• Bulletin of the Korean Chemical Society
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• v.30 no.9
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• pp.1981-1984
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• 2009

Subcellular localization of protein kinase often plays an important role in determining its activity and specificity. Protein kinase C (PKC), a family of multi-gene protein kinases has long been known to be translocated to the particular cellular compartments in response to DAG or its analog phorbol esters. We used C-terminal green fluorescent protein (GFP) fusion proteins of PKC isoforms to visualize the subcellular distribution of individual PKC isoforms. Intracellular localization of PKC-GFP proteins was monitored by fluorescence microscopy after transient transfection of PKC-GFP expression vectors in the HeLa cells. In unstimulated HeLa cells, all PKC isoforms were found to be distributed throughout the cytoplasm with a few exceptions. PKC$\theta$ was mostly localized to the Golgi, and PKC$\gamma$, PKC$\delta$ and PKC$\eta$ showed cytoplasmic distribution with Golgi localization. DAG analog TPA induced translocation of PKC-GFP to the plasma membrane. PKC$\alpha$, PKC$\eta$ and PKC$\theta$ were also localized to the Golgi in response to TPA. Only PKC$\delta$ was found to be associated with the nuclear membrane after transient TPA treatment. These results suggest that specific PKC isoforms are translocated to different intracellular sites and exhibit distinct biological effects.

• 한국정보디스플레이학회:학술대회논문집
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• 2004.08a
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• pp.507-510
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• 2004

Oblique ion-induced secondary electron emission coefficient(${\gamma}$) with low energy ..and work function ${\phi}_{\omega}$(${\theta}$ = 0 and ${\theta}$ = 20) of the MgO thin film in AC-PDPs has been measured by ${\gamma}$-FIB system. The MgO thin film has been deposited from sintered material under electron beam evaporation method. The energy of $He^+$ ions used has been ranged from 50eV to 150eV. Oblique ion beam has been chosen to be 10 degree, 20 degree and 30 degree. It is found that the higher secondary electron emission coefficient(${\gamma}$) has been achieved by the higher oblique ion beam up to inclination angle of 30 degree than the perpendicular incident ion beam.

• Journal of Advanced Marine Engineering and Technology
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• v.29 no.1
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• pp.17-24
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• 2005

The characteristics of diesel spray have effect on the engine Performance such as power. fuel consumption and emissions. Therefore, This study was Performed to investigate the effect of various injection parameters. In this study. the experiment is performed by using the high temperature and high pressure chamber. Spray behaviors are visualized by using the high speed camera and spray angle. Penetration etc. are measured. Experimental results are summarized as follows ; 1) Correlations of spray Penetration is expressed as follows $$0 $$t_b 2) Correlations of spray Angle is expressed as follows $$T_a=293K \;;\; tan({\theta}/2)=0.59({\rho}_a/{\rho}_f)^{0.437}$$ $$T_a=473K\;;\; tan({\theta}/2)=0.588({\rho}_a/{\rho}_f)^{0.404}$$ 3) The measured macro characteristics - spray tip penetration and spray angle agreed well with established correlations.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.18 no.9
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• pp.698-706
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• 2006

Experiment and numerical calculation have been peformed to investigate mixed convection heat transfer between inclined parallel plates. Particle image velocimetry (PIV) with thermo-sensitive liquid crystal (TLC) tracers is used for visualizing and analysis. This method allows simultaneous measurement of velocity and temperature fields at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. The governing equations are discretized using the finite volume method. The results are presented for the Reynolds number ranges from 0.004 to 0.062, the angle of inclination, ${\Theta}$, from 0 to 45 degree and Prandtl number of the high viscosity fluid is 909. The results show velocity, temperature and mean Nusselt numbers distributions. It is found that the periodic flow of mixed convection between inclined parallel plates is shown at $0^{\circ}{\leq}{\Theta}<30^{\circ}$, Re<0.062, and the flow pattern can be classified into three patterns which depend on Reynolds number and the angle of inclination. The minimum Nusselt numbers occur at Re=0.05 regardless of the angle of inclination.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.7 no.1
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• pp.108-116
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• 1997

A laboratory experiment was made of critical transition flow modes in Czocllralski convection. Numerical computation was also made to delineate the dynamic transition. The period of temperature oscillation ($t_p$) and the interval of temperature oscillation ($\Delta\theta$) were scrutinized to capture the critical transition regime. The mixed convection parameter was varied in the range of $0.134\le Ra/PrRe^2 \le3.804$. The data from calculation were in good agreement with ones from experiment. The influence of the Prandtl number on the transition was examined for Pr ＝ 910, 4445 and 8889. To understand the transition mechanism, the detailed temperature oscillation modes, the isolines of meridional temperature and the axial velocity profiles were investigated.

