• The Korea Journal of Herbology
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• v.32 no.2
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• pp.77-85
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• 2017

Objectives : This study aimed to investigate the unknown mechanisms behind the anti- inflammatory activity of Hyeonto-dan(HT) 70% ethanol extract on LPS-stimulated RAW 264.7 cells. Methods : Cells were treated with Hyeonto-dan 1 h prior to addition of 200 ng/mL of LPS. Cell viability was measured by the MTS assay. Nitric oxide levels were determined by the Griess assay. $PGE_2$ were measured using EIA kit. Pro-inflammatory cytokine production was measured by the enzyme-linked immunosorbent assay (ELISA). The expression of COX-2, iNOS, and MAPKs was investigated by Western blot, qRT-PCR. $NF-{\kappa}B$/p65 localization and interaction of the TLR-4 receptor with LPS was examined by immunofluorescence assays. Results : Hyeonto-dan had no cytotoxicity at the measured concentration. Hyeonto-dan inhibited NO production and pro-inflammatory cytokines such as IL-6, $TNF-{\alpha}$, and PGE2 as well as the protein and mRNA expression of iNOS and COX-2. Moreover, Hyeonto-dan inhibited the interaction between LPS and TLR-4 in murine macrophages. It suppressed phosphorylation of extracellular signal-regulated kinase (ERK 1/2), c-jun N-terminal kinase (JNK 1/2) and p38. Finally, it inhibited translocation of $NF-{\kappa}B$ in response to competitive LPS. Conclusions : Based on the results of this study, Hyeonto-dan inhibited the binding of TLR-4 receptor to LPS and inhibited the phosphorylation of extracellular signaling pathway MAPKs. These inhibitory effects are thought that the amount of $NF-{\kappa}B$ delivered to the nucleus was decreased and the inflammatory reaction was prevented by decreasing the production of LPS-induced $PGE_2$, NO, IL-6 and $TNF-{\alpha}$.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.72-86
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• 2022

This study was conducted to identify structural characteristics of the evergreen broad-leaved forests distributed in Hak-dong, Geojae island. For a survey, 52 sites were set up in areas with changes in the vegetation community or location environment where Cinnamomum yabunikkei, Neolitsea sericea, and Machilus thunbergii dominated or appeared in the canopy, sub-canopy, or shrub layer. The community classification with TWINSPAN identified the following communities: N. sericea-C. yabunikkei, C. yabunikkei-Camellia japonica, Ca. japonica, Quercus variabilis-Ca. japonica, Pinus thunbergii-Ca. japonica, Castanopsis sieboldii, P. thunbergii, and Platycarya strobilacea-Mallotus japonicus. Considering the result of the study that succession series of warm-temperate forest reflecting the latent natural vegetation is the transition of conifers and deciduous broad-leaved forest to evergreen broad-leaved forest, the communities predominated by the communities predominated by the communities predominated by P. thunbergii, Q. variabilis, and Pl. strobilacea are likely to transform into the evergreen forest predominated by N. sericea and C. yabunikkei. The sites where C. yabunikkei, N. sericea, and Castanopsis sieboldii are dominant in the canopy and sub-canopy layers are likely to maintain the status quo if there is no artificial disturbance. The relationship between the impact of the environmental factors and the vegetation distribution showed silt among the physical properties of the soil directly or indirectly affected it, which was judged to be due to the fact that it was located on a steep slope. The soil acidity (pH) was 5-5.84, electrical conductivity 0.047-0.139 dS/m, and organic matter content was 3.32-12.06%. Although there were differences by the colony, they were generally low.

• Journal of the Korean Geotechnical Society
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• v.25 no.5
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• pp.75-86
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• 2009

Salt, one of the most common soluble materials in engineering soil, may have an effect on mechanical behaviors of soils under its cementation process. In order to investigate this natural phenomenon, non-soluble material by using glass beads is mixed with salt electrolyte and cemented by using oven to evaporate water. Three different sizes of glass bead particles, 0.26, 0.5, and 1.29 mm, with different salt concentration, 0, 0.1, 0.2, 0.5, 1.0, and 2.0M, are explored by using P- and S-waves, excited by bender elements and piezo disk elemets, respectively. The velocities of the P-wave and S-wave of the particulate medium cemented by salt show three stages with the degree of saturation: 1) S-wave velocities increase while P-wave velocities reduce with degree of saturation changing from 100% to 90%; 2) Both velocities are stable with degree of saturation varying from 90% to 10%; 3) The velocities change enormously when the specimens are nearly dry with degree of saturation from 10% to 0%. Besides, the resonance frequencies of S-wave show similar stages to the S-wave velocities. This study demonstrates meaningful trends of elastic wave characteristics of geo-materials according to the cementation of dissolved salt.

