• Bulletin of the Korean Chemical Society
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• v.32 no.4
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• pp.1158-1164
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• 2011

5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILICMS/MS). Chromatographic separation was achieved on an Atlantis HILIC silica column ($5{\mu}m$, $100{\times}2.1\;mm$). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 1-1000 ng/mL with linear correlation co-efficients ($r^2$) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.4
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• pp.259-264
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• 2000

This study was conducted to investigate the effects of copper in the efficiencies of callus formation and plant regeneration of orchardgrass (Dactylis glomarata L.). Seeds were cultured on MS medium containing $2\;mg/{\ell}$ 2,4-D and different concentrations ($0.1-100\;{\mu}mol$) of copper sulfate. Plant regeneration was achieved on N6 medium containing $1\;mg/{\ell}$ NAA, $5\;mg/{\ell}$ kinetin and $0.1-100\;{\mu}mol$ of copper sulfate. Callus formation was not affected by copper incorporation into MS medium. However, the efficiency of plant regeneration was promoted by copper and the maximum efficiency was obtained when $70\;{\mu}mol$ copper was incorporated in the culture medium. The average number of regenerated plants from the seed-derived callus was also increased by copper.

• Journal of the Korean Society of Marine Environment & Safety
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• v.14 no.4
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• pp.267-273
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• 2008

The purpose of this study is to investigate the distributive property of organic pollutants in effluents of argo/industrial wastewater treatment plants in Jeonnam using simultaneous analysis method of 310 chemicals. The numerous organic pollutants were detected in four sampling sites and the major chemicals were pesticides, CH type chemicals such as polycyclic compounds, CHO type chemicals such as phthalates, and CHO(N) type chemical such as aromatic amines. Moreover, 17 kinds of endocrine disrupters which include diethylptbalate were detected in each sampling sites. TU (Toxic unit) indicated cytotoxicity of samples using XTT assay, it appeared highly at A point(27.2) and D point(24.4). Also, the results from the correlation between total concentration of pollutants and TU did not correspond to the results of chemical and biological analysis.

• Journal of Korea Multimedia Society
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• v.13 no.11
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• pp.1687-1697
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• 2010

The USIM-based AKA authentication process is essential to a mobile payment system on smart phone environment. In this paper a payment protocol and an AKA module are designed for mobile payment system which is suitable for openness smart phone environment. The payment protocol designs the cross authentication among components of the mobile payment system to improve the reliability of the components. The AKA module of mobile payment system based on 3GPP-AKA protocol prevents the exposure of IMSI by creating the SSK(Shared Secure Key) through advance registration and solves the SQN(SeQuence Number) synchronization problem by using timestamp. Also, by using the SSK instead of authentication vector between SN and authentication center, the existing bandwidth $(688{\times}N){\times}R$ bit between them is reduced to $320{\times}R$ bit or $368{\times}R$ bit. It creates CK and IK which are message encryption key by using OT-SSK(One-Time SSK) between MS and SN. In addition, creating the new OT-SSK whenever MS is connected to SN, it prevents the data replay attack.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.1-6
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• 1999

In order to obtain plantlet derived by anthers, the anthers of Lilium asiatic hybrid 'Gran Paradiso' were cultured on Murashige and Skoog's medium supplemented with various combinations of auxin and cytokinin. The most suitable pollen stage of anther culture for the callus induction was 3 days before anthesis at the early to late binucleate stage. Organogenic calli were induced on MS medium supplemented with 5.0 mg/L 2,4-D alone and the combination of 1.0 mg/L 2,4-D and 1.0 mg/L kinetin, however, the combination of NAA and BA was more effective than that of 2,4-D and kinetin on plant regeneration through organogenesis. Shoots were formed from the induced callus on the medium with 0.5 mg/L NAA and 1.0 mg/L BA after 180 days of culture. Multiple shoots with 3-4 leaves, roots, and bulblets were formed on the medium with the combination of 2.0 mg/L NAA and 2.0 mg/L BA after 250 days of culture. The chromosome from root tip of the regenerated plantlet showed the diploid (2n=2x=24). Diploid plants were transferred to the pots and all plants were flowered in two years.

• Analytical Science and Technology
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• v.9 no.4
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• pp.392-398
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• 1996

Eleven organic chemical substances, tetrachloroethylene, ethyl benzene, p-xylene, o-xylene, isopropyl benzene, n-propyl benzene. 1,2,4-trimethyl benzene, 1,3,5-trimethyl benzene, p-isopropyl toluene. see-butyl benzene, and naphthalene, which have hazardously influenced to human, were extracted from untreated wastewater collected at 26 companies of 8 types industry in the basin of Kwangju stream. Their structures were elucidated by Gas Chromatography/Mass Spectrometry(GC/MS) and in comparison with each standard reagents.

