• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.70-70
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• 2015

하천에서의 수문/수리적 특성은 주수로의 다양한 지형학적 형태로 나타난다. 특히 흐름과 수반된 유사량의 변화는 하천의 지형학적, 수리기하적 특성을 지배하며, 하도의 물리적 시스템을 변화시켜 동적 평형에 이르게 된다. 하천에서 하도 형성에 지배적인 역할을 하는 수리특성은 하도형성유량으로 지배유량이며, 보통 만제유량을 사용한다. Dunne and Leopold(1978)는 만제유량을 유사의 이송, 하천의 사행, 유선형의 변화 등 하천의 일반적인 형태를 변화시키며, 주수로의 특성을 형성하는 유량으로 정의하였다. 이와 같이 수리 지형학적 특성을 반영하는 만제유량은 하천의 특성을 나타내는 중요한 요소이다. 따라서 본 연구에서는 한강 수계 20개 하천, 27개 수위 관측소의 최소 10년 이상의 실측 자료를 기반으로 다년간의 연평균 실측유량을 산정하였으며, McCandless(2003)가 제시한 지형학적 만제지표를 이용하여 추정한 만제유량과의 상관성을 분석하였다. 추정된 만제유량은 HEC-RAS model을 이용하여 만제하폭, 만제수심, 만제 시 평균유속을 산정하였다. 27개 지점의 실측유량과 만제유량의 상관성 분석결과 만제유량은 연평균 일유량의 7.7배로 나타났다. 따라서 만제유량을 7일 평균유량(1 week mean discharge)으로 정의하였으며, 수정된 7일 유량과 만제유량의 RMSE는 13.90 m/s, MAPE는 9.94 %로 상관성이 매우 높게 나타났다. 또한 만제유량과 만제하폭, 만제수심, 평균유량, 구간경사와 상관성 분석결과 개별적으로의 상관성은 나타나지 않았으나, 만제하폭, 수심, 평균유량과 만제유량에 대한 회귀 분석을 실시한 결과 $R^2$는 0.911로 매우 높게 나타났으며, 구간경사를 추가하여 분석한 결과 $R^2$가 0.914로 증가하였다. 따라서 만제유량은 수리 기하학적 특성이 모두 반영된 하천 특성을 나타내는 복합적인 지표임을 확인하였다. 아울러 만제유량을 통해 추정된 연평균 유량($48{\cdot}Q_{bf}$)을 우리나라의 월간 유출량 분포 비율을 이용하여 일유량으로 배분/생성하였으며, 생성된 일유량을 통해 CCHE2D model을 이용하여 하상변동 모의를 수행하였다. 대상 구간은 병성천 최하류로부터 상류로 7 km 구간이며, 2013년 1월과 12월 측량 자료를 통한 1년간의 실제 하상 변동 자료와 2013년 실측 유량자료에 따른 하상변동 모의 결과 및 만제유량에 의해 배분/생성된 일유량에 따른 하상변동 모의 결과를 비교하였다. 비교 분석 결과 7일 평균 유량으로 정의된 만제유량을 통해 배분/생성된 유량의 수치모의 결과는 실제 측량자료 및 실측유량자료에 따른 하상변동 결과와 매우 일치하는 것을 확인하였다.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.580-580
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• 2015

