• 한국작물학회지
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• 제36권3호
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• pp.266-270
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• 1991

미곡의 장기보관에서 보관면적과 소요비용을 절약하려면 정조보관보다 현미 또는 백미로 보관하는 것이 용이하다. 그러나 미곡의 고미화와 식미 악화가 우려되어 본 연구에서는 20개월간 정부창고에서 보관된 벼품종들을 정조, 현미, 백미로 가공하고 각각 지대, PP대, PE대에 포장하여 수확 후 32개월째에 발아력, 지방산도, 식미를 검정하였다. 1. 발아율은 장기 보관함에 따라 감소하였고 밀양 2003보다 추청의 감소가 심하였다. 가공상태에 따라 현미로 제현하여 보관하면 발아율 감소가 현저하였고 포장재질에 따른 차이는 없었다. TTC검정도 유사한 경향을 보여 발아력의 간접검정법으로 이용될 수 있었다. 2. 지방산도는 정조<백미<현미 순으로 높아졌고 포장재료에 따라서는 PP대<지대<PE대 순으로 증가되었다. 특히 현미 보관은 지방산도가 KOH 30mg 이상에 달하여 고미화 특성을 보였고 이를 방지하려면 정조를 지대 또는 PP대에 보관하거나 백미는 지대, PP대, PE대에 보관하여 도 무난하였다. 3. 저장미곡의 식미는 도정보관에 따라 악화되었고 특히 현미보관에서 백미보관 보다 불량하였는데 호분층에 포함된 지방의 산패 때문이었다. 또 품종별로 지방산도가 증가함에 따라 식미는 불량하여 졌다.

• 대한지리학회지
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• 제40권1호
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• pp.96-108
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• 2005

그동안 꾸준히 강조되어 온 지리적 사고력은 오늘날 고차적 사고력의 함양으로 특히 강조되고 있다. 고차적 사고력으로서의 지리적 사고력의 함양은 교수-학습 과정이 사고기능을 포함한 지리적 기능 중심의 학습자 활동으로 구체화될 때 실현될 수 있지만, 우리나라 지리교육에서는 이에 대한 논의가 거의 이루어지지 못하고 있다. 이에 본 연구는 최근 영국의 국가교육과정에서 제시하는 사고기능과 그것을 지리교육과정으로 구체화한 '지리를 통한 사고 ' 전략에 대해서 살펴보았다. 하나의 사고력 교수 방법으로서 제시되는 TTG는, 무엇보다도 다양한 차이를 가지고 있는 학습자의 학습을 장려하기 위한 구체적이고도 실제적인 교수-학습 전략이다. 따라서 본 연구는 우리나라 지리교육에서 학습자의 사고력을 함양하기 위한 교수-학습 내용과 방법을 모색하는데 필요한 이론적, 실제적 통찰을 제공할 것이다.

• 한국임상약학회지
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• 제7권1호
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• pp.33-39
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• 1997

Cardiac and antihypertensive effects of BMS-180448, a cardiac-selective ATP-sensitive potassium channel opener, and its newly synthesized derivatives KR-31281, KR-31282 and KR-31299 were evaluated in isolated perfused rat hearts (25 min global ischemia/30 min reperfusion) and conscious rats. Three new compounds $(10\;{\mu}M)$ induced positive inotropism as evidenced by increased LVDP (left ventricular developed pressure) and RPP (Rate-Pressure Product) in nonischemic rat heart. HR-31299 increased CF (coronary flow) and HR (heart rate) but the other two had no effects. KR-31282, KR-31281 and HR-31299 had a tendency to increase reperfusion LVDP and RPP compared with vehicle, while the latter two significantly reduced reperfusion EDP with a tendency to inclose TTC (time to contracture). All three KR-compounds had very weak effects on MBP and HR in conscious rats. These results indicate that KR-31281 and HR-31299 may have some cardioprotective effects, although weaker than BMS-180448, and their mode of action different from that of BMS-180448, despite the similarity in major structural moeity.

