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Antioxidative-activity of Phellinus baumii Pilát in Caenorhabditis elegans (상황버섯의 예쁜꼬마선충 내의 항산화 효과)

  • Kim, Jun Hyeong;Kwon, Kang Mu;Yang, Jae Heon;Ki, Byeolhui;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.50 no.4
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    • pp.299-304
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    • 2019
  • In order to research the anti-oxidative activity of methanol extract of Phellinus baumii Pilat (Hymenochaetaceae), Caenorhabditis elegans model system was used. Ethyl acetate soluble fraction of the methanol extract showed the most potent DPPH radical scavenging activity. The ethyl acetate fraction was measured on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance with reactive oxygen species (ROS) level in C. elegans. Furthermore, to see if regulation of stress-response gene is responsible for the increased stress tolerance of C. elegans which treated by the ethyl acetate fraction, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of P. baumii increased SOD and the catalase activities in a dose-dependent manner in C. elegans, reduced ROS accumulation dose-dependently. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the control worms.

A study on property of crystalline silicon solar cell for variable annealing temperature of SOD (SOD 온도 가변을 이용한 결정질 태양전지 특성 연구)

  • Song, Kyuwan;Jang, Juyeon;Yi, Junsin
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.124.1-124.1
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    • 2011
  • 결정질 태양전지에서 도핑(Doping)은 반도체(Semiconductor)의 PN 접합(Junction)을 형성하는 중요한 역할을 한다. 도핑은 반도체에 불순물(Dopant)을 주입하는 공정으로 고온에서 진행되며 온도는 중요한 변수(Parameter)로 작용한다. 본 연구에서는 여러 가지 도핑 방법 중 SOD(Spin-On Dopant)를 이용하여 온도에 따른 도핑 결과와 특성을 분석 하였다. P-type 웨이퍼(Wafer)에 SOD를 이용하여 불순물을 증착 후 Hot-plate에서 15분간 Baking 하였다. Baking된 웨이퍼는 노(Furnace)에 넣고 860C880C까지 10C씩 가변하였다. 각각의 조건에 대해 Lifetime과 Sheet Resistance을 측정하였고, 그 결과 880C에서의 Lifetime이 23.58μs860C에 비해 235.8% 증가하여 가장 우수 하였으며, Sheet Resistance 또한 68Ω/sq로 860C에서 가장 우수하게 측정되었다. SOD의 속도 가변에 따른 특성 변화를 보기 위해 온도는 880C에 고정한 후 속도를 3000rpm~4500rpm까지 500rpm간격으로 1시간동안 실험한 결과 rpm 속도에 따른 lifetime 변화는 거의 없었으며, Sheet Resistance는 3000rpm에서 63Ω/sq로 가장 우수 하였다. 본 연구를 통해 온도와 Spin rpm에 따른 특성을 확인한 결과 온도가 높을 때 Sheet Resistance가 가장 안정화 되며, lifetime이 더욱 우수한 것을 확인할 수 있었다.

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Zinc and Zinc Related Enzymes in Precancerous and Cancerous Tissue in the Colon of Dimethyl Hydrazine Treated Rats

  • Christudoss, Pamela;Selvakumar, R.;Pulimood, Anna B.;Fleming, Jude Joseph;Mathew, George
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.2
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    • pp.487-492
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    • 2012
  • Trace element zinc deficiency or excess is implicated in the development or progression of some cancers. The exact role of zinc in the etiology of colon cancer is unclear. To cast light on this question, an experimental model of colon carcinogenesis was applied here. Six week old rats were given sub cutaneous injections of DMH (30 mg/kg body weight) twice a week for three months and sacrificed after 4 months (precancer model) and 6 months (cancer model). Plasma zinc levels showed a significant decrease (p<0.05) at 4 months and a greater significant decrease at 6 months (p<0.01) as compared with controls. In the large intestine there was a significant decrease in tissue zinc levels (p<0.005) and in CuZnSOD, and alkaline phosphatase activity (p<0.05) in the pre-cancerous model and a greater significant decrease in tissue zinc (p<0.0001), and in CuZnSOD and alkaline phosphatase activity (p<0.001), in the carcinoma model. The tissue zinc levels showed a significant decrease in the small intestine and stomach (p<0.005) and in liver (p<0.05) in the cancer model. 87% of the rats in the precancer group and 92% rats in the cancer group showed histological evidence of precancerous lesions and carcinomas respectively in the colon mucosa. This study suggests that the decrease in plasma zinc, tissue zinc and activity of zinc related enzymes are associated with the development of preneoplastic lesions and these biochemical parameters further decrease with progression to carcinoma in the colon.