• Animal Bioscience
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• v.35 no.10
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• pp.1489-1498
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• 2022

Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep. Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population. Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes. Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

• Biomolecules & Therapeutics
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• v.32 no.1
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• pp.154-161
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• 2024

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder that causes progressive paralysis. L-Citrulline is a nonessential neutral amino acid produced by L-arginine via nitric oxide synthase (NOS). According to previous studies, the pathogenesis of ALS entails glutamate toxicity, oxidative stress, protein misfolding, and neurofilament disruption. In addition, L-citrulline prevents neuronal cell death in brain ischemia; therefore, we investigated the change in the transport of L-citrulline under various pathological conditions in a cell line model of ALS. We examined the uptake of [¹⁴C]L-citrulline in wild-type (hSOD1wt/WT) and mutant NSC-34/ SOD1^G93A (MT) cell lines. The cell viability was determined via MTT assay. A transport study was performed to determine the uptake of [¹⁴C]L-citrulline. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was performed to determine the expression levels of rat large neutral amino acid transported 1 (rLAT1) in ALS cell lines. Nitric oxide (NO) assay was performed using Griess reagent. L-Citrulline had a restorative effect on glutamate induced cell death, and increased [¹⁴C]L-citrulline uptake and mRNA levels of the large neutral amino acid transporter (LAT1) in the glutamate-treated ALS disease model (MT). NO levels increased significantly when MT cells were pretreated with glutamate for 24 h and restored by co-treatment with L-citrulline. Co-treatment of MT cells with L-arginine, an NO donor, increased NO levels. NSC-34 cells exposed to high glucose conditions showed a significant increase in [¹⁴C]L-citrulline uptake and LAT1 mRNA expression levels, which were restored to normal levels upon co-treatment with unlabeled L-citrulline. In contrast, exposure of the MT cell line to tumor necrosis factor alpha, lipopolysaccharides, and hypertonic condition decreased the uptake significantly which was restored to the normal level by co-treating with unlabeled L-citrulline. L-Citrulline can restore NO levels and cellular uptake in ALS-affected cells with glutamate cytotoxicity, pro-inflammatory cytokines, or other pathological states, suggesting that L-citrulline supplementation in ALS may play a key role in providing neuroprotection.

• The Korean Journal of Malacology
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• v.24 no.2
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• pp.153-159
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• 2008

We investigated artificial mass seed production of a Chinese scallop, Patinopecten yessoensis, in 2004. The GSI(gonad somatic index) of the Chinese scallop, P yessoensis was 17.2 on mid-February, 20.2 on mid-March, while that of Korean scallop, P yessoensis was 6.9 on mid-February, 10.8 on mid-March. Matured 120 females and 350 males were selected for artificial mass production. They were exposed in air for 1 hr at over $20^{\circ}C$, and placed into a spawning tank(20 ton) containing sea water treated with UV radiation at $12^{\circ}C$. We gained a total of 228,000 thousand scallop embryos between March 10th and 15th, and reared larvae at the indoor tank during 25 days. When the mean shell length of larvae reached 250 ${\mu}m$ and they have eye-spots, the number of pre-settling larvae was 47,500 thousand. We gained 1,850 thousand attached scallop spats from two kinds of collectors. Attached spats were reared in indoor tank for different periods from 5 days to 60 days. They were divided into 5 groups according to the length of reared days. Each group of attached spats was moved to intermediate rearing sites at Yangyang fishing port in Gangreung-city for acclimation to ocean environments. The highest survival rate of attached spats was 13.0% shown at the group reared for 12 days, but the significant difference in their growth was not found between the groups. The shell length of artificial attached spats increased from 0.9 ${\mu}m$ on July 10th to 24.7 ${\mu}m$ on December 16th with the survival rate of 85.0% while that of natural attached spats increased from 0.6 ${\mu}m$ on July 10th to 23.9 ${\mu}m$ on December 16th with the survival rate of 85.7%.

