• Journal of Applied Biological Chemistry
• /
• v.53 no.4
• /
• pp.189-194
• /
• 2010

We confirmed the hypo-osmotic shock strengths and duration, different type of vectors, and subcelluar localization to identify the optimum analysis condition of plant aquaporin activity in Xenopus ooctye using Arabidopsis thaliana AtPIP2-1 gene. Six minutes and 1/5ND buffer hypoosmotic shock treatment was the best condition to show the maximum swelling of Xenopus oocytes where AtPIP2-1 was expressed using pcDNA3.1 vector. AtPIP2-1 protein was expressed more efficiently in pGEMHE vector which has 5' and 3' UTR (untranslation region) of Xenopus ${\beta}$-GLOBIN gene in multiple cloning site than in pcDNA3.1 vector. Also green fluorescence of GFP fused to AtPIP2-1 was detected onto oocyte plasmamembrane where is the proper subcellular localization target of AtPIP2-1.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.34 no.4
• /
• pp.370-377
• /
• 2001

Flounder brain cytosol contains protein inhibitors that markedly inhibit the activity of partially purified brain membrane phospholipase D (PLD) which is dependent on phosphatidylinositol 4,5-bisphosphate ($PIP_2$) but insensitive to ADP-ribosylation factor (ARF), The PLD inhibitors have been enriched through several chromatographic steps and characterized with respect to size and mechanism of inhibition. Sequential chromatography of the brain cytosol yielded six inhibitor fractions, Two (IIA and IIB) of six inhibitor fractions showed the $PIP_2$-phosphatase activities. IIA was identified as synaptojanin, a nerve terminal protein that has known to be a member of the inositolpolyphosphate 5-phosphatase family, by immunoblot analysis. IIB showed an apparent molecular mass of 158 kDa by Superose 12 gel filtration chromatography and was immunologically distinct from synaptojanin. IIB hydrolyzed $PIP_2$, yielding only phosphatidylinositol phosphate (PIP) as product, suggesting that IIB hydrolyzes only one phosphate from either the 4- or 5-position of PI (4,5)$P_2$. These studies demonstrate that the existence of multiple $PIP_2$-phosphatases have been implicated in the negative regulation of $PIP_2$-dependent PLD activity within flounder brain.

• The Journal of Society for e-Business Studies
• /
• v.11 no.1
• /
• pp.113-126
• /
• 2006

The scope of e-Commerce process becomes wider as the emphasis on the enterprise collaboration grows. It has expanded from its initial settings of order management or billing processes to cover various collaborative processes in the company's value-chain. In order for those collaborative e-Commerce processes to be successful, corresponding business processes should be fully supported by standard bodies. The RosettaNet consortium, one of the most representative international B2B standard bodies, has steadily provided new PIPs to support those expansions. Since individual RosettaNet PIPs correspond to unit tasks that are executed separately in or between companies, multiple PIPs have to be integrally used to properly handle larger business cases. RosettaNet implementation, however, has suffered from the lack of standard guidelines or deliverables to refer under this multi-PIP environment. In this research, a contents-based document routing system is implemented. By applying this routing system to the RosettaNet e-Logistics program where multi-PIP environment is inevitable, we verified our contents-based document routing system is effective to support multi-PIP environment flexibly.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.56 no.3
• /
• pp.183-191
• /
• 2011

This study was undertaken to collect basic knowledge of Jatropha which is one of bio-energy crops, based on the understanding of physiological and molecular aspects under salt and drought conditions. The treatments were followed as: 100, 200 and 300 mM NaCl for salt stress and 5, 10, 20 and 30% PEG for drought stress for 8 days, respectively. Leaf growth, stomatal conductance, chlorophyll fluorescence and gene expression of aquaporin (JcPIP2) of Jatropha were investigated. From 2 days after treatments, plants treated with higher than 100 mM NaCl and 10% PEG respectively were significantly suppressed in leaf length, width, and stomatal conductance, but 5% PEG treatment showed that plant growth was improved more than control plant. Semi-quantitative RT-PCR analyses revealed that the JcPIP2 gene was expressed in root, stem, cotyledon and leaves. It was not detected in leaves at 200 and 300 mM NaCl treatments. However, transcripts of JcPIP2 were induced in roots and stems under salt and drought conditions compared to those of healthy plants. Therefore, it was concluded that JcPIP2 plays an important role in improving drought tolerance.

