• Title/Summary/Keyword: $T_{1/2}$

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The Detection of T-2 toxin in Serum and Organ of Mouse by ELISA (ELISA법에 의한 mouse의 혈청 및 조직중의 T-2 toxin의 검색)

  • 김동술;송재영;정덕화
    • Journal of Environmental Health Sciences
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    • v.22 no.1
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    • pp.51-56
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    • 1996
  • In order to detect the T-2 toxin accumulation in the animal tissues, T-2 toxin, produced by Fusarium sporotrichioides M-1-1, was injected to mouse by 0, 1 and 2 mg per kilogram of body weight, respectively, and T-2 toxin extracted from serum and organs were analyzed by the indirected competitive ELISA. The indirect competitive ELISA established in the laboratory can be check less than 0.1 ppb level of T-2 toxin and average recovery of T-2 toxin spiked was 80~113% in animal samples such as serum, liver and kidney. After 6 weeks of treatment with 2 mg of T-2 toxin per kg body weight, T-2 toxin was accumulated in serum (133.0 ng/ml), liver(1.4 ng/g) and kidney(14.3 ng/g) of mouse injected with 2 mg of toxin per kg body weight.

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Comparison of Single and Double Combination of Temperature-time in Sous Vide Treated Semitendinosus Muscle from Cattle and Goat

  • Ismail, Ishamri;Hwang, Young-Hwa;Joo, Seon-Tea
    • Food Science of Animal Resources
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    • v.39 no.1
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    • pp.45-53
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    • 2019
  • This study observed the effects of the double combination of temperatures-times (2 temperatures: 2 times combination, 2T2T) in sous vide cooking method on the physicochemical properties and collagen solubility (CS) of semitendinosus muscle from cattle and goat as a comparison to common sous vide treatment (1 temperature: 1 time combination, 1T1T). The new invention of sous vide cooking method (2T2T) cooked at the first temperature at $45^{\circ}C$ for 3 h, and the second temperature at $60^{\circ}C$, $65^{\circ}C$, and $70^{\circ}C$ for 3 h, and labeled as N45-60, N45-65, and N45-70, respectively. While, common sous vide treatment (1T1T) were cooked directly for 6 h at $60^{\circ}C$ (T60), $65^{\circ}C$ (T65), and $70^{\circ}C$ (T70). Results revealed that cooking with 2T2T treatment improved the water-holding capacity and reduced the cooking loss of both beef and goat meat. The $L^*$ values have no apparent changes between treatment in beef and goat meat, while $a^*$ values of N45-60 treated goat presented markedly higher values than other treatments but an only slight increase in beef at the same treatment (p>0.05). Again, 2T2T treatment tended to decrease mean shear force (SF) values for beef and goat meat with the lowest SF values recorded at N45-60, and the CS no or less influenced this value. Therefore, the application of innovative sous vides cooking method (2T2T) presented comparable values in the treated beef and goat meat as compared to common sous vide method (1T1T).

Physico-Chemical, Fatty Acid Composition and Sensory Properties of Venison from Cervus elaphus andadensis (Elk Deer) (엘크 사슴육의 물리화학적, 지방산 조성 및 관능적 품질 특성)

  • Kim Il-Suk;Jin Sang-Keun;Hah Kyung-Hee;Park Seok-Tae;Kwuak Kyung-Rak;Park Jung-Kwon;Kang Yang-Su
    • Food Science of Animal Resources
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    • v.26 no.1
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    • pp.70-77
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    • 2006
  • This study was carried out to evaluate the quality characteristics on the two different muscles (Loin; T1, Ham; T2) from the Cervus elaphus andadensis (Elk deer). The water content and shear force of T1 were lower than that of T2. The protein content and water holding capacity were lower in T2 compared to the T1. In meat color, $L^*,\;a^*\;and\;b^*$ values were not different between T1 and T2. In the texture properties, there were significantly (p<0.05) differences in hardness, adhesiveness, gumminess and brittleness, but cohesiveness and springiness were not different significantly (p<0.05). In fatty acid analysis, myristic acid and linoleic acid of T2 were high (p<0.05) as 5.06 and 10.37% respectively, while palmitoleic acid of T1 were significantly (p<0.05) higher than that of T2. SFA and UFA were not different between the T1 and T2, although EFA of T1 was significantly (p<0.05) higher than that of T2. In sensory evaluation, acceptability of T2 in fresh meat showed the slightly high, but that of T2 in cooked meat was slightly low score. All samples were not significantly different in overall acceptability.