Effects of Pseudomonas aureofaciens 63-28 on Defense Responses in Soybean Plants Infected by Rhizoctonia solani

  • Jung, Woo-Jin;Park, Ro-Dong;Mabood, Fazli;Souleimanov, Alfred;Smith, Donald L.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.4
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    • pp.379-386
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    • 2011
  • The objective of this work was to investigate the ability of the plant growth-promoting rhizobacterium Pseudomonas aureofaciens 63-28 to induce plant defense systems, including defense-related enzyme levels and expression of defense-related isoenzymes, and isoflavone production, leading to improved resistance to the phytopathogen Rhizoctonia solani AG-4 in soybean seedlings. Seven-day-old soybean seedlings were inoculated with P. aureofaciens 63-28, R. solani AG-4, or P. aureofaciens 63-28 plus R. solani AG-4 (P+R), or not inoculated (control). After 7 days of incubation, roots treated with R. solani AG-4 had obvious damping-off symptoms, but P+R-treated soybean plants had less disease development, indicating suppression of R. solani AG-4 in soybean seedlings. Superoxide dismutase (SOD) and catalase (CAT) activities of R. solani AG-4-treated roots increased by 24.6% and 54.0%, respectively, compared with control roots. Ascorbate peroxidase (APX) and phenylalanine ammonia lyase (PAL) activities of R. solani AG-4-treated roots were increased by 75.1% and 23.6%, respectively. Polyphenol oxidase (PPO) activity in soybean roots challenged with P. aureofaciens 63-28 and P+R increased by 25.0% and 11.6%, respectively. Mn-SOD (S1 band on gel) and Fe-SOD (S2) were strongly induced in P+R-treated roots, whereas one CAT (C1) and one APX (A3) were strongly induced in R. solani AG-4- treated roots. The total isoflavone concentration in P+Rtreated shoots was 27.2% greater than the control treatment. The isoflavone yield of R. solani AG-4-treated shoots was 60.9% less than the control.

Effect of Hizikia fusiforme Extracts on Antioxidant Enzyme Activity and Vitamin E Concentration in Rats (톳 추출물의 경구투여가 흰쥐의 항산화효소 활성과 비타민 E 농도에 미치는 영향)

  • Kim, Hyang-Suk;Choi, Eun-Ok;Park, Cheol;Choi, Yung-Hyun;Hyun, Sook-Kyung;Hwang, Hye-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.11
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    • pp.1556-1561
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    • 2011
  • The purpose of this study was to investigate antioxidant enzyme activity and vitamin E concentrationin in Sprague-Dawley rat after being fed various extracts of Hizikia fusiforme. There were six experimental groups: control group (C), H. fusiforme ethanol extract group (EtOH), H. fusiforme dichloromethane fraction group (CH2Cl2), H. fusiforme ethylacetate fraction group (EtOAc), H. fusiforme butanol fraction group (n-BuOH), H. fusiforme water fraction group (H2O). H. fusiforme extracts (400 mg/kg B.W) were orally administrated to the rats every day for 4 weeks. The activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), and concentrations of malondialdehyde (MDA) and vitamin E in the liver and blood were measured. The activity of SOD in the liver was significantly higher in the CH2Cl2 and H2O groups (p<0.05) than in the control and other extract groups. The SOD activity in serum increased significantly in all H. fusiforme groups (p<0.05) compared to the control group and it was also significantly higher in the EtOH and H2O groups (p<0.05) than in other extract groups. The serum catalase activity increased significantly in the n-BuOH group (p<0.05) compared to the control and other extract groups. The plasma MDA concentration decreased significantly in the n-BuOH and H2O group (p<0.05) compared to the control group. Serum concentration of α-tocopherol showed no significant differences in most of the experimental groups, but it was significantly higher in the EtOAc group (p<0.05). The α-tocopherol concentrations in the liver showed a significant increase in the CH2Cl2 and H2O groups (p<0.05) compared to the control and other extract groups. The liver γ-tocopherol concentrations in H. fusiforme extract groups showed a tendency to increase compared to the control group and it was significantly higher in the H2O group (p<0.05) than in other extract groups. These results suggest that supplementation of water extracts of H. fusiforme extract could be effective in improving the antioxidant system.

