This study was conducted to compare the storability of 6 chicory cultivars for producing chicon; 'Vintor', 'Focus', 'Metafora', 'Kibora', 'Nobus', and 'Redoria Red' grown in 2 regions: Chuncheon (plain region) and Pyeongchang (high land region). Chicons were produced from chicory roots that grown for 120days and then stored for over 120days at $2^{\circ}C$ and 90% of RH conditions. To produce chicon, chicory root was forced at $18^{\circ}C$ for 22days with suppling the nutrient solution ($KNO_30.54g{\cdot}L^{-1}$, $Ca(NO_3)_2\;1.02g{\cdot}L^{-1}$, $MgSO_4\;0.36g{\cdot}L^{-1}$, $KH_2PO_4\;0.21g{\cdot}L^{-1}$, $K_2SO_4\;0.10g{\cdot}L^{-1}$, pH 7.0). Chicons produced from 6 different chicory cultivars packed with $25{\mu}m$ ceramic film and stored for 25days at $8^{\circ}C$. The fresh weight of chicon in MAP was maintained to 99.5% of pre-storage weight. The fresh weight of 'Redoria Red' was lowest in all cultivars, and that of Chuncheon region cultivated treatment was lower than Pyeongchang treatment. The $CO_2$ and $O_2$ concentration in chicon MAP were 2% and $10{\sim}17%$. There were not significantly different among cultivars and between regions, although 'Redoria Red' cultivar showed highest $CO_2$ and lowest $O_2$ concentrations. The ethylene concentration in chicon MAP was $1.0{\mu}{\iota}{\cdot}{\iota}^{-1}$ and also didn't show any significant difference among all treatments. Chicon detoriorated visual quality with appearing russet spotting that result from ethylene gas. The visual quality of 'Redoria Red' cultivar decreased faster than the other cultivars. 'Metafora', 'Focus', and 'Kibora' maintained higher firmness of their leaf than the others, and the firmness was higher grown in Pyeongchang region cultivated treatments than in Chuncheon region.
Head-down tilt (HDT) at $-6^{\circ}$ has been commonly used as the experimental model in both man and animals to induce the blood shift toward the head or central protion of the body, demonstrating similar physiological effect encountered in the weightlessness in the orbital flight. There are few reports about the physiological response upon the cardiovascular regulatory system or the tolerance to the $(-6^{\circ})$ HDT within a relatively short period less than 1 hour. Therefore, the purpose of this study way to observe the effects of $-6^{\circ}$ HDT on cardiovascular system within 30 minutes and to evaluate early regulatory mechanism for simulated hypogravity. Ten mongrel dogs weighing 8-12 kg were anesthetized with the infusion of 1% ${\alpha}-chloralose$ (100 mg/kg) intravenously, and the postural changes were performed from the supine to the $-6^{\circ}$ head-down Position, then from the head-down to the supine (SUP), and each posture was maintained for 30 minutes. Blood flow $({\dot{Q}})$ through common carotid and femoral arteries were determined by the electromagnetic flowmeter. Mean arterial pressure (MAP), heart rate (HR), respiratory rate , and pH, $P_{O_2}$, $P_{CO_2}$ and hematocrit (Hct) of arterial and venous blood were also measured. The peripheral vascular resistance was calculated by dividing respective MAP values by ${\dot{Q}}$ through both sides of common carotid or femoral arteries. The concentration of plasma epinephrine and norepinephrine was determined by Peuler & Johnson's radioenzymatic method. The results are summarized as follows: In the initial 5 minutes in $-6^{\circ}$ HDT, HR was significantly (p<0.05) increased and MAP slightly decreased. Although ${\dot{Q}}$ and carotid peripheral artery resistance were not significantly changed, ${\dot{Q}}$ through femoral artery was diminished and femoral peripheral artery resistance was elevated. In the SUP, the initial changes of MAP and HR were increased (p<0.05), but those of ${\dot{Q}}$ and peripheral vascular resistance through both common carotid and femoral arteries were not significant. After 10 minutes of each postural change in both HDT and SUP, MAP was maintained almost equal to that of the level of pretilting control. During 60 minutes of both postural changes of HDT and SUP, $P_{O_2}$ and Hct were not changed significantly. However pH tended to increase slowly and $P_{CO_2}$ was gradually decreased. The pH and $P_{CO_2}$ seemed to be related to the increased respiratory rate. Plasma epinephrine concentration was not changed significantly and plasma norepinephrine concentration was slightly decreased in the course of HDT and also at 10 minutes of SUP. However these concentration changes were statistically insignificant. From these results, it may be concluded that the effect of $-6^{\circ}$ HDT for 30 minutes on the cardiovascular system and plasma catecholamine levels of the dog is minimum and it is suggestive that the cardiovascular regulatory mechanism, possibly mediated by so called gravity receptors including baroreceptor and volume receptor, has been properly and adequately operated.