• Tuberculosis and Respiratory Diseases
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• v.41 no.2
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• pp.111-119
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• 1994

Background: Because of the common etiologic factor, such as smoking, lung cancer and chronic obstructive pulmonary disease are often present in the same patient. The preoperative prediction of remaining pulmonary function after the resectional surgery is very important to prevent serious complication and postoperative respiratory failure. $^{99m}Tc$-MAA perfusion scan has been used for the prediction of postoperative pulmonary function, but it may be inaccurate in case of large V/Q mismatching. We compared $^{99m}Tc$-DTPA radioaerosol inhalation scan with $^{99m}Tc$-MAA perfusion scan in predicting postoperative lung function. Method: Preoperative inhalation scan and/or perfusion scan were performed and pulmonary function test were performed preoperatively and 2 month after operation. We predicted the postoperative pulmonary functions using the following equations. Postpneurnonectomy $FEV_1$=Preop $FEV_1x%$ of total function of lung to remain Postlobectomy $FEV_1$=Preop $FEV_1{\times}$(% of total 1-function of affected lung${\times}$$\frac{Number\;of\;segments\;to\;be\;resected}{Number\;of\;segments\;of\;affected\;lung})$ Results: 1) The inhalation scan showed good correlations between measured and predicted $FEV_1$, FVC and $FEF_{25-75%}$. (correlation coefficiency; 0.94, 0.91, 0.87 respectively). 2) The perfusion scan also showed good correlations between measured and predicted $FEV_1$, FVC and $FEF_{25-75%}$. (correlation coefficiency; 0.86, 0.72, 0.87 respectively). 3) Among three parameters, $FEV_1$ showed the best correlations in the prediction by lung scans. 4) Comparison between inhalation scan and perfusion scan in predicting pulmonary function did not show any significant differneces except FVC. Conclusion: The inhalation scan and perfusion scan are very useful in the prediction of postoperative lung function and don't make a difference in the prediction of pulmonary function a1though the former showed a better correlation in FVC.

• Korean Journal of Environment and Ecology
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• v.29 no.4
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• pp.570-579
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• 2015

Lindera sericea, which belongs to genus Lindera in the Lauraceae family, is labeled under Least Concerned (LC) among Korean rare plants, floristics specific species IV grade and also flagpole species of the Mudeung National Park. It is distributed in Mt. Mudeung, Mt. Cheonbong and Mt. Chogye within gradients from $12^{\circ}$ to $51^{\circ}$. The slope area is from northeast to northwest sides and the altitude range of distribution site is between 220 m and 533 m. The vascular plants in the quadrate where Lindera sericea were studied were identified as a total of 72 taxa; 37 families, 54 genera, 66 species, 5 variants and 1 forma. Among the floristics specific species, IV grade species was Lindera sericea and III grade species were Stewartia pseudocamellia and Acer palmatum. Korean endemic species were Stewartia pseudocamellia and Carex okamotoi. As for the vegetation group, Quercus mongolica and Q. serratak - Lindera sericea, Styrax japonicus populations were found in Mt. Mudeung area where Lindera sericea appeared, Stewartia pseudocamellia-Lindera sericea and Sasa borealis populations were found in Mt. Cheonbong areas, Carpinus laxiflora - Lindera sericea and Sasa borealis populations were found in Mt. Chogye area, and Stewartia pseudocamellia-Lindera sericea and Sasa borealis populations were found in Songgwangsa area. A total number of 662 Lindera sericea individuals were examined. The number of trees with 1 trunk including younger individuals was 353 (53.32%), and the number of trees with 2 to 5 stems was 270 (40.79%). The number of trees with the greatest number of sprouts was 27. Of 662 trees in total, the total number of sprouts was 1,198. Among these, 699 trees (58.34%) were between 50 cm and 150 cm in height. The tallest tree was 585 cm. The most common root-collar diameter of sprouts (992, 82.81%) was under 1 cm, followed by the sprouts with collar diameter from 1.0 to 1.5 cm(156, 13.2%). Among them, the largest root-collar diameter was 3.2 cm. This Lindera sericea specimen had the tallest trunk (565 cm) which was torn lengthwise on one side. Its root and breast parts were decayed by 50% and 25 respectively. Some branches of the three trunks that were more than 4 m in length were dead in the apical portion. Therefore, it is proposed that the Korean Lindera sericea maintains an apical dominance tree type while showing morphological adaptation as a typical shrub because it autonomously decays some branches and trunks over a certain height while increasing the number of its sprouts.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.89-119
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• 2004