• BMB Reports
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• 제30권1호
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• pp.21-25
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• 1997

The growth. freezing resistance and electrophoretic protein patterns of carrot callus cultures were investigated following treatment with NaCl for various' intervals at 20$^{\circ}C$. Following 7 day exposure to 250 mM NaCl. freezing tolerance increased, which was measured by 2.3.5-triphenyl tetrazolium chloride (TTC) assay and fresh weight was reduced compared to control cells. Changes of electrophoretic patterns of total and boiling stable proteins were investigated using one or two dimensional gel system. Several proteins with molecular weight of 43 and 21 kDa increased by NaCl treatment. The most prominent change was detected in 21 kDa protein. The steady state level of this protein increased in NaCl treated cells, but decreased in control cells. Twenty one kDa protein was detected only in the NaCl treated cell when boiling stable protein was analyzed. The isoelectric point of 21 kDa protein was identified as 5.7. The timing of increase of 21 kDa protein was correlated to freezing resistance which implied the role of this protein in the induction of freezing resistance of the cell.

• The Plant Pathology Journal
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• 제22권4호
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• pp.369-374
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• 2006

To characterize benzimidazole-resistant and -sensitive Monilinia fructicola populations, the fungal isolates were obtained from peach plants showing brown rot and bloosom blight. Benzimidazole-sensitive isolates did not grow on potato dextrose agar(PDA) amended with $\geq1.0{\mu}g$ active ingredient(a.i.)/ml of the fungicides. However, benzimidazole-resistant isolates grew on PDA regardless of the tested concentrations of fungicides. Benzimidazole-resistant isolates did not grow on diethofencarb-PDA, but sensitive isolates grew on the same PDA. In the nucleotide sequences of $\beta$-tubulin gene, only codon 198(GAG: glutamic acid), a target site for benzimidazole, was replaced with GCG(alanine) in all of the resistant isolates, and this substitution seems to play an important role in the development of resistance. Other interesting codons such as 165(GCT), 200(TTC), and 241(GCT) were not changed among the isolates. Benzimidazole-resistant and -sensitive isolates were clustered clearly in random amplified polymerphic DNA analyses and the results revealed that low levels of genetic diversity between benzimidazole-sensitive and -resistant isolates of M. fructicola in the investigated regions.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.65-68
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• 2009

본 연구에서는 eagle 2000 glass위에 APCVD(atmospheric CVD)증착법으로 $SnO_2$:F 박막을 제조하였다. 공정 온도, doping 농도, TTC(Tin tetrachloride)와 $H_2O$, $CH_3OH$의 조성비를 공정 변수로 두었으며, 각 변수에 대한 전기적, 광학적 특성 및 결정성을 확인하였다. hall measurement를 이용 제작된 박막의 전기적 특성을 확인 하였고, uv-VIS spectroscopy, hazemeer를 이용 박막의 광학적 특성을 확인 하였다. 또한 XRD, FESEM, AFM을 이용 박막의 결정성 및 표면 특성을 확인 하였다. 박막의 결정성을 결정짓는 증착 온도의 경우 $590^{\circ}C$에서 완벽한 Tetragonal rutile 형태의 결정성을 보였으며 $SnO_2$:F film $1{\mu}m$ thickness에서 $10({\Omega}/{\square})$ 내외의 우수한 면저항값과 $30(cm^2/Vs)$ 이상의 mobility값을 확인 하였으며, 가시광영역대 에서 높은 투과율과 우수한 haze값을 얻었다.

• 대한한의학회지
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• 제30권1호
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• pp.64-75
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• 2009

Objective: This study was designed to investigate the effect of Samultang (SMT) under hippocampus cells ischemia both in vitro and in vivo. Methods: In the in vitro study, HT22 cells, predominantly detected in the cytoplasm, which coincides with the location of the mitochondria, were used as indicators. In the in vivo study, permanent middle cerebral artery occlusion (MCAO) was induced on rats. SMT was given orally 2 h before induction of permanent focal brain ischemic injury. Result: In the in vitro study, SMT had protective effects in glutamate-induced cytotoxicity, which was revealed as apoptosis characterized by chromatic condensation and the loss of mitochondrial membrane potential in HT22 cells. In the in vivo study, TTC (2,3,5-triphenyltetrazolium chloride) staining showed a marked ischemic injury in blood supply territory of the middle cerebral artery (MCA) such as the cerebral cortex and striatum. However, treatment with SMT significantly reduced infarcted volume. SMT increased marked survival of HT22 cells against glutamate-induced cytotoxicity in MTT assay. Conclusion: These results suggest that water extract of SMT provides neuroprotection against ischemic or oxidative injury by inhibition of apoptotic cell death.