Certain amino acids are essential precursors of a variety of important biomolecules in addition to their major function as protein building blocks. ${\delta}$-Aminolevulinic acid (ALA) is synthesized from the condensed form of succinyl-CoA with glycine after decarboxylation catalyzed by ALA synthase. The objective of the study was to determine the effects of ALA supplementation on growth performance, behavioral characteristics and hematological/immune status in nursery pigs. A total of 144 pigs weaned at 21 d of age were allotted to three dietary treatments representing (-) control (w/o antibiotics; NC), (+) control (w/carbadox at 50 mg/kg; PC), and the treatment group with ALA supplementation (0.05%; TA). Each treatment had 6 pens (replicates) with 8 pigs per pen. Pigs were fed phase 1 (21.9% CP, 1.40% Lys) and 2 (20.6% CP, 1.15% Lys) experimental diets for 3 and 2 wks, respectively. Feed intake and weight gain were measured weekly during phase 1 and at the end of phase 2. At the end of phase 2, blood samples were taken and analyzed using an automated hematology analyzer. Skin color and activity of pigs (48 h) from all pens in each treatment were measured at the second week of phase 2. Growth performance was not affected (p>0.05) by the dietary supplementation of ALA during the 5 wk nursery period. Pigs in the TA (6.46) and PC (6.68) had a higher (p<0.05) number of red blood cells ($10^6cell/{\mu}L$) than pigs in the NC (6.15). Pigs in PC (12.16) had a higher (p<0.05) hemoglobin level (g/dL) than pigs in the NC group (11.29) and the TA group (11.47). Pigs in the TA and PC had darker (p<0.05) and less (p<0.05) yellow skin color than pigs in the NC. Pigs in the PC tended (p = 0.081) to be less active than pigs in the other groups. There were no differences in behavioral characteristics between the NC and the TA. The data suggest that ALA supplementation has no adverse effects on growth performance of nursery pigs. Moreover, ALA supplementation increased red blood cell counts which may be beneficial to pigs.
Park, Chol-Soo;Jung, Yeong-Sang;Joo, Jin-Ho;Yang, Jae-E
Korean Journal of Soil Science and Fertilizer
/
v.37
no.2
/
pp.66-73
/
2004
As one of the typical farming practices in the sloped upland in Pyeongchang and Hongcheon area, application of piling with coarse saprolite materials has been practiced by farmers for several reasons such as reduction of damage by monocropping, better development of plant roots, and better drainage. However, adverse effect on application of coarse saprolite soil materials to environmental aspects should not be ignored. Therefore, this research was conducted to evaluate the physicochemical properties of coarse saprolite materials in upland fields in Pyeongchang area. According to particle size distribution of coarse saprolite materials, averaged gravel contents for Pyeongchang and Hongcheon county were 16.7 and 25.3%, respectively. There was no significant difference in gravel contents by soil depth, and CV values for each particle size ranged from 20 to 40%, which implied that application of coarse material with similar properties. When we compared CEC values of dressed soil with or without considering gravel content, CEC values decreased as increasing gravel contents. The penetration resistances were 0.04-7.48 MPa at the 0 to 10 cm surface soil, and 0.10 to 8.80 MPa at the depth below 11 cm. The bulk density of the soil was $1.15g\;cm^{-3}$ at the surface soil and 1.29 to $1.35g\;cm^{-3}$ at the soil depth below 10 cm. The organic matter content, cation exchange capacity, and avaliable $P_2O_5$ concentrations of soil in upland where piling with saprolite materials of Pyeongchang area applied were $12.4g\;kg^{-1}$, $7.1cmol_c\;kg^{-1}$, and $526mg\;kg^{-1}$, respectively. Cation exchange capacity was lower than that of averaged Korean upland soil, while available $P_2O_5$ concentration was relatively higher than that of averaged Korean upland, which indicated high input of various fertilizers.