• Journal of the Korean Society of Costume
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• v.64 no.4
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• pp.118-130
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• 2014

The purpose of this study is to measure the touching stimulus level of EEG for fabrics to create a reference data for product planning of clothes with touching characteristics of high satisfaction. The subjects were composed of 6 female college students and their EEG level was measured while they touched 6 kinds of Korean traditional silk fabric. The results are as follows: 1. Shantung and Myoungju showed the highest and the lowest values of alpha for the touching stimulus respectively. The fabric of Shantung showed high values of alpha, beta, theta, delta, gamma, high-beta and SMR for the touching stimulus. 2. The values of beta were the highest with Shantung and the lowest with Nobangju for the touching stimulus. 3. The highest values of theta and delta for the touching stimulus was assigned to Shantung and the lowest belonged to Gabsa. 4. The highest values of gamma for the touching stimulus was assigned to Shantung and the lowest belonged to Gongdan. 5. The highest value of SMR for the touching stimulus was assigned to Santung, and the lowest belonged to Gongdan and Nobangju. 6. The highest values of high-beta for the touching stimulus was assigned to Shantung and the lowest belonged to Gabsa and Gongdan.

• Journal of the korean Society of Automotive Engineers
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• v.4 no.3
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• pp.56-68
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• 1982

This paper presents analytic solutions of defection and their resonance diagrams for a uniform beam of infinite length subjected to an constant axial force and moving transverse load simultaneously. Steady solutions are obtained by a time-independent coordinate moving with the load. The supporting foundation includes damping effects. The influences of the axial force, the damping coefficient and the load velocity on the beam response are studied. The limiting cases of no damping and critical damping are also investigate. The profiles of the deflection of the beam are shown graphically for several values of the load speed, the axial force and damping parameters. Form the results, following conclusions have been reached. 1. The critical velocity .THETA.cr decreases as the axial compressive force increases, but increases as the axial tensile force increase. 2. At the critical velocity .THETA.cr the deflection have a tendency to decrease as the axial tensile force increases and to increase gradually as the axial compressive force increases. 3. In case if relatively small dampings, the deflection increases suddenly as the velocity of the moving load approaches the critical velocity, and it reachs its maximum at the critical velocity, and it decreases and become greatly affected by the axial force as the velocity increases further. 4. in case of relatively large dampings, as the velocity increases the deflection decreases gradually and it is affected little by the axial load.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.1 s.94
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• pp.87-95
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• 2005

This paper proposes a method to calculate the characteristics of a coupled hydrodynamic journal and thrust bearing of a HDD spindle motor. The governing equations for the journal and thrust bearings are the two dimensional Reynolds equations in $\theta z$ and $ r\theta$ planes, respectively. Finite element method is appropriately applied to analyze the coupled journal and thrust bearing by satisfying the continuity of mass and pressure at the interface between the journal and thrust bearings. The pressure in a coupled bearing is calculated by applying the Reynolds boundary condition and compared with that by using the Half-Sommerfeld boundary condition. The static characteristics are obtained by integrating the pressure along the fluid film. The flying height of spindle motor is measured to verify the proposed analytical result. This research shows that the proposed method can describe HDB in a HDD system more accurately and realistically than the separate analysis of a journal or thrust bearing.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.9 s.174
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• pp.147-154
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• 2005

A precision hi-directional actuator for a high precision leveling system with $Z{\Theta}_x{\Theta}_y$ motions is proposed and designed in this paper. The actuator is composed of a force generation structure, a guide mechanism, and a symmetric structure. At first, its driving force is generated by a change of flux in air gaps by permanent and changeable flux. The permanent flux is generated by a permanent magnet. The changeable flux is created by variable current flowing through coil. The combination of permanent and changeable flux makes various flux densities in air gaps between moving part and fixed yokes. And then, the difference between flux densities in lower and upper gaps creates forces fur the $bi-direction({\pm}z)$ motion. The guide mechanism of this actuator is composed of two circular plates and one shaft. Reducing motions generated by forces except z-motion, these circular plates endow the actuator with high stiffness for fast settling time. And the function of the shaft is to transfer motion to an object. At last, total body has a symmetric structure to be stable on thermal error. The actuator is designed by MAXWELL 2D and ProMECHANICA. The designed actuator is evaluated by 8nm laser doppler vibrometer, dynamic signal analyzer, and simple PID controller.

• Journal of the Korean Society for Precision Engineering
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• v.19 no.2
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• pp.87-94
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• 2002

Most of Rapid Prototyping (RP) process adopt a solid Computer Aided Design (CAD) model, slicing into thin layers of uniform, but not necessarily constant, thickness in the building direction. Each cross-sectional layer is successive1y deposited and at the same time, bonded onto the previous layers; the stacked layers form a physical part of the model. The objective of this study is to develop a method for calculating the rotation angle ($$\theta$_x, $\theta$_y$) of hotwire of the cutting system in the three-dimensional space for the Variable Lamination Manufacturing process using expandable polystyrene foam sheet (VLM-S). In order to examine the applicability of the developed method to VLM-S, various three-dimensional shapes. such as a screw, an extruded cross, and free surface bodies such as miniatures of the monkey(a figure of Sonokong), were made using the data obtained form the method.