Effects of Squalene on the Mice Hepatotoxicity Induced by Lead Acetate (납으로 유발된 생쥐 간장 독성에 대한 스쿠알렌의 효과)

  • Kim, Jong-Se
    • Applied Microscopy
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    • v.33 no.3
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    • pp.205-214
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    • 2003
  • To investigate the effect of the squalene against the lead toxicity. A healthy male of ICR mice were used for experiment. The SOD was observed after the intraperitoneal injection in mice. The ultrastructural changes of the liver were observed after the intraperitoneal injection in mice. The experimental groups were divided into two groups. Group A was control group that squalene was not treated after intraperitoneal injection of lead acetate. Group B was squalene treatment group that squalene solution was injected after intraperitoneal injection of lead acetate. All groups were used to 10 mice. The results were as follow: SOD activity in the liver, Group A was lower than in normal. But, Group B was higher than in Group A (P<0.05). In the histological observation, Group A were showed that the inner cavity of mitochondria swellen and development of cristae weakened. Swelling of lamellae of rough endoplasmic reticulum (rER) was showed. It was concluded that the SQ will be effective for the recovery of hepatic cell at lead intoxication.

Physiological Activities of Agaricus bisporus Extracts as Affected by Solvents (용매에 따른 양송이 추출물의 생리활성 효과)

  • Lim, Tae-Soo;Do, Jeong-Ryong;Kwon, O-Jun;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.4
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    • pp.383-388
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    • 2007
  • Physiological activities of Agaricus bisporus extracts were examined. Nitrite-scavenging activity, superoxide dismutase (SOD)-like activity and electron-donating ability (EDA) of Agaricus bisporus extracted with water, 50%, and 100% ethanol were measured. Nitrite- scavenging activities at pH 1.2 were the most effective in water and 50% ethanol extracts from brown and white Agaricus bisporus samples. SOD-like activities of water and 50% ethanol extracts of both samples were 19.0255.42 lower than those of 1.0% and 0.1% L-ascorbate solutions. SOD-like activities of brown Agaricus bisporus extracts were higher than those of white Agaricus bisporus extracts, and water extracts of the samples were the highest. EDAs of brown Agaricus bisporus extracts were higher (40.3566.54 than those of white Agaricus bisporus extracts, while those of both extracts were lower than 1.0% and 0.1% L-ascorbate solutions. The results will be useful for understanding the physiological activities of Agaricus bisporus extracts.

Physiological Activities of Mistletoe Extracts from Viscum album L. (추출용매에 따른 겨우살이 추출물의 생리활성 효과)

  • Ju, Min-Jeong;Do, Jeong-Ryong;Kwon, Joong-Ho;Kim, Hyun-Ku
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.5
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    • pp.529-534
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    • 2009
  • Physiological activities of mistletoe extracts were examined. Total polyphenol contents, electro-donating ability, SOD-like activity, nitrite-scavenging ability and tyrosinase of mistletoe extracted with water, 50%, and 100% ethanol were determined. Total polyphenol contents of powder extracts were higher than slice extracts. EDAs showed over 90% at powder extracts. Especially mistletoe extracts with 50% ethanol were higher than water extracts. SOD-like activities of water, 50% and 100% ethanol extracts of all samples were 23.7133.4 lower than those of 1.0% and 0.1% L-ascorbate solutions. Nitrite-scavenging activities at pH 1.2 were the most effective in water, 50% and 100% ethanol extracts. The results will be useful for understanding the physiolosical activities of mistletoe extracts.

