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Studies on the Antioxidative Effect of the Buckwheat (Fagopyrum esculentum Moench) Extract and its Protective Role against Cadmiun-mediated Stress (메밀의 항산화 및 카드뮴 방어 효능에 관한 연구)

  • Yoon Chang-Soon;Kim Nam-Hyoung;Jang Jae-Hyung;Sang Kyung Jin;Ko In Young;Choi Shin Wook
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.2 s.51
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    • pp.197-206
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    • 2005
  • In the present study, the Chuncheon buckwheat extracts prepared from its seed coats, seeds and stems were used to determine anti-oxidative effects, the content of rutin and phytic acid, and the protective role against cadmium at the cellular level. futhermore, it was evaluated whether the buckwheat, mainly known as a healthy food source, might be applicable to functional cosmetics. Up to $100 {\mu}g/mL$ of the extract was not toxic in HaCaT and B16F10 cell lines using MTT assay. The anti-oxidative capacity of superoxide radicals was shown in seed coats extracts > stem extracts=seed extracts. Although its content of rutin, known as one of effective anti-oxidants, mainly exists in the stem, any extract did not eliminate hydroxyl radicals. Phytic acid, known as a heavy metal-chelate agent, was highly concentrated in the stem. The Chuncheon buckwheat extract had $10\%$ protective effect against the treatment of $50{\mu}M$ cadmium at which $50\%$ of HaCaT cells survived. Confocal laser scanning microscope revealed the intracellular generation of reactive oxygen species (ROS) by cadmium treatment. Finally, we identified that the stem extract had the most protective effect on the elimination of ROS.

Characteristics of Seed Germination and Seedling Growth of Native Hydrangea serrata for. acuminata (자생 산수국의 종자 발아와 유묘 생육 특성)

  • Lee, Seung Youn;Kim, Kwang Jin;Lee, Jeong Sik
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.2
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    • pp.134-142
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    • 2008
  • This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).

A Study on the Evaluation of Culinary Major Selection Attributes Using IPA (IPA를 활용한 조리전공 선택속성 평가에 관한 연구)

  • Yang, Hyun-Kyo;Koo, Kyung-Won
    • The Journal of the Korea Contents Association
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    • v.21 no.1
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    • pp.417-425
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    • 2021
  • This study was conducted to evaluate the characteristics of major selection of college students majoring in culinary. By conducting an Importance-Performance Analysis(IPA) through students who are currently majoring in a culinary major, it is intended to increase student satisfaction, student loyalty, the enrollment rate and to present the direction the college should pursue. The questionnaire was conducted for 4 weeks from June 22, 2020 to July 19, 2020, the results are as follows. As a result of the t-test (paired sample t-test) for 23 attributes, the average value of importance was 4.0765, the average value of satisfaction was 3.5091, showing high importance, the attributes considered important by item were 'educational facilities (4.50)', 'school welfare (4.50)', the attributes having the highest satisfaction with experience after selecting a major were 'aptitude and conformity (3.94)', 'future hope and concordance (3.91)'. The IPA analysis results on the major selection attributes of college students majoring in culinary are as follows. First, In the first quadrant, 11 attributes including 'aptitude and conformity' appeared, Second, In the second quadrant, 5 attributes including 'employment support' appeared. Third, In the third quadrant, 5 attributes including 'college scholastic ability score' appeared, Finally, In the fourth quadrant, 2 attributes including 'experience in major field' appeared.

Anti-skin aging activities of ethanol extract from Echinodorus cordifolius L. in human keratinocytes (물수선화 에탄올 추출물의 피부 노화 억제 효과)

  • Haeun Mun;Seung-Hong Lee
    • Journal of Applied Biological Chemistry
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    • v.65 no.4
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    • pp.405-412
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    • 2022
  • Echinodorus cordifolius (L.) is an aquatic plant in the family Alismataceae. The anti-skin aging activity of E. cordifolius (L.) has not been yet reported. Therefore, the objective of the present study was to prepare 70% ethanol extract (ECEE) from E. cordifolius (L.) and investigate their antioxidant and anti-hyaluronidase activities for confirm the potential of anti-skin aging. ECEE showed good activities of DPPH, hydrogen peroxide scavenging, and hyaluronidase inhibition, with EC50 and IC50 values of 31.4, 300, and 450 ㎍/mL, respectively. ECEE also significantly improved cell viability and inhibited intracellular reactive oxygen species dose-dependently against 1 mM hydrogen peroxide-induced oxidative stress in immortalized human keratinocytes (HaCaT cells). Furthermore, ECEE upregulated hyaluronic acid (HA)-synthesizing enzyme hyaluronan synthase 2 (HAS2) expression level, but downregulated expression level of HA-degrading enzyme hyaluronidase 2, resulting in increased HA production in HaCaT cells. Taken together, these results suggest that ECEE shows antioxidant and anti-hyaluronidase potential and could be a functional cosmetic ingredients for anti-skin aging.