Effects of Agricultural By-Product Feeds on Growth and Carcass Characteristics of Korean Native Steer (농산 혼합 부산물 첨가급여가 송아지 질병 및 거세 한우 성장 및 도체특성에 미치는 영향)

  • Lee, Sang-Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.28 no.1
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    • pp.41-48
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    • 2008
  • This study was carried out investigate the effects of dietary supplementation of agricultural by-product feed(persimmon-pell+barley bran+fermented rice bran+activated carbon) on calves disease, growth and carcass characteristics of Korean native steer. A total of twenty Korean native steer were allocated into four feeding group and assigned to four dietary treatments: Control(normal concentrate as a basal diet), T1(1% addition of by-product feeds), T2(3% addition of by-product feeds) and T3(5% addition of by-product feeds). The calf diarrhea was the highest at Control, while T3 was the lowest. The breath disease was the lowest at T2, occurrence with diarrhea and breath disease was the highest at Control. The daily gain was orderly ranked as T2(0.76kg)>T1(0.7kg), T3(0.70kg)>Control(0.67kg). The back fat thickness was orderly ranked as T2>T3>Control>T1, eye muscle area was orderly ranked as T3>T1>Control>T2. Grade of meat quantity was the highest at T1(2.3), but fat color, firmness and maturity tends to have a similar result. The marbling score and quality grade of T2 were the highest at 5.0 and 3.5, respectively. Based on the study, agricultural by-product feeds are able to many used for various purposes at disease reduce, increment of meat quantity and improvement of quality grade.

Effects of Dietary Fish Oil, Vitamin E and C Supplementation on DHA Deposition and Shelf-Life in Broiler Chickens (어유, 비타민 E 및 C의 급여가 닭고기의 DHA 축적 및 저장성에 미치는 영향)

  • Kang, H.K.;Kim, S.H.;Kim, J.H.;Kang, G.H.;Yu, D.J.;Na, J.C.;Kim, D.W.;Seo, O.S.;Kim, G.H.;Park, B.S.
    • Korean Journal of Poultry Science
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    • v.34 no.4
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    • pp.259-269
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    • 2007
  • This study was compared the effect of shelf-life and DHA accumulation in chicken meat from broilers fed experimental diets for two weeks($21{\sim}35$ days) of growers. Two hundred-ten male Ross broilers, 1 day of age, were randomly allocated to seven treatment groups. Experimental diets were assigned to each of the seven groups: control diet containing tallow, T1 with 1.00% fish oil, T2 with 2.00% fish oil, T3 with 2.00% fish oil, 200 ppm vitamin E and 200 ppm vitamin C, T4 with 2.00% fish oil and 200 ppm vitamin C, T5 with 2.00% fish oil and 200 ppm vitamin E, and T6 with 3.00% fish oil. The levels of DHA in chicken meat was the highest in T6, and T1 in breast muscle and thigh muscle with skin was higher than that of $T2{\sim}T5,\;T2{\sim}T5$ in raw chicken meat and wing with skin was about two-fold higher than that of T1. The contents of DHA in chicken meat according to storage days were significantly reduced to 42.30%, 49.38% and 48.51% in T1, T2 and T6, respectively, and this decrease was higher than that of T3, T4 and T5 (p<0.05). Particularly, the rate of reduction of DHA was the lowest in the T3 and T5, which were the lowest in TBARS(thiobarbituric acid reactive substances). TBARS increased in the order of T6, T2 and T1, but reduced in the order of T3, T5 and T4 according to storage days, and there was a significant difference among the treatment groups (p<0.05).

Effect of Dietary Xanthophylls Supplementation on Pigmentation and Antioxidant Properties in the Egg Yolks (Xanthophylls의 급여가 계란 노른자의 착색 및 항산화성에 미치는 영향)

