• 한국식품영양과학회지
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• 제42권5호
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• pp.784-791
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• 2013

시판 효모 4가지(Fermivin, FM; Saf-instant yeast red, SI; Angest wine active dry yeast, AW; Angest instant yeast high sugar, AI)를 이용하여 복분자 발효주를 제조하고, 품질 특성을 비교하였다. 환원당 함량은 FM(2.7%)과 AI(2.8%)가 SI(2.4%)와 AW(2.5%)보다 높았으며, 검출된 주요 유리당인 glucose의 함량은 AW와 AI에서 유의적으로 높았다. 알코올 함량은 AW(11.95%)에서 가장 높고, SI(11.75%)에서 가장 낮았다. pH는 FM(pH 3.73)에서 가장 낮았으며, 총 산도는 효모별로 차이가 없었다. 모든 시료에서 검출된 주요 유기산은 citric acid였으며, 효모별로 검출된 유기산 함량은 달랐다. Malic acid의 경우 SI(2.92 mg/mL)에서 가장 많았고 AI(1.83 mg/mL)에서 가장 적었다. L, a, b 값은 SI에서 높고 AI에서 낮았으며, 탁도는 효모에 따라 차이가 없었다. 복분자 발효주의 total phenolics와 total anthocyanin 함량은 효모 별로 차이가 없었으나, 국내에서 판매되는 수입산 레드 와인보다 높았다. 항산화능 역시 효모별로 차이가 없었으나 FM, AI, AW, SI 순으로 높았으며, 항산화능 지표에는 total phenolics와 anthocyanin의 함량뿐만 아니라 anthocyanin 조성도 영향을 미쳤다.

• International Journal of Oral Biology
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• 제32권1호
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• pp.35-43
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• 2007

Tooth loss in elderly is mainly caused by alveolar bone loss via severe periodontitis. Although the severity of periodontitis is known to be affected by age, the aging process or the genetic changes during the aging of periodontal tissue cells are not well characterized. In this study, we investigated the effect of in vitro aging on the change of gene expression pattern in periodontal fibroblasts. Gingival fibroblasts (GF) and periodontal ligament fibroblasts (PDL) were obtained from two young patients and replicative senescence was induced by sequential subcultivation. When more than 90% cells were positively stained with senescence-associated ${\beta},-galactosidase$, those cells were regarded as aged cells. In aged GF and PDL, the level of phosphorylated retinoblastoma (RB) and $p16^{INK4A}$ protein was significantly decreased and increased, respectively. However, the protein level of p53 and p21, well known senescence-inducing genes, did not increase in aged GF and PDL. Although $p27^{Kip1}$ and $p15^{INK4B}$, another cyclin-dependent kinase inhibitors, were reported to be involved in replicative senescence of human cells, they were decreased in aged GF and PDL. Because senescent cells showed flattened and enlarged cell shape and are known to have increased focal adhesion, we examined the protein level of several integrins. Aged GF and PDL showed increased protein level of integrin ${\alpha}2$, ${\alpha}v$, and ${\beta}1$. When the gene expression profiles of actively proliferating young cells and aged cells were compared by cDNA microarray of 3,063 genes and were confirmed by reverse transcription-polymerase chain reaction, 7 genes and 15 genes were significantly and commonly increased and decreased, respectively, in aged GF and PDL. Among them, included are the genes that were known to be involved in the regulation of cell cycle, gene transcription, or integrin signaling. The change of gene expression pattern in GF and PDL was minimally similar to that of oral keratinocyte. These results suggest that $p16^{INK4A}/RB$ might be involved in replicative senescence of periodontal fibroblasts and the change of gene expression profile during aging process is cell type specific.

• Asian-Australasian Journal of Animal Sciences
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• 제31권9호
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• pp.1401-1406
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• 2018

Objective: The uncoupling protein 3 (UCP3) is a member of the mitochondrial anion carrier superfamily and has crucial effects on growth and feed efficiency in many species. Therefore, the objective of the present study was to examine the association of polymorphisms in the UCP3 gene with feed efficiency in meat-type chickens. Methods: Six single nucleotide polymorphisms (SNPs) of the UCP3 gene were chosen to be genotyped using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry in meat-type chicken populations with 724 birds in total. Body weight at 49 (BW49) and 70 days of age (BW70) and feed intake (FI) in the interval were collected, then body weight gain (BWG) and feed conversion ratio (FCR) were calculated individually. Results: One SNP with a low minor allele frequency (<1%) was removed by quality control and data filtering. The results showed that rs13997809 of UCP3 was significantly associated with BWG and FCR (p<0.05), and that rs13997811 had significant effects on BW70 and BWG (p<0.05). Rs13997812 of UCP3 was strongly associated with BW70, FI, and FCR (p<0.05). Furthermore, individuals with AA genotype of rs13997809 had significantly higher BWG and lower FCR (p<0.05) than those with AT genotype. The GG individuals showed strongly higher BW70 and BWG than AA birds in rs13997811 (p<0.05). Birds with the TT genotype of rs13997812 had significantly greater BW70 and lower FCR compared with the CT birds (p<0.05). In addition, the TAC haplotype based on rs13997809, rs13997811, and rs13997812 showed significant effects on BW70, FI, and FCR (p<0.05). Conclusion: Our results therefore demonstrate important roles for UCP3 polymorphisms in growth and feed efficiency that might be used in meat-type chicken breeding programs.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.301-305
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• 2002