A Fundamental Study for Development of Unglazed Transpired Collector Control System in Windowless Pig House (무창돈사 내 무창기공형 집열기 제어 시스템 개발을 위한 기초적 연구)

  • Moon, Byeong Eun;Kim, Hee Tae;Kim, Jong Goo;Ryou, Young Sun;Kim, Hyeon Tae
    • Journal of agriculture & life science
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    • v.50 no.2
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    • pp.175-185
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    • 2016
  • In this study, manufactured experimental pig house (two pig house) and compared the changes in internal temperature and energy depending on the application of UTC control system for their utilizing of them as basic data for maintaining proper conditions for feeding environment and reducing heating energy depending on the UTC control system and program development, prior to applying the UTC system into pig house, representative agricultural facility. The control system ranges T1~T4 which is made to control a total of five output signals O1~O5 in the way of On/Off by using the algorithms of the program after measuring temperature scored 4 of total. Temperature setting was controlled with 28.0℃ in experimental pig house and 34.0℃ in UTC plenum, and output signal was controlled by comparing it with the measured temperature. During 3 days, the maximum temperature were measured at an average 31.8℃ when operated the control system in pig house. At the same time, the maximum temperature were measured 36.6℃ in comparison pig house, it was low temperature at 4.8℃ in experimental pig house than comparison pig house. Also, UTC plenum temperature was showed that rose at an average 50.5℃ by operation of the control program.

Growth Environment and Vegetation Structure of Habitats of Acer tegmentosum Maxim. (산겨릅나무(Acer tegmentosum Maxim.) 자생지의 서식환경 특성 및 식생구조)

  • Son, Ho-Jun;Kim, Se-Chang;Lee, Da-Hyun;Kwon, Soon-Jae;Park, Wan-Geun;Kim, Young-Seol
    • Journal of agriculture & life science
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    • v.50 no.3
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    • pp.69-80
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    • 2016
  • The present study was to survey the site environment, vegetation structure and soil characteristics in the wild habitats of Acer tegmentosum Maxim. and offers basic information for habitats conservation and restoration. Most of the wild habitats were located at altitudes between 605~1,413m with inclinations ranged as 8~30°. The bare rock rate were 8~50%. The vegetation structure by the PC-ORD based on the Two Way Cluster Analysis were divided into three groups Community I(Acer tegmentosum - Quercus mongolica), Community II(Acer tegmentosum - Carpinus cordata), Community III(Quercus mongolica - Tilia amurensis). The species diversity(H') was highest in Community II as 1.474, Community I was 1.471, Community III was 1.219. The soil textures were Clay loam, The average soil pH was 4.8, Soil organic matter was 15.15% and available phosphorus was 2.33ppm. Ordination analysis result by soil characteristics, community, characteristic species showed that the greatest effect factors were slope, altitude, tree and shrub's cover rate, organic matter, total-nitrogen, calcium, magnesium. Correlation analysis between environment factor result showed that O.M. - (T.N., K+, Mg2+, CEC, EC), T.N. - (K+, Mg2+, CEC, EC) were positive correlations.

Evaluation of Disturbance Effect of Penetrometer by Dissipation Tests (소산 실험을 이용한 관입 장비의 교란 효과 추정)

  • Yoon, Hyung-Koo;Hong, Sung-Jin;Lee, Woojin;Lee, Jong-Sub
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.6C
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    • pp.339-347
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    • 2008
  • The penetration of the probe produces the excess pore pressure due to the disturbance. The objective of this study is to evaluate the disturbance zone by using the dissipation of the excess pore water pressure, which was generated due to the penetration of the penetrometer with different size. The CPT, DMT and FVP (Field Velocity Probe) are adopted for in-situ tests. The tests are carried out in the construction site of north container pier of Busan new port, Korea where is accelerating the consolidation settlement using plastic board drains (PBD) and surcharges by crushed gravels. The coefficient of consolidation $(C_h)$ and soil properties are deduced by the laboratory test. The in-site tests are performed after the predrilling the surcharge zone at the point of 90% degree of consolidation. To minimize the penetration effect, the horizontal distance between penetration tests is 3m, the change of the pore pressure is monitored at the fixed depth of 24m. The coefficient of consolidation $(C_h)$ and the $t_{50}s$ are calculated based on the laboratory test and the in-situ data, respectively. The equvalent radi based on the $t_{50}$ shows that the FVP and the DMT produce the smallest and the greatest equivalent radi, respectively.