Expression Pattern of Antioxidant Enzymes Genes in the Ventral Prostates of Rats Exposed to Procymidone and/or Testosterone after Castration

  • Lee, Jong-Geol;Yon, Jung-Min;Jung, Ki-Youn;Lin, Chunmei;Jung, A-Young;Lee, Beom-Jun;Yun, Young-Won;Nam, Sang-Yoon
    • Journal of Embryo Transfer
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    • v.26 no.4
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    • pp.265-270
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    • 2011
  • Procymidone is a fungicide with anti-androgenic properties widely used to protect fruits from fungal infection, which induces an excessive reactive oxygen species production in male reproductive organs. In this study, to clarify whether procymidone affect the cellular antioxidant system of prostate at onset of puberty, gene expression patterns of the representative antioxidant enzymes such as cytoplasmic glutathione peroxidase (GPx1), phospholipid hydroperoxide GPx (PHGPx), selenoprotein P (SePP), cytoplasmic copper/zinc superoxide dismutase (SOD1), and manganese SOD (SOD2) were investigated in the rat ventral prostates exposed to procymidone using real-time RT-PCR analyses. Seven-week-old Sprague-Dawley rats castrated at 6 weeks old were treated with procymidone (25, 50, or 100 mg/kg per day) orally for 7 consecutive days after testosterone propionate (0.4 mg/kg per day) administration by subcutaneous injection. As compared to normal control animals, GPx1 mRNA expression in prostates significantly increased by the administration with TP and/or procymidone. However, PHGPx and SOD1 mRNA levels significanatly decreased by over 25 mg/kg of procymidone treatment and SePP and SOD2 mRNA levels was significanatly reduced by over 50 mg/kg of procymidone treatment. These findings indicate that procymidone may affect the antioxidant system of prostatic cells in up-regulation mode of GPx1, but in down-regulation modes of PHGPx, SePP, SOD1, and SOD2, suggesting that procymidone may affect differently the cellular antioxidant system of prostate according to the exposure doses.

Changes on the Antioxidant Activities of Extracts from the Ziziphus jujube Miller Fruits During Maturation (성숙도에 따른 대추(Ziziphus jujube Miller) 추출물의 항산화 활성의 변화)

  • Hong, Ju-Yeon;Nam, Hak-Sik;Shin, Seung-Ryeul
    • Food Science and Preservation
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    • v.17 no.5
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    • pp.712-719
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    • 2010
  • This study was carries out to analyzed the antioxidant activities and xanthine oxidase inhibitory effects of extracts from jujube to provide basic data for the development of functional materials. Antioxidative activities of extracts from jujube were analyzed by electron donating ability (EDA) using 1,1-diphenyl-2-picryl hydrazyl (DPPH), superoxide dismutase (SOD)-like activity by pyrogallol and nitrite scavenging ability. Extract yields from jujube fruits were 11.55% for unripe fruits, and about twice that value when ripe fruit extracts were prepared. The yields of hot-water and ethanol extracts was 55.67 and 65.95% in dried fruits, respectively. Total phenol contents were higher in unripe fruit extracts. The EDA values of hot-water and ethanol extracts from jujube fruits were increased by increase of extract concentration, and were about 90% in 10.0 mg/mL of extract concentration. The SOD-like activity was increased by the increase of extract concentrations. The SOD-like activity of the hot-water extract from unripe fruits was higher than that of other extracts. The SOD-like activity of ethanol extracts was 39.92% at 10 mg/ml of extract concentration from unripe fruits. The nitrite scavenging ability was about 50% in 1.0 mg/ml of extract concentration at pH 1.2, and that of extracts from unripe fruits was higher than that of other extracts. The xanthine oxidase inhibitory activities of hot-water and ethanol extracts from unripe fruits were higher than those of other extracts, were increased by concentration of extracts.