하천에서의 수문/수리적 특성은 주수로의 다양한 지형학적 형태로 나타난다. 특히 흐름과 수반된 유사량의 변화는 하천의 지형학적, 수리기하적 특성을 지배하며, 하도의 물리적 시스템을 변화시켜 동적 평형에 이르게 된다. 하천에서 하도 형성에 지배적인 역할을 하는 수리특성은 하도형성 유량으로 지배유량이며, 보통 만제유량을 사용한다. Dunne and Leopold(1978)는 만제유량을 유사의 이송, 하천의 사행, 유선형의 변화 등 하천의 일반적인 형태를 변화시키며, 주수로의 특성을 형성하는 유량으로 정의하였다. 이와 같이 수리 지형학적 특성을 반영하는 만제유량은 하천의 특성을 나타내는 중요한 요소이다. 따라서 본 연구에서는 한강 수계 20개 하천, 27개 수위 관측소의 최소 10년 이상의 실측 자료를 기반으로 다년간의 연평균 실측유량을 산정하였으며, McCandless(2003)가 제시한 지형학적 만제지표를 이용하여 추정한 만제유량과의 상관성을 분석하였다. 추정된 만제유량은 HEC-RAS model을 이용하여 만제하폭, 만제수심, 만제 시 평균유속을 산정하였다. 27개 지점의 실측유량과 만제유량의 상관성 분석결과 만제유량은 연평균 일유량의 7.7배로 나타났다. 따라서 만제유량을 7일 평균유량(1 week mean discharge)으로 정의하였으며, 수정된 7일 유량과 만제유량의 RMSE는 13.90m/s, MAPE는 9.94 %로 상관성이 매우 높게 나타났다. 또한 만제유량과 만제하폭, 만제수심, 평균유량, 구간경사와 상관성 분석결과 개별적으로의 상관성은 나타나지 않았으나, 만제하폭, 수심, 평균유량과 만제유량에 대한 회귀 분석을 실시한 결과 $R^2$는 0.911로 매우 높게 나타났으며, 구간경사를 추가하여 분석한 결과 $R^2$가 0.914로 증가하였다. 따라서 만제유량은 수리 기하학적 특성이 모두 반영된 하천 특성을 나타내는 복합적인 지표임을 확인하였다. 아울러 만제유량을 통해 추정된 연평균 유량($48{\cdot}Q_{bf}$)을 우리나라의 월간 유출량 분포 비율을 이용하여 일유량으로 배분/생성하였으며, 생성된 일유량을 통해 CCHE2D model을 이용하여 하상변동 모의를 수행하였다. 대상 구간은 병성천 최하류로부터 상류로 7 km 구간이며, 2013년 1월과 12월 측량 자료를 통한 1년간의 실제 하상 변동 자료와 2013년 실측 유량자료에 따른 하상변동모의 결과 및 만제유량에 의해 배분/생성된 일유량에 따른 하상변동 모의 결과를 비교하였다. 비교 분석 결과 7일 평균 유량으로 정의된 만제유량을 통해 배분/생성된 유량의 수치모의 결과는 실제 측량자료 및 실측유량자료에 따른 하상변동 결과와 매우 일치하는 것을 확인하였다.

• Journal of Veterinary Science
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• v.22 no.3
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• pp.39.18-39.18
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• 2021

Background: Interferon lambda receptor 1 (IFNLR1) is a type II cytokine receptor that clings to interleukins IL-28A, IL29B, and IL-29 referred to as type III IFNs (IFN-λs). IFN-λs act through the JAK-STAT signaling pathway to exert antiviral effects related to preventing and curing an infection. Although the immune function of IFN-λs in virus invasion has been described, the molecular mechanism of IFNLR1 in that process is unclear. Objectives: The purpose of this study was to elucidate the role of IFNLR1 in the pathogenesis and treatment of porcine reproductive and respiratory syndrome virus (PRRSV). Methods: The effects of IFNLR1 on the proliferation of porcine alveolar macrophages (PAMs) during PRRSV infection were investigated using interference and overexpression methods. Results: In this study, the expressions of the IFNLR1 gene in the liver, large intestine, small intestine, kidney, and lung tissues of Dapulian pigs were significantly higher than those in Landrace pigs. It was determined that porcine IFNLR1 overexpression suppresses PRRSV replication. The qRT-PCR results revealed that overexpression of IFNLR1 upregulated antiviral and IFN-stimulated genes. IFNLR1 overexpression inhibits the proliferation of PAMs and upregulation of p-STAT1. By contrast, knockdown of IFNLR1 expression promotes PAMs proliferation. The G0/G1 phase proportion in IFNLR1-overexpressing cells increased, and the opposite change was observed in IFNLR1-underexpressing cells. After inhibition of the JAK/STAT signaling pathway, the G2/M phase proportion in the IFNLR1-overexpressing cells showed a significant increasing trend. In conclusion, overexpression of IFNLR1 induces activation of the JAK/STAT pathway, thereby inhibiting the proliferation of PAMs infected with PRRSV. Conclusion: Expression of the IFNLR1 gene has an important regulatory role in PRRSV-infected PAMs, indicating it has potential as a molecular target in developing a new strategy for the treatment of PRRSV.

• Journal of Life Science
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• v.31 no.12
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• pp.1057-1065
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• 2021

