• BMB Reports
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• 제28권3호
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• pp.265-270
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• 1995

Acetolactate synthase (ALS, EC 4.1.3.18) is the first common enzyme in the biosynthesis of leucine, isoleucine, and valine. It is the target enzyme for several classes of herbicides, including the sulfonylureas, the imidazolinones, the triazolopyrimidines, the pyrimidyl-oxy-benzoates and the pyrimidyl-thio-benzens. The sulfonylurea-resistant ALS gene (SurB) from Nicotiana tabaccum [Lee et al. (1988) The EMBO J. 7, 1241-1248] was cloned into the bacterial expression plasmid pT7-7. The resulting recombinant plasmid pT7-ALS was used to transform an ALS-deficient Escherichia coli strain MF2000. MF2000 cells transformed with pT7-ALS grew in the absence of valine and isoleucine. ALS activities of 0.042 and 0.0002 ${\mu}mol/min/mg$ protein were observed in the crude extracts prepared from MF2000 cells transformed with plasmids pT7-ALS and pT7-7, respectively. In addition, the former crude extract containing mutant ALS was insensitive to inhibition by K11570, a new chemical class of herbicides. $IC_{50}$ values for K11570 were $0.13{\pm}0.01$ mM. For comparison, a plasmid pTATX containing the wild-type Arabidopsis thaliana ALS coding sequences was also expressed in MF2000. ALS activities of 0.037 ${\mu}mol/min/mg$ protein were observed, and the wild type ALS was sensitive to two different classes of herbicides, K11570 and ALLY, a sulfonylurea. $IC_{50}$ values for K11570 and ALLY were $0.63{\pm}0.07$ and $80{\pm}5.6$ nM, respectively. Thus, the results suggest that the sulfonylurea-resistant tobacco ALS was functionally expressed in the bacteria, and that K11570 herbicides bind to the regulatoty site of ALS enzymes.

• 대기
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• 제24권3호
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• pp.379-390
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• 2014

This study explores the long-term trends of surface air temperatures in 11 KMA stations over the period of 1960~2012. Both linear and nonlinear trends are examined for the $95^{th}$, $50^{th}$, and $5^{th}$ percentiles of daily maximum ($T_{max}$) and minimum temperatures ($T_{min}$) by using quantile regression method. It is found that in most stations linear trends of $T_{max}$ and $T_{min}$ are generally stronger in winter than in summer, and warming trend of the $5^{th}$ percentile temperature (cold extreme) is stronger than that of the $95^{th}$ percentile temperature (warm extreme) in both seasons. The nonlinear trends, which are evaluated by the second order polynomial fitting, show a strong nonlinearity in winter. Specifically, winter temperatures have increased until 2000s but slightly decreased afterward in all percentiles. This contrasts with the $95^{th}$ and $50^{th}$ percentiles of summer $T_{min}$ that show a decreasing trend until 1980s then an increasing trend. While this result is consistent with a seasonal dependence of the recent global warming hiatus, most of the nonlinear trends are statistically insignificant, making a quantitative attribution of nonlinear temperature trends challenging.

• 한국수산과학회지
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• 제38권4호
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• pp.251-256
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• 2005

For blood cockle Tegillarca granosa acclimated to winter and summer seasons, survival, temperature tolerance and physiological changes at different individual size were investigated for their temperature tolerances by increasing and decreasing temperature at a rate of 1, 2 and $3^{\circ}C/day$. The survival rate of adults and juveniles T. granosa acclimated to winter temperatures began to decrease from $32^{\circ}C$ and all experimental animals died between $37-39^{\circ}C$. In the case of animals acclimated to summer temperatures, the survival rates of adults and juveniles began to decrease from $35^{\circ}C$, and all died at temperatures between $40-44^{\circ}C$. The upper $LT_{50}$ was $27.72^{\circ}C$ for adults and $28.36^{\circ}C$ for juveniles. On the other hand, when the temperature was decreased from $4^{\circ}C\;to\;0^{\circ}C$ in order to investigate lower temperature tolerances, the survival rate of T. granosa was more than $70\%\;at\;2^{\circ}C$ for 25 days. Lower L T 50 was $2.09^{\circ}C$ for adults and $2.34^{\circ}C$ for juveniles. There was no effective difference in temperature tolerance between adults and juveniles. Filtration and respiration rates of T. granosa showed a similar aspect with increase and decrease of temperature. Filtration and respiration rates exhibited irregular conditions of a broken biological rhythm as the group acclimated to winter $(10 ^{\circ}C)$ and summer $(25^{\circ}C)$. In the case of decreasing temperature, filtration and respiration rates of T. granosa reduced to a minimum below $6^{\circ}C$

• Journal of Ecology and Environment
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• 제38권3호
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• pp.281-291
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• 2015