Journal of the Korean Society of Food Science and Nutrition
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v.35
no.2
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pp.139-144
/
2006
In this study, we investigated the antioxidant activity of solvent extracts from Sargassum thunbergii by measuring electron-donating ability (EDA), nitrite- scavenging ability, superoxide dismutase (SOD)-like activity, reducing power and antioxidant activity in $\beta-carotene-linoleate$ model system. Total phenolic compound contents of chloroform and methanol extracts from Sargassum thunbergii were $22.5{\pm}0.8$ mg/100 mL and $20.1{\pm}0.6$ mg/100 mL, respectively. The EDA were proportionally increased with concentration of the extracts except hexane extract. The EDAs were $70.4\%\;and\;63.2\%$ in methanol and water at concentration of $1000{\mu}g/mL$, respectively. The nitrite-scavenging ability of methanol extract (pH 2.5, $1000{\mu}g/mL$) was $89.4{\pm}0.9\%$ that was higher than those of other extracts. SOD-like ability of chloroform extract was stronger than other extracts. Reducing power of chloroform extracts at $1000{\mu}g/mL$ were 0.75 as O.D. value of 700 nm and antioxidant activities measured with $\beta-carotene-linoleate$ model system were in order of methanol $(78.4\%)>BHT\;(72.1\%)>chloroform\;(62.2\%)>water\;(51.4\%)>hexane\;(43.2\%)$. These results indicated that methanol extract from Sargassum thunbergii showed the highest activities on the EDA, nitrate- scavenging ability and antioxidant activities while the chloroform extract had the highest effect on the SOD-like activity and reducing power.
Lee, J.I.;Lee, J.D.;Ha, Y.J.;Jung, J,D.;Lee, J.W.;Lee, J.R.;Kwack, S.J.;Kim, D.H.;Do, C. H.
Journal of Animal Science and Technology
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v.46
no.6
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pp.1013-1022
/
2004
This study investigated the effects of silkworm droppings(SWD) added diet feeding on chemical composition of pork loin meat. Pigs were divided into 7 treatment groups(10 pigs/group) and subjected to one of seven treatment diets(O, 1 0/0, 2 0/0, 3% SWD for 4 weeks, 1 0/0, 2 0/0, 3% SWD for 8 weeks, SWD diets; total fed diets) before slaughter. Pork loin were collected from the animals(1l0 kg body weight) slaughtered at a commercial slaughter house. Pork loin meat were aerobic packaged and then stored at$4^{\circ}C$ for 2, 5, 8, or 12 days. Samples were analyzed for TBARS, sensory evaluation, myoglobin content, fatty acid composition, amino acid and mineral content. TBARS value was not significantly different between the control and SWD treatment groups. Myoglobin content of SWD fed group pork was significantly increased than that of control group(P<0.05). Marbling score and acceptability were increased in SWD fed pork compared to those in control pork. Na, Mg, Ca, Mn and Fe content of SWD fed group pork was significantly decreased than those of control group(P<0.05). P content was significantly increased than that of control group(P< 0.05). In the change of amino acid composition, the contents of aspartic acid, serine and tyrosine were decreased by dietary SWD-supplementation, whereas the SWD-supplementation resulted in the higher glutamic acid, alanine and isoleucine. As dietary SWD was increased in feed, the content of oleic acid was significantly increased, but stearic acid was significantly decreased(P<0.05).
Background : Idiopathic pulmonary fibrosis (IPF) is a diffuse inflammatory and fibrosing process that occurs within the interstitium and alveolus of the lung with invariably poor prognosis. The major problem in management of IPF results from the variable rate of disease progression and the difficulties in predicting the response to therapy. The purpose of this retrospective study was to evaluate the short-term efficacy of steroid and immunosuppressive therapy for IPF and to identify the pre-treatment determinants of favorable response. Method : Twenty patients of IPF were included. Diagnosis of IPF was proven by thoracoscopic lung biopsy and they were presumed to have active progressive disease. The baseline evaluation in these patients included clinical history, pulmonary function test, bronchoalveolar lavage (BAL), and chest high resolution computed tomography (HRCT). Fourteen patients received oral prednisolone treatment with initial dose of 1mg/kg/day for 8 to 12 weeks and then tapering to low-dose prednisolone (0.25mg/kg/day). Six patients who previously had experienced significant side effects to steroid received 2mg/kg/day of oral cyclophosphamide with or without low-dose prednisolone. Follow-up evaluation was performed after 6 months of therapy. If patients met more than one of followings, they were considered to be responders : (1) improvement of more than one grade in dyspnea index, (2) improvement in FVC or TLC more than 10% or improvement in DLco more than 20% (3) decreased extent of disease in chest HRCT findings. Result : One patient died of extrapulmonary cause after 3 month of therapy, and another patient gave up any further medical therapy due to side effect of steroid. Eventually medical records of 18 patients were analyzed. Nine of 18 patients were classified into responders and the other nine patients into nonresponders. The histopathologic diagnosis of the responders were all nonspecific interstitial pneumonia (NSIP) and that of nonresponders were all usual interstitial pneumonia (UIP) (p<0.001). The other significant differences between the two groups were female predominance (p<0.01), smoking history (p<0.001), severe grade of dyspnea (p<0.05), lymphocytosis in BAL fluid ($23.8{\pm}16.3%$ vs $7.8{\pm}3.6%$, p<0.05), and less honeycombing in chest HRCT findings (0% vs $9.2{\pm}2.3%$, p<0.001). Conclusion : Our results suggest that patients with histopathologic diagnosis of NSIP or lymphocytosis in BAL fluid are more likely to respond to steroid or immunosuppressive therapy. Clinical results in large numbers of IPF patients will be required to identify the independent variables.
