• Parasites, Hosts and Diseases
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• 제50권2호
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• pp.157-159
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• 2012

The aim of this study was to assess the usefulness of PCR for diagnosis of Trichomonas vaginalis infection among male patients with chronic recurrent prostatitis and urethritis. Between June 2001 and December 2003, a total of 33 patients visited the Department of Urology, Hanyang University Guri Hospital and were examined for T. vaginalis infection by PCR and culture in TYM medium. For the PCR, we used primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Voided bladder urine (VB1 and VB3) was sampled from 33 men with symptoms of lower urinary tract infection (urethral charge, residual urine sensation, and frequency). Culture failed to detect any T. vaginalis infection whereas PCR identified 7 cases of trichomoniasis (21.2%). Five of the 7 cases had been diagnosed with prostatitis and 2 with urethritis. PCR for the 5 prostatitis cases yielded a positive 330 bp band from bothVB1 and VB3, whereas positive results were only obtained from VB1 for the 2 urethritis patients. We showed that the PCR method could detect T. vaginalis when there was only 1 T. vaginalis cell per PCR mixture. Our results strongly support the usefulness of PCR on urine samples for detecting T. vaginalis in chronic prostatitis and urethritis patients.

• Parasites, Hosts and Diseases
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• 제54권6호
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• pp.751-758
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• 2016

This study aimed at constructing a draft genome of the adult female worm Toxocara canis using next-generation sequencing (NGS) and de novo assembly, as well as to find new genes after annotation using functional genomics tools. Using an NGS machine, we produced DNA read data of T. canis. The de novo assembly of the read data was performed using SOAPdenovo. RNA read data were assembled using Trinity. Structural annotation, homology search, functional annotation, classification of protein domains, and KEGG pathway analysis were carried out. Besides them, recently developed tools such as MAKER, PASA, Evidence Modeler, and Blast2GO were used. The scaffold DNA was obtained, the N50 was 108,950 bp, and the overall length was 341,776,187 bp. The N50 of the transcriptome was 940 bp, and its length was 53,046,952 bp. The GC content of the entire genome was 39.3%. The total number of genes was 20,178, and the total number of protein sequences was 22,358. Of the 22,358 protein sequences, 4,992 were newly observed in T. canis. Following proteins previously unknown were found: E3 ubiquitin-protein ligase cbl-b and antigen T-cell receptor, zeta chain for T-cell and B-cell regulation; endoprotease bli-4 for cuticle metabolism; mucin 12Ea and polymorphic mucin variant C6/1/40r2.1 for mucin production; tropomodulin-family protein and ryanodine receptor calcium release channels for muscle movement. We were able to find new hypothetical polypeptides sequences unique to T. canis, and the findings of this study are capable of serving as a basis for extending our biological understanding of T. canis.

• 한국콘텐츠학회논문지
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• 제20권2호
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• pp.472-477
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• 2020

본 연구는 자기공명영상 검사 시 전자파흡수율을 감소시키는 대안으로 환자정보의 체중을 낮게 입력하는 방법의 오류를 바로잡고자 하였다. 연구방법은 환자정보에 입력하는 체중에 따라 전자파흡수율이 변화되지 않는다는 사실을 증명하기 위해 50kg 팬텀을 코일 안에 위치시킨 후 입력 체중을 10kg에서부터 100kg 까지 10kg 단위로 변화시켜 기기에서 산출되는 전자파흡수율을 비교평가 하였다. 연구결과, T1 강조영상은 전자파 흡수율이 평균 0.2W/kg이었고 T2 강조영상은 평균 0.4W/kg이었으나, T1, T2 강조영상 모두 체중의 변화에 따른 전자파흡수율의 변화는 없었다. 결론적으로 검사 전 검사자가 입력하는 체중에 따라 전자파흡수율은 변화되지 않으므로, 환자정보 입력 시 체중을 낮게 입력하는 것은 전자파흡수율을 감소시키는 대안이 될 수 없다.