To clarify the effects of forest fire on the carbon budget of a forest ecosystem, this study compared the seasonal variation of soil respiration, net primary production and net ecosystem production (NEP) over the year in unburned and burned Pinus densiflora forest areas. The annual net carbon storage (i.e., NPP) was $5.75t\;C\;ha^{-1}$ in the unburned site and $2.14t\;C\;ha^{-1}$ in the burned site in 2012. The temperature sensitivity of soil respiration (i.e., $Q_{10}$ value) was higher in the unburned site than in the burned site. The annual soil respiration rate was estimated by the exponential regression equation with the soil temperatures continuously measured at the soil depth of 10 cm. The estimated annual soil respiration and heterotrophic respiration (HR) rates were 8.66 and $4.50t\;C\;ha^{-1}yr^{-1}$ in the unburned site and 4.08 and $2.12t\;C\;ha^{-1}yr^{-1}$ in the burned site, respectively. The estimated annual NEP in the unburned and burned forest areas was found to be 1.25 and $0.02t\;C\;ha^{-1}yr^{-1}$, respectively. Our results indicate that the differences of carbon budget and cycling between both study sites are considerably correlated with the losses of living plant biomass, insufficient nutrients and low organic materials in the forest soil due to severe damages caused by the forest fire. The burned Pinus densiflora forest area requires at least 50 years to attain the natural conditions of the forest ecosystem prior to the forest fire.

• 유기물자원화
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• 제18권2호
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• pp.84-92
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• 2010

본 연구는 강원도 T시를 중심으로 생활폐기물의 물리화학적 특성을 조사하여 생활폐기물의 적정관리 방안 및 처리방법에 활용할 수 있는 기초자료를 제공하고 하고자 실시되었다. 조사결과 T 시의 겉보기밀도는 평균 $231kg/m^3$이었으며, 가연성분과 비가연성분은 각각 94.0%와 6.0%이었다. 가연성분 중 음식물류폐기물(32.3%)과 종이류(41.2%)는 최근의 타 지역에 비하여 여전히 높은 비율을 차지하고 있어 철저한 분리배출이 필요하였다. 삼성분 중수분, 가연분, 회분의 함량은 각각 41.3%, 50.5%, 그리고 8.2%이었으며, 원소조성 중 탄소, 수소, 산소, 질소, 황, 염소의 함량은 각각 51.4%, 6.3%, 26.7%, 1.1%, 0.2%, 0.5%를 나타내었다. 습윤저위발열량은 2,704 kcal/kg으로 춘천시의 2,764 kcal/kg와 유사하였으나 과거 구로구, 고양시의 1,467~1,584 kcal/kg 보다는 1,000 kcal/kg 이상 높았다. 또한 조제된 소각재의 용출시험 실시결과 모두 지정폐기물 판정 기준치를 크게 하회하여 큰 문제가 없는 것으로 나타났다.

• Biomolecules & Therapeutics
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• 제15권3호
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• pp.187-191
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• 2007

This study was conducted to compare the bioavailability of two brands of atenolol (50 mg) tablets, which are a generic product of $Ditent^{\circledR}$ (Daewon Pharmaceutical Co., Ltd., Korea) and an innovator product $Tenormin^{\circledR}$ (Hyundai Pharm. Ind. Co., Ltd., Korea), in 20 healthy Korean male volunteers. The volunteers received a single 50 mg dose of each atenolol formulation according to a randomized, two-way cross-over design. The washout period between treatments was 1 week. Plasma samples were obtained over a 24-hour interval, and atenolol concentrations were determined by HPLC with a fluorescence detector. From the plasma atenolol concentration vs. time curves, the following parameters were compared: area under the plasma concentration-time curve ($AUC_{0-24}$), peak plasma concentration ($C_{max}$), time to reach peak plasma concentration ($T_{max}$), and terminal first order elimination half-life ($t_{1/2}$). No statistically significant difference was obtained between the $T_{max}$ values, and the logarithmic transformed $AUC_{0-24}$ and $C_{max}$ values of the two products. The 90% confidence interval for the ratio of the logarithmically transformed AUC and $C_{max}$ values of $Ditent^{\circledR}$ over those of $Tenormin^{\circledR}$ were calculated to be between 0.85 and 1.04, and 0.89 and 1.07, respectively; both were within the bioequivalence limit of 0.80-1.25. The mean of $T_{max}$ in $Tenormin^{\circledR}$ group was 3.1 hour, and that in Ditent$^{\circledR}$ group was 3.2 hour. The values of $t_{1/2}$ between the two products were found comparable, and the mean values were 5.2 hour in the both products. Based on these results, it was concluded that $Ditent^{\circledR}$ was comparable to $Tenormin^{\circledR}$ in both the rate and extent of absorption, indicating that $Ditent^{\circledR}$ was bioequivalent to the reference product, $Tenormin^{\circledR}$.