Various MRI Findings of Toxocara canis Myelitis (개회충 척수염의 다양한 자기공명영상 소견)

  • Sun Young Park;Kyung Seok Park;Eugene Lee;Joon Woo Lee;Yun Jung Bae;Yusuhn Kang;Joong Mo Ahn;Heung Sik Kang
    • Journal of the Korean Society of Radiology
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    • v.81 no.1
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    • pp.135-146
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    • 2020
  • Purpose To describe magnetic resonance imaging (MRI) findings in 10 cases of Toxocara canis myelitis and to analyze these findings to aid in the diagnosis of this condition. Materials and Methods From June 2015 to March 2018, we retrospectively analyzed the electronic medical records and MR images of patients who were diagnosed with Toxocara canis myelitis. The analysis of the MR images was based on a discussion between an experienced spinal radiologist and a radiology resident. Results This study classified MRI findings into the following two types. Type 1 was defined as central and diffuse T2 high signal intensity on the axial plane, which was observed in 50% of all cases. All lesions showed avid enhancement, mostly in the posterolateral or posterior region (4 cases, 80%). Type 2 was defined as wedge-shaped or focal T2 high signal intensity in the posterolateral or posterior region and corresponded to the remaining 50% of the cases. In this case, the extent of the lesion was relatively small and contrast enhancement was observed in only one case. Conclusion This study revealed that various imaging findings could be observed in cases of Toxocara canis myelitis, and the findings were classified into two types.

Effects of a Mixed Proportion of Alaska Pollack, Chicken Breast Surimi and Starch on Textural Properties of Sausage (명태 및 닭가슴살수리미와 전분의 혼합비율이 소시지의 조직학적 특성에 미치는 영향)

  • Jin, Sang-Keun;Kim, Il-Suk;Yang, Mi-Ra;Hur, In-Chul;Jung, Hyun-Jung;Yang, Han-Sul;Choi, Yeung-Joon
    • Journal of Animal Science and Technology
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    • v.50 no.4
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    • pp.543-550
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    • 2008
  • This study was conducted to determine the effect of different proportions of Alaska pollack surimi, chicken breast surimi and starch on textural properties of sausage. Sausage were manufactured such as Alaska pollack surimi(control), T1(90.91:0:9.09), T2(80:0:20), T3(62.5:37.5:0), T4(58.82:35.3:5.88), T5 (40:60:0) and T6(36.36:54.55:9.09) depending on different proportions of Alaska pollack surimi, chicken breast surimi and starch. Brittleness, breaking force and gel strength were significantly higher in T1 than the control(P<0.05), and breaking force, gel strength and folding test were significantly higher but sensory evaluation was significantly lower in T2 than the control(P<0.05). Breaking force, gel strength and jelly strength were significantly higher in T3 and T4 than the control(P<0.05). Gel characteristics and sensory scores were significantly higher but texture properties were significantly lower in T5 and T6 than the control(P<0.05). The T3 and T4 had similar textural and sensory properties in the surimi- sausage compared to the control. Therefore, the results of this study indicated that textural properties of sausage can be acceptable when chicken breast surimi replaced Alaska pollack up to 35%.

Comparison of the Diagnostic Accuracies of 1.5T and 3T Stress Myocardial Perfusion Cardiovascular Magnetic Resonance for Detecting Significant Coronary Artery Disease

  • Min, Jee Young;Ko, Sung Min;Song, In Young;Yi, Jung Geun;Hwang, Hweung Kon;Shin, Je Kyoun
    • Korean Journal of Radiology
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    • v.19 no.6
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    • pp.1007-1020
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    • 2018
  • Objective: To compare the diagnostic performance of cardiovascular magnetic resonance (CMR) myocardial perfusion at 1.5- and 3-tesla (T) for detecting significant coronary artery disease (CAD), with invasive coronary angiography (ICA) as the reference method. Materials and Methods: We prospectively enrolled 281 patients (age $62.4{\pm}8.3$ years, 193 men) with suspected or known CAD who had undergone 1.5T or 3T CMR and ICA. Two independent radiologists interpreted perfusion defects. With ICA as the reference standard, the diagnostic performance of 1.5T and 3T CMR for identifying significant CAD (${\geq}50%$ diameter reduction of the left main and ${\geq}70%$ diameter reduction of other epicardial arteries) was determined. Results: No differences were observed in baseline characteristics or prevalence of CAD and old myocardial infarction (MI) using 1.5T (n = 135) or 3T (n = 146) systems. Sensitivity, specificity, positive and negative predictive values, and area under the receiver operating characteristic curve (AUC) for detecting significant CAD were similar between the 1.5T (84%, 64%, 74%, 76%, and 0.75 per patient and 68%, 83%, 66%, 84%, and 0.76 per vessel) and 3T (80%, 71%, 71%, 80%, and 0.76 per patient and 75%, 86%, 64%, 91%, and 0.81 per vessel) systems. In patients with multi-vessel CAD without old MI, the sensitivity, specificity, and AUC with 3T were greater than those with 1.5T on a per-vessel basis (71% vs. 36%, 92% vs. 69%, and 0.82 vs. 0.53, respectively). Conclusion: 3T CMR has similar diagnostic performance to 1.5T CMR in detecting significant CAD, except for higher diagnostic performance in patients with multi-vessel CAD without old MI.