• Title/Summary/Keyword: $PO{_4}^{3-}-P$

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Nutrient Leaching from Leaf Litter of Cropland Agroforest Tree Species of Bangladesh

  • Hasanuzzaman, Md.;Hossain, Mahmood
    • Journal of Forest and Environmental Science
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    • v.30 no.2
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    • pp.208-217
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    • 2014
  • Leaf litter is the main and quick source of organic matter and nutrient to the soil compared to other parts of litter. This study focused on the nutrients (N, P and K) leaching from leaf litter of Melia azadirachta, Azadirachta indica, Eucalyptus camaldulensis, Swietenia macrophylla, Mangifera indica, Zizyphus jujuba, Litchi chinensis, Albizia saman, Artocarpus heterophyllus, Acacia auriculiformis, Dalbergia sissoo and Khaya anthotheca as the common cropland agroforest tree species of Bangladesh. About (9 to 35) % of initial mass was lost, while Electric Conductivity (EC) and TDS (Total Dissolved Solid) of leaching water increased to (573 to 3,247) ${\mu}S/cm$ and (401 to 2,307) mg/l respectively after 192 hours of leaching process. Mass loss (%) of leaf litter, EC and TDS of leaching water showed significant (ANOVA, p<0.05) curvilinear relationship with leaching time. Initial concentration of NH4, PO4 and K in leaching water was found to increase significantly (p<0.05) up to 48/72 hours and then remained almost constant at later stages (48/72 to 192 hours). Mass loss of leaves; EC, TDS, $NH_4$, $PO_4$ and K in leaching water was varied also significantly (ANOVA, p<0.05) among the studied tree species. All the tree species showed similar pattern of nutrients (K>N>P) release during the leaching process. The highest $NH_4$ (4,097 ppm) and potassium (8,904 ppm) concentration was found for M. azadirachta while the highest $PO_4$ (1,331 ppm) concentration was found for E. camaldulensis in the leaching water. Among the studied tree species, M. azadirachta, A. indica, D. sissoo, E. camaldulensis and Z. jujuba was selected as the best tree species with respect to nutrient leaching.

A Study on the Cultural Characteristics of Pholiota nameko Mycelium (맛버섯 균사체의 배양 특성에 관한 연구)

  • 차월석;이동병;강시형;오동규
    • Journal of Life Science
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    • v.13 no.4
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    • pp.498-504
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    • 2003
  • This Study was carried out to investigate the optimal mycelial growth of Pholiota nameko. The optimal medium for the mycelial growth was ME medium. The optimal temperature and pH were $25^{\circ}C$$\pm$1 and 5.5, respectively. The modified optimal medium compositions were glucose 3% (w/v), malt extract 0.25% (w/v), yeast extract 0.25% (w/v), $KH_2PO_4$ 0.046% (w/v), $K_2HPO_4$ 0.1% (w/v), $MgSO_4$$7H_2O$ 0.05% (w/v). From the result of experiments on the optimal temperature, pH and nutritional requirements, the mycelial growth of modified optimal medium was higher than that of ME medium.

Nutrient Behavior in an Upland Field of Cabbage Adjacent to the River (하천변 양배추 밭에서의 영양물질의 거동)

  • Song, Chul-Min;Kim, Jin-Soo;Jang, Hoon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.52 no.3
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    • pp.65-71
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    • 2010
  • This study was conducted to investigate the dynamics of nutrients such as total nitrogen (TN), nitrate nitrogen ($NO_3$-N) total phosphorous (TP), and phosphate phosphorous ($PO_4$-P) in outflow from a cabbage farmland in a mixed land-use watershed. The TN concentrations in groundwater showed twice peaks in late July 2006 and late March 2007 (3.8, 4.7 mg/L, respectively), when it rained shortly after fertilizer application, indicating that nitrogen leaching is greatly influenced by fertilization and rainfall. The mean concentrations of TN and $NO_3$-N in surface water were not significantly higher than those in groundwater, while the mean concentrations of TP and $PO_4$-P in surface water were significantly (p < 0.05) were higher than those in groundwater. The TN concentrations in groundwater were generally higher than those in surface water during fertilization and early growing season due to the effect of fertilization, but vice versa in the other periods. In contrast, the TP concentrations in groundwater were always lower than those in surface water due to the sorption of particulate phosphorous by soil. The ratio of TN load in baseflow to that in total TN load (39 %) was much greater than the TP ratio (7 %), suggesting that baseflow contribute to nitrogen export. Therefore, proper fertilization management should be taken to reduce nitrogen load through baseflow.