• Fisheries and Aquatic Sciences
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• 제20권9호
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• pp.20.1-20.8
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• 2017

The objective of this study was to examine the effect of dietary supplementation of taurine for juvenile olive flounder (Paralichthys olivaceus) at low water temperature ($16.4{\pm}0.36^{\circ}C$). Fish meal (FM)-based diet was used as the control diet. Four other experimental diets were prepared by adding taurine to FM-based diet at 0.25, 0.50, 1.00, and 1. 50% (T1, T2, T3, and T4, respectively). Each experimental diet was fed to triplicate groups of fish (initial mean body weight, 19.5 g) for 10 weeks. At the end of the feeding trial, growth performance and feed utilization, hematological parameters, non-specific immune responses, whole-body proximate composition, and liver mRNA expression of insulin-like growth factor-1 (IGF-1) were investigated. Feed conversion ratio was significantly reduced while protein efficiency ratio was significantly increased in taurine-supplemented groups. Hematocrit and hemoglobin were also significantly increased while plasma cholesterol levels were decreased in taurine-supplemented groups than those in the control group. Nitroblue-tetrazolium, myeloperoxidase and lysozyme activities, and plasma immunoglobulin level were significantly increased by taurine supplementation. These results suggest that dietary taurine supplementation is effective in improving growth performances, feed utilization, and innate immunity of olive flounder in low water temperature season.

• Korean Membrane Journal
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• 제10권1호
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• pp.26-32
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• 2008

The Gongji stream water of Chuncheon city was filtrated by 2 kinds of tubular alumina ceramic MF membranes with periodic $N_2$-back-flushing. $N_2$-back-flushing time (BT) was changed in $0{\sim}50$ sec at fixed filtration time (FT), or back-flushing period, of 4 min for NCMT-5231 membrane ($0.05\;{\mu}m$). Then, FT was changed in $0{\sim}32$ min at fixed BT of 40 sec for NCMT-7231 $0.1{\mu}m)$). In the viewpoints of total permeate volume ($V_T$), dimensionless permeate flux ($J/J_0$) and resistance of membrane fouling ($R_f$), the optimal $N_2$-BT was 50 sec, which was the longest BT, at 4 min FT for NCMT-5231. It means the longest BT was the most effective to minimize the membrane fouling, and we could acquire the most $V_T$. But the optimal FT for NCMT-7231 was 16 min in the viewpoint of $V_T$, and was 8 min in the viewpoints of $J/J_0$ and $R_f$ at fixed BT of 40 sec. The rejection rates were excellent as $80.6{\sim}96.6\;%$ for turbidity, $35.2{\sim}58.4%$ for $NH_3$-N, $16.3{\sim}45.2%$ for T-P and $16.3{\sim}45.2%$ for $COD_{Mn}$. However, the rejection rate of T-N was very low as $2.7{\sim}13.4%$ and it of TDS below 6.1%.

• 천문학회보
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• 제38권1호
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• pp.63.1-63.1
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• 2013

To improve the forecast capability of geomagnetic storms, we consider the real time solar and near Earth conditions together, since the characteristics of CMEs can be modified during their transit from the Sun to the Earth, and the geomagnetic storms may be directly affected by not only solar events but also near Earth interplanetary conditions. Using 55 CME-Dst pairs associated with M- and X-class solar flares, which have clearly identifiable source regions during 1997 to 2003, we confirm that the peak values of negative magnetic field Bz and duskward electric field Ey prior to Dst minimum are strongly related with Dst index. We suggest the solar wind criteria (Bz<-5 nT or Ey>3 mV/m for t>2 hr) for moderate storm less than -50 nT by modifying the criteria for intense storms less than -100 nT proposed by Gonzalez and Tsurutani (GT, 1987). As the results, 90% (28/31) of the storms are correctly forecasted by our criteria. For 15 exceptional events that are incorrectly forecasted by only CME parameters, 12 cases (80%) can be properly forecasted by solar wind criteria. When we applying CME and solar wind conditions together, all geomagnetic storms (Dst<-50 nT) are correctly forecasted. Our results show that, the storm forecast capability of the 2~3 days advanced warning based on CME parameters can be improved by combining with the urgent warning based on the near Earth solar wind condition.

• 한국통신학회논문지
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• 제31권6A호
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• pp.627-633
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• 2006

MBC(meteor burst communications)는 그 전송로의 많은 장점에도 불구하고 도통율이 수%이하로 낮다는 큰 결점이 있다. 이를 위해 큰 경제적 부담 없이 전송 채널의 도통율을 향상시키는 방법으로서 간단한 구조의 DS(Direct Sequence)시스템을 제안하였다. 제안된 DS 간이 수신방식은 동기신호의 포착이나 유지에 소요되는 시스템을 생략할 수가 있어서 MBC가 가지는 경제성을 만족시킬 수 있으나, 잡음의 영향을 크게 받아 도통율이 떨어지는 단점이 있다. 이를 위해 제안된 DS 시스템에 Transversal Filter회로를 적용하여 SNR을 6dB이상 향상시켰다. 이 방법으로 개선되는 효과는 수신신호가 약한 환경에서 보다 더 큰 것으로 나타나는데 시뮬레이션에 의하면 SNR이 약 6dB 개선되었을 때 평균 버스트 길이가 100ms인 것은 70%로 개선되어 170ms로 되고, 50ms환경에서는 130%개선되어 115ms가 되어 수신신호가 약한 환경에서 도통율이 크게 개선됨을 확인할 수 있었다.