Alterations in mitochondrial DNA (mtDNA) copy numbers have been reported in patients with stomach cancer and suggested to play a role in gastric carcinogenesis or gastric cancer progression. However, changes in the levels of mitochondrial proteins or mtDNA-encoded oxidative phosphorylation (OXPHOS) proteins in gastric cancer remain unclear. In this study, we investigated mtDNA contents, mitochondrial protein levels, and mtDNA-encoded OXPHOS protein levels in gastric cancer tissues and cell lines. We correlated mtDNA copy numbers with clinicopathologic features of the gastric cancer samples used in this study and used quantitative PCR to analyze the mtDNA copy numbers of the gastric cancer tissues and cell lines. Western blot analysis was used for assessing the amounts of mitochondrial proteins and mtDNA-encoded OXPHOS proteins. Among the 27 gastric cancer samples, 22 showed a reduction in mtDNA copy numbers. The mtDNA content was increased in the other five samples relative to that in normal matched gastric tissues. Mitochondrial protein and OXPHOS protein levels were reduced in some gastric cancer tissues. However, mitochondrial protein and OXPHOS protein levels in gastric cancer cell lines were not always in line with their mtDNA contents. The mtDNA copy numbers were reduced in five gastric cancer cell lines tested in this study. In summary, this study reports a common reduction in mtDNA contents in gastric carcinoma tissues and cell lines, pointing to the possible involvement of mtDNA content alterations in tumorigenesis of the stomach.

• Genes and Genomics
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• v.40 no.11
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• pp.1181-1197
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• 2018

Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at $4^{\circ}C$ for 2 h) and LT24 (cold treatment at $4^{\circ}C$ for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.

• Journal of Life Science
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• v.29 no.6
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• pp.645-652
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• 2019

Non-small cell lung cancer (NSCLC) has the infamous distinction of being the leading cause of global cancer-related death over the past decade, and novel molecular targets are urgently required to change this status. We previously conducted a microarray analysis to investigate the association of transcription factor activating enhancer-binding protein 2C (TFAP2C) with NSCLC and revealed its oncogenic roles in NSCLC development. In this study, to identify new biomarkers for NSCLC, we focused on several oncogenes from the microarray analysis that are transcriptionally regulated by TFAP2C. Here, the cell division cycle 20 (CDC20) and tribbles pseudokinase 3 (TRIB3) were subsequently found as potential potent oncogenes as they are positively regulated by TFAP2C. The results showed that the mRNA and protein levels of CDC20 and TRIB3 were down-regulated in two NSCLC cell lines (NCI-H292 and NCI-H838), which were treated with TFAP2C siRNA, and that the overexpression of either CDC20 or TRIB3 was responsible for promoting cell viability in both NSCLC cell lines. In addition, apoptotic levels of NCI-H292 and NCI-H838 cells treated with TFAP2C siRNA were found to be suppressed by the overexpression of either CDC20 or TRIB3. Together, these results suggest that CDC20 and TRIB3 are positively related to NSCLC tumorigenesis and that they should be considered as potential prognostic markers for developing an NSCLC therapy.

• Korean Journal of Veterinary Research
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• v.60 no.4
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• pp.195-202
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• 2020

Feline calicivirus (FCV) infection results in a common upper respiratory disease associated with oral ulceration in cats. Although FCV infection has been reported in cats worldwide, the biologic and genetic features of South Korean FCV are unclear. We aimed to investigate the biological and genetic features of South Korean FCV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FCV from 58 organ homogenate samples. The FCV isolates were confirmed by cytopathic effects, immunofluorescence, electron microscopy, and reverse transcription polymerase chain reaction assays. Viral genetic analysis was carried out with VP2 gene and complete genomes of FCVs. Five viruses propagated in CRFK cells were confirmed to be FCVs. The FCV17D283 isolate showed the highest viral titer of 107.2 TCID50/mL at 36 h post-inoculation. Korean FCV isolates did not grow well in Vero, BHK-21, A72, or Madin-Darby canine kidney cells. The FCV17D03 and FCV17D283 isolates had the highest genetic similarity (80.1% and 86.9%) with the UTCVM-H1 and 14Q315 strains, which were isolated in the United States and South Korea in 1995 and 2014, respectively. We isolated five FCVs from cats and detected important genetic differences among them. FCV isolates did not show any virulent effects in mice.

• The Journal of Advanced Prosthodontics
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• v.13 no.4
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• pp.226-236
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• 2021