• The Korean Journal of Physiology and Pharmacology
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• v.10 no.2
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• pp.71-77
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• 2006

The goal of this study was to analyze the effects of genistein, a widely used tyrosine kinase inhibitor, on cloned Shaw-type $K^+$ currents, Kv3.1 which were stably expressed in Chinese hamster ovary (CHO) cells, using the whole-cell configuration of patch-clamp techniques. In whole-cell recordings, genistein at external concentrations from 10 to $100{\mu}M$ accelerated the rate of inactivation of Kv3.1 currents, thereby concentration-dependently reducing the current at the end of depolarizing pulse with an $IC_{50}$ value of $15.71{\pm}0.67{\mu}M$ and a Hill coefficient of $3.28{\pm}0.35$ (n=5). The time constant of activation at a 300 ms depolarizing test pulses from -80 mV to +40 mV was $1.01{\pm}0.04$ ms and $0.90{\pm}0.05$ ms (n=9) under control conditions and in the presence of $20{\mu}M$ genistein, respectively, indicating that the activation kinetics was not significantly modified by genistein. Genistein $(20{\mu}M)$ slowed the deactivation of the tail current elicited upon repolarization to -40 mV, thus inducing a crossover phenomenon. These results suggest that drug unbinding is required before Kv3.1 channels can close. Genistein-induced block was voltage-dependent, increasing in the voltage range $(-20\'mV{\sim}0\'mV)$ for channel opening, suggesting an open channel interaction. Genistein $(20{\mu}M)$ produced use-dependent block of Kv3.1 at a stimulation frequency of 1 Hz. The voltage dependence of steady-state inactivation of Kv3.1 was not changed by $20{\mu}M$ genistein. Our results indicate that genistein blocks directly Kv3.1 currents in concentration-, voltage-, time-dependent manners and the action of genistein on Kv3.1 is independent of tyrosine kinase inhibition.

• The Korean Journal of Medicine
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• v.99 no.1
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• pp.50-56
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• 2024

Nocardiosis is uncommon. Immunocompromising conditions predispose individuals to pulmonary and disseminated nocardiosis of the brain, skin, and subcutaneous tissues. The most common pathogens are Nocardia cyriacigeorgica, Nocardia nova, and Nocardia farcinica. The speciation of Nocardia to determine antimicrobial susceptibility is difficult using traditional biochemical methods. Here, we report the case of a 73-year-old man with chronic obstructive lung disease who developed a rapidly progressing intramuscular abscess around the left hip and thigh. Within 3 days, the lesions progressed to an epidural abscess at the L4 to S1 level. Although he was treated with broad-spectrum antibiotics and extensive incision and drainage, he died of rapidly progressive respiratory failure. Nocardia abscessus (N. abscessus) was identified in pus samples using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This case shows that the diagnosis of an intramuscular abscess caused by N. abscessus is challenging and that using MALDI-TOF MS may facilitate the diagnosis and ensure appropriate treatment.

• Korean Journal of Applied Biomechanics
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• v.34 no.3
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• pp.133-144
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• 2024

Objective: Neuromuscular training may often result in an incomplete improvement of biomechanical risk factors associated with non-contact ACL injury. This study aimed to investigate the effects of reactive neuromuscular training (RNT) on dynamic postural control of the lower extremity during single-leg drop landing (SLDL) in comparison with conventional neuromuscular training (CNT). Method: Twenty-eight recreational female athletes were randomized to a CNT group (n=15) or a RNT group (n=13). All participants performed SLDL for three-dimensional motion analysis before and after CNT and RNT. The kinematic variable was calculated from before 50 milliseconds (ms) to after 100 ms initial contact: joint angle (degree). The kinetic variable was calculated from initial contact to after 100 ms: internal net joint moment (Nm/kg). Time series curve for normalized data was represented for group means and associated 95% confidence intervals. Effect sizes using Cohen's d was also calculated. Results: In within-group differences, the CNT group showed decreased knee flexion angle (0~100% of the task) and decreased knee valgus angle (0~50% of the task) after the intervention, whereas the RNT group showed increased hip flexion angle (0~14% of the task), decreased hip adduction angle (0~100% of the task), and decreased knee valgus angle (0~100% of the task) after the intervention. In between-group differences, the RNT group exhibited more hip flexion angle (0~49, 61~91% of the task), more knee flexion angle (0~100% of the task), and less knee valgus angle (0~55% of the task) than the CNT group. Conclusion: These findings suggest that both training intervention programs modified dynamic posture of the hip and knee during SLDL. Therefore, combining a neuromuscular training program with RNT technique would be more efficient for future injury prevention efforts.

• The Korean Journal of Food And Nutrition
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• v.24 no.4
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• pp.617-621
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• 2011

$Coptis$ $chinensis$ Franch rhizome is one of the important traditional Korea medicines with anti-inflammatory, anti-bacterial, anti-hypertensive and anti-cancer properties. The methanol extract of rhizome from the $Coptis$ $chinensis$ rhizome was purified by using preparative centrifugal partition chromatography (CPC) and HPLC method. The optimum two-phase CPC solvent system was composed of n-butanol:acetic acid:water at a ratio of 4:1:5. Berberine (16.8 mg) was successfully isolated by CPC and HPLC. The chemical structure of the compound was identified by (1 H)$^1H$, $^{13}C$-NMR and ESI-MS spectral data analysis.