• Korean Journal of Remote Sensing
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• v.31 no.2
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• pp.51-64
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• 2015

We improved the MODerate resolution Imaging Spectroradiometer (MODIS) land cover map over the Asia-Oceania region through the reclassification of the misclassified pixels. The misclassified pixels are defined where the number of land cover types are greater than 3 from the 12 years of MODIS land cover map. The ratio of misclassified pixels in this region amounts to 17.53%. The MODIS Normalized Difference Vegetation Index (NDVI) time series over the correctly classified pixels showed that continuous variation with time without noises. However, there are so many unreasonable fluctuations in the NDVI time series for the misclassified pixels. To improve the quality of input data for the reclassification, we corrected the MODIS NDVI using Correction based on Spatial and Temporal Continuity (CSaTC) developed by Cho and Suh (2013). Iterative Self-Organizing Data Analysis (ISODATA) was used for the clustering of NDVI data over the misclassified pixels and land cover types was determined based on the seasonal variation pattern of NDVI. The final land cover map was generated through the merging of correctly classified MODIS land cover map and reclassified land cover map. The validation results using the 138 ground truth data showed that the overall accuracy of classification is improved from 68% of original MODIS land cover map to 74% of reclassified land cover map.

• Biomolecules & Therapeutics
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• v.23 no.5
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• pp.486-492
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• 2015

Drug metabolism mostly occurs in the liver. Cytochrome P450 (CYP) is a drug-metabolizing enzyme that is responsible for many important drug metabolism reactions. Recently, the US FDA and EU EMA have suggested that CYP enzyme induction can be measured by both enzymatic activity and mRNA expression. However, these experiments are time-consuming and their interassay variability can lead to misinterpretations of the results. To resolve these problems and establish a more powerful method to measure CYP induction, we determined CYP induction by using luminescent assay. Luminescent CYP assays link CYP enzyme activity to firefly luciferase luminescence technology. In this study, we measured the induction of CYP isozymes (1A2, 2B6, 2C9, and 3A4) in cryopreserved human hepatocytes (HMC424, 478, and 493) using a luminometer. We then examined the potential induction abilities (unknown so far) of mesalazine, a drug for colitis, and mosapride citrate, which is used as an antispasmodic drug. The results showed that mesalazine promotes CYP2B6 and 3A4 activities, while mosapride citrate promotes CYP1A2, 2B6, and 3A4 activities. Luminescent CYP assays offer rapid and safe advantages over LC-MS/MS and qRT-PCR methods. Furthermore, luminescent CYP assays decrease the interference between the optical properties of the test compound and the CYP substrates. Therefore, luminescent CYP assays are less labor intensive, rapid, and can be used as robust tools for high-throughput CYP screening during early drug discovery.

• Journal of the Korean Vacuum Society
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• v.16 no.4
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• pp.291-297
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• 2007

The field emission properties of CNT-emitters coated with Ag-Cu alloy have been investigated. The vertical aligned multi-walled CNTs were synthesized by dc-plasma enhanced chemical vapor deposition (dc-PECVD) and the Ag-Cu alloy was coated by using dc-magnetron sputter. The morphology of alloy-coated and un-coated CNT-emitters was observed by using SEM and their field emission properties were also measured. Annealing the AgCu-coated CNTs at temperature more than ${\sim}700^{\circ}C$, the Ag-Cu alloy was diffused to and aggregated on the top of the CNT as a Q-tip. A significant progress on the field emission was not observed with coating Ag-Cu alloy on the CNTs, but a certain improvement in a resistance against oxygen gas was made confirmation. It seems to be due to inertness of Ag-Cu alloy on the CNTs.

• Journal of Animal Science and Technology
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• v.58 no.2
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• pp.6.1-6.11
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• 2016