• Molecules and Cells
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• 제42권11호
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• pp.804-809
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• 2019

Oncogenic gain-of-function mutations are clinical biomarkers for most targeted therapies, as well as represent direct targets for drug treatment. Although loss-of-function mutations involving the tumor suppressor gene, STK11 (LKB1) are important in lung cancer progression, STK11 is not the direct target for anticancer agents. We attempted to identify cancer transcriptome signatures associated with STK11 loss-of-function mutations. Several new sensitive and specific gene expression markers (ENO3, TTC39C, LGALS3, and MAML2) were identified using two orthogonal measures, i.e., fold change and odds ratio analyses of transcriptome data from cell lines and tissue samples. Among the markers identified, the ENO3 gene over-expression was found to be the direct consequence of STK11 loss-of-function. Furthermore, the knockdown of ENO3 expression exhibited selective anticancer effect in STK11 mutant cells compared with STK11 wild type (or recovered) cells. These findings suggest that ENO3-based targeted therapy might be promising for patients with lung cancer harboring STK11 mutations.

• 한국식품영양과학회지
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• 제34권4호
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• pp.524-528
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• 2005

심장질환 등을 유발하는 콜레스테롤을 함유한 동물성 지방을 제조공정을 통하여 제거하고 conventional milk의 기호도와 유사한 cholesterol free 우유를 제조한 후 품질특성을 살펴보았다. 동물성 지방 함량은 대조구와 콜레스테롤을 제거한 실험구 간에 유의적인 차이를 보였으며 (p<0.05), 모든 시료는 알코올, 가수, 체세포수에서 안정성이 있는 품질 특성을 나타내었다. 콜레스테롤은 대조구의 7 mg/100 mL에 비해 실험구는 검출되지 않아 건강에 유익할 수 있는 결과가 나타났다. 모든 시료에서 미생물은 검출되지 않았고, 아플라톡신 $M_1$, 항생물질(7종) 및 합성항균제(15종) 잔류물질도 음성이어서 품질적 차이가 없었다. 색도와 관능검사 중 color는 대조구와 CFM1이 유의성(p<0.05)이 있었으나 CFM2는 차이가 크지 않았다. 결과적으로 본 연구는 실험구 중 CFM2가 보통 우유와 품질적으로 유사한 건강지향적인 cholesterol free 우유의 개발 가능성을 제시하였다.

• 대한한의학회지
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• 제22권1호
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• pp.10-21
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• 2001

Objective : This study was performed to investigate the protective effect of Stephania tetrandra(ST) against ischemic brain damage after a middle cerebral artery(MCA) occlusion. The effect was evaluated using histological tests, neurobehavioral tests, and biochemical tests. Methods : Rats(Sprague-Dawley) were divided into four groups : sham operated group, MCA occluded group, post MCA occlusion Stephania tetrandra administrated (7.6mg/l00g) group, and normal group. The MCA was occluded by intraluminal method. Stephania tetrandra was administrated orally twice at 1 and 4 hours after MCA occlusion. The neurobehavioral test was performed at 3, 6, 9 and 24 hours after MCA occlusion by posture reflex test and swimming behavioral test. All groups were sacrificed then. The brain tissues were stained with 2% triphenyl tetrazolium chloride(TTC) or 1 % cresyl violet solution, to examine infarct size, volume and cell number. Tumor necrosis $factor-{\alpha}$ level was measured from sera using Enzyme-Linked Immunoabsorbent Assay(ELISA). The mRNA expression level of inflammatory cytokines and related receptor type I and II, $IL-1{\beta}$, IL-6, and IL-10 6hours after MCA occlusion were also studied by reverse transcriptase polymerase chain reaction(RTPCR). Results : The results showed that : Stephania tetrandra (1) reduced infarct size and total infarct volume by 52.2% compared to the control group; (2) attenuated significantly in neuronal death, which was shown by a decrease in cell number(P<0.01) and size(P<0.01) in the boundary area of the infarction; (3) significantly reduced serum $TNF-{\alpha}$ level, and increased the mRNA level of IL-10 in the cortex region(P<0.01). However, there was no significant effect on motor deficit in swimming behavioral test. Conclusions : In conclusion, Stephania tetrandra has protective effects against ischemic brain damage at the early stage of ischemia.