• 한국대기환경학회지
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• 제32권1호
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• pp.9-20
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• 2016

Ozone has been a problem in big cities. That is secondary air pollutant produced by nitrogen oxide and VOCs in the atmosphere. In order to solve this, the first to be the analysis of the $NO_x$ and VOCs. The main source of nitrogen oxide is the road mobile. Industrial sources in Seoul are particularly low, and mobile traffics on roads are large, so 45% of total $NO_x$ are estimated that road mobile emissions in Seoul. Thus, it is necessary to clarify the relation with the activity of road mobile source and $NO_x$ concentration. In this study, we analyzed the 4 locations with roadside automatic monitoring systems in their center. The V.K.T. calculating areas are set in circles with 50 meter spacing, 50 meter to 500 meter from their center. We assumed the total V.K.T. in the set radius affect the $NO_x$ concentration in the center. We used the hourly $NO_x$ concentrations data for the 4 observation points in July for the interference of the other sources are minimized. We used the intersection traffic survey data of all direction for construction of the V.K.T. data, the mobile activities on the roads. ArcGIS application was used for calculating the length of roads in the set radius. The V.K.T. data are multiplied by segment traffic volume and length of roads. As a result, the $NO_x$ concentration can be expressed as linear function formula for V.K.T. with high predictive power. Moreover we separated background concentration and concentrations due to road mobile source. These results can be used for forecasting the effect of traffic demand management plan.

• 대한치과교정학회지
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• 제50권2호
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• pp.120-128
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• 2020

Objective: The aims of the present study were to evaluate the changes in the maximum lip-closing force (MLF) after orthodontic treatment with or without premolar extractions and verify the correlation of these changes with dentoskeletal changes. Methods: In total, 17 women who underwent nonextraction orthodontic treatment and 15 women who underwent orthodontic treatment with extraction of all four first premolars were included in this retrospective study. For all patients, lateral cephalograms and dental models were measured before (T0) and after (T1) treatment. In addition, MLF was measured at both time points using the Lip De Cum LDC-110R^® device. Statistical analyses were performed to evaluate changes in clinical variables and MLF and their correlations. Results: Both groups showed similar skeletal patterns, although the extraction group showed greater proclination of the maxillary and mandibular incisors and lip protrusion compared to the nonextraction group at T0. MLF at T0 was comparable between the two groups. The reduction in the arch width and depth and incisor retroclination from T0 to T1 were more pronounced in the extraction group than in the nonextraction group. MLF in the extraction group significantly increased during the treatment period, and this increase was significantly greater than that in the nonextraction group. The increase in MLF was found to be correlated with the increase in the interincisal angle and decrease in the intermolar width, arch depth, and incisor-mandibular plane angle. Conclusions: This study suggests that MLF increases to a greater extent during extraction orthodontic treatment than during nonextraction orthodontic treatment.

• 한국산학기술학회논문지
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• 제14권6호
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• pp.2880-2888
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• 2013

본 연구는 듀라터치 분만교육이 분만자신감, 분만지식, 부부관계 만족도에 미치는 효과를 규명하고자 시도되었다. 대상자는 경기도 Y시에 소재한 보건소에서 2012년 10월부터 11월까지 주 1회 4번 듀라터치 분만교육을 받은 임산부 27명과 경기도 W시에 위치한 D병원에서 2012년 10월부터 2012년 11월 산전 진찰을 받은 대조군 28명을 대상으로 연구했는데, 그 결과는 다음과 같다. 듀라터치 교육을 받은 실험군이 대조군보다 분만자신감(t=-2.81, p=.007)과 분만지식정도(t=3.50, p=.001)에서 통계적으로 유의미하게 높았으나 듀라터치 교육을 받은 실험군과 대조군 간에는 부부관계 만족도는 유의미한 차이가 없는 것으로 나타났다(t=0.23, p=.818). 분만자신감과 부부관계 만족도에서 통계적으로 높은 상관관계를 나타냈다(r=314, p=.005).

• 한국약용작물학회지
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• 제23권3호
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• pp.245-252
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• 2015

This study was carried out to investigate the effects of Salvia plebeia R. Br. ethanolic extract with different aspects (stem/leaf and whole plant) on differentiation and lipid accumulation in 3T3-L1 preadipocytes. The morphological changes and the degrees of lipid accumulation in 3T3-L1 cells were measured by Oil Red O staining and intra-cellular triglyceride (TG) assay. The mRNA expressions of special peroxisome proliferation activated receptor- genes (PPAR), CCAAT/ enhancer-binding protein (C/$EBP{\alpha}$), fatty acid synthase (FAS) and lipoprotein lipase (LPL) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). The 50% ethanolic extracts ($100{\mu}g/mL$) of stem and leaf (SALE) and 30% ethanolic extracts (100 g/mL) of whole plant (SAE) from Salvia plebeia R. Br. were significantly attenuated lipid accumulation during adipogenesis in 3T3-L1 cells. Ethyl acetate-soluble fractions ($50{\mu}g/mL$) significantly inhibited lipid droplet accumulation in 3T3-L1 cells. In addition, SALE induced down-regulation of specific adipogenic transcriptional factors (C/$EBP{\alpha}$ and $PPAR{\gamma}$) and target genes (FAS and LPL) during adipogenesis. Salvia plebeia R. Br. may be used as a safe and efficient natural substance to manage obesity.