The gene (ppaT) encoding Thermus caldophilus GK24 pyrophosphatase (Tca pyrophosphatase) was cloned and sequenced. The gene was found to contain an open reading frame encoding 175 amino acids with a calculated mass of 19,155 Da. The ppaT gene was expressed under the control of the tac promoter in Escherichia coli. The recombinant Tca pyrophosphatase was purified 21.4-fold with $56\%$ yield and specific activity of 25.7 U $mg^-1$, following a combination of heating (to denature the E. coli proteins) and one step of DEAE-Sephacel column chromatography. The native enzyme was found to have an approximate molecular mass of 110,000 Da and consisted of six subunits. The enzyme exhibited maximal activity at pH of 8.0-8.5 and was stable at $80-90^{\circ}C$. A divalent cation was absolutely required for the enzyme activity, with $Mg^2+$. being the most effective.

• The Korean Journal of Pain
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• 제24권4호
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• pp.216-220
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• 2011

Background: Transforaminal epidural steroid injection is one of the effective treatments in managing radicular pain. There have been some prospective studies on the depth to the epidural space with the transforaminal approach. However, there have been no studies about the depth in Asians, especially Koreans. This study was carried out in order to evaluate the depth to the epidural space and the oblique angle and factors that influence the depth to the epidural space during lumbar transforaminal epidural injection. Methods: A total of 248 patients undergoing fluoroscopically guided transforaminal epidural steroid injections were evaluated. At the L3-4, L4-5, L5-S1, and S1 levels, we measured the oblique angle and depth to the epidural space. Results: Needle depth was positively associated with body mass index (correlation coefficient 0.52, P = 0.004). The median depths (in centimeters) to the epidural space were 6.13 cm, 6.42 cm, and 7.13 cm for 50-60 kg, 60-70 kg, and 70-80 kg groups, respectively, at L5-S1. Age and height were not significantly associated with the needle depth. Conclusions: There is a positive association between the BMI (and weight) and transforaminal epidural depth but not with age, sex, and height.

• Journal of Magnetics
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• 제21권2호
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• pp.266-272
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• 2016

Transcranial magnetic stimulation devices has been used mainly for diagnostic purposes by measuring the functions of the nervous system rather than for treatment purposes, and has a problem of considerable energy fluctuations per repeated pulse. The majority of strokes are caused by ischemia and result in brain tissue damage, leading to problems of the central nervous system including hemiparesis, dysfunction of language and consciousness, and dysfunction of perception. Control is difficult and the size is large due to the difficulty of digitalizing the energy stored in a capacitor, and there are many heavy devices. In addition, there are many constraints when it is used for a range of purposes such as head and neck diagnosis, treatment and rehabilitation of nerve palsy, muscle strengthening, treatment of urinary incontinence etc. Output stabilization and minimization of the energy variation rate are required as the level of the transcranial magnetic stimulation device is dramatically improved and the demand for therapeutic purposes increases. This study developed a compact, low cost transcranial magnetic stimulation device with minimal energy variation of a high repeated pulse and output stabilization using a real time capacitor charge discharge voltage. Ischemia was induced in male SD rats by closing off the common carotid artery for 5 minutes, after which the blood was re-perfused. In the cerebrum, the number of PARP reactive cells after 24 hours significantly decreased (p < 0.05) in the TMS group compared to the GI group. As a result, TMS showed the greatest effect on necrosis-related PARP immuno-reactive cells 24 hours after ischemia, indicating necrosis inhibition, blocking of neural cell death, and protection of neural cells.

• 수산해양기술연구
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• 제60권3호
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• pp.244-257
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• 2024

Considering the negative impact of IUU fishing on fishery resources and fishery management, a revised approach for estimating risks of the ecosystem-based fisheries assessment (EBFA) of Zhang et al. (2011) was developed that incorporates three components of the IUU (illegal, unreported and unregulated) fishing as penalties. In this study, we introduced ways to develop indicators of IUU fishing suitable for the Korean fishery and apply them to ecosystem-based resource assessment. The indicator for the illegal fishing component was set as the fishing without licenses or permits, and that for the unreported fishing component was set as unreported fishing activities. Indicators for the unregulated fishing component were set as fishing operated by illegal fishing gear, illegal fish capture, fishing operations in prohibited fishing area, and fishing with restrict permits. IUU fishing significantly impacts the stock of target species. Therefore, in this study, the influence of IUU fishing is included in the Species Risk Index (SRI) at the species level, and weights are assigned based on the ratio of the stock, as $SRI={{\sum\limits_j}{\lambda}_j{\cdot}ORI/{\sum\limits_j}{\lambda}_j}+P_f(B_i/B)$. The revised ecosystem-based fisheries assessment method, which considers the impact of IUU fishing, was applied to major fisheries on the south coast of Korea. It is necessary to reduce the non-reporting rate through the expansion of the TAC system and improve the accuracy of statistical compilation. To this end, the electronic fishing reporting system, which is being implemented on all vessels in Korean distant water fishing vessels, should be introduced to the coastal and offshore fisheries as well.