  • Min, B.J.;Lee, K.H.;Lee, S.K.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.847-856
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    • 2003
  • This study was carried out to investigate the effects of dietary xanthophylls supplementation on pigmentation and antioxidant properties in the egg yolk. ISA Brown laying hens aged 50 weeks were fed five kinds of xanthophyll combination such as control(neither natural nor artificial xanthophylls in feed), T1(Commercial diet containing natural xanthopylls in grain + Lutein 10ppm + Capsantin 10ppm), T2(T1 + Capsanthin 65ppm), T3(T1 + Canthaxanthin 65ppm), T4(T1 + Capsanthin 10ppm + Canthaxanthin 65ppm), and T5(T1 + Capsanthin 65ppm + Canthaxanthin 10ppm). The pH values of all egg yolks were not significantly different during storage or feeding periods. CIE L$^{*}$(lightness) values of T2 ~ T5 egg yolks were lower than those of control and T1. Conversely, the CIE a$^{*}$(redness) value of T2 ~ T5 egg yolks showed significantly higher(P<0.05). Egg yolk from chicks fed xanthophylls increased CIE b$^{*}$ values. The CIE b$^{*}$(yellowness) values of T2 ~ T5 egg yolks were higher than control and T1 during storage at 37$^{\circ}C$ for 48 hours respectively. In the antioxidation experiment, dietary xanthophylls supplementation affected lipid antioxidation of egg yolk during storage. The TBARS(O.D.) of egg yolks from chicks fed xanthophylls were lower than that of control during incubation at 37$^{\circ}C$ for 10 hours. In conclusion, dietary xanthophylls supplementation influence to color difference and retardation of lipid oxidation in egg yolk.

동시획득 T1/T2 강조 경사자장 펄스열을 이용한 근골격계에서의 종양 관류 영상: 예비보고

  • 허용민;서진석;김대용;김은주
    • Proceedings of the KSMRM Conference
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    • 2002.11a
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    • pp.137-137
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    • 2002
  • 목적: 동시획득 T1/T2 강조 경사자장 펄스열을 이용하여 근골격계의 종양 관류 평가를 하고자 한다. 대상 및 방법: 근골격계 양성 및 악성 종양을 대상으로 동시획득 T1/T2 강조 경사자장 펄스열을 이용하여 시간해상도를 1.2초로 하여 1000회(약20분)를 반복하여 역동적 영상을 얻는다. 각각의 TR/TE1/TE2는 10/2/8 msec이다. 각 시기에서 서로 다른 TE를 가지고 있는 두 개의 영상을 이용하여, 수학적으로 분리하여 T1과 T2 값을 얻고, 이를 시간에 따라 배열한다. 이를 통하여, T2의 경우에는 일차효과를 이용하여 조직관류량(tissue blood volume)을 측정하고, T1에서는 2구획모델을 이용하여 투과도(permeability)를 측정한다.

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Analyzing the Significance of T1 Slope minus Cervical Lordosis in Patients with Anterior Cervical Discectomy and Fusion Surgery

  • Lee, Ho Jin;You, Soon Tae;Sung, Jae Hoon;Kim, Il Sup;Hong, Jae Taek
    • Journal of Korean Neurosurgical Society
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    • v.64 no.6
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    • pp.913-921
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    • 2021
  • Objective : Accurate measurement of T1 slope (a component of T1s minus cervical lordosis [CL]) is often constrained by anatomical limitations. In this situation, efforts should be made to find the exact meaning of T1s-CL and whether there are any alternatives to it. Methods : We enrolled 117 patients who received two-level anterior cervical discectomy and fusion (ACDF). Occipital slope, C2 slope (C2s), C7 slope (C7s), T1, O-C2 angle (O-C2A), C2-7 angle (C2-7A), O-C7 angle (O-C7A), T1s-CL, C7-T1 angle (C7-T1A), and C2-7 sagittal vertical axis were measured. We determined 16° (T1s-CL) as the reference point for dividing subjects into the mismatch group and the balance group, and a comparative analysis was performed. Results : The mean value of C7-T1A was constantly maintained within 2.6° peri-operatively. In addition, C2s and T1s-CL showed the same absolute change (Δ|0.8|°). The mean values of T1s-CL of the mismatch and balance groups were 23.0° and 7.6°, respectively. The five factors with the largest differences between the two groups were as follows : C2s (Δ13.3°), T1s-CL (Δ15.4°), O-C2A (Δ8.7°), C2-7A (Δ14.7°), and segmental angle (Δ7.9°) before surgery. Only four factors showed statistically significant change between the two groups after ACDF : T1s-CL (Δ4.0° vs. Δ0.2°), C2s (Δ3.2° vs. Δ0.7°), O-C2A (Δ2.6° vs. Δ1.3°), C2-7A (Δ6.3° vs. Δ1.3°). A very strong correlation between T1s-CL and C2s was also found (r=|0.88-0.96|). Conclusion : C2s itself may be the essential key to represent T1s-CL. The amounts and directions of change of these two factors (T1s-CL and C2s) were also almost identical. The above phenomenon was re-confirmed once again through the correlation analysis.