PURPOSE. This study aimed to evaluate the effect of incorporating zirconium oxide nanoparticles (nano-ZrO₂) in polymethylmethacrylate (PMMA) denture base resin on flexural properties at different material thicknesses. MATERIALS AND METHODS. Heat polymerized acrylic resin specimens (N = 120) were fabricated and divided into 4 groups according to denture base thickness (2.5 mm, 2.0 mm, 1.5 mm, 1.0 mm). Each group was subdivided into 3 subgroups (n = 10) according to nano-ZrO₂ concentration (0%, 2.5%, and 5%). Flexural strength and elastic modulus were evaluated using a three-point bending test. One-way ANOVA, Tukey's post hoc, and two-way ANOVA were used for data analysis (α = .05). Scanning electron microscopy (SEM) was used for fracture surface analysis and nanoparticles distributions. RESULTS. Groups with 0% nano-ZrO₂ showed no significant difference in the flexural strength as thickness decreased (P = .153). The addition of nano-zirconia significantly increased the flexural strength (P < .001). The highest value was with 5% nano-ZrO₂ and 2 mm-thickness (125.4 ± 18.3 MPa), followed by 5% nano-ZrO₂ and 1.5 mm-thickness (110.3 ± 8.5 MPa). Moreover, the effect of various concentration levels on elastic modulus was statistically significant for 2 mm thickness (P = .001), but the combined effect of thickness and concentration on elastic modulus was insignificant (P = .10). CONCLUSION. Reinforcement of denture base material with nano-ZrO₂ significantly increased flexural strength and modulus of elasticity. Reducing material thickness did not decrease flexural strength when nano-ZrO₂ was incorporated. In clinical practice, when low thickness of denture base material is indicated, PMMA/nano-ZrO₂ could be used with minimum acceptable thickness of 1.5 mm.

• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.800-811
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• 2022

The integration of metabolomics and transcriptomics may elucidate the correlation between the genotypic and phenotypic patterns in organisms. In equine physiology, various metabolite levels vary during exercise, which may be correlated with a modified gene expression pattern of related genes. Integrated metabolomic and transcriptomic studies in horses have not been conducted to date. The objective of this study was to detect the effect of moderate exercise on the metabolomic and transcriptomic levels in horses. In this study, using nuclear magnetic resonance (NMR) spectroscopy, we analyzed the concentrations of metabolites in muscle and plasma; we also determined the gene expression patterns of branched chain (alpha) keto acid dehydrogenase kinase complex (BCKDK), which encodes the key regulatory enzymes in branched-chain amino acid (BCAA) catabolism, in two breeds of horses, Thoroughbred and Jeju, at different time intervals. The concentrations of metabolites in muscle and plasma were measured by ¹H NMR (nuclear magnetic resonance) spectroscopy, and the relative metabolite levels before and after exercise in the two samples were compared. Subsequently, multivariate data analysis based on the metabolic profiles was performed using orthogonal partial least square discriminant analysis (OPLS-DA), and variable important plots and t-test were used for basic statistical analysis. The stress-induced expression patterns of BCKDK genes in horse muscle-derived cells were examined using quantitative reverse transcription polymerase chain reaction (qPCR) to gain insight into the role of transcript in response to exercise stress. In this study, we found higher concentrations of aspartate, leucine, isoleucine, and lysine in the skeletal muscle of Jeju horses than in Thoroughbred horses. In plasma, compared with Jeju horses, Thoroughbred horses had higher levels of alanine and methionine before exercise; whereas post-exercise, lysine levels were increased. Gene expression analysis revealed a decreased expression level of BCKDK in the post-exercise period in Thoroughbred horses.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.5
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• pp.335-345
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• 2022

Pyroptosis is an inflammatory form of programmed cell death that is linked with invading intracellular pathogens. Cardiac pyroptosis has a significant role in coronary microembolization (CME), thus causing myocardial injury. Tanshinone IIA (Tan IIA) has powerful cardioprotective effects. Hence, this study aimed to identify the effect of Tan IIA on CME and its underlying mechanism. Forty Sprague-Dawley (SD) rats were randomly grouped into sham, CME, CME + low-dose Tan IIA, and CME + high-dose Tan IIA groups. Except for the sham group, polyethylene microspheres (42 ㎛) were injected to establish the CME model. The Tan-L and Tan-H groups received intraperitoneal Tan IIA for 7 days before CME. After CME, cardiac function, myocardial histopathology, and serum myocardial injury markers were assessed. The expression of pyroptosis-associated molecules and TLR4/MyD88/NF-κB/NLRP3 cascade was evaluated by qRT-PCR, Western blotting, ELISA, and IHC. Relative to the sham group, CME group's cardiac functions were significantly reduced, with a high level of serum myocardial injury markers, and microinfarct area. Also, the levels of caspase-1 p20, GSDMD-N, IL-18, IL-1β, TLR4, MyD88, p-NF-κB p65, NLRP3, and ASC expression were increased. Relative to the CME group, the Tan-H and Tan-L groups had considerably improved cardiac functions, with a considerably low level of serum myocardial injury markers and microinfarct area. Tan IIA can reduce the levels of pyroptosis-associated mRNA and protein, which may be caused by inhibiting TLR4/MyD88/NF-κB/NLRP3 cascade. In conclusion, Tanshinone IIA can suppress cardiomyocyte pyroptosis probably through modulating the TLR4/MyD88/NF-κB/NLRP3 cascade, lowering cardiac dysfunction, and myocardial damage.