There has recently been increasing interest in the use of NMDA receptor antagonists as potential neuroprotective agents for the treatment of ischemic stroke. To evaluate the neuroprotective effect of the selective non-competitive NMDA receptor antagonist MK-801 in focal cerebral ischemia, local cerebral glucose utilization (ICGU) was examined in 15 neuroanatomically discrete regions of the conscious rat brain using the 2-deoxy-D[$^{14}C$] glucose quantitative autoradiographic technique 24 hr after left middle cerebral artery occlusion (MCAO). Animals received MK-801 (5 mg/kg i.v.) or saline vehicle before (20-30 min) or after (30 min) MCAO. Both pretreatment and posttreatment of MK-801 increased occluded/non-occluded ICGU ratio in 7 and 5 of the 15 regions measured, respectively (most notably in cortical structures). Following MK-801 pretreatment, there was evidence of widespread increases in ICGU not only in the non-occluded hemisphere (12 of the 15 areas studied) but also in the occluded hemisphere (13 of the 15 areas studied), while MK-801 postreatment did not significantly increase ICGU both in the normal and occluded hemispheres. These data indicate that MK-801 has a neuroprotective effect in focal cerebral ischemia and demonstrate that MK-801 provides widespread alterations of glucose utilization in conscious animals.
Journal of the Korean Society of Food Science and Nutrition
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v.44
no.1
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pp.111-117
/
2015
This study investigated quality characteristics of commercial frozen chopped garlic. We collected six kinds of commercial frozen chopped garlic, four of which were domestic and the remaining two made in China. The moisture contents and drip loss of domestic frozen chopped garlic were significantly lower than those of Chinese garlic. Drip loss was positively correlated with moisture content (r=0.918, P<0.01). Total pyruvate contents of frozen chopped garlic ranged from $118.69{\pm}5.97{\sim}224.81{\pm}9.92{\mu}mol/g$ in domestic garlic and $75.27{\pm}5.91{\sim}79.35{\pm}1.79{\mu}mol/g$ in Chinese garlic. Allicin contents of frozen chopped garlic ranged from $8.91{\pm}0.34{\sim}13.09{\pm}0.35mg/g$ in domestic garlic and $5.91{\pm}0.08{\sim}6.05{\pm}0.27mg/g$ in Chinese garlic. Total thiosulfinate content of commercial frozen chopped garlic ranged from $0.60{\pm}0.07{\sim}1.33{\pm}0.12$ optical density/g. Total thiosulfinate content of commercial frozen chopped garlic was positively correlated with allicin content (r=0.892, P<0.01). Flavor patterns of commercial frozen chopped garlic measured by an electronic nose showed no difference between domestic and Chinese garlics.
This study was conducted to figure out the environment factors including vegetation structure and soil characteristics in natural habitats of Sageretia thea, and offers the basic information for habitats conservation and proliferation. The natural habitats of Sageretia thea were located at altitudes between 0~370 m with inclinations ranged as 3~35°. Through the vegetation research, the dominant species of tree layers were found to be divided into four communities. Cornus macrophylla (Com. I), Pinus thunbergii - Cinnamomum camphora (Com. II), Machilus thunbergii (Com. III), and Pinus thunbergii (Com. IV). The Species diversity (H') was 1.397~1.455, evenness (J') was 0.972~0.986, and dominance (D) was found to be 0.014~0.028. As a result of the physicochemical characteristics of soils, habitats soil mainly consisted of sandy soil and sandy loam soil. The average soil pH was 5.28~5.98, electronic conductivity was 0.22~63 ds/m, soil organic matter was 13.33~19.33 cmol+/kg, Exchange cations were appeared in the order of Ca2+, Mg2+, K+, and Na+. The Ordination result showed that Correlation coefficient between communities and environmental factors were significantly correlated with 4 main factors altitude, electronic conductivity, cation exchange capacity, exchangeable Na+. As expected, The result of this study will be helpful information on the preservation and mass production for use.
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