Changes of water Quality During the Seed Production Period of Dark-banded Rockfish Sebastes inermis in Large Scale Tanks (대형 수조에서 볼락 종묘 생산에 따른 수질 환경의 변화)

  • Oh, Sung-Yong;Noh, Choong-Hwan
    • Journal of Aquaculture
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    • v.19 no.1
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    • pp.25-32
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    • 2006
  • An experiment was carried out to investigate changing of water quality during the seed production of dark-banded rockfish Sebastes inermis in large scale tanks. Ten broodstock of dark-banded rockfish were held in three circular tanks (diameter 6.5 m; depth 2 m; water volume 50 ton) each (stocking density $0.061kg/m^3$). During the experiment the temperature ranged from 14.2 to $16.1^{\circ}C$. The fingerlings were 134 with rotifers only during 1 to 9 days after parturition, rotifers with Artemia nauplii during 10 to 20 days after parturition, Artemia nauplii only during 21 to 35 days after parturition, Artemia nauplii with commercial diet during 36 to 80 days after parturition and commercial diet only during 81 to 85 days after parturition. Water quality (dissolved oxygen, pH, $NH_4^+-N,\;NO_2^--N,\;NO_3^--N\;and\;PO_4^{3-}-P$) in rearing tanks measured every 5 days in long term monitoring investigation or every 2 hours in diurnal monitoring investigation. In 85 days after parturition, the body weight of fish grew up to 0.88 f and specific growth rate was 8.0%/day in body weight. In long term monitoring investigation, with the increase of the amount of supplied commercial diet, the concentration of dissolved oxygen (DO) and pH decreased, but the concentration of $NH_4^+-N\;(4.5\;to\;76.3{\mu}M),\;NO_2^--N\;(0.02\;to\;0.06{\mu}M),\;NO_3^--N\;(3.0\;to\;5.9{\mu}M)$, and $PO_4^{3-}-P\;(0.41\;to\;0.59{\mu}M)$ increased. In the diurnal monitoring investigation, the concentration of $NH_4^+-N$ showed great fluctuation and ranged from 3.0 to $9.1{\mu}M$ when fed rotifers, 16.3 to $45.8{\mu}M$ when fed Artemia nauplii and 36.5 to $120.1{\mu}M$ when fed commercial diet. After daily feeding with each of feed, the amount of dissolved inorganic nitrogen (DIN) and phosphorus (P) wastage were 7.0 g and 0.7 g when fed rotifers, 24.7 g and 0.7 g when fed Artemia nauplii and 140.9 g and 2.2 g when 134 commercial diet. The amount of DIN and phosphorous wastage during 134 commercial diet was significantly higher than that of fed rotifer and Artemia nauplii (P<0.05). Results will provide valuable information far water quality management and culture of dark-banded rockfish in commercial seed production systems.

Development of Optimal Nutrient Solution of Tomato(Lycopercicon esculentum Mill.) in a Closed Soilless Culture System (순환식 수경재배에 적합한 토마토 배양액 개발)

  • Yu, Sung-Oh;Bae, Jong-Hyang
    • Journal of Bio-Environment Control
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    • v.14 no.3
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    • pp.203-211
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    • 2005
  • The experiment was conducted to investigate the nutrition absorption pattern in the growth stages and develope the optimal nutrient solution hydroponically grown the tomato in closed substrate culture system with the nutrient solution of National Horticultural Research Station in Japan into 1/2S, 1 S, and 2S. When plant was grown in 1/2 S, the growth and yield were high and the pH and EC in the rooting zone were stable. Suitable composition of nutrient solution for tomato was $NO_3-N$ 7.1, $PO_{4}$-P 2.1, K 4.0, Ca 3.1, Mg 1.2, and $SO_{4}-S\;1.2\;me{\cdot}L^{-1}$ in the early growth stage and $NO_3-N$ 6.5, $PO_4-P$ 2.3, K 3.4, Ca 3.1, Mg 1.1, and $SO_4-S\;1.1\;me{\cdot}L^{-1}$ in the late growth stage by calculating a rate of nutrient and water uptake. To estimate the suitability for the nutrient solution of tomato in a development of optimum nutrient solution of tomato developed by Wonkwang university in korea (WU), plant was grown in perlite substrate supplied with different solution and strengths(S) by research station for greenhouse vegetable and floricultuin in the Netherlands (Proefstation voor tuinbouw onder glas te Naaldwijk; PTG) of 1/2 S, 1 S and 2 S, respectively, The growth was good at the PTG and WU of 2 S in early growth stage, and at the WU 2S in late growth stage. The highest yield of tomato obtained in the WU of 2 S, although blossom-end rot was appeared in all treatments. pH and EC in root zone of WU of 2 S were stable during the early and late growth stage. Therefore when plant was grown in WU of 2 S, N and P content in the nutrient solution need to low, according N and P content of their leaves were high in WU of 2 S.