• BMB Reports
• /
• v.38 no.3
• /
• pp.360-365
• /
• 2005

Membrane inositol glycerophospholipid (IGP) is metabolized to phosphatidylinositol-4-phosphate (PIP), phosphatidylinositol-4, 5-bisphosphate ($PIP_2$), and inositol triphosphate ($IP_3$) in signaling transduction. This study was carried out to determine the subclasses of IGP involved in signaling pathway. The acyl chain moieties of the phospholipids are easily modulated by dietary fatty acids. We analyzed acyl chain composition of IGP 3-subclasses, PIP and $PIP_2$ from rat brain after feeding sunflower seed oil enriched with linoleic acid or fish oil high in eicosapentaenoic acid and docosahexaenoic acid. Long chain polyunsaturated fatty acids (LCPUFA) as eicosapentaenoic acid and docosahexaenoic acid were not incorporated into ether-linked IGP (alkenylacylglycerophosphoinositol and alkylacyl-glycerophosphoinositol), PIP and $PIP_2$, while diacyl-glycerophosphoinositol (GPI) contained high LCPUFA. These results suggest that PIP might be phosphorylated from only the ether-linked IGP (alkenylacyl- and alkylacyl species) but not from diacyl subclass for signals to intracellular responses in the plasma membrane of rat brain.

• BMB Reports
• /
• v.43 no.2
• /
• pp.127-132
• /
• 2010

Phosphatidylinositol (3,4,5)-triphosphate ($PIP_3$) is a lipid second messenger that employs a wide range of downstream effector proteins for the regulation of cellular processes, including cell survival, polarization and proliferation. One of the most well characterized cytoplasmic targets of $PIP_3$, serine/threonine protein kinase B (PKB)/Akt, promotes cell survival by directly interacting with nucleophosmin (NPM)/B23, the nuclear target of $PIP_3$. Here, we report that nuclear $PIP_3$ competes with Akt to preferentially bind B23 in the nucleoplasm. Mutation of Arg23 and Arg25 in the PH domain of Akt prevents binding to $PIP_3$, but does not disrupt the Akt/B23 interaction. However, treatment with phosphatases PTEN or SHIP abrogates the association between Akt and B23, indicating that nuclear $PIP_3$ regulates the Akt/B23 interaction by controlling the concentration and subcellular dynamics of these two proteins.

• 한국HCI학회:학술대회논문집
• /
• 2006.02a
• /
• pp.305-310
• /
• 2006

TV 프로그램 전송 방식이 아날로그에서 디지털로 바뀌며, TV방송의 다채널화 현상이 일어나고 있다. 동시에 많은 채널을 시청하며, 정보를 습득하고자 하는 바쁜 현대인들에게 두 개 이상의 채널을 동시에 시청하면서, 이를 녹화하거나 모니터링 하고자 하는 Need가 점점 높아져 가고 있다. 두 개의 튜너를 이용하여 두 채널의 방송을 동시에 시청 할 수 있는 PIP(Picture in Picture-동시화면) 기능은 이러한 사용자 욕구를 해소함과 동시에, 프리미엄 TV의 기술적 특성으로 자리잡고 있다. 현재 LG전자 TV의 경우, PIP관련 기능은 5가지로 [동시화면] [부채널+] [부채널 -] [부화면 입력][주부전환] 이 있다. 이러한 PIP관련 기능은 리모컨에서 부가기능 버튼으로 동작하고 있으며, 리모컨 레이아웃의 복잡성을 증대시키는 큰 원인이 되고 있다. 특히 관련 버튼이 숨김 버튼으로 배치되어 있어 사용자는 버튼을 찾지 못하고 오히려TV OSD 메뉴 상에서 PIP기능을 찾는 오류를 범하고 있어 직관적인 사용성을 제안하지 못하고 있다. 따라서 본 연구에서는 리모컨에서 PIP관련 버튼을 줄임과 동시에 PIP 사용성을 높일 수 있는 개선안을 제안하고자 하였다. 연구 방법은 PIP 사용행태 분석을 통한 문제점 파악 후 2가지 개선안을 도출한 후 각각의 개선안에 대한 사용성 검증을 실시하였다. 그 결과, 기존의 리모컨 버튼으로 접근하는 것이 사용성이 더 좋을 것이라는 예상을 깨고, PIP기능 사용 시 OSD메뉴로 접근하는 것이 수행시간이 줄어들었음을 알 수 있었다. 이것은 TV라는 매체의 특성상 사용자는 TV화면에서 기능 수행 후 Feedback을 기대하며, TV화면 상에서 동작하는 것에 더 익숙하기 때문이다. 이는 향후 새로운 TV기능 구현 시 중요한 Impact로 작용할 수 있다. 그 이유는 리모컨 버튼을 활용하여 모든 기능을 수행하는 것보다, 특정 기능은 OSD상에서 구현하는 것이 TV의 직관적인 사용 Scene에 더 적합한 것을 파악할 수 있었기 때문이다.