Background: Dietary supplementation of unsaturated fats in ruminants, if not stabilized, can instigate oxidative stress which can have negative impact on production performance and enhance the susceptibility to various diseases. The current study examined the effect of dietary 80 % canola oil and 20 % palm oil blend (CPOB) on serum fatty acids, antioxidant profile and biochemical indices in goats. Thirty Boer bucks (4-5 months old; initial BW, $20.34{\pm}0.77kg$) were randomly assigned to diets containing 0, 4 or 8 % CPOB and fed daily for a period of 90 days. Blood was sampled from the goats on 0, 30, 60 and 90 days of the trial and the serum was analyzed for fatty acids, cholesterol, glucose, total protein, antioxidants and lipid oxidation. Results: Neither diet nor sampling time influenced serum TBARS value, catalase, glutathione peroxidase and superoxide dismutase activities, LDL cholesterol, VLDL cholesterol, triglycerides, glucose and total protein. Goats fed 4 and 8 % CPOB had higher (P < 0.05) total cholesterol and HDL cholesterol than the control goats on day 30, 60 and 90. The proportion of C15:0 decreased with increasing level of CPOB on day 30 and 60. Serum C18:1n-9 increased with increasing level of CPOB in diet on day 60. The proportion of C18:3n-3 and C22:5n-3 increased (P < 0.05), while the proportion of C18:2n-6 decreased (P < 0.05) with increase in the level of CPOB on day 60 and 90. Dietary CPOB did not affect serum total carotenoid and ${\delta}$-tocopherol but did increase (P < 0.05) ${\alpha}$ and ${\gamma}$-tocopherol. Conclusion: Dietary canola oil and palm oil blend could be supplemented in diets without instigating oxidative stress in goats.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.2 no.1
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• pp.77-79
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• 2002

The urea cycle, consisting of a series of six enzymatic reactions, plays key roles to prevent the accumulation of toxic nitrogenous compound and synthesize arginine de novo. Five well characterized diseases have been described, resulting from an enzymatic defect in the biosynthesis of one of the normally expressed enzyme. This presentation will focus on two representative diseases; ornithine transcarbamylase(OTC) deficiency and citrullinemia(argininosuccinate synthetase deficiency). OTC deficiency is one of the most common inborn error of urea cycle, which is inherited in X-linked manner. We identified 17 different mutations in 20 unrelated Korean patients with OTC deficiency; L9X, R26P, R26X, T44I, R92X, G100R, R141Q, G195R, M205T, H214Y, D249G, R277W, F281S, 853 del C, R320X, V323M and 10 bp del at nt. 796-805. These mutations occur at well conserved nucleotide sequences across species or CpG hot spot. The L9X and R26X lead to the disruption of leader sequences, required for directing mitochondrial localization of the OTC precursor. Their phenotypes are severe, and neonatal onset. The G100R, R277W and V323M mutations were uniquely identified in patients with late onset OTC deficiency. The other genotypes are associated with neonatal onset. Out of 20 patients with OTC deficiency, only 6 patients are alive; two were liver transplanted, and normal in growth and development at 2, 4 years after transplantation respectively. Citrullinemia is an autosomal recessive disease, caused by the mutations in the argininosuccinate synthetase(ASS) gene. We identified in 3 major mutations in 11 unrelated Korean patients with citrullinemia; G324S, $IVS6^{-2}$ A to G, and 67 bp ins at nt 1125-1126. Among these, the 67 base pair insertion mutation is novel. The allele frequency of each mutation is; G324S(45%), IVS6-2 A to G(32%), and 67 base pair insertion(14%). All patients are diagnosed at neonatal or infantile age. Interestingly, two patients presented with stroke like episode. Out of 11 patients, 5 patients died. Among 6 patients alive, one patient was successfully liver transplanted.

• Korean Journal of Ecology and Environment
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• v.50 no.1
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• pp.126-136
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• 2017

We describe characteristics of diversity and distribution of oak forests on the Daegok-cheon gorge of the Southeastern Korean Peninsula, the oldest prehistoric site, in view of a sort of food resources of acorns. The $Z{\ddot{u}}rich$-Montpellier School's method was adopted for field investigation on the oak dominant stands. A total of 20 phytosociological $relev\acute{e}s$ composed of 193 taxa were analyzed by syntaxonomy and ecological flora. The Daegok-cheon's oak forests occupied 36.9% ($513,374m^2$) of the surveyed area, and its 99% ($507,677m^2$) was Quercus variabilis and Q. serrata stands. Oak forests of the Daegok-cheon gorge are a kind of regional vegetation type characterizing by the high relative net contribution degree (r-NCD) of Platycarya strobilacea and Sapium japonicum, which are an edaphic type of the xerophilous and thermophilic oak forests. The region of the Daegok-cheon petroglyphs is defined as an ecoregion with rich acorn supply and abundant water resources, and the warmer environment, which attracts prehistoric man.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.5
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• pp.1564-1572
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• 2010