Optimization of Major Culture Elements on Growth and Shikonin Production in the Lithospermum erythrorhizon Hairy Root Culture

  • Hwang, Ok-Jin;Kim, Yu-Jeong;Sung, Nak-Sul;Ahn, Jun-Cheul;Kim, Sik-Eung;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.4
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    • pp.243-248
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    • 2002
  • The effects of basal media, carbon, nitrogen, phosphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM $KNO_3$ and 1 mM $NaH_2PO_4)$, whereas the highest shikonin production was in the condition supplemented with 5 mM $KNO_3$ and 10 mM $NaH_2PO_4$.

Relationships between arterial and urinary $P_CO_2}, P{O_2}$ and acid-base balances (동맥혈 및 뇨 $P_CO_2}, P{O_2}$ 의 산-염기 균형 및 뇨량과의 관계)

  • Kim, Yong-Jin;Lee, Yeong-Gyun
    • Journal of Chest Surgery
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    • v.16 no.2
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    • pp.213-220
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    • 1983
  • Pulmonary function is the determinant of blood gas tension. However, Acid-Base disturbances can also alter partial pressures of oxygen and carbon dioxide in arterial blood. During respiratory acidosis $PO_2$ will be lowered and reverse changes will be produced during respiratory alkalosis. On the other hand, in metabolic acidosis $PO_2$ will be elevated and $PCO_2$ will be lowered by the respiratory compensation, and reverse response will be induced in metabolic alkalosis. Urinary gas tension has many influencing factors than arterial blood and difficult to estimate the tendency of its alterations. Urinary $PO_2$ and $PCO_2$ are not always identical level as venous blood. It is to be altered by blood gas tension, flow rate of urine, metabolic rate of kidney, and Acid-Base status of blood. Particularly countercurrent exchange of oxygen and carbon dioxide in the renal medulla will make larger alteration of gas tension than venous blood. After induction of Acid-Base disturbances [disturbances] arterial and urinary $PCO_2$, $PO_2$, urinary volume, and osmolarity were determined in dogs, and the relationships between arterial and urinary $PCO_2$ , $PO_2$ Acid-Base disturbances, urinary volume, and osmolarity were investigated. 1. During the acute Metabolic and Respiratory disturbances urinary pH did not respond on respiratory origin. However, there were immediate urinary response in pH on metabolic origin. 2. Urinary $PO_2$, $PCO_2$, did not always follow arterial or venous gas tension and Acid-Base disturbance. Urinary $PCO_2$, correlate well with the urinary volume. The larger the urinary volume, $PCO_2$ lowered to the venous level. The smaller the urinary volume, urinary $PCO_2$ tends to be higher. However urinary $PO_2$ did not have any particular correlation with urinary volume. 3. Correlation between urinary $PCO_2$ and $PO_2$ were inversely proportional to arterial blood. Differences of $PCO_2$ between arterial blood and urine also did not have any particular correlation with urinary volume. This may suggest that changes on blood gas tensions can influence on urinary $PCO_2$. 4. There were eminent clear inverse correlation between urinary $PCO_2$ and osmolar concentrations of urine. Above results strongly suggest that partial pressure of gas in urine primarily depend upon counter-current exchanges in renal medullary tissues.

