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    • pp.553-559
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    • 2005
  • Hand characteristics related with structural properties of fabrics have something to do with mechanical properties of fabric. In this study, the mechanical properties and hand characteristics have been analyzed according to fabric structural parameters such as the weave structure and the linear density of weft of cotton fabric. Mechanical properties have been used by KES-FB system which measures hand characteristics and mechanical properties of fabric. Linear density of weft, tensile, bending, and shear properties are decreasing with increasing weft linear density, and there is no considerable effects on compression and surface properties. In case of formability with weft linear density, B/W, 2HG/G, 2HB/B, 2HB/W, $\sqrt[3]{B/W}$, $\sqrt{2HB/W}$, W/T except MMD/SMD, WC/T, and WC/W have been effected. There is a high correlation between the crimp, tightness, hand, formability and mechanical properties specially tensile linearity, bending, shear, and compression properties. The weft crimp influences the bending rigidity, shear properties, and the tightness which have effects on the tensile linearity, bending, shear, compression properties, hand, and formability.

쌍끌이 중층트롤어법의 연구 ( 2 ) - 모형어구의 깊이에 관하여 - ( A Study on the Pair Midwater Trawling ( 2 ) - Working Depth of the Model Net - )

  • 이병기
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.31 no.1
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    • pp.45-53
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    • 1995
  • Working depth of the model net was determined by using of the same experimental tank and the same model net that used in the forwarded report in a series studies. The depth of the net which indicates the depth of the head rope from the water surface, was determined by the photographs taken in front of the net mouth with the combination of towing velocity, warp length and distance between paired boats. The results obtained can be summarized as follows: 1. Working depth of model nets A and B was varied in the range of 0.09~1.66$m$,and 0.04~1.34$m$(which can be converted into 2.7~40.2$m$and 1.2~49.8$m$in the full-scale net) respectively, and the depth of model net A was slightly deeper than the depth of the model net B. 2. Working depth ($D$,which is appendixed m for the model net, f for the full-scale net, A and B for the types of the model nets) can be expressed as the function of towing velocity$V_t$, as in the model net($V_t$=$m$/$sec$) $D_{mA}$=(-1.99+0.65$L_w$) $e^{-1.72V_t}$ $D_{mA]$=(-1.91+1.04 $L_w$) $e^{2.88V_t}$ in the full-scale net($V_t$=$k$'$t$ $D_{fA}$=(-29.32+0.65$L_w$)$e^{0.40 V_t}$ $D_{fB}$=(-57.60+1.04$L_w$)$e^{-0.67 V_t}$ 3. Working depth 9$D$ appendixes are as same as the former) can be expressed as the function of warp length$L_w$) in the model net, and can be converted into full-scale net as in the model net ($V_t$=$m$/$sec$) $D_{mA}$=-0.99 $e^{-1.42V_t}$+0.67$e^{-1359V_t}$$L_w$ $D_{mB}$=-.258$e^{-3.77V_t}$+1.16$e^{-3.15V_t$ $L^w$, in the full-scale net($V_t$=k't) $D_{fA}$=-29.28$e^{-0.32V_t}$+0.67$e^{-0.37V_t$$L_w$ $D_{fB}$=-69.10$e^{-0.81V_t}$+1.16$e^{-0.72V_t}$$L_w$. 4. Working depth was gradually shallowed according to the increase of the distance between paired boats.

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