This study was conducted to isolate lactobacilli having probiotic characteristics to be used as health adjuncts with fermented milk products. Acid tolerant strains were selected in Lactobacilli MRS broth adjusted to pH 4.0 from 80 healthy persons (infants, children and adults). And bile tolerant strains were examined in Lactobacilli MRS broth in which 1.0% bile salt was added. By estimation above characteristics, the strains No. 27, which was isolated from adult feces, was selected and identified as Lactobacillus salivarius subsp. salivarius based on carbohydrate fermentation and 16S rDNA sequencing. It was used as a probiotic strain in fermented milk products. The pH of fermented milk decreased from pH 6.7 to 5.0 and titratable acidity increased from 0.3% to 1.0% by L. salivarius subsp. salivarius (isolation strain 20, 35, and 37), when incubated for 36 h at $37^{\circ}C$. The number of viable cell counts of fermented milk was maximized at this incubation condition. The SDS-PAGE evidenced no significant change of casein but distinct changes of whey protein were observed by isolated L. salivarius subsp. salivarius for titratable acidity being incubated by $0.9{\sim}1.0%$ at $37^{\circ}C$. All of the strains produced 83.43 to 131.96 mM of lactic acid and 5.39 to 26.85 mM of isobutyric acid in fermented products. The in vitro culture experiment was performed to evaluate ability to reduce cholesterol levels and antimicrobial activity in the growth medium. The selected L. salivarius subsp. salivarius reduced $23{\sim}38%$ of cholesterol content in lactobacilli MRS broth during bacterial growth for 24 hours at $37^{\circ}C$. All of the isolated L. salivarius subsp. salivarius had an excellent antibacterial activity with $15{\sim}25$ mm of inhibition zone to E. coli KCTC1039, S. enteritidis KCCM3313, S. typhimurium M-15, and S. typhimurium KCCM40253 when its pH had not been adjusted. Also, all of the isolated L. salivarius subsp. salivarius had partial inhibition zone to E. coli KCTC1039, E. coli KCTC0115 and S. enteritidis KCCM3313 when it had been adjusted to pH 5.7. The selected strains were determined to have resistances of twelve antibiotic. Strains 27 and 35 among the L. salivarius subsp. salivarius showed the highest resistance to the antibiotics. Purified ${\alpha}$-galactosidase was obtained by DEAE-Sephadex A-50 ion exchange chromatography, Mono-Q ion exchange chromatography and HPLC column chromatography from L. salivarius subsp. salivarius 27. The specific activity of the purified enzyme was 8,994 units/mg protein, representing an 17.09 folds purification of the original cell crude extract. The molecular weight of enzyme was identified about 53,000 dalton by 12% SDS-PAGE. Optimal temperature and pH for activity of this enzyme were $40^{\circ}C$ and 7.0 respectively. The enzyme was found to be stable between 25 and $50^{\circ}C$. ${\alpha}$-galactosidase activity was lost rapidly below pH 5.0 and above pH 9.0. This enzyme was liberated galactose from melibiose, raffinose, and stachyose, and also the hydrolysis rate of substrate was compound by HPLC. These results indicated that some of the L. salivarius subsp. salivarius (strain 27 and 35) are considered as effective probiotic strains with a potential for industrial applications, but the further study is needed to establish their use as probiotics in vivo.

• Korean Journal of Environment and Ecology
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• v.27 no.1
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• pp.113-126
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• 2013

This study was performed to introduce ecological education program as for reporting the current vegetation state within the remaining trees as ecological golf course and to provide basic data. The survey site was S Golf course, which is located in Woonseodong, Incheon city. Its total area was about $3,298,428m^2$, but the relict forest was about $225,143m^2$. Existing landuse, topological structure, the flora, actual vegetation, and plants community structure survey were performed within the relict forest. As result of comparing and analyzing the existing land use, the relict forest was distributed in the forested areas (89.2%) and around the area (10.8%) which had been bare land and SAMMOK earthen ramparts. There were two courses (Ocean and Hanul) with the relict forests. The ocean course was compared of a natural forest, such as Quercus spp. mixed forest, Quercus acutissima forest, Pinus thunbergii forest within the rock fields, and an artificial forest (Ailanthus altissima-Robinia pseudoacacia forest, Robinia pseudoacacia forest) and Quercus acutissima - Elaeagnus umbellata forest. On the Hanul course, Pinus rigida forest and Robinia pseudoacacia forest were the main vegetation, which were artificial forest. It was the contrast aspect of vegetation species in a natural forest, a restoration forest and an artificial forest, which were Q. spp. mixed forest 26~28 species in a natural forest within the vegetation type per investigation area, 3 Pinus thunbergii forest species, and 5~7 artificial forest species on the Hanul course. Based on these vegetation status, the Ocean course was designed into ecological theme spaces named 'Quercus spp.' indigenous forest, 'Pinus thunbergii' restoration forest and ecological story of 'SAMMOK earthen ramparts'. The Hannul course was designed into an artificial forest observation area of 'Robinia pseudoacacia' and 'Pinus rigida' and a fragrance forest area of 'Robinia pseudoacacia'. At the time of the discussion about the introduction of eco-friendly approval system of golf course, it would be estimated that this survey would work as a major material not only raising awareness of the golf course on the ecological environment but also providing programs that can contribute to the community.