• 한국보건간호학회지
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• 제16권1호
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• pp.135-147
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• 2002

It is essential to improve the quality of life of the elderly in aging society. Regular exercise as nursing intervention is needed to prevent diseases, maintain and promote their health, and thus promote the quality of life for the elderly, especially who generally had poor cognition for their health maintenance and promotion in a low class community. Subjects consisted of 30 women. over 65 years old in a community welfare center, who lived in the low class apartments financially supported by the local government in G city, Gangwon Province, Korea. The program consisted of dancing on music for approximately 50 minutes, 3 times a week for 10 weeks. Experimental data were analyzed with $x^2-test$, t-test, mean values, standard deviation, percentage of change and paired t-test using SAS program. After the recreation dance, the mean value of the experimental group was decreased from 7.62 to 7.44 (t=-0.258, P=0.799), and that of the control group was also decreased from 7.00 to 6.58 (t=-0.971, P=0.349) in depression. However, there were no statistically significant differences in scores of depression between two groups. In life satisfaction, the mean value of the experimental group was increased from 19.07 to 26.50 (t=2.392, P=0.030), but that of the control group was decreased from 15.92 to 13.71 (t=-1.060, P=0.305). Thus it shows that there was a statistically significant improvement on life satisfaction after the recreation dance.

• 한국전자파학회논문지
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• 제11권5호
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• pp.782-787
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• 2000

광대역 고출력 SP3T MMIC GaAs PIN 다이오드 스위치를 설계, 제작하고 특성을 측정하였다. 전력단속능력을 개선시키기 위하여 다이오드의 버퍼층을 저온 버퍼와 초격자 버퍼로 이루어진 2층 구조로 설계하였다. 개발된 다이오드의 항복전압은 65V이고 순방향 정압강하는 1.3V 이었다. MMIC 스위치는 마이크로스트립 라인형으로 구현되었고 인덕턴스가 낮은 via hole 공정을 이용하여 신호를 접지하였다. 평면형 구조보다 더 낮은 기생성분과 진성영역에서 고품질을 갖는 수직형 에피텍셜 PIN 구조를 사용하여 우수한 마이크로파 성능을 얻었다. 제작된 SP3T 스위치의 고출력 특성은 14.5GHz CW에서 입력전력을 8dBm부터 32dBM 까지 증가시킬 때 삽입손실은 0.6dB보다 작은, 분리도는 50dB보다 크게 측정되었다.

• 한국정보통신학회논문지
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• 제20권2호
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• pp.351-358
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• 2016

경량 블록 암호 알고리즘 CLEFIA의 효율적인 하드웨어 설계에 대하여 기술한다. 설계된 CLEFIA 보안 프로세서는 128/192/256-비트의 세 가지 마스터키 길이를 지원하며, 변형된 GFN(Generalized Feistel Network) 구조를 기반으로 8-비트 데이터 패스로 구현되었다. 라운드키 생성을 위한 중간키 계산용 GFN과 암호 복호 라운드 변환용 GFN을 단일 데이터 프로세싱 블록으로 구현하여 하드웨어 복잡도를 최소화하였다. 본 논문의 GFN 블록은 라운드 변환과 128-비트의 중간 라운드키 계산을 위한 4-브랜치 GFN과 256-비트의 중간 라운드키 계산을 위한 8-브랜치 GFN으로 재구성되어 동작하도록 설계되었다. Verilog HDL로 설계된 CLEFIA 보안 프로세서를 FPGA로 구현하여 정상 동작함을 확인하였다. Vertex5 XC5VSX50T FPGA에서 최대 112 MHz 클록으로 동작 가능하며, 마스터키 길이에 따라 81.5 ~ 60 Mbps의 성능을 갖는 것으로 평가되었다.