• The Korean Journal of Physiology and Pharmacology
• /
• v.27 no.2
• /
• pp.187-196
• /
• 2023

Transient receptor potential canonical (TRPC) channels are non-selective calcium-permeable cation channels. It is suggested that TRPC4β is regulated by phospholipase C (PLC) signaling and is especially maintained by phosphatidylinositol 4,5-bisphosphate (PIP₂). In this study, we present the regulation mechanism of the TRPC4 channel with PIP₂ hydrolysis which is mediated by a channel-bound PLCδ1 but not by the G_qPCR signaling pathway. Our electrophysiological recordings demonstrate that the Ca²⁺ via an open TRPC4 channel activates PLCδ1 in the physiological range, and it causes the decrease of current amplitude. The existence of PLCδ1 accelerated PIP₂ depletion when the channel was activated by an agonist. Interestingly, PLCδ1 mutants which have lost the ability to regulate PIP₂ level failed to reduce the TRPC4 current amplitude. Our results demonstrate that TRPC4 self-regulates its activity by allowing Ca²⁺ ions into the cell and promoting the PIP₂ hydrolyzing activity of PLCδ1.

• Archives of Plastic Surgery
• /
• v.36 no.4
• /
• pp.450-457
• /
• 2009

Purpose: The second toe PIP joint free flap is a method of reconstruction used for abnormalities of the PIP joint of the finger. We report the results of the additional tenolysis in patients with a difference between passive ROM and active ROM after second toe PIP joint free flap. Methods: From March 2001 to July 2008, tenolysis was performed in patients with a difference in their active and passive ROM after second toe PIP joint free transfer, performed on 14 fingers. We performed a retrospective analysis of the medical records, noting the clinical and radiological findings. In addition, we measured the preoperative and postoperative range of motion of the PIP joint. Results: The average active ROM was $22.5^{\circ}$ at the three months after the joint transfer surgery, and was $38^{\circ}$ after additional tenolysis between five months and twelve months after the joint transfer. Conclusions: Additional tenolysis, after the second toe PIP joint free flap, might be a good option for improved results in patients with difference in active and passive ROM of a transferred PIP joint.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.14 no.12
• /
• pp.953-958
• /
• 2001

The antifuse is a semi-permanent memory device like a ROM which shows the open or short state, and a switch device connecting logic blocks selectively in FPGA. In addition, the antifuse has been used as a logic device to troubleshoot defective memory cells arising from SDRAM processing. In this study, we have fabricated ONO antifuses consisted of PIP structure. The antifuse shows a high resistance more than several G Ω in the normal state, and shows a low resistance less than 500 Ω after program. The program resistance variation according to temperature shows the very stable value of $\pm$20 Ω. At this time, its program voltage shows 6.7∼7.2 V and the program is performed within 1 second. Therefore this result shows that the PIP antifuse is a very stable and programmable logic device.