In this study, the falls detection and direction classification system was implemented using 3-axial acceleration signal. The acceleration signals were acquired from the 3-axial accelerometer(MMA7260Q, Freescale, USA), and then transmitted to the computer through USB interface. The implemented system can detect falls using the newly proposed algorithm, and also classify the direction of falls using fuzzy classifier. The 6 subjects was selected for experiment and the accelerometer was attached on each subject's chest. Each subject walked in normal pace for 5 seconds, and then the fall down according to the four direction(front_fall, back_fall, left_fall and right_fall) during at least 2 second. The falls was easily detect using the newly proposed algorithm in this study. The acquired signals were analyzed after 1 second from generating falls. The fuzzy classifier was used to classify the direction of falls. The mean value of the falls detection rate was 94.79%. The classifier rate according to falls direction were 95.83% in case of front falls, 100% incase of back falls, 87.5% in case of left falls, and 95.83% in case of right falls.

• The Journal of Engineering Geology
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• v.30 no.2
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• pp.161-173
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• 2020

This study is to examine the engineering characteristics of colunmar joints in Mudeugsan National Park, a global geopark. For these purposes, physical and mechanical properties of Mudeungsan Tuff, evaluation for the weathering degree of columnar joints, and crack behavior monitoring in columnar joints were conducted. The physical properties of Mudeungsan tuff were 1.02% for the average porosity, 0.38% for the average absorption, 2.69 g/㎤ for the average specific gravity, and 4,948 m/s for the average elastic wave velocity. Its mechanical properties were 337 MPa for the average uniaxial compressive strength, 68 GPa for the average elastic modulus, 0.29 for the average Poisson's ratio, 41.3 MPa for the average cohesion strength, and 62.8° for the average friction angle. the average rebound Q-value of the silver Schmidt hammer for the three columnar joint blocks at the Ipseok-dae was shown as 49.3. when this value is converted into uniaxial compressive strength, it becomes 70.5 MPa, which is about 21% of the uniaxial compression strength of Mudeungsan tuff. In addition, according to the results of crack monitoring measurements for the three columnar joint blocks at the Ipseok-dae, the crack behavior is less than 1 mm, so it is believed that its behavior in Ipseak-dae columnar joints has hardly occured to date.

• Genomics & Informatics
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• v.9 no.4
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• pp.152-160
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• 2011

Hypertension is the major factor of most death and high blood pressure (BP) can lead to stroke, myocardial infarction and cardiac failure. Moreover, hypertension is strongly correlated with body mass index (BMI). Although the exact causes of hypertension are still unclear, some of genetic loci were discovered from genome-wide association study (GWAS). Therefore, it is essential to study genetic variation for finding more genetic factor affecting hypertension. The purpose of our study is to conduct a CNV association study for hypertension-related traits, BP and BMI, in Korean individuals. We identified 2,206 CNV regions from 3,274 community-based Korean participants using the Affymetrix Genome-Wide Human SNP Array 6.0 platform and performed a logistic regression analysis of CNVs with two hypertension-related traits, BP and BMI. Moreover, the 4,692 participants in an independent cohort were selected for respective replication analyses. GWAS of CNV identified two loci encompassing previously known hypertension-related genes: LPA (lipoprotein) on 6q26, and JAK2 (Janus kinase 2) on 9p24, with suggestive p-values (0.0334 for LPA and 0.0305 for JAK2 ). These two positive findings, however, were not evaluated in the replication stage. Our result confirmed the conclusion of CNV study from the WTCCC suggesting weak association with common diseases. This is the first study of CNV association study with BP and BMI in Korean population and it provides a state of CNV association study with common human diseases using SNP array.

• Journal of Oral Medicine and Pain
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• v.38 no.2
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• pp.215-219
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• 2013

The descending control system is composed of a group of structures in the midbrain, medulla oblongata and pons that form a network of descending inhibitory projections. In the clinical setting, it has been shown that the application of a electrical counterirritant to these structures and diminishes the pain in patients. Thus, depression and anxiety have been shown to predict the development of chronic neuropathic pain state. These factors could influence pain might also involve descending controls. Interestingly, reduced descending controls are seen in patients with irritable bowel syndrome and theses patients had greater anxiety, depression compared to controls. And, the influence of anxiety on the chronicity of pain and on the descending control pathways should be tested in animal models, using modern techniques. Given this Knowledge, it is no wonder that pain is a highly personal experience that is susceptible to a variety of biologic, pharmacologic, and environmental influences.