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Comparison of shear bond strength according to porcelain build-up methods (도재 축성 방법에 따른 금속 도재관의 전단결합강도 비교)

  • Lee, Ha-Young;Cho, Jin-Hyun;Lee, Cheong-Hee
    • Journal of Dental Rehabilitation and Applied Science
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    • v.30 no.2
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    • pp.112-120
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    • 2014
  • Purpose: This study compared the shear bond strength of heat pressed and feldspathic porcelain to metal. Through thermocycling, the clinical aspect of heat pressed porcelain fused metal was estimated. Materials and Methods: 90 non-precious metal specimens were made ($4{\times}4{\times}8 mm$) and divided to three groups. All spicimens were treated and built-up with the porcelain ($4{\times}4{\times}3 mm$) by 2 different methods according to group: Group I: $Inspiration^{(R)}$, Group II: Ivoclar, IPS $Inline^{(R)}PoM$, Group III: GC Initial IQ-One $Body^{(R)}PoM$. The half of each group's specimens were thermocycled. All specimens' shear bond strength were measured by Instron universal testing machine. Exact measuring point was far 1 mm from porcelain/metal interface to the porcelain side. For the statistical analysis, 2-way ANOVA was used. Results: In no-thermocycling specimens, the shear bond strength showed no statistical significance between each group (P > 0.05). In comparison between nothermocycling and thermocycling specimens in each group, the shear bond strength was decreased according to thermocycling, but there was no statistical significance (P > 0.05). In thermocycling specimens, there was no statistical significance between each group (P > 0.05). Conclusion: In feldspathic porcelain and other two types heat pressed porcelain, there was no statistical difference in the shear bond strength of porcelain to metal. The heat pressed porcelain seems to be clinically useful for the aspect of the shear bond strength.

Optimal Conditions for the Laccase Production from Fomitopsis pinicola Mycelia (Fomitopsis pinicola 균사체로부터 Laccase의 최적생산조건)

  • Park, Naomi;Park, Sang-Shin
    • Microbiology and Biotechnology Letters
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    • v.37 no.1
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    • pp.62-68
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    • 2009
  • The culture conditions to maximize the production of laccase (EC 1.10.3.2) from Fomitopsis pinicola mycelia were investigated. Among the tested media for the enzyme production, mushroom complete medium (MCM ; 2% dextrose, 0.2% peptone, 0.2% yeast extract, 0.05% $KH_2PO_4$, and 0.05% $MgSO_4{\cdot}7H_2O$) showed the highest activity of the enzyme. To optimize the culture condition for the laccase activity, influence of various carbon and nitrogen sources was investigated in MCM. Among various carbon and nitrogen sources, 2% glucose and 0.4% peptone showed the highest production of the enzyme, respectively. For the phosphorus and inorganic source, 0.05% $NaH_2PO_4$ and 0.05% $CaCl_2$ were best for the enzyme activity. The enzyme production was reached to highest level after the cultivation for 8 days at $25^{\circ}C$. Native polyacrylamide gel electrophoresis (PAGE) followed by the laccase activity staining using 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the substrate was performed to identify the laccase under culture conditions studied. Zymogram analysis of the culture supernatant showed a laccase band with molecular mass of 52 kDa. The optimum pH and temperature for the enzyme activity were $80^{\circ}C$ and pH 3.0.

The Crystal Structure of Hydroazonium Diphosphate, $N_2H_6H_4(PO_4)_2$ (Hydrazonium Diphosphate, $N_2H_6H_4(PO_4)_2$의 結晶構造)

  • Koo, Chung-Hoe;Ahn, Choong-Tai;Kim, Sung-Ho
    • Journal of the Korean Chemical Society
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    • v.9 no.3
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    • pp.128-133
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    • 1965
  • Hydrazonium diphosphate crystallizes with the space-group symmetry $P2_1/C.$ There are two formular units of $N_2H_6H_4(PO_4)_2$ in the unit cell, for which $a = 4.52{\pm}0.02, b = 8.06{\pm}0.03, c = 10.74{\pm}0.03{\AA}\;and\; {\beta} = 100{\pm}0.5^{\circ}.$ The determination of the crystal structure was carried out by means of Patterson, Fourier and difference syntheses. The phosphate group has configuration of nearly regular tetrahedron with the mean P-O distance of $1.55{\AA}.$ The N-N distance found is $1.40{\AA},$ which corresponds to previously reported values for the $N_2H_6^{++} \;ion \;in\; N_2H_6SO_4.$ A molecule has a transform with a center of symmetry in it. Each nitrogen atom forms three hydrogen bonds with the N…O distances 2.62, 2.79 and $2.89{\AA}.$ And a O…O hydrogen bond between different phosphate groups is found with the distance $2.63{\AA}.$ The structure is held together by three-dimensional network of the strong hydrogen bonds.

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