A Study on the Environmental Characteristics of a Two-storey Pig House for the Control of Manure (고상식돈사의 분뇨처리에 관한 연구)

  • Jeong, J.W.;Yoo, Y.H.;Park, K.H.;Kam, D.H.;Lee, M.J.
    • Journal of Animal Environmental Science
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    • v.14 no.3
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    • pp.175-182
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    • 2008
  • This research was conducted to examine proper manure treatment methods for a high-rise swine barn with results of the fact-finding survey on manure treatment methods used in high-rise swine barns and sawdust swine barns In Korea. The average temperatures on bedding of control, T1, and T2 were between $25.3{\sim}25.4^{\circ}C$ with little difference. Air flow rate of T2 was slower than those of control and T1. Dust generation in control was 54 cpm which was higher than T1 and T2. The average ammonia concentration in control was highest showing 6.0 ppm, ranged from 0.5 to 14.5 ppm, with statistical difference (p<0.05), compared to T1 (2.8 ppm) and T2 (2.6 ppm). Sawdust spreading used in control and T1 decreased ammonia concentration. T1 showed the lowest concentration of $CO_2$. Water contents of control and T2 were close to the proper water content, 65%. T1 had the highest water content and pH, and the lowest organic matter and C/N ratio. The volumes of sawdust spread per head were 0.26 and $0.27m^3$ in control and T2, respectively, which was less than T1. Operating cost such as an electricity bill for blowers was cheap in T2. Hence, T2 bedding sawdust up to 10 cm thick and periodically spreading additional sawdust from second story was recommended.

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Optimum Harvest Stage of Italian Ryegrass 'Kowinearly' According to One and Two Harvests During Spring Season (이탈리안 라이그라스 '코윈어리'의 봄철 1회 및 2회 이용에 따른 수확적기 구명)

  • Seo, Sung;Kim, Meing Jooung;Kim, Won Ho;Lee, Sang Hak;Jung, Min Woong;Kim, Ki Yong;Ji, Hee Chung;Park, Hyung Soo;Kim, Jong Geun;Choi, Gi Jun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.1
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    • pp.15-20
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    • 2013
  • This study was carried out to determine the optimum harvest stage of Italian ryegrass (Lolium multiflorum Lam., IRG) for maximum forage production during the spring season in Suwon, 2010. The variety of IRG was the early maturity type, 'Kowinearly', and six harvest stages (treatments) were first heading (T1), heading (T2), late heading to early bloom (T3), bloom to late bloom (T4), ripeness (T5), and late ripeness stage (T6). The dates of the first heading and heading of 'Kowinearly' were seen on 4 to 5 May, and 14 May, respectively. Plant length and dry matter (DM) percentage at first harvest were from 69 cm and 14.8% at T1 stage to 103 cm and 35.0% at T6 stage, respectively. The content of crude protein (CP) and in vitro DM digestibility (IVDMD) of T1, T2, T3, T4, T5 and T6 at first harvest were 15.6%, 10.6%, 10.1%, 8.1%, 7.3% and 5.4%, and 81.8%, 72.1%, 64.8%, 63.8%, 61.4% and 59.0%, respectively. The content of neural detergent fiber (NDF) and acid detergent fiber (ADF) were increased continuously with delayed harvest. A significantly higher yield of DM, CP and in vitro digestible DM (IVDDM) were observed for T3, and T4 (p<0.05). DM yield of 3,526 kg, 6,278 kg, 7,842 kg, 8,984 kg, 8,346 kg and 8,008 kg/ha, CP yield of 549 kg, 665 kg, 795 kg, 725 kg, 608 kg and 430 kg/ha, and IVDDM of 2,883 kg. 4,526 kg, 5,083 kg, 5,728 kg, 5,124 kg and 4,722 kg/ha at first harvest were recorded in T1, T2, T3, T4, T5 and T6, respectively. Regrowth yield of DM, CP and IVDDM were shown to be higher at T1 and T2 (p<0.05). However, no significant differences were observed between the two stages. Daily DM and DDM production of regrowth IRG were higher at T2, followed by T1. The total yield (at first and at regrowth) of DM, CP and IVDDM were significant higher for T2, followed by T3, T4 and T1 in order. At T2 stage, the yield was 11,089 kg, 1,254 kg, and 7,669 kg/ha in DM, CP, and IVDDM. In conclusion, the late heading to bloom stage was determined to be the optimum harvest stage for a single harvest, while the heading stage was a suitable stage of first harvest of 'Kowinearly' where two harvests were sought in a single year.