• Journal of Radiation Protection and Research
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• 제18권1호
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• pp.63-70
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• 1993

$^{125}I$가 0.035 MeV 준위로 붕괴할 때 K-각 전자포획붕괴에 의한 K-각 이중 이온화 현상에 대하여, 이중 vacancy가 채워지면서 방출되는 $K_{\alpha}^{II}$ X-선과 $K_{\alpha}^s$X-선을 동시계수하여 연구 분석하였다. 실험에 사용한 source는 $^{125}I$차 $^{125}Te^m$의 혼합시료이다. 한 대의 Ge(Li)검출기, 두 대의 NaI(T1)섬광검출기와 TPHC(Time-to-Pulse Height Converter)를 사용하여 동시스펙트럼을 분석한 다음 측정된 동시계수 $N(K_{\alpha}^{II},\;K_{\alpha}^s)$과 $K_{\alpha}$ X-선의 총 수인 $N(K_{\alpha})$을 얻었다. K-자 전자포획 당 이중 Vacancy가 형성될 때의 이중 이온화 확률 $P_{KK}$값, $2.15{\times}10^{-4}$을 구하였다.

• 한국유기농업학회지
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• 제23권4호
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• pp.897-909
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• 2015

우리나라 포도원에서 고품질 포도생산에 이용되고 있는 잡초관리 방법들이 병발생 및 수체생육과 과실품질에 미치는 영향을 구명하여 포도재배 농가에 실제적인 잡초관리 방법을 제공하고자 연구를 실시하였다. '캠벨얼리' 품종을 재배하는 비가림 포도원에서 잡초관리방법으로 포도가지 파쇄(C.B.), 흑색부직포(W.F.), 흑색차광망(S.N.), 흑색비닐(P.F.), 왕겨(R.H.), 기계제초 2회(M.W.2), 기계제초 3회(M.W.3)를 무처리와 비교하였다. 비가림 포도원의 피복처리구(C.B., W.F., S.N., P.F., R.H.)와 무피복처리구(M.W.2, M.W.3, U.T.)간 평균온도는 각각 $26.2^{\circ}C$와 $25.8^{\circ}C$로 비슷하였으나, 피복처리구의 지상부 습도는 피복처리구에서 7월에 90%로 유지되다가 7월 하순 이후 40% 이하로 급격히 낮아져 무피복처리구와 확연한 차이를 보였다. 토양수분은 무피복처리구에 비해 피복처리구에서 모두 높았으나 지온은 대체적으로 피복처리구가 낮았다. 피복처리별 갈색무늬병 발병은 흑색비닐피복에서 무처리 대비 7월에는 85%, 병발생이 높아지는 8월에는 69%의 감소효과를 보였고, 결과모지에서 병반 발생이 가장 적은 것으로 나타났다. 생육특성 중 절간장, 절간경은 무처리에 비해 흑색비닐 피복에서 생육이 좋았고 SPAD값은 흑색비닐 피복에서 가장 높았다. 과실품질 중 당도는 $14{\sim}15^{\circ}Brix$로 큰 차이는 없었으나 흑색비닐 피복에서 약간 높게 나타났다.

• Biomolecules & Therapeutics
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• 제25권3호
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• pp.266-271
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• 2017

Synthetic cannabinoids are one of most abused new psychoactive substances. The recreational use of abused drug has aroused serious concerns about the consequences of these drugs on infection. However, the effects of synthetic cannabinoid on resistance to tetanus toxin are not fully understood yet. In the present study, we aimed to determine if the administration of synthetic cannabinoids increase the susceptibility to tetanus toxin-induced motor behavioral deficit and functional changes in cerebellar neurons in mice. Furthermore, we measured T lymphocytes marker levels, such as CD8 and CD4 which against tetanus toxin. JWH-210 administration decreased expression levels of T cell activators including cluster of differentiation (CD) $3{\varepsilon}$, $CD3{\gamma}$, CD74p31, and CD74p41. In addition, we demonstrated that JWH-210 induced motor impairment and decrement of vesicle-associated membrane proteins 2 levels in the cerebellum of mice treated with tetanus toxin. Furthermore, cerebellar glutamatergic neuronal homeostasis was hampered by JWH-210 administration, as evidenced by increased glutamate concentration levels in the cerebellum. These results suggest that JWH-210 may increase the vulnerability to tetanus toxin via the regulation of immune function.